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146 Documents
<![CDATA[Avifauna Potential for the Development of Bird Watching Ecotourism on Mount Tandikek ]]>
<![CDATA[Rudi Yuliandri]]>;
<![CDATA[Ramadhan Sumarmin]]>
<![CDATA[ Bioscience Vol 4, No 1 (2020): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/0202041105493-0-00
<![CDATA[One of the economic benefits of birds is the development of birdwatching ecotourism. Birdwatching ecotourism can make recreation more effective and beneficial. Mount Tandikek with a high level of biodiversity is one area that has the potential to become a birdwathing ecotourism location. Based on this background, this research is important to do. this was done in June 2019 until July 2019 using the Point Count method combined with the Visual Encounter Survey (VES) method. Observation starts in the morning until late afternoon by following the hiking trail via Singgalang Gantiang from the bottom up. The data obtained were processed descriptively, and the diversity index and evenness index were calculated. The results obtained as many as 136 individuals from 35 species of birds during observation. There are 5 species of birds protected by PP. No. 7 of 1999, among them: Spilornis cheela, Spizaetus alboniger, Falco peregrinus, Todiramphus chloris and Nectarinia jugularis. The bird diversity index on Mount Tandikek is high with H '= 3.084. The average category of the binary evenness index is 0.628. The species of birds that dominated the observation were Phylloscopus trivirgatus for 16 individuals, Ficedula westermanni for 15 individuals and Pericrocotus miniatus for 14 individuals. Bird species found on Mount Tandikek have the potential to become birdwatching ecotourism objects.]]>
<![CDATA[Pengaruh Kombinasi Pseudomonad Flouresen Dan Em4 Dalam Menghambat Pertumbuhan Blood Disease Bacteria (Bdb) Penyebab Penyakit Darah Tanaman Pisang Secara In Vitro ]]>
<![CDATA[Maemunah Maemunah]]>
<![CDATA[ Bioscience Vol 1, No 1 (2017): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/02017117436-0-00
<![CDATA[Blood Disease Bacteria is one obstacle in cultivation of bananas. BDB can be controlled using biological agents. Biological agents that can be used to control of blood diseases are Pseudomonad fluorescent and microorganisms in EM4. Pseudomonad fluorescent used as biological agents because of its ability to produce antimicrobial compounds such as siderophores, antibiotics, volatile and cyanide. other than that, the EM4 microorganisms can produce antimicrobial against several bacterial pathogens. This study aims to determine the effect of combination Pseudomonad fluorescent and EM4 to inhibit the growth of BDB in vitro. This research was conducted in January 2016 in the Laboratory of Microbiology Department of Biology FMIPA UNP. Research using a completely randomized design (RAL) with 6 treatments and 3 replications. The study treatment is a combination of Pseudomonads fluorescent and EM4. The observation was done by measuring the clear zone formed around the paper disc. Data were analyzed using analysis of variance (ANOVA) and the test of Duncan New Multiple Range Test (DNMRT) at 5% level. The research proves that the combination resulted in inhibition zone around the paper disc. Inhibition zone shows that the combination inhibits the growth of BDB in vitro. Inhibition zone is formed as fluorescent Pseudomonads and microbes on EM4 produce secondary metabolites that can inhibit the growth of BDB.Key Word: Pseudomonad flourecent, BDB, EM4]]>
<![CDATA[Effect of Boiled Water Tithonia diversifolia A. Gray Leaf Against the Pancreas Histology in Mus musculus L. Induced by Alloxan ]]>
<![CDATA[Yenni Fitri]]>;
<![CDATA[Elsa Yuniarti]]>
<![CDATA[ Bioscience Vol 3, No 1 (2019): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/0201931103432-0-00
