cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Dr. Masdiana C. Padaga, DVM. M App.Sc
Contact Email
vbcj@ub.ac.id
Phone
-
Journal Mail Official
nofanvet@ub.ac.id
Editorial Address
-
Location
Kota malang,
Jawa timur
INDONESIA
Veterinary Biomedical and Clinical Journal
Published by Universitas Brawijaya
ISSN : -     EISSN : 26221012     DOI : -
Core Subject : Health, Science, Education,
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Veterinary
Veterinary Clinical and Biomedical Journal (VBCJ) is periodically peer reviewed journal that publish every January and July per year since 2019. VBCJ accommodate any articles in the field of veterinary medicine, biomedical, as well as veterinary case report.
Arjuna Subject : -
Articles 6 Documents
 1 
Search results for , issue "Vol. 6 No. 1 (2024)" : 6 Documents clear
Effect of Piper crocatum Extract on Kidney Histopathology in Alloxan-Induced Wistar White Rats (Rattus norvegicus) Solfaine, Rondius; Rahmadhani, Diyana
Veterinary Biomedical and Clinical Journal Vol. 6 No. 1 (2024)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.01.2

Abstract

The prevalence of diabetes mellitus is steadily increasing. According to the International Diabetes Federation in 2021, the number of diabetes sufferers in Indonesia has rapidly increased in the last ten years. This study aims to determine the effect of red betel leaf extract (Piper crocatum) on kidney histopathology of Wistar white rats (Rattus norvegicus) induced by alloxan. A total of 25 white male Wistar rats aged ± 3 months were used in this study. All samples were divided randomly into five treatment groups, namely: (K-) healthy rats that were only given distilled water; (K+) given a single dose of alloxan 120 mg/kg bw/intraperitoneal; (P1) rats given alloxan 120 mg/kgbw/intraperitoneally and red betel leaf extract 100 mg/kg bw/orally; (P2) rats given alloxan 120 mg/kg bw/intraperitoneally and red betel leaf extract 150 mg/kg bw/orally; (P3) rats were given alloxan 120 mg/kgbw/intraperitoneally and red betel leaf extract 200 mg/kg bw/orally. Treatment was given for 14 days. Blood glucose levels were measured using the Glucometer (EZ Smart) colorimeter method. On the day 15th, all rat were euthanized and necropsied to remove the kidneys and then made into preparations with HE staining. The histopathological lesions observed were necrosis, degeneration, and inflammatory cell infiltration. Histopathological observations were carried out using a microscope with 40x magnification. Data resulting from observations were analyzed using SPSS 20 with Kruskal-Wallis and Mann-Whitney (P≤0.05). It revealed that red betel leaf extract has a potential ability to reduce the effects of hyperglycemia, and by inducing 2% of red betel leaf extract at dose of 100 mg/kg bw, 150 mg/kg bw, and 200 mg/kg bw could reduce necrosis, inflammatory cell infiltration, and degeneration significantly in the kidneys of white rats.
Management of Avian Influenza H9N2 Vaccination in Broiler Chickens at JAPFA COMFEED Myanmar Pte Ltd. Verian, Bagas Ridjalu; Prasetyo, Dodik; Adrenalin, Sruti Listra; Wuragil, Dyah Kinasih; Cahyo, Budi Nur
Veterinary Biomedical and Clinical Journal Vol. 6 No. 1 (2024)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.01.3

