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INDONESIA
Majalah Patologi Indonesia
ISSN : 02157284     EISSN : 25279106     DOI : https://doi.org/10.55816/
Core Subject : Health,
Majalah Patologi Indonesia (MPI) digunakan sebagai wahana publikasi hasil penelitian, tinjauan pustaka, laporan kasus dan ulasan berbagai aspek di bidang patologi manusia. Tujuannya ialah menghadirkan forum bagi permakluman dan pemahaman aneka proses patologik serta evaluasi berbagai penerapan cara diagnostik sejalan dengan kemajuan perkembangan ilmu dan teknologi. Selain itu juga untuk merangsang publikasi barbagai informasi baru/mutakhir.
Articles 643 Documents
Ekspresi Bcl-2 pada Karsinoma Urotelial Kandung Kemih dan Hubungannya dengan Berbagai Faktor Prognosis Hendy Setyo Yudhanto; Budiana Tanurahardja; Puspita Eka Wuyung
Majalah Patologi Indonesia Vol. 28 No. 3, September 2019
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

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Abstract

BackgroundUrothelial carcinoma comprises of 90% of all cases in bladder. There are two groups which are non invasivedepend on whether the tumor has reached muscularis mucosa. 5-Years survival rate depend on grading andstaging. Low malignant grade and non invasive may reach 90% survival rate, with recurrence rate 40-60%. Highmalignant grade and invasive tumor has only10-17% survival rate. Mitosis and lymphovascular invasion arerelated with recurrency. However, there are some controvesi regarding Bcl-2 expression in bladder urothelialcarcinoma. This study was aimed to evaluate different expression of Bcl-2 with 4 related factors contributed tosurvival, which are degree of malignancy, stage, mitosis, and lymphovascular invasion.MethodsA retrospective cross sectional study on urothelial carcinoma of the bladder was conducted in the Departmentof Anatomical Pathology, Faculty of Medicine, University of Indonesia/RSCM. The cases were collected fromthe year of 2010 to 2014. In this study 21 cases of low grade and 21 cases of high high grade were found. Bcl-2immunohistochemistry staining performed in all cases and percentages are calculated to make 0-3 scoring.ResultsMost case is fifth decade as many as 27 cases. Obtained 4 cases found limfovaskuler invasion. This studyobtains the relationship between mitosis with grade of tumor (p: 0.000). the result suggests that there are nodifferences between the expression of Bcl-2 with the degree of malignancy stage, mitosis and lymphovascularinvasion.
Hubungan Ekspresi Matriks Metaloproteinase-9 dengan Jenis Histopatologi Karsinoma Serviks dan Invasi Limfovaskular Meta Oktora; Salmiah Agus; Pelsi Sulaini
Majalah Patologi Indonesia Vol. 28 No. 3, September 2019
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

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Abstract

BackgroundMatrix Metalloproteinase 9 (MMP-9) is a proteolytic enzyme assumed to have an important role in cervicalcarcinogenesis. Limfovascular invasion (LVI) and higher expression of MMP-9 will make cervical tissuedegradation faster, invasion process and metastasist easier. Adenocarcinoma is suspected of having poorprognostic than squamous cell carcinoma (SCC). The aim of this study was to examine the relationshipbetween expression of MMP-9 with histopathological cervical carcinoma and LVI.MethodsA cross sectional analytic study was performed on 36 sample that divided into 18 KSS and 18 adenokarsinoma.Samples were tooken from block paraffin from surgery or biopsy in Anatomic Pathology laboratories are locatedin West Sumatra. Sample are reviewed and found the LVI. Immunohistochemical was staining with primaryantibody MMP-9 then its expression were assessed in stroma and cytoplasmic cell tumour.ResultsMMP-9 expression was found positive in 80.6% (41.1% for SCC and 58.6% for cervical adenocarcinoma. Themean of expression of SCC was 17.4 and cervical adenocarsinoma was 19.56. Expression of MMP-9 waspositive in LVI sample.ConclusionThere is not significant relationship between the expression of MMP-9 with type of histopatologic cervicalcarcinoma (p=0.088) and LVI (p=0.566). Expression of MMP-9 in cervical adenocarcinoma was higher thanSCC, but it was not significant (p=0.563).
Akurasi Pemeriksaan Polymerase Chain Reaction Menggunakan Primer IS6110 dan MPB64 untuk Mendeteksi Mycobacterium tuberculosis pada Spesimen Formalin-Fixed Paraffin-Embedded Timotius Benedict Djitro; Faramitha Nur Izzaty; Nur Rahadiani
Majalah Patologi Indonesia Vol. 28 No. 3, September 2019
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

