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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,328 Documents
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COMPARISON OF HPV DETECTION USING HC-II METHOD WITH PAP SMEAR SCREENING IN COMMERCIAL SEX WORKERS IN KEDIRI Erawati Erawati; Puspa Wardhani; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 3 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i3.1336

Abstract

Female commercial sex workers are females that have multiple sexual partners and have high risk due to exposure to blood, semen, and vaginal discharge contaminated with microorganisms causing sexually transmitted disease such as infection caused by Human papillomavirus (HPV). This behavior creates a high susceptibility for commercial sex workers in obtaining HPV, which is the leading cause of cervical cancer. Cervical cancer is the most common cancer in females in Indonesia, which is why screening, especially for females with a high risk such as commercial sex workers, must be done. The purpose of this experiment was to compare the detection methods of HPV using Hybrid capture-II (HC-II) in order to find out high risks HPV types (type 16, 18, 31, 33, 35, 39,45, 51, 52, 56, 58, 59, and 68) by Pap smear done in commercial sex workers in Campurejo Kediri Public Health Center. This study was a descriptive observational experiment with a cross-sectional method. The samples of this experiment were 47 female commercial sex workers, whose detection of HPV using HC-II method was done at the Clinical Pathology Laboratory of the Dr. Soetomo Hospital Surabaya, where 32 samples showed positive results (68.1%) and were infected with high-risk HPV and 15 negative results (31.9%), from the Pap smear three samples (6.4%) showed dysplasia (Cervical Intraepithelial Neoplasia/CIN 1) and 44 samples (93.6%) showed normal smears with inflammation or infection in the cervix. Statistically showed a significant difference between the results of HC-II and Papsmear (p=0.000). 
APPLICATION OF DNA METHYLATION ON URINE SAMPLE FOR AGE ESTIMATION (Penggunaan Metilasi DNA dalam Perkiraan Umur Individu di Sampel Air Kemih) Rosalinda Avia Eryatma; Puspa Wardhani; Ahmad Yudianto
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 1 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i1.1179

Abstract

Perkiraan umur sangat penting dalam analisis forensik. Umur individu lebih sering diperkirakan dengan menggunakan tulangdan gigi. Namun, hanya terbatas pada kasus tertentu yang berhubungan dengan kerangka manusia. Metilasi DNA merupakan salahsatu cara dalam memperkirakan umur di sampel biologis. Perkiraan umur menggunakan metode metilasi DNA dengan penggunaansampel air kemih hingga saat ini belum pernah ada yang melakukan, oleh sebab itu penelitian ini akan mengunakan isolasi metilasiDNA dalam memperkirakan umur individu di sampel airkemih. Di penelitian ini digunakan 6 sampel air kemih yang didapatkan daripendonor sehat. Tahap pertama adalah isolasi DNA dengan menggunakan DNAzol dan kloroform setelah itu, dikonversi bisulfit dengankit DNA metilasi. Hasil isolasi kemudian di amplifikasi dengan metode PCR dan di elektroforesis dengan gel agarosa. Hasil elektroforesisdapat sebagai acuan panjang pita yang di sekuensing. Hasil sekuensing dianalisis persen metilasinya dan korelasinya dengan perkiraanumur. Hasil dari pembacaan aplikasi dan perhitungan tersebut di sampel 001 menunjukkan 64,99%, sampel 002 menunjukkan 69,45%,sampel 003 menunjukkan 57,52%, sampel 4 menunjukkan 58,61%, sampel 005 menunjukkan 63,66% dan sampel 006 menunjukkan61,19%. Berdasarkan hasil penelitian ini merupakan langkah awal dalam penggunaan metilasi DNA dalam perkiraan umur individu terutamadi sampel air kemih.
SEKUENS TERAWETKAN TERKAIT HIV-1 Efrida Efrida; Andani Eka Putra
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1081

