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Bioscience
Published by Universitas Negeri Padang
ISSN : 2614669X     EISSN : 2579308X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Bioscience ISSN 2579-308X (Electronic) ISSN: 2614-669X (Print) is peer-reviewed journal and scientific journal publish by Universitas Negeri Padang. The aim of this journal is to publish articles dedicated to all aspects of the latest outstanding developments in the field of biology. Scope of this journal is ;Environmental Biology; Genetics and Biotechnology; Biology of Function; Systematics, Structure and Development.
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Articles 146 Documents
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The Sub Acute Toxicity Study of Purified Gambir (Uncaria gambir Roxb.) to Liver Histology and its Reversibility on Rats Dita Permatasari; Indri Oktavia; Armenia Nazar; Almahdy Ahmadin
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151111642-0-00

Abstract

Purified gambir proved to have many pharmacological activities and has the potential to be developed into standardized herbal medicines. This study was conducted to determine the safety of purified gambir to the liver histology. A total of 30 female white rats were used which divided into 4 groups: one control group and 3 test groups treated with purified gambir at doses of 5, 10 and 20 mg/kgbw orally for 14 days respectively. On the scheduled days, three animals from each group were killed for liver histological examination. The degree of liver damage and liver organ ratio in this study where were measured. The reversibility of liver damage was observed from the change in the value of the damage degree and liver organ ratio after the administration of gambir was stopped. Data were analyzed using two-way ANOVA followed by Duncan (significance were taken at p<0.05). The administration of purified gambir in rats for 14 days at tested groups caused significant damage to liver tissue (p<0.05). In this situation, the degree of tissue damage increased and the liver organ ratio decreased in all tested groups. After the administration was stopped, the degree of liver damage is decreased insignificantly, meanwhile the liver organ ratio is significantly increased.
Antibacterial Activity of Coffee Arabica (Coffea arabica L.) Fruit Skin Methanol Extract On Bacteri Eschericia coli and Staphylococcus aureus Rita Maliza; Jannati Aulah; Oktira Roka Aji
Bioscience Vol 4, No 2 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202042108692-0-00

Abstract

Coffee fruit skin is a waste from coffee bean processing. Arabica coffee bean extract (Coffea arabica L.) is known to contain anthocyanin compounds, polyphenols, beta-carotene, and vitamin C which have the potential as antimicrobial agents. The purpose of this study is identification and anaysis the optimal concentration of Arabica coffee peel extract which inhibits the bacteria Escherichia coli and Staphylococcus aureus. This study used an experimental method consisting of 3 extract concentration treatments, positive control, and negative control with 3 repetitions. The treatments tested were 25%, 50%, 75% coffee fruit peel, negative control (sterile distilled water) and positive control (Ampicillin 2 µg / ml). The bacterial test was carried out using the in vitro method. The parameters measured were the amount of inhibition zone (mm) formed on the Mannitol Salt Agar (MSA) and Mac Conkey media. Analysis of research data using the One-way ANOVA test and Duncan test at α = 0.05. The results showed that the optimum concentration of the extract against Staphylococcus aureus was 50%, while the optimum concentration of Escherichia coli was 25%. The results of this study indicate that the extract can be an antibacterial that can inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria optimally.  
Study of Forest Types, Inventory of Tree, and Chlorofil Contents of Malabar Forest Leaves, Malang City Roimil Latifa; Endrik Nurrohman; Samsun hadi
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151111466-0-00

Abstract

This research aims to examine the forest types, inventory of tree species, and chlorophyll content of plant leaves in the Malabar forest, Malang City. This type of research is descriptive quantitative. This research was done from the month of August to December 2020 and took place in Malabar Forest and Biology Laboratory of University of Muhammadiyah Malang. Data collection methods using exploratory techniques and laboratory observations. Data analysis is done by descriptive quantitative. The results showed that 101 tree species inhabit Malabar forest with the most Tanjung trees with 175, and the least number is Srikaya with only one tree. Malabar forest is classified as a forest with a spreading form. In the dry season, the average chlorophyll ‘a’ content of leaves was highest in starfruit leaves (35.848 µg/ml), the lowest average of starfruit leaves (17.857µg/ml), the average chlorophyll ‘b’ content of leaves was highest in Tabebuya leaves (58.862µg/ml). The lowest was Norfolk Pine leaf (9,124 µg/ml), the highest total leaf chlorophyll was Tabebuya leaf (91,737µg/ml), and the lowest was the Norfolk Pine leaf (28,517µg/ml). In the rainy season, the highest chlorophyll ‘a’ content was Sengon tree (34.3µg/ml) and the lowest was Chocolate (0.3µg/ml), the highest chlorophyll ‘b’ was Genitu (131.6µg/ml) the lowest was Lamtoro (6.5µg/ml), the highest total chlorophyll was Melinjo (90.7µg/ml) and the lowest was Kol Banda (3.3µg/ml). Keywords: Malabar Forest, Inventory, Leaf Chlorophyll.
Analysis of Hedonic Test and Total Microbial Plate Numbers on Fish Siomay in Sorong City West Papua Sukmawati Sukmawati
Bioscience Vol 4, No 2 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202042108256-0-00