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Abstract

BackgroundOne of the chalangges faced in controlling tuberculosis (TB) is the availability of a fast and precise diagnostictool. The current gold standard for diagnosis of TB is acid-fast bacterial culture. However, FFPE (formalin-fixedparaffin-embedded) tissues are not ammenable for culture and the time required makes this method impracticalfor many centers. PCR is an alternative rapid diagnostic tool, and can be used with FFPE tissues. PCR hasshown high sensitivity and specificity for detecting M.tuberculosis, however there has been reports of lesssensitivity and specificity when using FFPE specimens. This report aims to determine the accuracy of PCRdetection of M.tuberculosis using primer IS6110 and MPB64 on FFPE specimens.MethodsLiterature searches were carried out on the Pubmed, Scopus, Proquest, Springer Link and Cochranedatabases. Two journals were relevant to the clinical scenario, then a critical review is carreid out using theDiagnostic Study Appraisal Worksheet from the Center for Evidence-based Medicine, University of Oxford,2010.ResultsCurrently, the accuracy of PCR primer IS6110 and MPB is not satisfactory. Sensitivity ranges from 45.5-88.9%and specificity is between 88.1% - 100. This broad sensitivity range is probably caused by tissue volume andembedding process that can cause damages to the integrity of M.tuberculosis DNA.ConclusionPCR examinations using IS6110 and MPB64 primers can be used as confirmation tests in FFPE specimensdue to its high specificity value. Broad sensitivity values can produce significant false negatives if no otherexamination is used in conjunction with PCR in FFPE specimens.
Hubungan Ekspresi Imunohistokimia Cyclooxygenase-2 (COX-2) dengan Derajat Histopatologi Meningioma Indri Mahrani; Delyuzar; Joko S. Lukito
Majalah Patologi Indonesia Vol. 28 No. 3, September 2019
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

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Abstract

BackgroundMeningioma is the most common intracranial primary neoplasm in adults, which arise from arachnoid cap(meningothelial) cells. Histological appearance of a meningioma is an important predictor of tumor behavior.Cyclooxygenase-2 (COX-2) enzyme is overexpressed in different types of tumors and is known to beassociated with malignant behavior of tumors. The aim of this study was to evaluate cyclooxygenase-2 (COX-2)immunoreactivity in meningioma and to find association with histopathologic grading of meningioma.MethodsThis is an analytical descriptive study with cross sectional approach. The histopathologic grading of 22 samplesof meningioma were determined and stained with COX-2 immunohistochemistry. The color intensity: 1 (mild), 2(moderate), 3 (strong). Percentage of positive cells: 0 (0%), 1 (<10%), 2 (10-50%), 3 (>50%). The evaluationwas the sum of color intensity and percentage of positive cells: positive (>4), negative (<4).ResultsTwnty two cases of meningioma: mean age=41 years, 90.9% females, meningothelial=22.7%, grade I=77.3%).COX-2 expression: negative: 17 cases (77,3%) all was first degree of malignancy. COX-2 positive: 5 cases(22,7%): 4 cases of second degree and 1 case of third degree of malignancy. The expression of COX-2 inmeningioma cells was associated with WHO histopathologic grading (p=0.001).ConclusionThis study showed a relationship between COX-2 immunohistochemical expression and histopathologic gradingof meningioma, where COX-2 was more expressed in grades II and III.
Ekspresi HPV16 Onkoprotein E7 pada Lesi Prakanker dan Karsinoma Sel Skuamosa Serviks Verli Saniba; Wresnindyatsih; Zulkarnain Musa; Zen Hafy
Majalah Patologi Indonesia Vol. 29 No. 2, Mei 2020
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v29i2.409

