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Published by Universitas Negeri Padang

ISSN : 2614669X EISSN : 2579308X DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Bioscience ISSN 2579-308X (Electronic) ISSN: 2614-669X (Print) is peer-reviewed journal and scientific journal publish by Universitas Negeri Padang. The aim of this journal is to publish articles dedicated to all aspects of the latest outstanding developments in the field of biology. Scope of this journal is ;Environmental Biology; Genetics and Biotechnology; Biology of Function; Systematics, Structure and Development.

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Articles 146 Documents

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Respon Pertumbuhan Benih Padi Varietas Anak Daro Asal Solok Terhadap Isolat Trichoderma Indeginous Azwir Anhar; Dwi HIlda Putri; Febri Doni; Linda Advinda
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Trichoderma spp adalah jamur non patogen yang berasosiasi dengan rhizosfer dan daun tanaman. Jamur ini juga mampu mengendalikan penyakit dan menghasilkan zat pengatur tumbuh, sehingga potensial digunakan dalam pertaniankhususnya dalam budidaya pertanian organik. Penelitian kemampuan Trichoderma spp dalam meningkatkan pertumbuhan benih padi masih terbatas. Penelitian ini, melaporkan kemampuan Trichoderma setempat untuk meningkatkan poertumbuhan kecambah benih padi. Hasil penelitian menunjukkan bahwa isolat Trichoderma mampu meningkatkan daya kecambah dan vigor benih padi. Hasil penelitian membuktikan bahwa isolat Trichoderma indegenous memiliki potensi untuk digunakan sebagai pengatur pertumbuhan benih padi. Hasil penelitian meningkatkan pemahaman lebih lanjut tentang peranan jamur dalam meningkatkan pertumbuhan padi

Pengaruh Insektisida Organoklorin Endosulfan Terhadap Daya Tetas Telur Ikan Nila (Oreochromis Niloticus) Anggia Cahyati Putri
Bioscience Vol 1, No 1 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Uncontrolled usage of pesticides can contaminate the environment such as water, soil, and air. This occurs because the residue of pesticides. One of them, organochlorine insecticides have widely used by farmers because it is persistent. This study aims to look at the effects caused by organochlorine insecticide endosulfan on hatchability of eggs tilapia (Oreochromis niloticus). This research was conducted CRD with 6 treatments and 4 replications. Concentrations of organochlorine endosulfan in treatment are determined based on the LC50. The treatment use organochlorine endosulfan at concentrations of 0.00395%, 0.00400%, 0.00405%, 0.00410% and 0.00415%. Then they observed for 4 days (96 hours). The data obtained and analyzed by ANOVA and followed by DNMRT (Duncan's New Multiple Range Test). The result showed organochlorine insecticide endosulfan significantly (p<0,05) affect hatchability of eggs tilapia (Oreochromis niloticus), but no significant effect on larval abnormalities. Hatchability of eggs tilapia (Oreochromis niloticus) and the highest larval abnormalities are found in P1 which is a control treatment. While the hatchability of eggs and larvae abnormalities lowest for the P6 treatment with concentrations of endosulfan treatment 0.00415%.Keywords: endosulfan, Tilapia (Oreochromis niloticus), hatchability

Isolation and Identification of Endophytic Bacteria from the Andalas Plant Stem (Morus macroura Miq.) Nisa Afifah; Dwi Hilda Putri; Irdawati Irdawati
Bioscience Vol 2, No 1 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The number of death couse of infection continues to increase. Treatment of infections is increasingly difficult due to increased resistance of germs to existing antimicrobial compounds. A new source of antimicrobial substances is needed. One source of antimicrobial substances is endophytic bacteria that live in the stems of Andalas plants. The aim of this study was to compare the diversity of endophytic bacteria present in old and young Andalas plant stems. The method used to isolate endophytic bacteria from the stem of the Andalas plant is the streak plate method. Bacterial identification is done macroscopically and microscopically. The results of macroscopic observation showed that there are differences in the number of isolates found in old and young Andalas plant stems. 11 isolates of endophytic bacteria from Andalas plant stems, each of which consists of 9 endophytic bacteria from old Andalas stems and two isolates from young Andalas stems. A microscopic identification was found that of the 11 isolates isolated, eight isolates were gram-positive and three isolates were gram-negative bacteria

