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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,328 Documents
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ELEVATED SERUM S100B PROTEIN LEVEL AS A PARAMETER FOR BAD OUTCOME IN SEVERE TRAUMATIC BRAIN INJURY PATIENTS Ridha Dharmajaya; Dina Keumala Sari; Ratna Akbari Ganie
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 1 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i1.1159

Abstract

Beratnya suatu cedera kepala akibat trauma akan membuat gangguan saraf pusat. Kerusakan saraf ini dapat dinilai dengan petandabiokimia yang tepat. Pemakaian petanda biokimia terhadap kerusakan otak mendapatkan perhatian yang banyak terutama ProteinS100B. Protein S100B adalah suatu ikatan kalsium dan protein yang meningkat cepat sesaat setelah cedera kepala. Kesulitannya adalahuntuk memastikan, berapa lama Protein S100B ini harus diukur. Jika berhubungan dengan kerusakan otak, ia tidak selalu terjadi pada24 jam pertama. Dapat terjadi pada 48–72 jam pasca cedera kepala, bahkan 120 jam pada kecederaan tersebut. Penelitian ini bertujuanuntuk mendapatkan kenasaban antara Protein S100B dengan GOS sebagai faktor peramalan yang akurat, mudah, tidak menyakitkan,untuk cedera kepala berat. Pengambilan serum darah untuk pemeriksaan kadar Protein S100B dilakukan pada 24, 48, 72 dan 120 jampasca trauma. Selanjutnya pengukuran dilakukan dengan menggunakan Enzyme Linked Immunosorbent Assay (ELISA). Keluaran pasienpasca perawatan dinilai menggunakan penggolongan Glasgow Outcome Scale (GOS), tiga bulan pasca kecederaan. Hasil pengukurankadar Protein S100B pada 120 jam pasca cedera kepala berat menunjukkan hubungan berlawanan yang kuat terhadap keluaran pasien.Pasien cedera kepala berat dengan kadar Protein S100B 120 jam pasca trauma yang tinggi, memiliki hasil keluaran yang buru
KETOASIDOSIS DIABETIK DI DIABETES MELITUS TIPE 1 Zuhrinah Ridwan; Uleng Bahrun; Ruland DN Pakasi R
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i2.1127

Abstract

Type 1 Diabetes Mellitus (DMT1) is an autoimmune disease characterized by impaired carbohydrate, protein and lipid metabolismresulting in insulin deficiency either absolute or relative. The absolute insulin deficiency found in DMT1, is caused by a defect in thepancreatic ß cells leading to a defect in insulin synthesis by the pancreas gland. In this article, a case of type 1 DM in a child withketoacidosis diabetes has been reported. The diagnosis was based on classical symptoms, blood glucose level, C-peptide, metabolic acidosisand keton uria. The case reported is a 12 year old girl, with main complaints of 9 kg body weight loss since three (3) years ago. Thepatient always felt hungry, thirsty and was frequently urinating. In her family history no such case was denied. The patient had beentreated in the district general hospitals for five (5) days and was diagnosed as type 1 diabetes mellitus and was then referred to the Dr.Wahidin Sudirohusodo Hospital. The laboratory test results revealed as follows, random glucose level was 729 mg/dL, arterial blood gasanalysis showed metabolic acidosis, C-peptide was 0.1 ng/mL and urinalysis showed 150 mg/dL protein, 1000 mg/dL glucose, 150 mg/dL ketone and 250 RBC/uL. The patient was diagnosed as type 1 diabetes mellitus with diabetic ketoacidosis and received 0.5 U/kg/dayinsulin therapy. This case report aimed to determine the type 1 diabetes mellitus in a child is accompanied by diabetic ketoacidosis.
CORRELATION BETWEEN MATRIX METALLOPROTEINASE 1 SERUM LEVELS AND MODEL OF END STAGE HEPATIC DISEASE SCORE IN PATIENTS WITH HEPATIC CIRRHOSIS Stephanus Yoanito; Siti Muchayat
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 3 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i3.1195