<![CDATA[Traditional medicine is one of the drugs used by the community to be one of the efforts to treat diseases. One of them is Diabetes Mellitus (DM). DM is a metabolic disease characterized by hyperglycemia which results in an increase in free radicals in the cell. DM treatment is quite expensive so an alternative drug is needed. One of them is Tithonia diversifolia A. Gray. This study used a completely randomized design, consisting of 1 control, 4 treatments (P1: Only alloxan induced), P2: Alloxan 65 mg / kg BB, P3: Metformin 65 mg / kg BB, P4: Boiled leaves of moon leaves 24.6 mg / 10 ml and P4: Moon flower leaves boiled water 49.1 mg / 10 ml. The parameters observed were blood sugar in male mice and the number of cells in the islands of Langerhans before and after being given boiled leaves of moon flowers and metformin. Data were analyzed using ANOVA then continued with DMRT test with a significant difference of 5%. The results showed that administration of boiled kembang bulan leaves and metformin for 7 days could reduce blood sugar levels in mice and accelerate the regeneration of pancreatic cells. The most significant impact is the treatment with the highest dose, namely P4 (Moon leaf leaves boiled water 49.1 mg / 10 ml).]]>
<![CDATA[Analysis of Genetic Profiles of Heavy Metal Phytoremediator Plants From Gold Mining Areas ]]>
<![CDATA[Sih Winarti]]>;
<![CDATA[Liswara Neneng]]>;
<![CDATA[Yohanes Edy Gunawan]]>;
<![CDATA[Chaidir Adam]]>
<![CDATA[ Bioscience Vol 4, No 1 (2020): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/0202041108305-0-00
<![CDATA[The resistance and survival ability of phytoremediator plants in a polluted environment is thought to be a form of genetic adaptation. This research aimed: (1) to identify the dominant plant species in ex-gold mining area; (2) to analyze the genetic profile of phytoremediator plants from ex-gold mining area; and (3) to compare the genetic profile of heavy metal phytoremediator plants from ex-gold mining area with the same plant species from non-mining area. The samples of this study were Cyperus sp., Lycopodium sp., and Melastoma sp. The research procedures carried out include sample collection, DNA isolation, DNA amplification with PCR, and DNA visualization with Electrophoresis. The results show that the dominant plant species of ex-gold mining area are Cyperus sp., Lycopodium sp., and Melastoma sp. The genetic profile analysis of dominant plant species of ex-gold mining area show that no DNA bands appeared from the target gene as the result of amplification using specific primers of Metallothionein gene. The result of RAPD analysis using OPA-04 universal primers show that at 500-750 bp there are differences in DNA bands that appeared between the samples. DNA bands that appeared in the genetic profile of phytoremediator plants is thought to be the representation of the gene that responsible for heavy metals tolerance.]]>
<![CDATA[Sudut ATD sebagai Penanda Diabetes Mellitus Tipe-2 (DMT2) ]]>
<![CDATA[Syamsurizal Syamsurizal]]>
<![CDATA[ Bioscience Vol 1, No 1 (2017): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/02017117162-0-00
<![CDATA[Pola dermatoglifi (sidik jari) dapat digunakan sebagai penanda genetik DMT2. Analis pola dermatoglifi secara kuantitatif dapat melalui beberapa parameter dermatoglifi antara lain: frekuensi pola sidik jari (arch, loop dan whorl), jumlah sulur, indeks tipe pola (pattern type index) yang terdiri dari indeks Dankmeijer, indeks Furuhata, indeks intensitas pola (pattern intensity index) dan Sudut atd. Sudut atd merupakan sudut yang terbentuk dengan menghubungkan titik triradius a, t dan d pada daerah telapak tangan.Tujuan penelitian menemukan hubungan karakteristik dermatoglifi (sudut atd) dengan diabetes melitus tipe-2 etnis Minangkabau.Metode penelitian yang digunakan deskriptif dengan 132 sampel terdiri dari 66 pasien dengan diabetes melitus tipe-2 dan 66 kontrol. Hasil penelitian tentang sudut atd pada DMT2 adalah 40,44± 3,34 dan pada kontrol 40,42±3,96. Hasil uji-t dan α=5% didapatkan nilai p=0,75. Dengan demikian dapat disimpulkan bahwa tidak ada perbedaan yang signifikan rata-rata sudut atd pada diabetes melitus tipe-2 etnis Minangkabau.Kata kunci: sudut atd, dermataglifi dan DMT2]]>
<![CDATA[Optimasi Sistem Transformasi Gen Xiloglukanase Pada Eucalyptus pellita F. Muell Menggunakan Agrobacterium tumefaciens ]]>