Effect of Time Incubation To Quality and Organoleptics Soygurt Mades Fifendy; Syukryani Syukryani
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Abstract. Soy is a soursce of vegetable protein that is recommended for consumption. One of the soybean ingredients known to the general public is soy milk. Soy milk is a health drink that has nutritional value equivalent to cow's milk. Utilization of soy milk is still limited because the flavor is less favored. To get a good taste can be processed through the process of fermentation by utilizing the bacteria Streptococcus thermophillus and Lactobacillus bulgaricus which will produce Soygurt. How long is the proper incubation time to produce soygurt at room temperature is unknown. The purpose of this research is to know the effect of incubation time on quality and organoleptic value of soygurt. This research is an experimental research with Completely Random Design 8 treatment, 3 replications. The treatments consisted of different incubation periods: 0 hours (control), 10 hours, 12.5 hours, 15 hours, 17.5 hours, 20 hours, 22.5 hours, 25 hours. Parameters measured were acidity, fat content, protein content and organoleptic value. The acidity data was analyzed by Anava. The results showed that the treatment given significant effect on acidity level. Levels of acidity, fat content, and protein content meet the Indonesian National Standard. For oerganoleptic test the highest score for color was on the 17,5 hour treatment and the viscosity was at 22,5 hours and 25 hours treatment. While for the favorite test is the treatment of 10 hours, 12.5 hours, 17.5 hours and 20 hours. The incubation time to affects the quality and value of the organoleptic soygurt.

Ethnobotani Study on Mangrove Forests in Padang City and Pariaman City Irma - Leilani; Rizki Rizki; Tuti M Sari; Dian M. Sari
Bioscience Vol 1, No 2 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

There are many benefit especially for the society who live around mangrove area. The purpose of this study is to find out the species of mangrove that used by the society in Teluk Buo Padang and in Mangguang, Pariaman. This research was conducted on Mei 2012 till June 2013. This study used survey method with direct observation to the field. interviews conducted to determine the utilization of mangrove species for the surrounding community. There were 43 people in Pariaman and 33 people in Padang have been selected as the respondent. The interview aims to find out the species of mangroves that are used as medicinal plants, for foods or other benefits and how to used it. In Pariaman we found 19 species, 10 species are medicinal plants, 3 can be eaten and 18 species are used for other things. In Padang we found 17 species, 11 of them are medicinal plants, 3 can be eaten, 15 species are used for other things. Some species have many benefits by people around the mangrove forest Key words: Benefit, mangrove, Pariaman, Padang

Exploration of Family Rutaceae in Garden Eden 100 Tobasa Agrowisata Forest Nurhasnah Manurung; Pandu Prabowo Warsodirejo
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Research in the Eden 100 agrotourism forest located in the area of Lumban Rang, Sionggang Utara Village, LumbanJulu District, Kab. Toba Samosir North Sumatra has been carried out for 3 months using "Survey or Exploration Method". The aim of this research is to find out data on plant species of family Rutaceae in the Eden Park area 100 and then make herbs and to produce learning devices in the form of dabbing as ingredients teach Biology courses in High Plant Taxonomy. The results showed that there were 9 types of plant family Rutaceae with the Genus Citrus as many as (6), Genus Zanthoxylum (1), Genus Triphasia (1), Genus Aegle (1), so the most dominant is the Genus Citrus. such as Lime (Citrus aurantifolia), Andaliman (Zanthoxylumachanthopodium), Orange Purut (Citrus hystrix DC), Tiger Orange (Citrus medica), Kingtit Orange (Triphasiatrifolia), Lemon Orange (Citrus lemon), Kasturi Orange (Citrus madurensis) , Sweet / Squeezed Oranges (Citrus sinensis), AegleMajamarmelos (L.) Corr. The Rutaceae family plant found in the Eden 100 agrotourism forest is still relatively small when compared with the total total genus Rutaceae 150 genera and 1500 species

The Effect of Coffee Arabica (Coffea arabica L.) Fruit Skin Extracts on Small Intestine Morphometry of mice (Mus musculus L.) with Ethanol-Induced Rita Maliza; Febriofca Galih Yatalaththov; Haris Setiawan; Listiatie Budi Utami
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151111571-0-00

