Jaringan Laboratorium Medis
Jurnal Online Jaringan Laboratorium Medis adalah peer-reviewed journal yang mempublikasikan artikel-artikel ilmiah tentang ilmu kesehatan yang meliputi bidang Analis Kesehatan yang meliputi Penelitian Bakteriologi, Kimia Klinik, Parasitologi, Toksikologi Klinik, Hematologi, Sitohistoteknologi dan Imunologi, Teknologi Bank Darah. Dengan e-ISSN: 2685-8495 diterbitkan oleh Jurusan Analis Kesehatan Politeknik Kesehatan Kemenkes Semarang, terbit 6 bulan sekali, pada bulan Mei dan November setiap tahunnya.
Articles
262 Documents
Nilai Hematokrit pada Pasien Diare
Aulia Prameswari Hayuning Putri
Jaringan Laboratorium Medis Vol 3, No 2 (2021): November 2021
Publisher : Poltekkes Kemenkes Semarang
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DOI: 10.31983/jlm.v3i2.8064
Diarrhea is the passage of abnormal or watery stools that are more frequent than usual, and can be caused by infection or non-infection. Complications can occur including dehydration in patients with acute diarrhea. In cases of severe dehydration, hematological tests in diarrheal patients may be elevated, such as hematocrit and hemoglobin values. The purpose of this study was to determine the hematocrit value in inpatient diarrhea patients at the Tonjong Public Health Center, Brebes Regency. This type of research is a descriptive study using secondary data from medical record data as many as 30 respondents at the Tonjong Public Health Center, Brebes Regency. The results showed that there were 3 men who had decreased hematocrit levels and 8 people who had normal hematocrit levels. Whereas. on. In women, the hematocrit level decreased as much as. 17 people and 2 people who have normal hematocrit levels. Based on the age category, the most are the late elderly as many as 8 people and the final adults as much as 2 people. Meanwhile, based on the status of dehydration, all diarrhea patients who came for treatment at the Tonjong Health Center, Brebes Regency, 100% did not experience diarrhea with dehydration. The hematocrit levels in diarrhea patients at the Tonjong Public Health Center, Brebes Regency were 30 samples, on average they had low hematocrit levels.
Tingkat Pengetahuan terhadap Donor Darah pada Masyarakat Pendonor
Dina Afrianti;
Elis Susilawati;
Eko Naning Sofyanita
Jaringan Laboratorium Medis Vol 4, No 1 (2022): May 2022
Publisher : Poltekkes Kemenkes Semarang
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DOI: 10.31983/jlm.v4i1.8445
Blood donation is an activity to donate blood for transfusion purposes. The number of voluntary blood donors in Indonesia is around 20% of the total production of blood bags per year, the rest is met from replacement donors. People are generally reluctant to donate blood because of fear of pain when blood is drawn using a syringe, fear of running out of blood, resulting in anemia, contracting the disease through blood donors, feeling unwell to donate blood and feeling that it is enough to only donate blood once. This is a factor that causes the limited number of donors due to limited knowledge. Research Objectives: To see an overview of the level of knowledge about blood donors in the community of donors at Pekalongan City. Methods: The type of research used is descriptive quantitative with a cross sectional approach with a questionnaire to determine the level of knowledge of the donor community. Results: Most of the blood donors at the Pekalongan City are Male 73 respondents (73%), the highest percentage aged 17-30 years as many as 54 respondents (54%), high school education, namely as many as 49 respondents (49%). Most of the donor knowledge level is said to be good, namely 88 respondents (88%). Conclusion: Most of the donor community at the Pekalongan City have a good level of knowledge about blood donation.
Profil Mikroskopis Jaringan Hepar Mencit (Mus musculus) yang Difiksasi dengan Neutral Buffered Formalin (NBF 10%) dan Larutan Helly
Melly Agustin
Jaringan Laboratorium Medis Vol 3, No 2 (2021): November 2021
Publisher : Poltekkes Kemenkes Semarang
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DOI: 10.31983/jlm.v3i2.8053
The fixation solution that is widely used in anatomical pathology laboratories is NBF 10%, the excess of NBF 10% because the pH is close to neutral, can be stored in large quantities and a long time. Helly's fixation solution is a good fixation solution for the cytoplasm, and only requires 2-3 hours of fixation. Knowing the microscopic picture of the preparation of hepar mencit tissue (Mus musculus) fixated with NBF 10% and Helly solution. This research is an experimental study with a descriptive analysis approach. picture of hepar mencit tissue preparation (Mus musculus) fixation with NBF 10% obtained as much as 100% good preparation. While the fixated with Helly solution obtained as much as 66% good preparation. Conclusion: Microscopic picture of hepar mencit tissue preparation (Mus musculus) fixated with NBF is 10% better than Helly solution.
