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Published by Universitas Negeri Padang

ISSN : 2614669X EISSN : 2579308X DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Bioscience ISSN 2579-308X (Electronic) ISSN: 2614-669X (Print) is peer-reviewed journal and scientific journal publish by Universitas Negeri Padang. The aim of this journal is to publish articles dedicated to all aspects of the latest outstanding developments in the field of biology. Scope of this journal is ;Environmental Biology; Genetics and Biotechnology; Biology of Function; Systematics, Structure and Development.

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Articles 146 Documents

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Screening of Probiotic Bacteria Candidates in The Mangrove Tourism Area in Klawalu Sorong City West Papua Sukmawati Sukmawati; M Iksan Badaruddin
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Probiotic bacteria was one of the biological control agents (biological control). It had a role in suppressing or killing pathogenic microorganisms. In addition, it also played a role in improving water quality as in fish maintenance media. The basic principle of probiotics was the utilization of the ability of microorganisms to increase absorption in the digestive tract of fish. Probiotic bacteria were able to produce antimicrobial compounds such as lactic acid, hydrogen peroxide, bacteriocin. These compounds were antimicrobial and antibiotic that can suppress the growth of pathogenic bacteria. The purpose of this study was to detect candidate probiotic bacteria from the mangrove tourism area in Klawalu, Sorong City, West Papua. The results of screening for probiotic bacteria candidates by using selective media, from 16 samples consisting of water samples and mud samples’ obtained 11 positive samples, there were probiotic bacteria candidates with the bacterial morphological characteristics of each colony was almost similar. Keywords: Probiotic Bacteria, Mangrove Land, Sorong City

Making Pond And Feed Of Catfish By Farmer’s Wife At Nagari Limau Gadang Armen Amir; Mades Fifendy; Ristiono Ristiono
Bioscience Vol 1, No 2 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/02017128080-0-00

The life of Nagari Limau Gadang residents is poor. They conduct economic activities in Kerinci Seblat National Park (TNKS), so that TNKS become damaged. To reduce the economic activities of the people in TNKS, it is necessary to conduct economic activities outside TNKS to improve the family economy. Catfish farming can serve as the main effort to increase family income. Economically catfish farming is very profitable. Catfish farming is not difficult and can be done by farmers who do not usually cultivate freshwater fish. The right family member is empowered to improve the family economy through the cultivation of catfish is the farmer's wife. Farmer’s wives in Limau Gadang Nagari tend not to improve the family economy, they play the role of taking care of children, doing housework and sometimes delivering food to the fields and to the fields. The farmer’s wife activity is only fulfilling the wife's obligation to husband in married life. Farmers' wives are economically unproductive in improving the family economy. The goal to be achieved in this research is for the wife of farmers can contribute to improve the family economy. Specific targets to be achieved in this study is the wife of skilled farmers cultivate catfish, Nagari Limau Gadang population increases their living standards and TNKS in Nagari Limau Gadang free from the pressure of the population. How to research, selected 20 poor farmer's wife then trained to make ponds and pellets. The pools made are the main pond, spawning pond, nursery pool and pond enlargement. Pellet made from raw trash fish, quail feces, fine bran and cassava. The pellet formulation is made of 4 kinds. Each formulation produces a quality pellet. Based on laboratory test produced pellet contains very good nutrition for enlargement catfish. The nutritional range of protein is 21,79%-34,60%, fat is 1,95%-2,32% and carbohydrate is 26,55%-39,65%. The farmer's wife understands and skillfully creates ponds and pellets. Pellet made from raw materials trash fish, quail feces, fine bran and cassava can be used as raw material pellet. Keyword: cultivation, catfish, farmer's wife, income, family.

Sudut ATD sebagai Penanda Diabetes Mellitus Tipe-2 (DMT2) Syam Syamsurizal
Bioscience Vol 2, No 1 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Dermatoglyphic of patterns (finger prints) could be used as genetic markers with type 2 diabetes. Analysts dermatoglyphic patterns can quantitatively through several parameters: frequency fingerprint patterns (arch, loop and whorl), the number of vines, pattern type index consists of the Dankmeijer index, Furuhata index, pattern intensity index and atd angle. Atd angle is the angle formed by connecting point triradius a, t and d on the palm area. Objective studies link dermatoglyphic characteristics (atd angle) with type 2 diabetes mellitus in Minangkabau ethnic.The research method used descriptive with 132 sample consisted of 66 patients with type-2 diabetes mellitus and 66 control. Results of research on atd angle in type 2 diabetes was 40,44± 3,34 and 40,42±3,96 in controls. The results of t-test and α = 5% p value = 0.75. It can be concluded that there was no significant difference in the average of atd angle in diabetes mellitus type 2 Minangkabau ethnic.