<![CDATA[Sri Hartati]]>;
<![CDATA[Andriyani Puspitaningrum]]>;
<![CDATA[Nita Etikawati]]>;
<![CDATA[Enny Sudarmonowati]]>
<![CDATA[ Bioscience Vol 2, No 1 (2018): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/020182110044-0-00
<![CDATA[Eucalyptus pellita is a type of woody plant that is widely used as raw materials of pulp and paper so that the need for wood from this type of plant is increasing. Improvements in wood quality such as cellulose deposition and increased growth rates are needed to support the supply of raw materials for the pulp and paper industry. One technology to change the composition of wood is the modification of plant cell walls through the transformation of xylolukanase gene which in other plants such as Populus alba and Acacia mangium have been shown to increase cellulose deposition and spur growth. The purpose of this study was to obtain an efficient xyloglucanase transformation method in E. pellita using Agrobacterium tumefaciens. Sprouts E. pellita 006 and 06A with different ages of 8 and 15 days are used as plant material for transformation. The sonication treatment of the sprouts prior to the transformation was also applied to determine the effect on transformation efficiency. Transformation is done by soaking the seeds that have been through the treatment of sonication and without sonication on the suspension of Agrobacterium carrying plasmid pAa XEG300 and subsequently grown on the selection medium. Sprouts E. pellita 006 aged 15 days without sonication treatment showed the highest percentage of regeneration in the selection media that is equal to 72%. Gene integration testing through DNA amplification with specific primers showed a ribbon of xyloglucanase with a size of 709 bp.]]>
<![CDATA[Kombucha Tea Production Using Different Tea Raw Materials ]]>
<![CDATA[Putri Ardila Sari]]>;
<![CDATA[Irdawati Irdawati]]>
<![CDATA[ Bioscience Vol 3, No 2 (2019): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/0201932105584-0-00
<![CDATA[Tea (Camellia sinensis) is an agricultural product that is widely issued by people around the world. Some types of tea in the world are green tea, black tea, oolong tea and white tea. Kombucha tea is brewed water for tea and sugar then given bacteria and fungi and fermented for 7-10 days. The purpose of this study was to determine the quality of kombucha of several different types and determine the organoleptic value of fermented kombucha tea using different types. This research was conducted in April-July 2019 in the Microbiology Laboratory of the Department of Biology FMIPA Padang State University. This research is an experimental study with 5 treatments and 1 repetition (K: kombucha starter, P1: kombucha starter + green tea, P2: kombucha starter + black tea, P3: kombucha starter + oolong tea, P4: kombucha starter + white tea. Parameters Calculated are the number of microbes, nata thickness, pH of kombucha tea, total acetic acid, and organoleptic tests of kombucha tea.The results of this study indicate differences in the types of tea used in kombucha fermentation, the quality of kombucha tea produced, kombucha tea using black tea. The organoleptic test requested by the panelists was kombucha tea from white tea.]]>
<![CDATA[The Extract of Tristania Sumatrana Miq. to Lower Primary, Secondary and Tertiary Follicles in Swiss Webster Mice ]]>
<![CDATA[Syamsurizal Syamsurizal]]>;
<![CDATA[Arief Muttaqiin]]>
<![CDATA[ Bioscience Vol 1, No 2 (2017): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/02017128063-0-00