Profil Mikroskopis Jaringan Ginjal Mencit (Mus Musculus) yang Difiksasi dengan Madu Konsentrasi 10% Selama 24 Jam
Tasya Vita Brilian
Jaringan Laboratorium Medis Vol 3, No 2 (2021): November 2021
Publisher : Poltekkes Kemenkes Semarang
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DOI: 10.31983/jlm.v3i2.8066
Fixation is used to maintain tissue structure in its original form “life-like state” and can protect proteins and tissue components from degeneration. The solution commonly used is 10% NBF. Formaldehyde is chemical substance that is toxic and not environmentally friendly, several studies have shown alternative substitutes fixation, one of which is the honey solution. The study of Mohammed et al (2020) fixated tissue with honey 10% and 20% shown good coloring properties and similar clarity to fixated with formalin 10%. Honey has acidic and dehydrating properties allow most microorganisms to be killed so that tissues will last for a long time. The research objective is to findout the description of microscopic of mice (Mus musculus) kidney tissue which were fixation using 10% honey solution for 24 hours. The research is included in qualitative descriptive research. The research design used was a non-eksperimental with a purposive sampling study approach. The sample used was 32 preparation with total of microscopic overview is 160. Microscopic image of mice (Mus musculus) kidney tissue fixed using 10% honey solution for 24 hours in 80 visual fields were 12.5% of the preparations is not good and 87.5% is good preparations. The microscopic image of mice (Mus musculus) kidney tissue fixed using 10% NBF is better than of the microscopic image of mice (Mus musculus) kidney tissue fixed with 10% honey for 24 hours.
Rasio Penutupan Luka pada Tikus Diabetes Diinduksi Streptozotocin dengan Perlakuan Dressing Tipe Pasif dan Interaktif (Penelitian Pendahuluan)
Eko Naning Sofyanita;
Arya Iswara
Jaringan Laboratorium Medis Vol 3, No 2 (2021): November 2021
Publisher : Poltekkes Kemenkes Semarang
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DOI: 10.31983/jlm.v3i2.8045
Deparaffinization is a stage before the staining process to remove/dissolve paraffin so that the absorption of color in tissue preparations is maximized. Deparaffinization is usually carried out using xylol and toluol. Xylol has toxic effects including acute neurotoxicity, heart and kidney damage, hepatotoxicity, fatal blood dyscrasias, skin erythema, dry skin, peeling skin, and also has a carcinogenic effect. The toxicity effect of olive oil is lower than that of xylol. Oils that have non-polar properties can remove the remaining paraffin contained in the tissue. The purpose of this study was to determine the microscopic appearance of the kidney tissue preparations of mice deparaffinized with olive oil on hematoxylin eosin (HE) staining. The type of research used is experimental research which is analyzed with a descriptive approach. The results of the assessment of preparations deparaffinized with xylol in 80 visual fields obtained 100% good preparations and preparations deparaffinized with olive oil in 80 visual fields obtained 0% poor preparations, 11.3% poor preparations, and 88.7% good preparation. So it can be said that better results are found in the microscopic picture of the kidney preparations of mice (Mus musculus) deparaffinized with xylol.
Kadar Kadmium (Cd) dalam Urine pada Perokok Pasif
Nurhayana Nurhayana;
Anung Sugihantono
Jaringan Laboratorium Medis Vol 3, No 2 (2021): November 2021
Publisher : Poltekkes Kemenkes Semarang
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DOI: 10.31983/jlm.v3i2.8054
Smoking is a familiar activity in daily life. The existence of filters on cigarettes, causes the danger of cigarette smoke to be felt more by passive smokers. One of the content of cigarette smoke is cadmium (Cd) which is one of the environmental pollutants that are harmful and accumulate in the tissues of living things. The purpose of this study is to measure and describe the levels of Cadmium (Cd) in the urine of passive smokers in RT 03 RW 06 Gandekan Hamlet, Harjosari, Bawen District, Semarang Regency. Using observational (non-experimental) research design with descriptive research criteria. The number of samples of 12 urine from the community of RT 03 RW 06 Gandekan Hamlet, Harjosari, Bawen Subdistrict, Semarang Regency. The results of the examination of Cadmium Levels (Cd) on the 13 samples of passive smoker urine in gandekan RT 01 RW 06 Harjosari Subdistrict, Bawen Subdistrict, Semarang District received the highest level of 0.074 mg/L and the lowest result -0.005 mg/L. Differences in cadmium levels in the passive smoker urine were caused by several factors such as age, environment, health status, occupation, and consumption of antioxidants and milk. Cadmium (Cd) levels in the urine of all passive smokers were in the normal category (2mg/L).
Hubungan Antara Higiene Sanitasi Terhadap Kontaminasi Telur Cacing Non STH Pada Sayur Lalapan di Warung Makan
Lilik Setyowatiningsih;
Ririh Jatmi Wikandari;
Surati Surati
Jaringan Laboratorium Medis Vol 4, No 1 (2022): May 2022
Publisher : Poltekkes Kemenkes Semarang
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DOI: 10.31983/jlm.v4i1.8325
Fresh vegetables can be a source of transmission of worm infections to humans. The behavior of washing fresh vegetables that are not good by housewives and sellers of fresh vegetables in food stalls becomes large in transmitting worm infections if there are worm eggs that are still attached to fresh vegetables. The most common contamination found in fresh vegetables were eggs of the intestinal nematode Soil Transmitted Helminths (STH) as well as non-STH and trematodes. The purpose of this study was to determine the relationship between sanitation hygiene and contamination of non-STH worm eggs in fresh vegetables at food stalls in Semarang City. This type of research is an observational study with a cross sectional approach. Based on the results of laboratory tests, from a total of 165 samples examined, eggs of non-STH worms with species of Fasciola sp and Toxocara sp were found in 45 food stalls selling fresh vegetables. Based on bivariate analysis, there was no significant relationship between personal hygiene (p = 0.071) and environmental sanitation (p = 0.334) on contamination of non-STH worm eggs in fresh vegetables in food stalls in Pedurungan District, Semarang.