Candida antarctica Lipase B Synthetic Gene: A Bioinformatics Analysis Febriana Dwi Wahyuni; seprianto Seprianto
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Enzyme exploration is important to support the development of biotechnology. To facilitate microbial discovery, it can be done synthetically using bioinformatics methods. Candida antarctica lipase B (CALB) is one of enzyme derived from microorganisms and has been applied as a biocatalyst in several industry. The methods used in this study are the analysis of gene structure using NCBI, analysis of protein CALB sequence using Uniprot, analysis of 3D protein structure using Swiss model, analysis primary design using primer3 and analysis of restriction sites using snapgene. The construction of synthetic calB gene obtained based on the results of the CalB gene sequence in geneBank using NCBI with access number Z30645.1. CalB gene wildtype is modified by adding several restriction enzyme (XhoI, XbaI, ClaI, and BglI), 6 Histags, and 2x stops codon and produce 1083 base pairs. CALB protein has 342 amino acids. Based on 3D structure, CALB protein has three molecules in common with one homotrimer ring that will encircle the double helix DNA. Primers used for calB gene amplification are CALB forward 5'-TCCCCAGTATCAGGTCCAAG-3 ' and CALB reverse 5'-GACACCTGAGGCTGAACGAT-3'.

Carbon Stock Of Seagrass In Karang Tirta’s Coastal Area, Padang Firda Az zahra; Chairul Chairul; Indra Junaidi Zakaria
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The seagrass beds are the spawning, nursery, and the feeding ground for some oceanic biota. It’s also can trapped the sediment and stabilized the substrate so the waters looks clear. Beside those abilities, seagrasses are also can store the carbon. The research was conducted in karang tirta’s coastal area, padang city to analyze the carbon stock of seagrass that stored within their biomass. The biomass was analyzed by harvesting the seagrass at transect 25x25cm, while the carbon stock was analyzed by using walkley & black method. Seagrass beds in karang tirta’s coastal has 14,1 ha (142.546,36 m2) distribution area and it’s stored 18,05 tons of carbon. based on these result the seagrass beds in karang tirta’s coastal area has stored 1,2 tons C/ha. the highest carbon stock has found in the below ground, especially in their rhizomes.

Respon Pertumbuhan Dan Produksi Tanaman Tomat (Solanum Lycopersicum) Dengan Pemberian Bokashi Tithonia (Tithonia Diversifolia) Andina Widya Sari
Bioscience Vol 1, No 1 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Tomato (Solanum lycopersicum) is a vegetable that belongs to the family Solanaceae. Seeing the potential in the domestic and overseas large enough, then the tomato business has a bright prospect. Tomatoes are needed by humans to meet their needs. This is due to the nutritional content of tomato fruit consisting of vitamins and minerals that are very useful for maintaining health and preventing disease, to improve the productivity of tomatoes is done by using fertilizers that Tithonia Bokashi fertilizer. This study aimed to evaluate the response of growth and yield of tomato (Solanum lycopersicum) by giving Bokashi Tithonia (Tithonia diversifolia). This type of research is an experimental study. The study design used was a completely randomized design (CRD) with 6 treatments and 4 replications. The treatments given are dose bokashi Tithonia A = 0 g / polybag, B = Urea, C = 200 g / polybag, D = 400 g / polybag, E = 600 g / polybag, F = 800 g / polybag, parameters tomatoes observed is high, the amount of tomatoes and tomato weight. Data were analyzed using ANOVA and a further test DNMRT at 5% level. Based on the research results show that the tomato plant height, number of tomatoes and tomato fruit weight was significantly different from the provision of Bokashi Tithonia. Dose of 800g / polybag best in improving the response to the growth and production of tomatoes. Key word : Bokashi, Tithonia diversifolia, Solanum lycopersicum