Abstract

Sirosis hati masih menjadi masalah kesehatan utama di dunia, merupakan penyakit fibrosis hati yang berkebahayaan komplikasi.Pemeriksaan biopsi hati masih merupakan baku emas dalam penegakan diagnosis sirosis hati, tetapi pemeriksaan ini bersifatmenyakitkan. Banyak model prognostik telah dikembangkan dalam dua dekade terakhir untuk meramalkan kematian di pasien sirosishati dan variabel yang termasuk dalam model ini adalah Model End Stage Hepatic Disease (MELD). Penelitian ini mengukur kadarMatrix Metalloproteinase 1 (MMP-1) serum sebagai enzim dalam proses degradasi matrix extraselular pada sirosis hati, yang dapatdigunakan untuk menilai tingkat keparahan di pasien sirosis hati. Tujuan penelitian ini adalah mengetahui korelasi kadar MMP-1 serumterhadap skor MELD di pasien sirosis hati. Penelitian ini menggunakan rancangan observasional potong lintang. Ciri subjek ditampilkansecara deskriptif dalam perbandingan. Ciri hasil laboratorium dalam bentuk rerata, simpang baku, median (minimum-maksimum).Uji Spearman digunakan untuk mengetahui kenasaban kadar MMP-1 serum terhadap skor MELD di pasien sirosis hati. Sebanyak 30pasien sirosis hati, rerata umur 52,43 tahun, dominasi laki-laki sebesar 19 pasien (63,3%), penyebab terbanyak adalah HBV sebesar18 pasien (60,4%), dengan nilai median skor MELD adalah 15,5 dan kadar rerata MMP-1 adalah 106,91 pg/mL. Terdapat kenasabannegatif moderate antara kadar MMP-1 serum dengan skor MELD dan bermakna dengan nilai r=- 0,402 (p=0,028). Terdapat kenasabannegatif moderate dan bermakna antara kadar MMP-1 serum dengan skor MELD di pasien sirosis hati. Untuk aplikasi klinis pemeriksaanMMP-1 serum dapat digunakan untuk memantau hasil pengobatan dan perjalanan penyakit pasien sirosis hati.
ANTIGEN OMP (OUTER MEMBRANE PROTEIN) salmonella typhi FAGA LOKAL YANG IMUNODOMINAN DAN SPESIFIK TERHADAP ANTIBODI PENDERITA DEMAM TIFOID J. Nugraha; Rahayu Anggraini
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 13, No 3 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v13i3.912

Abstract

Background of this research is that diagnostics of typhoid fever is still a health problem. Widal test, which is the mostly used test in Indonesia, frequently gives false positive results and is not reliable in endemic areas. On the other hand, the other confirmation test, bloodculture, is not sensitive and often give false negative results. OMP (Outer Membrane Protein) is known as a specific part of Salmonellatyphi and fragments of OMP still exist in the patient’s body since early infection until 2–3 weeks thereafter. In this study parts of OMPwhich react specifically with sera of typhoid fever in Indonesia were searched. These specific parts will then be developed as a diagnostickit for typhoid fever. Using Western Blot method, part of OMP will be searched, which is specifically react with sera of typhoid feverpatients in Indonesia. OMP derived from local phage type isolated in Indonesia was used. This OMP was separated with SDS-PAGE 12%and incubated with pooled sera of typhoid fever patients, and sera of control group, that is from Dengue haemorrhagic fever patientsand urinary tract infection with E. coli. Extraction of OMP was done by the method of Matsuyama. Contrary, this research failed to find a particular part of OMP which react specifically with sera of typhoid fever patients. There are certain parts of OMP which react also with sera of DHF & urinary tract infection patients. Our finding was different with the results from Malaysia, where it is reported thatantigen OMP 52 kD react specifically there. In order to develop a diagnostic tool for typhoid fever, we should consider another possiblespecific antigen other rather than using OMP.
Correlation between Lactic Acid Concentration and The Severity of Neonatal Sepsis Iskandar, Agustin; Pranidya, Nada Putri; Sulistijono, Eko; Aryati, Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 1 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i1.1370