Abstract

Avian influenza virus subtype H9N2 (AIV-H9N2) has been a concern for poultry health in the last 20 years. The virus emerged in domestic poultry in the mid-1990s and has been reported in several Asian countries, including Myanmar. AI subtype H9N2 caused huge economic losses to the poultry industry in the form of respiratory distress and 25% weight loss in broiler chickens. This study discusses the administration of Avian Influenza (AI) H9N2 strain inactivated vaccine to commercial broiler chickens at JAPFA COMFEED Myanmar Pte Ltd. The vaccination process was carried out through subcutaneous injection in the neck and intramuscular injection in the pectoralis musculus in the chest area of chickens at 11 days of age, with a dose of 0.5 ml per chicken. The vaccine used was VAKSIMUNE® AI H9 which uses oil as the emulsion material. Results from the HI serology test showed that 6 cages from Myothar and Wah Net Chaung farms showed protective antibody titers against Avian Influenza H9N2 virus at 30-40 days of age, with normal titer values or ≥24. This indicates that the AI subtype H9N2vaccine used is an effective antigen, capable of stimulating the broiler immune system, and has implications for increasing antibodies every week. This study contributes to the understanding of AI H9N2 vaccination management in commercial broilers at JAPFA COMFEED Myanmar Pte Ltd. With positive results in the serology test, it can be concluded that vaccination using VAKSIMUNE® AI H9 is effective in protecting broilers from Avian Influenza H9N2 infection, providing strong support to the sustainability of production and animal health in a commercial farm environment.
Hepatoprotective Activity of Ethanol Extract of Dewa Leaves (Gynura pseudochina (L.) DC) in White Rats (Rattus norvegicus) Induced by Paracetamol Yelis Tria Monica; Listyawati, Shanti; Herawati, Elisa
Veterinary Biomedical and Clinical Journal Vol. 6 No. 1 (2024)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.01.4

Abstract

The use of paracetamol in excessive doses can increase the hepatotoxicity effect, which causes acute hepatocellular injury. Dewa leaves (Gynura pseudochina (L.) DC) contain flavonoids, saponin, and tannin. This study aimed to determine the effect of ethanol extract from dewa leaves in reducing the impact of paracetamol induction on SGPT and SGOT levels and liver histology of white rats (Rattus norvegicus). The test was grouped into five treatment groups, namely: normal control; negative control with paracetamol induction 315mg/KgBW; Dewa leaf extract was administered with three variations in dosage, namely 3.15mg/KgBW, 3.50mg/KgBW and 3.85mg/KgBW and each treatment group consisted of 5 rats as replications. Dewa leaf extract was administered to the experimental animal, followed by the administration of paracetamol 8 hours after the extract was given orally; both treatments were given for 14 days. Measurement of SGPT and SGOT levels uses the photometric method. The hepatoprotective effect was further verified by histopathology of the liver. Liver histology was made using the paraffin method. SGPT levels, SGOT levels, and Hepatocyte Damage Scores were analyzed using Analysis of Variance (ANOVA); if the results are significant, continue with the Least Significance Difference (LSD) test at a significance level of 5%. The results of this study show that the administration of dewa leaf extract was not able to significantly reduce SGPT and SGOT levels in rats. The administration of dewa leaf extract at doses of 3.50mg/KgBW and 3.85mg/KgBW significantly prevented histological damage to rat livers. 
Effects of Euthanasia on Animal Research Maharani, Ni Putu Radela; Fadlyah, Nur Ashfiya; Setyaningrum, Dyah Ayu Woro; Hairunisa, Nany
Veterinary Biomedical and Clinical Journal Vol. 6 No. 1 (2024)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.01.6

Abstract

The use of experimental animals is widely conducted in the medical field, both for research on the processes of disease occurrence (pathogenesis), the causes of disease (etiology), and research on therapy. Therefore, it is important to understand how euthanasia in experimental animals affects the integrity of the sample and its influence on research results. This article was compiled from several literature sources searched from Google Schoolar and PubMed published within the last 10 years, with keywords such as "code of ethics in experimental animals", "methods of euthanasia in experimental animals", "overdose anesthesia methods in animal euthanasia", "barbiturate injection methods in rats", "inhalation methods in animal euthanasia", "biochemical effects of euthanasia", and "histological effects of euthanasia". This paper discusses euthanasia methods, namely overdose anesthesia, inhalation, and injection. This paper aims to discuss how the method of euthanasia and the choice of drugs can impact the biochemical and histological changes in animals after the euthanasia process. Choosing the right euthanasia method is essential, as the species of animals involved and the research goals are considered. Using methods that align with the biological characteristics and behavior of the animals can help ensure the success of the research while also considering ethical aspects and animal welfare.
Effects of Lycopene Treatments on Development, Hatchability, and Heart Rate of Zebrafish Embryos under Heat Stress Exposure Tuska, Habib Syaiful Arif; Salsabila, Odifiannisa Ayu; Lonameo, Bonick Kartini; Hardian, Andreas Bandang; Lesmana, M. Arfan; Yesica, Reza
Veterinary Biomedical and Clinical Journal Vol. 6 No. 1 (2024)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.01.1