Abstract

BackgroundCervical cancer is the secondmost common malignancy in women worldwide.The vast majority of cervical cancersare associatedwith high risk HPV16 infection. The persistence of HPV16 and integration of viral DNA into cell genome are necessary for thedevelopment of precancerous lesion and squamous cell carcinoma (SCC) of the cervix. The integration of viral DNA in the host cellgenome resulted in the regulation of oncoprotein E7 leading to detection of HPV16 E7 expression in precancerous lesions and SCCof the cervix.The aim of this study was to evaluate differences of HPV16 E7 expression in precancerous lesions and cervical SCC.MethodsAn observational study with cross-sectional design was performedat Department of Anatomical Pathology Faculty of MedicineUniversity of Sriwijaya/dr. Mohammad Hoesin General Hospital Palembang, from 1st July 2014 to 30st Juni 2017. The selectedsamples consisted of 25 precancerous cases lesions (13 cases of LSIL and 12 samples of HSIL) and SCC (25 cases).Immuhistochemical stained was conducted using HPV16 E7 antibody. Fisher exact’s test was used to analyze differences inexpression of HPV16 E7 among precancerous lesions and cervical SCC.ResultsThe pracancerous lesions occurred mostly between the ages of 30-40 years, while SCC were those between the ages of 51-60years. All SCC cases (100%) and 91.6% of HSIL showed weak to strong HPV16 E7 expression, and 84.6% cases of LSIL showedimmunonegative.ConclusionThe positivity of HPV16 E7 increased in HSIL and KSS lesions.
Identifikasi Polimorfisme Gen CYP1A1 pada Karsinoma Sel Skuamosa dan Adenokarsinoma Serviks Hilda Fitriyani; Delyuzar; Hidayat
Majalah Patologi Indonesia Vol. 29 No. 2, Mei 2020
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v29i2.410

Abstract

BackgroundCervical cancer is the third most common cancer in women with risk factor of smoking, high parity, long term use of oralcontaception that are associated with chemical carcinogenesis. Chemical carcinogenesis require biotransfor-mation of lipophilicsubstrates to hydrophilic metabolites, therefore facilitating their secretion from the human body. Cytochrome P450 (CYP) is one ofgenes that have important role in this process. Benzo[α]pyrene and estrogen have a common biotransformation process which ismetabolized by CYP, particularly CYP1A1. The objectives to identify the frequency and distribution of CYP1A1 gene polymorphismin squamous cell carcinoma and adenocarcinoma of the cervix.MethodsThis is an analytical descriptive study with cross sectional approach. CYP1A1 gene polymorphism (3801T/C or Ile462Val) wasanalyzed using PCR-RFLP method followed by gel electrophoresis.ResultsCYP1A1 gene polymorphisms (3801TC) in squamous cell carcinoma were 50% heterozygote T/C, 36% wild-types T/T and 14%homozygote C/C. CYP1A1 gene polymorphisms (3801TC) in adenocarcinoma were 60% heterozygote T/C and 40% wild-types T/T.CYP1A1 gene polymorphisms (Ile462Val) in squamous cell carcinoma were 97.2% heterozygote Ile/Val, and 2.8% homozygoteVal/Val. CYP1A1 gene polymorphisms (Ile462Val) in adenocarcinoma were 100% heterozygote Ile/ValConclusionThe most common type of CYP1A1 gene polymorphism (3801TC and Ile462Val) in squamous cell carcinoma and adenocarcinomaof the cervix were heterozygote.
Peran CD44 pada Progresivitas Non Alcoholic Steatohepatitis (NASH) Sarwanti; Marini Stephanie; Ria Kodariah
Majalah Patologi Indonesia Vol. 29 No. 2, Mei 2020
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v29i2.415

