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Jaringan Laboratorium Medis
Published by Poltekkes Kemenkes Semarang
ISSN : -     EISSN : 26858495     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Public Health
Jurnal Online Jaringan Laboratorium Medis adalah peer-reviewed journal yang mempublikasikan artikel-artikel ilmiah tentang ilmu kesehatan yang meliputi bidang Analis Kesehatan yang meliputi Penelitian Bakteriologi, Kimia Klinik, Parasitologi, Toksikologi Klinik, Hematologi, Sitohistoteknologi dan Imunologi, Teknologi Bank Darah. Dengan e-ISSN: 2685-8495 diterbitkan oleh Jurusan Analis Kesehatan Politeknik Kesehatan Kemenkes Semarang, terbit 6 bulan sekali, pada bulan Mei dan November setiap tahunnya.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 270 Documents
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Pengaruh Stabilitas Sampel Darah EDTA Terhadap Pemeriksaan Hemoglobin dan Hematokrit pada Karyawan Puskesmas Kedung II Metode Otomatis Anjani, Anisa Ayu; Hartinah, Dewi; Kurnia, Shinta Dwi; Rusidah, Yunita; Saputro, Arief Adi
Jaringan Laboratorium Medis Vol. 7 No. 1 (2025): May 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i1.12872

Abstract

This study aims to determine the effect of EDTA blood sample stability on hemoglobin (Hb) and hematocrit (Ht) examination results using an automatic method. EDTA blood samples from 38 employees of Kedung II Public Health Center were examined immediately (0 hours), after a 1-hour delay at room temperature (17–210C), and after a 1-hour delay in a refrigerator (2–80C). The results were analyzed using a Two-Way ANOVA test. The study showed no significant differences in hemoglobin and hematocrit levels between the three storage conditions (ρ > 0.05). The average hemoglobin levels were 12.834 g/dL at 0 hours, 12.797 g/dL after 1 hour at room temperature, and 12.829 g/dL after 1 hour in the refrigerator. The average hematocrit levels were 38.484% at 0 hours, 38.321% after 1 hour at room temperature, and 38.434% after 1 hour in the refrigerator. The conclusion of this study is that storing EDTA blood samples for 1 hour, whether at room temperature or in the refrigerator, does not significantly affect hemoglobin and hematocrit examination results.
Hubungan Pengetahuan dengan Perilaku Ibu Hamil Usia Reproduksi Sehat dengan Pemeriksaan Triple Elliminatiom Skrining (HIV, Sifilis, dan Hepatitis B) Kahar, Fitriani
Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.12971

Abstract

Pregnant women are one of the populations at risk of contracting Sexually Transmitted Infections (STIs) such as HIV, Syphilis, and Hepatitis B. The prevalence of HIV/AIDS transmission from mother to child is 20-50% where 5-10% occurs during pregnancy. Syphilis transmission is 69-80% and Hepatitis B transmission is more than 90% with the potential for transmission during pregnancy and childbirth being very high. Based on the Regulation of the Minister of Health of the Republic of Indonesia, regulation No. 52 of 2017 concerning the Elimination Program for Transmission of Human Immunodeficiency Virus, Syphilis, and Hepatitis B from Mother to Child or often called "Triple Elimination" aims to stop transmission from mother to child with laboratory examinations on pregnant women at least once during pregnancy. The purpose of this study was to determine the relationship between knowledge and behavior of healthy reproductive age pregnant women in the first trimester at the Lebdosari Health Center. In general, this research method is qualitative with an analytical observational approach and cross-sectional design, namely researchers come and analyze the problem directly. Data collection is carried out at once within a certain time. The population is pregnant women in the first trimester at the Lebdosari Health Center, with a sample size of 49 people selected based on the Purposive Sampling technique. Data analysis was carried out descriptively and then continued with a bivariate test with the Kendal Tau test because the data was not normally distributed. The results of the study showed that the results of the bivariate test with the Kendal Tau test showed that there was no relationship between knowledge and behavior because the significance value p = 0.888> 0.05 with a positive correlation coefficient value (0.19) which means that there is a positive relationship between knowledge and behavior even though the relationship is weak because the value is less than 1, so that if knowledge increases, behavior will be better. It is recommended that pregnant women are advised to always maintain personal hygiene and implement a clean and healthy lifestyle to prevent infectious diseases.
Perbandingan Nilai Hematokrit Metode Mikrohematokrit dan Metode Automatik Hematology Analyzer Zybio Z3 Menggunakan Darah Vena di Klinik Utama Restu Ibu Jepara Asmawati, Dwi; Hartinah, Dewi; Saputro, Arief Adi
Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.12934