Kadar Hemoglobin pada Sampel Darah Petugas Stasiun Pengisian Bahan Bakar Umum (SPBU)
Rosalinda Zakhwa
Jaringan Laboratorium Medis Vol 3, No 2 (2021): November 2021
Publisher : Poltekkes Kemenkes Semarang
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DOI: 10.31983/jlm.v3i2.8063
Hemoglobin is a part of erythrocytes that serves to transport oxygen and carbon dioxide in tissues. Hemoglobin levels in the blood may decrease one of which is influenced by exposure to lead from the environment. Places that are at high risk for exposure to air lead one of them is at gas stations (Public Refueling Stations). The purpose of this study is to determine hemoglobin levels in kedungrandu gas station officers, Kec. Patikraja, Banyumas. This type of research is a non-experimental observational study. The method of measuring hemoglobin levels using strip test in the form of POCT (Point Care of Testing) tool. This method uses capillary blood samples from 20 respondents with criteria of male sex and aged 21-60 years. The result of this study was as many as 6 people (30%) of the total respondents had low hemoglobin levels, while 14 people (70%) have normal hemoglobin levels. The average hemoglobin level in the blood sample of kedungrandu gas station officers, Kec. Patikraja, Banyumas is 14.41 mg/dl or normal.
Kadar Asam Urat pada Perempuan Menopause
Safira Zahro;
Umi Rosidah
Jaringan Laboratorium Medis Vol 3, No 2 (2021): November 2021
Publisher : Poltekkes Kemenkes Semarang
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DOI: 10.31983/jlm.v3i2.8061
Based on data from Riskesdas 2013, in line with increasing age the prevalence of joint disease has increased. The trigger is abnormal metabolic processes in the body and 10% of cases occur in postmenopausal women because the production of the hormone estrogen is disrupted. If treatment is not carried out, it will result in chronic gouty arthritis, resulting in paralysis. The purpose of this study was to determine the description of uric acid levels in postmenopausal women in Berahan Kulon Village, Wedung District, Demak Regency. The research method used is an observational study with a cross sectional approach. Of the 20 research subjects, 1 research subject had low uric acid levels, 9 research subjects had normal uric acid levels, and 10 research subjects had high uric acid levels. High uric acid levels were dominated by the age group 51-55 years, menopause duration 6-10 years and 10 years, no history of heredity, and consumption of foods high in uric acid 3-5 times/week and 5 times/week. The conclusion of this study is that 5% of postmenopausal women have low uric acid levels, 45% have normal uric acid levels, and 50% have high uric acid levels.
Kadar Kreatinin Serum pada Pasien Rawat Inap yang Didiagnosis Diabetes Melitus Tipe 2
Tyas Pertiwi Armaningrum;
Teguh Budiharjo
Jaringan Laboratorium Medis Vol 4, No 1 (2022): May 2022
Publisher : Poltekkes Kemenkes Semarang
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DOI: 10.31983/jlm.v4i1.8352
Uncontrolled Diabetes Mellitus will lead to long-term complications, namely decreased organ function, especially the kidneys, nerves, eyes, blood vessels and heart. Diabetic nephropathy is a complication that is often found in DM patients and attacks the kidneys. Serum creatinine examination is used to assess kidney function in patients with Type 2 Diabetes Mellitus. This purpose of research is to describe the description of serum creatinine levels in inpatients diagnosed with Type 2 Diabetes Mellitus at Dr. Kariadi Semarang. This study uses descriptive quantitative. The data comes from the medical records of inpatients diagnosed with Type 2 DM at Dr. RSUP. Kariadi Semarang who examined creatinine levels in the period January - April 2020 with the total sampling method. From this study, inpatients diagnosed with Type 2 DM at Dr. Kariadi Semarang were 79 respondents with male sex as many as 49 people (62%) and women 30 people (38%). In 79 respondesnts, there are 2 respondents (2.5%) had low creatinine levels, 7 respondents (8.9%) had normal creatinine levels, while 70 respondents (91.1%) had high creatinine levels. 43 male patients (88.6%) were included in the category of high creatinine levels. Based on age, it was dominated by the late elderly (55 – 65 years) as many as 32 people (40.5%) with the late elderly patients as many as 28 people (87.5%) having high creatinine levels. Conclusion: In 79 respondesnts (100%), there are 72 respondents (91.1%) who have abnormal creatinine levels and 7 respondents (8.9%) have normal creatinine levels.