Abstract

BackgroundsNeonatal sepsis remains a major cause of high infant mortality rate in Indonesia, and served as one of risk factor for early neonatal mortality. This study aims to determine the relationship between lactate levels andthe severity of sepsis and prognostic value of lactic acid as a predictor of severity in neonatal sepsis.. MethodsThis research is analytic observational research using cross sectional method. The subjects were patients of neonatal sepsis treated in the Perinatology Room of Saiful Anwar Hospital Malang from February to June 2015. Lactic acid concentration in plasma was measured by using enzymatic colorimetric  methodResults and DiscussionThe results showed a positive and significant correlation (p = 0,023; r= o,414) between lactate concentration and severity of sepsis, where every increase of sepsis degree increased lactate level of 0,151 mmol / L. Whereas by using  cut off ≥ 2.5 mmol / L, the prognostic test showed  66.7% of sensitivity and 76.2% of specificity. The chi square test of lactate> 2 mmol / L and <2 mmol / L showed Odd Ratio (OR) of 1,3 whereas at lactate level > 5 mmol / L and <5 mmol / L showed OR of 4,8 indicated that although there was no difference but the mortality outcomes of neonatal sepsis 4.8 times greater those of life. Thus lactate levelsof > 5 mmol / L can determine mortality outcome of neonatal sepsis.Conclusions and suggestionsThe higher the lactic acid level the more severe of neonatal sepsis. Lactate levels greater than 5 mmol / L was predictor for determining mortality outcome in neonatal sepsis
KESAHIHAN PEMERIKSAAN COMPLEX SPECIFIC COCKTAIL ANTIGEN TB (ESAT-6, CFP-10, MPT-64) METODE CEPAT IMMUNOCHROMATOGRAPHY PADA CAIRAN SEREBROSPINAL PASIEN MENINGITIS TUBERKULOSIS {Validity of Rapid Immunochromatography Complex Specific Cocktail Antigen TB (Esat-6, Cfp-10, Mpt-64) Using Cerebrospinal Fluid of Tuberculous Meningitis Patient} Livia Noviani; Ida Parwati; Ganiem AR; Turbawati DK
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 1 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i1.1222

Abstract

The early diagnosis of definite tuberculous meningitis (TBM) is very important in reducing its mortality. The current gold standard ofTBM relies on the isolation of M. tuberculosis from cerebrospinal fluid (CSF) either with direct staining or M. tuberculosis culture, but theseexamination have a low sensitivity due to the pausibasilary condition. Recently there is an assay using rapid Immunochromatography(ICT) cocktail antigen TB in CSF to diagnose TBM. This method can detect ESAT-6, CFP-10 and MPT-64 antigen as an important virulencefactor for the spreading of bacteria to extra pulmonary which is secreted by M. tuberculosis in CSF from TBM patient. The aim of thisstudy was to know the validity of rapid ICT cocktail antigen TB using CSF against MODS culture and acid-fast bacili as a gold standardto diagnose TBM by analyzing. This study iscarried out by a descriptive observational study using cross sectional study design. Thesubjects are patients who were diagnosed as suspected TBM based on Marais criteria and were obtained from the Department of NeurologyHospital Dr. Hasan Sadikin. The examination was done at the Clinical Microbiology Department of Clinical Pathology Dr. Hasan Sadikinhospital since January 2014 until May 2014. A total of 41 subjects which consisted of six (6) subjects with a definite diagnosis of TBM,26 with probable TBM and nine (9) with possible TBM were enrolled in this study. The result of this assay againts acid-fast bacili has the100% sensitivity, 64.1% specificity, 12.5% PPV, 100% NPV, LR(+) 2.78, LR(–)0 and 65.8% accuracy. The result of this assay againtsM. tuberculosis culture has the 83.3% sensitivity, 68.5% specificity, PPV 31.2%, NPV 96%, LR(+) 2.65, LR(–)0.24, accuracy 70.7% andprevalence ratio 7.8. Based on this study, it can be concluded that the validity of this assay againts acid-fast bacili has a high sensitivity,moderate specificity, low PPV, high NPV and moderate accuracy. The result of this assay againts M. tuberculosis culture has a moderatesensitivity and specificity, low PPV, high NPV and moderate accuracy.
COMPARISON OF HBA1C LEVEL USING TURBIDIMETRY INHIBITION IMMUNOASSAY, LATEX AGGLUTINATION INHIBITION METHOD AND HPLC METHOD Salmon Sutandra; Asvin Nurulita; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 3 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i3.1409

Abstract

According to the National Glycohemoglobin Standardization Program (NGSP) and The Diabetes Control and Complication Trial(DCCT) the standard method of measuring HbA1c is High-Performance Liquid Chromatography (HPLC), but HPLC requiresparticular instrument investments, trained staff, long and relatively expensive sample processing. This an instrument that hassimilar performance to HPLC but its operation process is relatively simple and not costly. The purpose of this study was to analysethe HbA1c level using Turbidimetry Inhibition Immunoassay (TII) method and the HPLC method; to analyse HbA1 level using LatexAgglutination Inhibition (LAI) method and HPLC method. This research was conducted with a cross-sectional design during theperiod of March-April 2017. The total sample consisted of 160 samples divided into two groups. For the first group, HbA1c levelusing TII method and HPLC method was measured. For the second group, HbA1c level was measured using LAI method and HPLCmethod.The data obtained were analyzed using the Paired T-test. There was a significant difference between HbA1c levels usingTII method and HPLC method {8.12(2.53)% vs. 8.49(2.63)%, p<0.001}. There was no significant differences between HbA1c levelsusing LAI method and HPLC method {7.52(1.95%) vs. 7.47(2.00)%, p>0.05}. This research concluded that there was a differencebetween the HbA1c levels using TII method and HPLC method, but no difference between HbA1c levels using LAI method and HPLC method.
Analysis of Liver Fibrosis Degree with APRI Score and FIB-4 Index on Patients with Non-Alcoholic Fatty Liver Disease Gillian Elvira Seipalla; Nurahmi Nurahmi; Ibrahim Abd Samad
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 2 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i2.1414

