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Published by Universitas Negeri Padang

ISSN : 2614669X EISSN : 2579308X DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Bioscience ISSN 2579-308X (Electronic) ISSN: 2614-669X (Print) is peer-reviewed journal and scientific journal publish by Universitas Negeri Padang. The aim of this journal is to publish articles dedicated to all aspects of the latest outstanding developments in the field of biology. Scope of this journal is ;Environmental Biology; Genetics and Biotechnology; Biology of Function; Systematics, Structure and Development.

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Articles 146 Documents

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Histopathological Changes of Testes and Testosterone Level of Mice that are Exposed to Permot Leaf Mosquito Mat (Passiflora foetida) Rina Priastini Susilowati
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

In this study, mice testicular degeneration was influenced by exposure to the mosquito mat made from transfluthrin 3000 ppm and permot leaf mosquito mat which were evaluated based on observations on testicular histopathological changes and testosterone level. Fifteen Balb C male mice aged 2-3 months with a body weight of 25-30 g were divided into five groups, namely A, B, C, D, and E, with each group consisting of 3 replications. Group A was the negative control group (without exposure), group B was the exposure group for the mosquito mat made from transfluthrin 3000 ppm, group C, D, and E were the exposure groups of mosquito mat with permot leaves doses of 1000 ppm, 2000 ppm and 3000 ppm. Each exposure group was given treatment 8 hours per day from 18.00 - 04.00 for 3 months. The results showed a significant decrease in testicular weight between the control group and the treatment group. There were also significant differences in the testosterone level observed in the treatment group, as well as observations on the spermatogenic index (IS). For histopathological observation of testicular mice, there was a reduction in epithelial cell spermatogenic and diameter of seminiferous tubule. The results of the Kruskall Wallis test showed a significant difference between the control group and the treatment group of the mosquito mat made from transflutrin 3000 ppm. The conclusion is that exposure to mosquito mat made of permot peaves up to a 3000 ppm dose is safe to use and can kill the Aedes aegypti mosquito effectively.

Prediction Potential Chlorogenic Acid As Inhibitor Ace (In Silico Study) Yohanes Bare; Dewi Ratih Tirto Sari; Yoga Tribakti Rachmad; Sri Sulistyaningsih Natalia Daeng Tiring; Apriani Herni Rophi; Fitra Arya Dwi Nugraha
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

One of the derivatives of flavonoid explored is chlorogenic acid. Chlorogenic acid has antioxidant and anti-inflammatory activity. Hypertension has support for Angiotensin converting enzyme (ACE) which has a role in regulating the renin-angiotensin system. Hypertension therapy is carried out in inhibit ACE pathway. This study aims to analyze and assess the potential of chlorogenic acid as an anti-hypertensive material by inhibiting the work of ACE. The method used is in silico. Chlorogenic acid ligand was obtained from PubChem while ACE was obtained from RCSB. Interaction of ligand and protein using HEX 8.0.0. Analysis and visualization of the results of interactions using Discovery Study Version 4.1. The results showed an interaction between ligand and protein, namely interactions that occur between chlorogenic acid and sixteen amino acid residues. This interaction produces energy of -292.5cal / mol. This interaction approved the ACE block in the AT-I transformation towards AT-II. Chlorogenic acid has potential as an anti-hypertension material.

TOTAL MICROBIAL PLATES ON BEEF AND BEEF OFFAL Sukmawati Sukmawati
Bioscience Vol 2, No 1 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Efforts to improve people’s nutritional health can be obtained through nutritional food. Beef is a source of animal protein needed. But foodstuffs such as meat and eggs other than as a source of protein is also one of the microorganisms breeding media, which can be a type of human disease. The aim this study was to find out whether the sample of beef, beef heart, and beef liver are safe to consume and marketable. This research method includes tool sterilization, microbial growth medium, and the analysis phase used total plate count method. Samples of beef number of microbial colonies as much as 660 cells/ml. samples of cow’s heart contained colonies 3.150 cells/ml, and in liver samples there are 3.000 cells/ml. so it can be concluded that beef and dairy products are feasible to marketable and to consumption. Keywords: Total Microbial, Beef, Beef Offal

Growth Response Test on Potential Indigenous Bacteria to Degrade Naphthalene and Phenanthrene on various pH Alfi Rizca Hardianti; Helga Lusiana; Dita Widayanti Sawitri
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

This study aims to understand the growth response of indigenous bacteria originating from oil sludge in Dumai which has the potential as Naphthalene and Fenantren degrading agents based on variations in substrate pH. The isolates used in this study were Isolate A, Isolate E, and Isolate F as a result of isolation from oil sludge in Dumai. Based on the results of the growth response test on various concentration in previous studies, we chose E isolates to be tested for its degradation ability. The bacterial growth response data was obtained based on OD measurements every 24 hours using a spectrophotometer and TPC at 72 hours using Nutrient Agar media. Based on these data, we determined the optimum pH for indigenous bacterial growth potential to degrade naphthalene or phenanthrene. In all variations of pH, bacterial isolate E experienced growth. This shows that bacterium E can use naphthalene and fenantren at normal pH range (pH 5-9).