Abstract

Human Immunodeficiency Virus-1 (HIV-1) have the high levels of genetic variability (molecular variation) related to the mechanismof deletions, insertions, and especially as a recombination. The recombination between subgroups and their inter subtype HIV-1 producesa new virus strain causing a certain infection. Until now there were about 40 circulating recombinant form (CRF) and 100 uniquerecombinant forms (URF) worldwide. Various HIV-1 molecular variation cause pitfalls HIV detection due to the difficulty test designed todetect all HIV-1 strains. The purpose of this study was to know the determination of the molecular variation that can identify the HIV-1genome sequences which are conserved from local isolates of West Sumatera. The method used is a descriptive study on samples of patientssuspected of being infected by HIV-1 based on the antibody screening results using rapid HIV-1 test in the laboratory of Clinical Pathologyat Dr. M. Djamil Hospital Padang. The RNA isolation and amplification by RT-PCR was performed at the Microbiology Department,Faculty of Medicine of UGM. The amplicons were sequenced using the Sanger method and analyzed by Clustal W with sequence datafrom the HIV its database. The major subtypes of HIV-1 isolates derived from local West Sumatra is AE/B with four (4) isolates (57.1%),followed by subtype AE, 2 isolates (28.6%) and B, 1 isolates (14.3%). Most of the molecular variation in this study related to thesubstitution of one base followed by deletions and insertions. The protease showed a wide variation, which consists of 20–37 substitutionsand 1–3 deletions. p24 is a highly conserved gene, followed by gp120 and vpu. Based on this study, it can be concluded that the isolatesof HIV-1 recombination is the biggest proportion with conserved region found at p24 of protein group of the gag.
RERATA VOLUME TROMBOSIT DAN AGGREGASI TROMBOSIT DI DIABETES MELITUS TIPE 2 (Mean Platelet Volume and Platelet Aggregation in Diabetes Mellitus Type 2) Malayana Rahmita Nasution; Adi Koesoema Aman; Dharma Lindarto
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i3.1283

Abstract

Diabetes mellitus patients often have hypercoagulable blood, as evidenced by the increased coagulation, impaired fibrinolysis,endothelial dysfunction and platelet hyperactivity. Hyperactive platelet is the major determinant of pro thrombotic state in DM. Byassessing the MPV and platelet aggregation, which is a marker of platelet activity, in patients with type 2 DM, it is expected to help theprediction of acute events. This research is aimed to know the differences of MPV and the aggregation of platelet between poor glycemiccontrol as well as good the control group in type 2 DM patients. This study was conducted in cross sectional method using 22 people withgood glycemic control and 28 people with poor one (glycemic control) from June to August 2013. Fasting blood samples were analyzedfor CBC, HbA1c, TG and platelet aggregation. MPV and platelet aggregation value were compared between groups using independentt-test. Based on this study, there is no significant difference in MPV and platelet aggregation between groups (p=0.598, p=0.464 (1 μM),p=0.868 (2 μM), p=0.984 (5 μM), p=0.401 (10 μM)). Mean Platelet Volume (MPV) correlate significantly with platelet aggregationat 1 μM and 5μM ADP concentration in good glycemic control group (r=0.591; p=0.004 at 1 μM ADP and r=0.521; p=0.013 at 5 μMADP). Mean platelet volume correlate significantly with the platelet aggregation at 2 μM ADP and the concentration in poor glycemiccontrol group (r=0.405; p=0.033). There are no significant differences in MPV and platelet aggregation between groups, but there is asignificant correlation between them (MPV and platelet aggregation) in the good glycemic control of the type 2 DM group.
CORRELATION OF NEUTROPHILS/LYMPHOCTES RATIO AND C-REACTIVE PROTEIN IN SEPSIS PATIENTS Henny Elfira Yanti; Fery H Soedewo; Puspa Wardhani
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 2 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i2.1143