Abstract

Non alcoholic Steatohepatitis (NASH) is part of a group of conditions called Non-Alcoholic Fatty Liver Diseases(NAFLD) where it is a chronic disease, which defined after elimination other causes of fatty liver, such as excessivealcohol consumption and other causes of chronic liver diseases. NASH is fatty liver disease which characterized byballooning of hepatocyte and lobular inflammation with or without fibrosis. Histopathology diagnose on NASH can bedefined by performing liver biopsy. The purpose of liver biopsy is to define level and degree of the disease. Cluster ofDifferentiation (CD) 44 is a transmembrane glycoprotein receptor which located on the surface of macrophage cells,lymphocytes and endothelial cells. In studies which conducted on mice and humans, showed that CD44 playsimportant role in the progression of NASH. CD44 regulates inflammation of adipose tissue and liver. CD44 ispresumed as a marker which increase macrophage infiltration and other inflammatory cells on liver. This processleads to ultimate increment on insulin resistance and fatty liver. Deficiency was discovered on mice which injectedwith methionine and choline deficiency diet (MCDD). CD44 is associated with preventive method to prevent livercomplication by reducing macrophage or monocyte and as well as neutrophil accumulation in liver which wasevaluated through reducing numbers of inflammatory focus, expression of inflammatory cytokines: tumor necrosisfactor α (TNFα), interleukin (IL) -1B and nitric oxide synthesis (iNOS), and pro-inflammatory types of macrophage. Inobese patients, number of CD44 is predicted to be increasing.
Hubungan Kepadatan Tumor Associated Macrophages CD-68 dan Karakteristik Klinikopatologi pada Karsinoma Payudara Invasif Miko Kresna Handayani; Heni Maulani; Nursanti Apriyani
Majalah Patologi Indonesia Vol. 29 No. 2, Mei 2020
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v29i2.417

Abstract

BackgroundBreast carcinoma is the most common malignancy among women worldwide. Many factors can influence prognosis of the disease.Tumor microenvironment (TME) is enriched in highly active immune cells. Herein, tumor associated macrophages (TAM) are themost prevalent immune cells. TAM can enhance tumor progression by promoting angiogenesis, invasion and metastasis. Somestudies demonstrate that high density of macrophages infiltration associated with poor prognosis. The aim of the study is todetermine the association between TAM density and clinicopathological characteristics of breast invasive carcinoma.MethodsDescriptive study with case series design on January 1, 2015-October 31, 2017 was performed at Department of AnatomicalPathology Faculty of Medicine University of Sriwijaya/dr. Moh. Hoesin General Hospital Palembang on 48 samples invasivecarcinoma mammae subtype unspecific, and have already staining with immunohistochemistry staining ER, PR, HER-2 and Ki-67,surrogate markers. Then immunohistochemistry staining using CD-68 antibody was conduced. The association between TAMdensity and clinicopathological characteristics were analyzed by using Spearman’s Rho test.ResultsThe majority of the patient are older than 40 years of age (77.1%), with positive lymphovascular invasion (79.2%), and at the gradeIII (62.5%). Bivariate analysis between TAM CD-68+density in stromal tumor and subtype luminal A showed negative correlationmoderate significantly (r= -0.398; p=0.005) while between TAM CD-68+density in stromal tumor and subtype triple negative showedpositive correlation moderate significantly (r=0.335; p=0.020).ConclusionTAM CD-68+density in stromal tumor was significantly correlated with luminal A and triple negative subtype.
Analisis Imunoekspresi Programmed Death Ligand 1 (PD-L1) dan Indeks Proliferasi Ki-67 pada Tumor Astrositik Difus Billy Parulian Lubis; Hasrayanti Agustina; Hermin Aminah Usman; Bethy Surjawathy Hernowo; Sri Suryanti; Ahmad Faried; Hendrikus Bolly
Majalah Patologi Indonesia Vol. 29 No. 2, Mei 2020
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v29i2.418