Isolasi dan Identifikasi Bakteri Halofilik Ikan Talang (Chorinemus sp.) dari Aia Bangih Pasaman Barat Mades Fifendy; Faradilla Rattriana; Irdawati Irdawati
Bioscience Vol 1, No 2 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Halophilic bacteria is microorganism that live in high salinity environments up to 30%. The bacteria can be found in foods preserved by salting like salted fish. Salted fish is preserved foods are processed by salting and drying. The bacteria that live and ruin salted fish are halophilic bacteria and heterotoleran bacteria like halobacterium, micrococcus, bacillus, pediococcus, peseudomonas and vibrio. This observation aims to determine type of halophilic bacteria found in talang fish. This is descriptif observation held january up to february 2015 is microbiology laboratory FMIPA UNP and balai veteriner laboratory Bukittinggi. Research methods is cup statterplot methods. Sample is talang fis from Aia Bangih, west pasaman. Based on observation found isolate halophilic bacteria. Its belong to gram positve like coccus. From both, the isolates not capable produce endosopra. Talang fish from Aia Bangih, west pasaman contain two isolate halophilic bacteria consist of a genus and two species.Keywords : talang salted fish, halphilic bacteria, biochemical test

The influence of carbon sources on the production of siderophores from the fluorescent pseudomonad bacteria Ilham Pratama; Linda Advinda; Mades Fifendy
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Abstract.Pseudomonad fluorescent is one of the rhizobacteria groups that could potentially be developed as a crop endurance inducer. Several species of fluorescent pseudomonad are able to produce siderophores. Siderophore is an antimicrobial organic compound that plays a role in biological control of plant diseases. This study aims to determine the best carbon source for the production of siderophores from the fluorescent pseudomonad isolates PfCas3 and PfLAHp2. The carbon sources are fructose, glucose, and glycerol. Detection of siderophores was measured using a spectrophotometer at a wavelength of 410 nm. The results showed that the best growth medium for producing siderophores was KB + glucose medium for both PfCas3 and PfLAHp2 isolates. The best combination was the use of PfCas3 isolate with the addition of carbon glucose source which resulted in the production of siderophores of 1.574. Keywords: Fluorescent pseudomonad, carbon sources, siderophore

Dimensions of Fiber and Jabon Wood Fiber Derivative Value (Anthocephalus cadamba [Roxb] Miq.) in Sialang Dharmasraya and Tabing Padang Regions Adillah Syafitri; Vauzia Vauzia; Des M
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0201931102805-0-00

Jabon (Anthocephalus cadamba [Roxb] Miq.) Is a type of wood that is fast growing and has adaptability in various habitat types. Anatomical characteristics of wood are strongly influenced by environmental factors. This study looked at the dimensions of fiber and the derivative value of jabon wood fiber from the Sialang Dharmasraya and Tabing Padang regions. This research is a descriptive research. Observation of fiber dimensions using wood maceration techniques following the Forest Products Laboratory Method. The results showed that the value of wood fiber dimensions from the Sialang area was higher than those from the Tabing area. In Sialang it has wood fiber length of 603.82-1061.23µm, fiber diameter of 12.37-21.22µm, lumen diameter of 3.81-12.97µm, and wall thickness of 2.75-7.49µm. Whereas, in the Tabing area it has wood fiber length of 592.91-844.38µm, fiber diameter 11.80-20.35µm, lumen diameter 6.14-14.35m, and wall thickness of 1.91-4.54µm. The derivative value of jabon Tabing wood fiber is higher (runkel ratio 0.63-0.62, felting power 41.49-50.25, flexibility ratio 0.70-0.52, coefficient of rigidity 0.22-0.16 and mulsteph ratio 50.27-72.92%) compared to Sialang area (runkel ratio 1.15-1.44, felting power 50.01-48.81, flexibility ratio 0.61-0.30, coefficient of rigidity 0.35-0.22, and mulsteph ratio 62.64 to 90.51%). The quality value of jabon wood fiber at Tabing is better than Sialang. The results of this study can be used as information in jabon wood cultivation.