Abstract

One of the Indonesian snacks that can be found in all regions is fish siomay. fish siomay in the city of Sorong, there have been no reports on both the level of community preference and food security. The purpose of this study was to determine the level of community preference and the total number of microbial plate numbers snacks on fish siomay in the city of Sorong, West Papua. The method used in this research is descriptive method and experimental method. The results showed that for the hedonic test had a varying degree of preference for each sample, while the total number of plates did not exceed the maximum limit, then for the correlation of the two tests showed there was a relationship. The hedonic test results from the five samples show the best level of preference is the BS sample, and the EH sample, then the AQ sample, and the DS sample and the lowest preference level is the CR sample. While the sample of fish siomay scattered in the city of Sorong was suitable for consumption. 
Serum Protein Total Determination After Hypoxic Swimming Exercises in Trained and Untrained Swimmers Using The Nanophotometric Method Siska Alicia Farma; Syahrastani Syahrastani
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151111936-0-00

Abstract

Anaerobic physical exercise aims to increase the explosive power, which is the ability of a person's muscles to do work with maximum strength in the fastest possible time. Sprint swimming exercises are often called hypoxic swimming exercises. Protein biomolecules have a major function in building muscle mass, maintaining body defences and increasing sports performance. Physical exercise occurs in a variety of systemic and cellular responses involving metabolic, immunological and hormonal changes. It is necessary to know how the total protein profile in serum after physical exercise in this case was limited to a single hypoxic swimming exercise. This was experimental research with post-test only control group design. There were three training groups, namely (5) control, (5) trained, and (5) untrained based on inclusion and exclusion criteria with the purposive sampling method. Single hypoxic swimming exercise (sprint) did along 200 m. The swimming training pattern was per 50 m without resting with two types of styles swim, freestyle and breaststroke. Measurement of total protein levels was carried out using a nanophotometer where the volume of serum used was very small. The results showed that the total protein content between the control group and the treatment group was significantly different (ANOVA, p <0.05). Between the trained and untrained groups, a decrease in total protein levels was seen in the trained group. This is probably due to the function of these proteins. It can be concluded that protein is more widely used by the body as a building element in the body, especially muscle mass. Cellular adaptive response is better when the body is in a trained condition.
Characteristics of Bird Nestling Trees in the Padang State University, West Air Tawar, West Sumatera Febrinal Febrinal; Fingki Alvia Chandra; Rijal Satria; Ramadhan Sumarmin
Bioscience Vol 4, No 2 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202042106098-0-00

Abstract

The purpose of this research is to describe the characteristic of the tree as birds nesting at State University of Padang , West Air Tawar, West Sumatra. This research conducted on March 2019. Data collection was using survey method. The results showed that found 14 species of tree is using by birds to nesting. The characteristic of tree is preferable by birds to using for nesting are tree and pole growth rate, monopodium and simpodium branching type, with round, oval and fountain canopy type, model leeuwenberg, attims and corner architecture model.      
Vivivary Tests on Mangrove Plants Species Rhizophora apiculata and Avicennia alba Against the Level of Salt Salinity and Tide Pandu Prabowo Warsodirejo; Tika Santika; RA Rina Dian Anggraini; Widya Sari
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151110894-0-00