Consumption of alcoholic beverages can increase ROS and inflammation. In the duodenal, the consumption of alcohol can result in erosions, bleeding, and mucosal injury. Arabica coffee fruit skin contains antioxidant substances, which are expected to have a good role as an antioxidant effect. Currently, unknown effects of alcohol and extract of Arabica coffee fruit skin administration on the damage of the small intestine of mice (Mus musculus L.). This study used an experimental design, and 25 males of mice were divided into five groups of treatment for 15 days. After the treatment, the mice at necropsied and the small intestine were taken for histopathological examination using hematoxyline-eosin staining. The Data analyzed used One Way ANOVA and Duncan's tests. Histopathological observations showed a dose of 250 mg/kg BW experienced the most significant improvement damage of the small intestine of mice compared to other treatments. Histomorphometry measurement, The dose of 250 mg/kg BB showed the high of villi 272,10 ± 13,36 µm, (P<0.05), duodenal villi crypt depth 117,33 ± 30,35 µm (P<0.05) and surface area of small intestinal villi 7,66 ± 0,55 mm2 (P<0.05) was similar with negative control. The conclusion of this study is a dose of 250 mg/kg BW treatment was able to improve the histopathology and morphometry small intestine of mice that were damaged due to consumption of ethanol 15%.

Artificial Feed from Organic Waste for Carp (Cyprinus carpio L.) Wolly Candramila; Muhammad Taufik Riandi; Dani Abdillah; Eko Widodo
Bioscience Vol 4, No 2 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202042109311-0-00

Carp (Cyprinus carpio L.) is one of the widely cultivated fishes in Indonesia. Utilization of organic waste from the traditional market such as chicken intestines, corn husk, and water spinach stems into artificial feed for carp (Cyprinus carpio L.) var. Majalaya is discussed in this study. Compared to the commercial feed commonly used by fish farmers in Pontianak, artificial feed with a protein content of 30% showed the best growth rate of body length and weight, and highest feed efficiency. With the proper cultivating condition, artificial feed presents a huge opportunity to be an alternative solution to reduce the cost of carp farm production.

Analysis of Genetic Variations in Poly Gene Sequences in Dengue Virus 2 Using In-Silico RFLP Afifatul Achyar; Alvenaya Hindayageni; Fadhila Humaira; Nurfadillatun Nisa Wijaya; Nur Aqsha; Zultsatunni’mah Zultsatunni’mah
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151111916-0-00

Dengue hemorrhagic fever (DHF) is a disease caused by Dengue virus transmitted to humans through infected Aedes aegypti and Ae. Albopictus. Dengue virus classified into 4 serotypes, including DEN-1, DEN-2, DEN-3, and DEN-4. The genomes of the four dengue viruses share about 65% similarities, the rest is a genetic variation that differentiates between serotypes. Genetic variations also occur even within one serotype. This study aimed to analyse genetic variation in envelope protein E (POLY) gene sequences in Dengue Virus 2 NCBI PopSet 1760494694 using in-silico RFLP by free bioinformatic tools on internet. The restriction enzymes used were AfeI and ApaLI. The results of the in-silico RFLP in this study showed that there were genetic variations in the recognition site of the AfeI enzymes (A1 allele and A2 allele) and ApaLI (B1 allele and B2 allele) in 18 DNA sequences of the POLY gene for Dengue 2 virus NCBI PopSet 1760494694.

Antibacterial Activities of Kombucha Tea From Some Types of Variations of Tea on Escherichia coli and Staphylococcus aureus Witri Nofita Safitri; Irdawati Irdawati
Bioscience Vol 4, No 2 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202042105679-0-00

Tea can be used as a fermented drink called kombucha tea. Based on the oxidation process tea is classified into white tea, green tea, oolong tea and black tea. The compounds produced from the fermentation of kombucha tea have a fairly good antibacterial effect. This study aims to determine the antibacterial activity of kombucha tea fermentation from several variations of tea types against Escherichia coli and Staphylococcus aureus. This research is an experimental study with a completely randomized design (CRD) with 5 treatments and 3 replications conducted in May-June 2019 at the Microbiology Laboratory and the Integrated Research Laboratory of the Department of Biology FMIPA UNP. Kombucha tea used in this study was white tea, green tea, oolong tea and black tea. Antibacterial activity test was carried out by paper disc diffusion method against microbial test of S. aureus and E. coli bacteria. Research results showed that the highest antibacterial activity of kombucha tea fermentation in E.coli was found in black tea with a value of 1.64 cm and the lowest in tea white with a value of 1.04 cm. In S. aureus the highest value was obtained in black tea with a value of 2.48 cm and the lowest in white tea with a value of 1.39 cm.

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Syamsurizal

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syam_unp@fmipa.unp.ac.id

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Sumatera barat

INDONESIA