Abstract

Lycopene, a potent antioxidant predominantly found in red-hued fruits and vegetables such as tomatoes, has been incorporated into embryonic maturation media in vitro, demonstrating enhancements in embryo quality. Despite these advancements, the specific effects of lycopene on Zebrafish embryo quality remain unexplored. This study aims to investigate the impact of lycopene (0.65 ppm) on the development of Zebrafish embryos, focusing on hatchability (HA), and heart rate (HR) under conditions of heat stress (HS). Fertile Zebrafish embryos at the gastrula stage were induced HS (30oC; 1 hour). The embryos will be segregated into two groups: those exposed to HS and nonHS (28oC). Each group will receive one of three treatments (four replicates): control / without lycopene (P1), 2μL lycopene (P2), and 4μL lycopene (P3). Observations using an inverted microscope was done every three up to 48 hours post-fertilization (hpf), and subsequently every five hours up to 96 hpf. Quantitative data obtained for HA and HR were analyzed with SPSS, One-Way ANNOVA (p<0.05). The antioxidants contained in lycopene can influence the speed and success of Zebrafish embryo development and HA exposed to HS. In addition, the antioxidant in lycopene can also suppress the HR frequency of Zebrafish embryos exposed to HS. However, more studies about the optimum dose, purity, and stability of the lycopene extract should be done to make a definitive conclusion.
The Effect of Feeding and Feed Hygiene to Oxyurids Infection in Bearded Dragon (Pogona vitticeps) Indasari, Elly Nur; Budiman, Hafiidh Yahya; Nakita, Agnes Tageomi; Tsabitah, Azmi; Putra, Uray Ade Armanda
Veterinary Biomedical and Clinical Journal Vol. 6 No. 1 (2024)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.01.5

Abstract

Bearded dragons (Pogona vitticeps) are popular exotic pets. Oxyurid can be transmitted to them through contaminated insect and vegetable feed that may carry infective larvae and worm eggs. Oxyuriasis frequently infects Bearded dragon which then causes diarrhea, weight loss, and other digestive issues. This study investigated the impact of various feeding practices and feed hygiene on the prevalence and severity of Oxyurid infections in Bearded dragon. Total of 24 Bearded dragon was used on this study. The variation of feed was used in groups include mixed vegetable and insect feed without hygiene procedures (P0), insect feed without hygiene procedure (P1), vegetable feed without hygiene procedure (P2), mixed vegetable and insect feed with hygiene procedures (P3). For P3, the insects' stomach contents were removed and washed, while the vegetables were rinsed under running water and then dried before being fed to the reptiles. The morphology of Oxyurida eggs found in each group was examined using native and flotation techniques, while, the McMaster technique was carried out for infection intensity measurement. Data observed was analyzed using the Mann-Whitney test to evaluate the significance of the treatment effects, and the Kruskal-Wallis test to examine the associations. It showed that Pharyngodon sp. was found in all feed groups. A significant relationship (P < 0.05) was found between feed type, feed hygiene, and the infection rate of Pharyngodon sp. Conversely, the group which received the same type of feed but adhered to stringent feed hygiene protocols exhibited a significant fewer infection. This study highlights the critical importance of proper feeding management and feed hygiene in maintaining the health of Bearded dragon, particularly in preventing oxyurida infections

Page 1 of 1 | Total Record : 6

 1 

Filter by Year

2024 2024


Reset
Filter By Issues
All Issue Vol. 7 No. 1 (2025): Vol. 7 No. 1 2025 Vol. 6 No. 2 (2024): Vol. 6 No. 1 (2024) Vol. 5 No. 2 (2023) Vol. 5 No. 1 (2023) Vol. 4 No. 2 (2022) Vol. 4 No. 1 (2022) Vol. 3 No. 2 (2021) Vol. 2 No. 2 (2020) Vol. 1 No. 2 (2019) Vol. 1 No. 1 (2019) More Issue