Community Structure of the Mangrove Forest in the Tourism Area of Pariaman City, West Sumatra Meylia Alvareza; Irma Leilani
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The present study aims to determine mangrove community structure in the mangrove tourism area of Pariaman City. This study has been conducted in October to December 2019 using the purposive sampling method and belt transect in three stations. A total of 21 mangrove species, 20 genera and 19 families was identified in this area. The tree level vegetation of mangroves was dominated by Sonneratia caseolaris (INP = 140,23%). The sapling level vegetation of mangroves was dominated by Dolichandrone spathacea (INP = 174,39%). The seedling and understorey level vegetation of mangroves was dominated by Acanthus ilicifolius (INP = 58%). The diversity of mangrove species in this study area is relatively low to middle (H’ = 1,574 – 2,212).

Isolasi Cendawan Endofit pada Daun Bambu Betung (Dendrocalamus asper Backer.) dan Potensi sebagai Antibakteri Staphylococcus aureus Lydia Yohana Safitri
Bioscience Vol 1, No 1 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Endophytic fungi are microorganisms found in healthy plant tissue but not pathogenic to plants, and produce secondary metabolites same host plant. Secondary metabolites can be used as a drug. The possibility of endophytic fungi are found in bamboo, because bamboo betung (Dendrocalamus asper Backer.) Has high potential as a drug, because it contains flavonoids, and phenolic coumarin. These compounds are secondary metabolites that produce antimicrobial substances that are used in the pharmaceutical field and are found in plant tissue. To avoid excessive use of medicinal plants that could lead to the extinction of these plants, then used the role of endophytic fungi are capable of producing secondary metabolites to the fullest. In addition, the endophytic fungus in plant tissue can be more than one type of fungal endophyte that the higher production of secondary metabolites. This study aims to determine the types of isolates of endophytic fungi on the leaves of Dendrocalamus asper and know the antibacterial potency against bacterial endophyte fungus Staphylococcus aureus. The results showed that there were thirteen isolates of endophytic fungi were isolated from the leaves of Dendrocalamus asper. Ten isolates of endophytic fungi have potential as an antibacterial against S. aureus and three isolates of endophytic fungi did not show any antibacterial activity. Key Word: Endophytic fungi, Secondary metabolites, antibacterial activity.

Correlation of Fasting Blood Glucose With IL-6 Levels in Type-2 Diabetes Mellitus Ethnic Minangkabau Elsa Yuniarti; Syam Syamsurizal; Yuni Ahda; Puja Delfi Sonata
Bioscience Vol 2, No 1 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Diabetes Mellitus (DM) Type 2 is a metabolic disorder disease characterized by an increase in blood sugar (hyperglycemia) due to decreased insulin secretion by pancreatic beta cells or intrusion of insulin function. Hyperglycemia tends to cause oxidative stress where free radical formation exceeds the body's antioxidant defense system resulting in microvascular and macrovascular disorders. Some ethnic groups have a tendency to develop type 2 diabetes mellitus because of differences in diet, lifestyle and physical activity. In addition, ethnicity is also suspected to affect the levels of IL-6. This study aims to determine the correlation of fasting blood sugar levels with IL-6 levels in type 2 diabetes mellitus ethnic Minangkabau. This research is cross sectional comparative research design. The subjects of two groups: DM type 2 and control group (non-DM) who went to the Polyclinic of State University of Padang, Minangkabau ethnic and each consisted of 35 people. Blood glucometer examination with blood sample and IL-6 measurement using ELISA technique with serum sample. Fasting blood sugar levels in patients with Type 2 DM Minangkabau ethnic average 286.2 ± 80.46 mg / dl while non DM 101.26 ± 9.70 mg / dl. Mean IL-6 levels in type 2 DM patients were 16.23 ± 30.12 pg / ml while non-DM of 3.41 ± 1.91 pg / ml was an increase of about 4.7 in patients with type 2 DM Compared to non-ethnic minority of Minangkabau with p value <0,05. The result of research that have been done there is a positive correlation between fasting blood sugar level with IL -6 in patients with Type 2 DM Minangkabau ethnic with r value = 0,44 at p <0.05 which means interpretation of medium correlation strength. Keywords: Type 2 DM, IL-6, ethnic Minangkabau

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