Abstract

BackgroundDiffuse astrocytic tumours (DAT) is a diffuse infiltrative astrocytoma tumor accompanied by molecular parameters in the form or absence ofIsocitrate Dehydrogenase (IDH) gene mutations. The DAT category without the examination of the IDH gene mutation is classified as NotOtherwise Specified (NOS). Ki-67 is a marker for DAT proliferation so that it can assess the degree of DAT. Diffuse astrocytic tumoursbehavior is influenced by proliferative ability and the presence of a tumor microenvironment. The tumor microenvironment PD-L1 factorinfluences DAT behavior and allows the development of target therapy in DAT cases. This study aimed to analyze the relationship betweenPD-L1 and Ki-67 imunoexpression in the histopathological degree of DAT.MethodsCross-sectional study of 30 paraffin blocks of DAT NOS cases from 2014-2018 in the Department of Anatomical Pathology RSUP dr. HasanSadikin, Bandung. The sample consisted of 15 cases of grade II, 7 cases of grade III and 8 cases of grade IV, carried outimmunohistochemical staining with GFAP, PD-L1 and Ki-67. Assessment of PD-L1 immunoexpression was carried out by the histoscoremethod. The Ki-67 assessment was assessed based on its distribution, which was <10% and ≥10%. PD-L1 and Ki-67 immunoexpressionwith histopathological degrees in DAT were statistically analyzed by Kolmogorof Smirnov test, however association betweenimmunoexpression of PD-L1 and Ki-67 were analyzed by chi-square test.ResultsThere was a significant relationship between PD-L1 immunoexpression and histopathological degree in DAT (p=0.005). There was asignificant relationship between Ki-67 immunoexpression and DAT histopathology degree and with PD-L1 (p=0.001 and p=0.010).ConclusionPD-L1 immunoexpression and proliferation rates affect the degree of DAT. Proliferation rate of DAT is influenced by PD-L1 so that in thisstudy, PD-L1 and Ki-67 can be used as a marker of prognosis predictors.
Analisis Gambaran Morfologi Limfadenitis Tuberkulosis Menggunakan Metode Biopsi Aspirasi Jarum Halus dan Polymerase Chain Reaction Dedy Suryadi; Delyuzar; Soekimin
Majalah Patologi Indonesia Vol. 29 No. 2, Mei 2020
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v29i2.419

Abstract

BackgroundIndonesia is the 2nd largest of tuberculous (TB) cases in the world. Therefore, TB must be controlled by increasing the capacity anddeveloping universal access needed for TB diagnose accurately and quickly. Progression in controlling TB and decreasing theconsequence can be accelerated through early detection and promt treatment. This study used PCR test as gold standard, albeitthe high cost. Thus, quick, accurate and low cost diagnostic method is needed. In this case morphologic features requirementinclude the existence of epithelioid and necrosis in FNAB smears. Objective was to analyze morphologic features of tuberculosislymphadenitis using FNAB and PCR methods.MethodsThis study is a cross sectional analysis. N samples are all kind of lymphadenitis tuberculosis. Morphologic features in FNABcytology must be confirmed using PCR test and performing chi-square test respectively.ResultsEpitheloid and necrosis features of TB lymphadenitis on FNAB showed 100% positive on PCR methods. This study divided intothree groups. Statistic analyses between group I and II, group II and III showed in significant differences (p=0.074); while betweengroup I and III showed significant differences (p<0.05).ConclusionsPCR method could confirmed morphologic features (epitheloid and necrosis) of TB lymphadenitis

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