Optimization of Medium Fermentation for Production of Antimicrobial Compounds by Endofit Bacteria Andalas Plant (Morus macroura Miq.) B.J.T.A-6 Isolate Nada Nafion; Dwi Hilda Putri; Irdawati Irdawati
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Cases of bacterial resistance to antibiotics are discussed with serious problems in the world of health. New antimicrobial compounds are needed which are more effective in treating infectious diseases. Isolate B.J.T.A-6 is an endophytic bacteria from Andalas plants (Morus macroura Miq.) which is known to be able to produce antimicrobial active compounds. Antimicrobial compounds can be produced by growing them on fermented media. The purpose of this study was to optimize the medium fermentation of Andalas endophytic bacteria of B.J.T.A-6 isolates in producing antimicrobial compounds. While medium fermentation is Nutrient Broth (NB), Muller Hinton (MH), and Luria Bertani Broth (LB). Antimicrobial activity tests were carried out by means of diffusion. The parameters used were the diameter of the inhibition zone against Staphylococcus aureus around the disc paper. The profile of medium fermentation optimization was analyzed statistically. The results showed that B.J.T.A-6 isolates could inhibit the highest growth of S. aureus by using LB fermentation medium.

Analysis Of ZDS and LCYb Enzyme Coding Gene Related To Beta Carotene Bioshynthesis in Cassava (Manihot esculenta Crantz) using Reverse transcription PCR (RT-PCR) Jihan Rezi Okanti; Dwi Hilda Putri; Ahmad Fathoni
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

This study aims to determine variations in the expression of ZDS and LCYb enzymes encoding several types of yellow tubers. Cassava used, namely Carvita 25, Nangka and Mentega 2 (yellow bulb) and Menti (white bulb) were used as controls. The target enzyme coding genes (ZDS and LCYb) in cassava were identified in an online database, Phytozome using the BLAST method. Design and primary analysis for the target genes was carried out using online software, OligoAnalyzer and PCR. The expression of the target enzyme coding gene was analyzed using the Reverse Transcription PCR (RT-PCR) method. The BLAST results in the cassava genome in the Phytozome database showed that the enzymes ZDS and LCYb in cassava were encoded by two genes. The primary optimization results showed the primary annealing temperature for the LCYb enzyme coding gene was 55 ° C. The ZDS primers were not amplified after several replications, so that only the LCYb enzyme coding gene was selected for qualitative gene expression analysis using RT-PCR. A positive control in the analysis of gene expression is the housekeeping gene, PP2A. Electrophoresis results of PCR products (RT-PCR) showed negative results (no DNA bands were detected) in all samples from both yellow and white tuber samples and its housekeeping gene. This is possible from the low quality of RNA used in cDNA synthesis.

Isolation of Phosphate Solubilizing Endophytic Fungi From Rice Plant Root Dezi Handayani; Mades Fifendy; Verawati Yesni
Bioscience Vol 2, No 1 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Root endophytic fungi plays different roles for plant, such as plant growth promoting properties, agents to control phytopathogens, and increase phosphorus uptake. Since phosphorus are essential for plant growth and its occurance are limited, so it is necessary to explore these fungus to replace the used of synthetic fertilizer. The objective of this study were to obtain root endophytic fungi from rice plant and to determine its phosphate solubilization ability. The root organ of rice plant was subjected for isolation. Pikovskaya medium was use to determine the fungal phosphorus solubilization ability. Fungal morphological characteristics was carried out by macroscopic and microscopic appearance assessment using microscope. Seven endophytic fungi were successfully isolated from rice plant root sample. Four isolate were micelial steril with no conidia, two isolate refers to Aspergillus and 1 isolate have 2-4 conidia at the tip of conidiophores. Amongs 7 endophytics fungi, only one isolate (P2B3) had the ability to solubilize phosphate with the phosphate solubilization index value 20.45 %.

Diversity Of Nocturnal Insects (Insecta) In Bukik Kasang, Padang Pariaman, West Sumatra Emelia Debora; Rijal Satria
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The study of nocturnal insect diversity was conducted in Bukik Kasang, Padang Pariaman District, West Sumatra from April to May 2019. The pitfall trap was used to collect the active insect on the ground in three different habitats: open area, edge of plantation, and inside the plantation. A total 10 orders, 44 morphospecies, and 180 individual of nocturnal insect was collected. The Order Hymenoptera was the highest in number of morphospecies and individual, and in other hand, the Order Hemiptera was the lowest one. This study is very important to reveal the diversity of insect in Bukik Kasang, due to the increasing of human activities in this area and planning to develope this area as tourist destination.

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