Abstract

Sepsis merupakan penyebab umum dari kesakitan dan kematian di seluruh dunia. Diagnosis yang cepat dan tepat sangatdiperlukan. Salah satu respons fisiologis pada sistem imunitas terhadap inflamasi sistemik adalah peningkatan jumlah neutrofil danpenurunan jumlah limfosit. Rasio neutrofil/limfosit telah diketahui sebagai petanda inflamasi yang cepat, sederhana dan murah. Tujuanpenelitian ini adalah membuktikan adanya kenasaban antara rasio neutrofil/limfosit dan c-reactive protein di pasien sepsis. Penelitianbersifat ptong lintang observasional, dilakukan masa waktu Januari hingga Juni 2015. Sampel penelitian terdiri dari 42 pasien sepsisyang dirawat di Instalasi Rawat Darurat RSUD Dr. Soetomo Surabaya. Semua sampel dilakukan pemeriksaan jumlah limfosit, neutrofil,CRP. Hasil dianalisis menggunakan uji kenasaban Spearman’s. Rasio neutrofil/limfosit berkisar antara 3,42–57,47 (21,74±14,1). KadarCRP antara 1,22 mg/L–361,86 mg/L (158 mg/L±97,4 mg/L). Hasil penelitian tidak terdapat kenasaban antara RNL dan kadar CRPdi pasien sepsis (p=0,51). Tidak terdapat kenasaban antara RNL dan kadar CRP di pasien sepsis. Hal ini disebabkan adanya perbedaanjalur aktivasi antara neutrofil dengan CRP sehingga menyebabkan peningkatan CRP tidak sebanding dengan peningkatan jumlahneutrofil.
INDEKS ATEROGENIK PLASMA DI INFARK MIOKARD AKUT DAN PENYAKIT DIABETES MELITUS (Atherogenic Index of Plasma in Acute Myocardial Infarction and Diabetes Mellitus) Zulfikar Indra; Suci Aprianti; Darmawaty E.R.
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i3.1271

Abstract

Diabetes Mellitus (DM) is associated with an increased cardiovascular risk. Atherogenic Index of Plasma (AIP) is a strong predictorof myocardial infarction. The aim of this study was to know the difference of AIP in acute myocardial infarction (AMI) with and withoutDM. This study was conducted by cross sectional method using data from the medical records of AMI patients at the Dr. WahidinSudirohusodo Hospital, Makassar during January 2010 up to May 2013. The AIP values were calculated by the [log (TG: HDL-C)]formula. All data were classified into two groups. The data were then analyzed by unpaired T test. In this study, AMI was mostly foundin the 50–59 years group, 33.63%. The AIP in AMI with DM was higher than without DM (0.69±0.26 vs 0.57±0.26, p=0.001). TheAIP in AMI with and without DM was higher in the female than the male group (0.63±0.24 vs 0.62±0.28. P=0.58). The AtherogenicIndex of Plasma in AMI with DM was higher than without DM. Based on this study, it can be concluded, that AIP can be used as thepredictor of cardiovascular risk in diabetes patients.
NILAI BATAS ANTIGEN NS1 DENGUE KUANTITATIF SEBAGAI PREDIKTOR KEPARAHAN JANGKITAN/TULARAN (INFEKSI) VIRUS DENGUE ANAK Betty A Tambunan; Aryati .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1044

Abstract

The diagnosis of viral dengue infection is very important for the management of dengue patients. In acute phase infection circulatingNS1 antigen can be detected in the sera of patients with dengue viral infection. This study is evaluating the NS1 antigen level in denguepatients using antigen captured ELISA Platelia TM Dengue NS1 Ag (Bio-Rad Laboratories). In this 30 examined dengue patients consistingof 3(10%) undifferentiated fever, 10(33.33%) dengue fever, 12(40%) DHF grade I, 2(6.66%) DHF grade II, 2(6.66%) DHF gradeIII, 1(3.33%) DHF grade IV. The result revealed that NS1 antigen was positive in 12 among 30 patients (40%) which were diagnosedas Dengue Viral infection based on 1997 WHO criteria. The sensitivity of NS1 antigen in these patients as confirmed with IgM andIgG antidengue serology test was 52.2%. The highest positivism of NS1 antigen was on the third day of fever. The results analyzed bySpearman correlation test revealed that there was no significant correlation between NS1 antigen level and the severity of dengue viralinfection. The cut-off value of quantitative NS1 antigen could not be determined because they were no significant correlation shown forNS1 antigen as the predictor for the severity of dengue viral infection. The conclusion of the study so far shown that the quantitativeNS1 antigen level could not be used as the predictor for the severity of dengue viral infection. The cut-off value of quantitative NS1antigen could not be determined because there were no significant correlation shown for NS1 antigen as the predictor for the severityof dengue viral infection.
PERBEDAAN GOLONGAN DARAH ABO DI ANEMIA HEMOLITIK AUTOIMUN Hilma Yuniar; Rachmawati Muhiddin; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.473