Abstract

Liver fibrosis is the accumulation of Extracellular Matrix Protein (EMP) scar tissue after acute or chronic liver injury. Liverbiopsy is the gold standard test for evaluating liver fibrosis in patients with Non-Alcoholic Fatty Liver Disease (NAFLD). Thisdiagnostic method is invasive, painful, and complicated in rare cases; thus the noninvasive methods such as laboratory testsand radiology had been proposed to assess liver fibrosis in NAFLD and are expected to overcome the limitations of liverbiopsy. This method consists of aspartate Aminotransferase to the Platelet Ratio Index (APRI) and fibrosis-4 (FIB-4).Thisresearch was retrospective cross-sectional performed at Dr. Wahidin Sudirohusodo Hospital Makassar involving 63 subjectsfrom January to June 2018. The correlated variables were presented in categorical and numerical data and the Pearsoncorrelation test was used to determine the compatibility of APRI score and FIB-4 index with Fibroscan result in patientswith NAFLD. A highly significant positive correlation (p <0.001) was found between APRI score and Fibroscan (correlationvalue = 45.8%), APRI score and FIB-4 index (correlation value = 91.8%), FIB-4 index and Fibroscan (correlationvalue = 47.6%). The score of Aminotransferase to platelet ratio index score and FIB-4 index could be an alternative methodinstead of a liver biopsy to predict the degree of fibrosis in patients with NAFLD.
C-Reactive Protein Levels of Sepsis Patients: A Comparison of Three Immunoassay Methods Devi Rahmadhona; Aryati Aryati; Hardiono Hardiono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 1 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i1.1346

Abstract

Quick Sequential Organ Failure Assessment (qSOFA) is a modification of the SOFA score that replaces the Systemic Inflammatory Response Syndrome (SIRS) criteria for sepsis diagnosis. C-reactive protein (CRP) is a marker to help diagnose sepsis. There are not many studies about comparison of CRP level with a variety of instruments and methods, currently. This study aimed to analyze differences in CRP results with particle enhanced turbidimetric immunoassay (PETIA), sandwich immunodetection and reflectometry-immunoassay patients. The study used samples of sepsis patients who were treated in emergency care unit, intensive observation rooms, Intensive Care Unit (ICU) and internal medicine wards of the Dr. Soetomo Hospital Surabaya in May-September 2018. A total of 65 sampels of sepsis patient fulfilled the qSOFA criteria. The CRP examination with the three methods were conducted on all study samples. There were significant differences in CRP levels in the sepsis group using the PETIA and Reflectometry immunoassay methods (p = 0.003), thus both of methods cannot be replace each other. There was no significant difference between CRP levels with PETIA and Sandwich Immunodetection (p=0.172) as well as Reflectometry immunoassay and Sandwich Immunodetection (p=0.251). The selection of instruments and methods for CRP examination is adjusted to laboratory needs and facilities.
Differences of Vitamin D Levels in Controlled and Uncontrolled Type 2 Diabetes Mellitus Patients Adhisti Azlin; Ratna Akbari Ganie; Santi Syafril
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 1 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i1.1407

Abstract

Vitamin D as an immunomodulator, plays an important role in controlling glycemic levels and decreasing diabetes complications. HbA1c is a marker of glycemic control which is known to have association with vitamin D. This study aims to see the differences in vitamin D serum levels and glycemic index in patients with type 2 DM.The design of this study is cross-sectional study and it was performed in RSUP HAM in December 2017 until March 2018. DM type 2 patients were classified based on HbA1c <7% controlled and ≥ 7% uncontrolled. Vitamin D serum levels were examined using the Enzyme-Linked Fluourescent Assay (ELFA) method., differences in vitamin D levels among controlled and uncontrolled type 2 diabetes mellitus were assessed by using independent t-test, and the differences of HbA1c level were assessed using Mann-Whitney-U test.45 patients with type 2 DM were divided into controlled (HbA1c <7%) and uncontrolled patients (HbA1c ≥ 7%). There was no difference in vitamin D serum levels between controlled and uncontrolled DM Type 2 patients (p = 0,310).

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