<![CDATA[The use of traditional medicinal plants is generally based on empirical experience; therefore, a scientific approach is needed to bring traditional medicine into the practice of medicine and formal health services. Tristania sumatrana Miq. is one of the traditional medicinal plants that are often used as contraceptives for women in the villages of Lembah Bawan and Kampung Dalam (West Sumatra). The extract of Tristania sumatrana Miq. can extend the estrus cycle of Mus musculus into eleven days. The purpose of this research is to know the effect of Tristania sumatrana Miq. extract to the fertility of female mice. The experiments were conducted with a randomized 5x2 group design. Five dosage groups: untreated control, placebo, treatment with doses 600, 900 and 1200 mg/kgbw and two treatment groups: 10 and 20 days. The fertility parameters studied were ovarian weight, number of primary, secondary, tertiary follicles. The results prove the extract of Tristania sumatrana Miq. causing a very significant decrease in ovarian weight (treatment of 900 mg/kg bb for 10 days), number of primary and secondary follicles (1200 mg / kg bb for 10 days), and tertiary (600 mg / kg bb for 20 days). The extract of Tristania sumatrana Miq. causing a decline in Mus musculus fertility.]]>
<![CDATA[The influence of carbon sources on the production of siderophores from the fluorescent pseudomonad bacteria ]]>
<![CDATA[Ansel Nastika]]>;
<![CDATA[V Violita]]>;
<![CDATA[Irma Laelani Eka Putri]]>
<![CDATA[ Bioscience Vol 2, No 2 (2018): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/0201822101113-0-00
<![CDATA[Land kangkung (Ipomoea reptans Poir.) is one of the types of kangkung plants that are quite easy to be cultivated but because of the narrowness of urban agriculture land, it is difficult for the people to cultivate kangkung. Now there are one alternatives to exploit narrow land as an effort to develop organic farming, that is by farming in a hydroponic way. This study aims to determine the effect of liquid organic fertilizers made from Sargassum sp. on the growth of land kangkung (Ipomoea reptans Poir.) with hydroponic technique. This research is an experimental research conducted in December 2017 until April 2018, housed in research laboratory and home wire Biology FMIPA UNP. This study used Completely Randomized Design (RAL) 6 treatments and 4 replications. The main observation parameters include Plant height (cm), Number of Leaves, Leaf Area (cm2), Plant Wet Weight (g) and Dry Weight Plant (g). The data were analyzed using Analysis of Variance (ANOVA) test and DNMRT further test at 5% level. The results showed that liquid organic fertilizer made from Sargassum sp. had no signifficant effect on plant height, surface area, number of leaves, wet weight and dry weight of land kangkung (Ipomoea reptans Poir.)]]>
<![CDATA[The Influence of Bioinsektiidide Variation of Tuba Root Extract (Derris elliptica Roxb. Benth) On Phantsahm Mortality the Pest (Leptocorisa Acuta Thumberg) ]]>
<![CDATA[I Gede Warse]]>;
<![CDATA[Handoko Santoso]]>;
<![CDATA[Rasuane Noor]]>
<![CDATA[ Bioscience Vol 3, No 1 (2019): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/0201931103757-0-00
<![CDATA[Leptocorisa acuta Thumberg is one of the pests that many rice farmers complain about. This pest can cause damage to rice plants while still young, so the results obtained are not optimal. Based on these problems it is necessary to use insecticides so that the pest can be controlled. Derris elliptica Roxb. Benth is one of the many plants that grow in Indonesia. The root of this plant has a chemical compound in the form of rotenone which has the potential as a bioinsecticide to eradicate pest parasites. The study was an experimental study using a completely randomized design method with three treatments and one control with 6 replications. The treatment dose in this study using root extract Derris elliptica Roxb. Benth. Bent: 8%, 10%, 12% and 0% control. Based on the Kruskal Wallis Test the results were x2calculate 10.88 > x2table 9.49 at a 0.05 in the Chi-square table, so that there was an effect of the variation of the bioinsecticide dose of root extract Derris elliptica Roxb. Benth the impact on mortality of the Imago Leptocorisa acuta Thumberg. Based on the average ranking of treatment at a dose of 12%: 6.7, a dose of 10%: 8.46, a dose of 12%: 8.76 and a control of 0%: 18, so it can be concluded that root extract Derris elliptica Roxb. Benth the most effective against the mortality of imago Leptocorisa acuta Thumberg was at a dose of 12%.]]>