Abstract

Discrepancy of blood type is the difference result between cell grouping and serum grouping. The technical errors and various conditions are the common cause of their discrepancy, one of the conditions is mentioned as Auto Immune Haemolytic Anaemia (AIHA). Auto immune haemolytic anemia cold type can cause discrepancy due to cold autoantibody reaction. In this study the researchers conducted further testing to determine the actual blood type so the right blood transfusion can be given. A 41 years old woman with weakness and pale since a month before her admission to the present hospital, has been treated in a district hospital with the same condition. On the physical examination, the researchers found hepatomegaly. The complete blood count showed hemoglobin level was 2.1 g/dL and leukocyte count was 21.54×103/uL. The clinical diagnosis made by the emergency department was anemia pro evaluation and planned blood transfusion with 500 cc of PRC. The peripheral blood smear shown hemolytic anemia. The result of the initial blood type test on forward grouping were AB positive agglutination and on reverse grouping O positive, then followed by washing erythrocyte and the researchers found the similar result. Because of the extra antibody found in both examination, the examination was carried out at 37°C of the patient’s temperature. The researchers found that the blood type of AB rhesus positive with cold autoantibody. The indirect coombs test was positive. The patient is had been reported with ABO discrepancy cold type autoantibody (group IV) in autoimmune hemolytic anemia cold type. Further screening antibody tests are needed to confirm the specific antibody.
THE RISK FACTOR OF ALLOANTIBODY FORMATION IN THALASSEMIA PATIENTS RECEIVING MULTIPLE TRANSFUSION (Faktor Kebahayaan Terbentuknya Aloantibodi pada Pasien Talasemia yang Menerima Transfusi Darah Berulang) Fridawati, Veronica; Triyono, Teguh; Sukorini, Usi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 3 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i3.1239

Abstract

Untuk kelangsungan hidup pasien talasemia intermediet dan mayor, memerlukan transfusi darah secara teratur. Transfusi berulangini berpeluang membentuk aloantibodi yang dapat menyebabkan kebahayaan hemolitik. Maka transfusi berulang akan memperberathemolitik karena pada pasien talasemia sudah ada proses tersebut. Tujuan penelitian ini adalah untuk mengetahui berbagai faktorkebahayaan untuk terbentuknya aloantibodi pada pasien talasemia yang mendapat transfusi darah berulang khusus di RSUP Fatmawati,Jakarta. Cara meneliti ini menggunakan rancangan potong lintang. Subjek penelitian adalah semua pasien talasemia yang mendapattransfusi darah berulang di RSUP Fatmawati Jakarta yang memenuhi patokan kesertaan. Sebanyak 81 subjek diikutkan dalam penelitianini. Data pada penelitian ini di analisis secara statistik dengan uji Chi Kuadrat. Hasil menguji secara Chi Kuadrat menunjukkan: kelamin,suku, diagnosis, selang transfusi darah, jenis darah, reaksi yang terkait, riwayat keluarga, kadar Hb. Kadar feritin dan golongan darahbukan merupakan faktor kebahayaan untuk terbentuknya aloantibodi, sedang faktor usia, jumlah kantong darah yang ditransfusikan,keberadaan komplikasi akibat transfusi darah dan lama masa waktu menerima darah transfusi, merupakan faktor kebahayaan untukterbentuknya aloantibodi pada pasien talasemia yang mendapat transfusi berulang di RSUP Fatmawati.
KADAR KREATININ DAN BERSIHAN KREATININ PENDERITA LEPTOSPIROSIS Ismawati Amin; B. Rusli; Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 13, No 2 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v13i2.882

Abstract

Leptospirosis is a frequent zoonosis in the tropics including Indonesia with 5–30% incidence and 5–20% mortality rate due toacute renal failure (ARF). To determine the frequency of leptospirosis by sex, age and glomerulus’s filtration rate (GFR) of the patient.A retrospective descriptive study on 32 subjects was done by measuring the creatinine clearance using Lyasis Auto analyzer in ClinicalPathology Laboratory of Wahidin Sudirohusodo Hospital from January 2004 to June 2006, of 32 patients, 25 (78%) male and 7 (22%)female, with the most frequent age range from 30–40 years (34.4%). Of the patients, 2 (6.3%) have normal GFR, 6 (18.8%) withmild GFR reduction, 10 (31.3%) with medium GFR reduction, 10 (31.3%) with severe GFR reduction and 4 (12.5%) with acute renalfailure. Leptospirosis is most frequent found in male with age between 30–40 years old. with reduction of GFR from mild to severe, andeven terminated with acute renal failure.

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