Abstract

Indonesia's coastal areas have a large area and potential for mangrove ecosystems. Of the approximately 15,900 million ha of mangrove forests in the world. 27% or 4,293 million ha are in Indonesia. The Sicanang Medan Belawan mangrove forest, North Sumatra Province, is a wetland area which mostly consists of mangrove forests. In the mangrove forest, there are Rhizophora apiculata and Avicennia alba species. Mangroves have unique morphological characters as a form of adaptation to the environment, one of which is the salinity of sea water which is the level of salinity or salt content in seawater. In the mangrove forest of Sicanang Medan Belawan, there are Rhizophora apiculata and Avicennia alba species which have the characteristics of vivivar culture development. From the results of test 1 on the species Rhizopora apiculata, it was found that the highest salinity level at the muddy and sandy station was 30 ppm, and the highest pH was at 7.79, and at a temperature of 29 C. While for Avicennia alba the highest salinity value was obtained at 30.6 ppm, the highest pH was at 7.82. , and temperature at 29.5 C. Based on the results that different zoning could affect yield, the vivivary shape of mangroves also had an effect on yield. salinity affects mangroves to develop, the higher the salinity, the more difficult it is for the seeds to develop
Kombucha Tea Production by Amobil Cells in Several Different Tea Processing I. Irdawati; Putri Alamanda Sari
Bioscience Vol 4, No 2 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202042106062-0-00

Abstract

Kombucha is a fermented tea made from steeping tea and sugar which utilizes byproducts from bacterial and yeast symbiosis in the form of acidic compounds and nata (cellulose). Kombucha fermentation process produces a variety of substances that are efficacious to improve body health and ward off poisons. One method of kombucha fermentation can be done by cell immobilization method or enzymes because it can be repeated fermentation. The purpose of this study was to determine the quality of kombucha tea by immobilized cells in several different teas.This research is an experimental research. The research was conducted from May - August 2019 in the Microbiology Laboratory of the Faculty of Mathematics and Natural Sciences, Padang State University. The quality test of kombucha tea by immobilized cells can be determined by measuring the thickness of nata, pH, and total acetic acid of Kombucha tea. Data were analyzed using ANOVA followed by further tests DMRT with a level of 5%. The results showed that the best quality of kombucha tea by immobilized cells was green tea with a thickness of 0.53 cm nata, pH 5.62 and total acetic acid (%) 4.40.
Diversity of Medicinal Plant and Potential as Antibacterial in Peret Hill Forest, Tiang Tarah Village, District of Bakam, West Bangka Monica Kharisma Swandi; Neli Sulastri; Fitri Ulandari; Mega Febriyanti
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151111785-0-00

Abstract

Traditional medicine that has been done by the Tiang Tarah Village shaman, Bakam District, Bangka Regency uses plants as medicine to cure various diseases. However, so far some plants that are used as medicine can not be proven definitively effectiveness against a disease. Therefore, this research was conducted with the aim of knowing which plants are used as traditional medicine in Tiang Tarah Village, Bakam District, Bangka Regency, exploring the diversity of medicinal plants in the Bukit Peret Forest, and testing the antibacterial activity of medicinal plants against Escherichia coli and Staphylococcus aureus bacteria. The research was conducted with several stages, namely: (i) Interviewing informants who are experienced in the field of traditional medicine (shaman) using snowball sampling techniques, (ii) Exploration in the Peret Hill Forest for sampling using roaming method, (iii) Antibacterial activity test medicinal plants against E. coli and S. aureus by using agar diffusion method. The results of the study obtained 22 types of medicinal plants interviewed by 3 shamans Tiang Tarah Village. Of the 22 species, only 14 were found during exploration in the Peret Hill Forest. 10 of them have antibacterial activity against E. coli and 11 types against S.aureus.
The Effect Of Sargassum sp. Liquid Organic Fertilizer In The Growth of Spinach Plant (Amaranthus hybridus L.) by Using Hydroponic Syifa Fajrisani; Violita Violita; Irma Leilani Eka Putri; Des Des M
Bioscience Vol 4, No 2 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202042101106-0-00

Abstract

Abstract. The purpose of this research to determined the effect of Sargassum sp. liquid organic fertilizer in the growth of spinach plant (Amaranthus hybridus L.) using hydroponic. This research used Completely Randomized Design with six treatments and four repetitions ie: A= comparison (AB mix 10 mL/L of water), B= liquid organic fertilizer 6 mL/L of water, C= liquid organic fertilizer 8 mL/L of water, D= liquid organic fertilizer 10 mL/L of water, E= liquid organic fertilizer 12 mL/L of water, and F= liquid organic fertilizer 14 mL/L of water. Parameter observed was height of plants, number of leaves, leaf surface area, fresh weight of plants, and dry weight of plants. Data was analyzed by using the Analysis of Variants (ANOVA)  and Duncan. The result showed that application of Sargassum sp. liquid organic fertilizer has been  an effect on height of plants, number of leaves, leaf area, fresh weight of plants, and dry weight of spinach plant (Amaranthus hybridus L.). However, the effect was not as expected to increase the growth of spinach plant.

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