Abstract

Pregnant women are one of the populations at risk of contracting Sexually Transmitted Infections (STIs) such as HIV, Syphilis, and Hepatitis B. The prevalence of HIV/AIDS transmission from mother to child is 20-50% where 5-10% occurs during pregnancy. Syphilis transmission is 69-80% and Hepatitis B transmission is more than 90% with the potential for transmission during pregnancy and childbirth being very high. Based on the Regulation of the Minister of Health of the Republic of Indonesia, regulation No. 52 of 2017 concerning the Elimination Program for Transmission of Human Immunodeficiency Virus, Syphilis, and Hepatitis B from Mother to Child or often called "Triple Elimination" aims to stop transmission from mother to child with laboratory examinations on pregnant women at least once during pregnancy. The purpose of this study was to determine the relationship between knowledge and behavior of healthy reproductive age pregnant women in the first trimester at the Lebdosari Health Center. In general, this research method is qualitative with an analytical observational approach and cross-sectional design, namely researchers come and analyze the problem directly. Data collection is carried out at once within a certain time. The population  is pregnant women in the first trimester at the Lebdosari Health Center, with a sample size of 49 people selected based on the Purposive Sampling technique. Data analysis was carried out descriptively and then continued with a bivariate test with the Kendal Tau test because the data was not normally distributed. The results of the study showed that the results of the bivariate test with the Kendal Tau test showed that there was no relationship between knowledge and behavior because the significance value p = 0.888> 0.05 with a positive correlation coefficient value (0.19) which means that there is a positive relationship between knowledge and behavior even though the relationship is weak because the value is less than 1, so that if knowledge increases, behavior will be better. It is recommended that pregnant women are advised to always maintain personal hygiene and implement a clean and healthy lifestyle to prevent infectious diseases.
Efektivitas Masa Penyimpanan Filtrat Daun Jati (Tectona grandis) terhadap Kualitas Pewarna Preparat Telur STH (Soil Transmitted Helminth) Rahmadhani, Afiffah Gita; Afrianti, Dina; Priyatno, Djoko
Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13018

Abstract

Soil-Transmitted Helminth (STH) infections such as Ascaris lumbricoides remain a public health concern in Indonesia, necessitating microscopic fecal examinations using high-quality stains. Eosin is commonly used but poses environmental and health risks. Anthocyanins found in teak leaf (Tectona grandis) filtrate offer a safer and more eco-friendly alternative. One factor affecting anthocyanin color stability is storage duration. This study aimed to evaluate the effectiveness of teak leaf filtrate stored for 0, 7, 14, and 21 days on the staining quality of STH egg preparations. This quasi-experimental study used a Posttest-Only Nonequivalent Control Group Design. The samples consisted of 24 Ascaris lumbricoides egg preparations divided into four treatment groups with six replications each. Staining quality was assessed by three validators based on contrast under the microscope, color absorption, and morphological clarity. Data were analyzed using the Kruskal-Wallis test to determine differences in staining quality across storage durations. All preparations showed good staining quality, and no significant differences were found between the storage groups (p-value = 0.383; > 0.05). These results indicate that teak leaf filtrate remains effective for staining STH egg preparations for  up to 21 days of storage, with the best results observed at 7 days after preparation. Further research is recommended to explore this topic more deeply, with attention to laboratory procedures and technical skills.
Hubungan Indeks Massa Tubuh dengan Kadar Glukosa Darah Sewaktu pada Mahasiswa Teknologi Laboratorium Medis Program Diploma Tiga STIKES Wira Medika Bali Anjaswari, Ni Made Dian; Abadi, Moh. Fairuz; Prasetya, Didik
Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13197

Abstract

Body Mass Index (BMI) is a simple parameter used to assess a person's nutritional status, whether they are underweight or overweight. Overweight is often associated with the risk of elevated blood glucose levels (hyperglycemia), which can develop into metabolic syndrome. This condition is increasingly found in adolescents and young adults with unhealthy lifestyles. Therefore, this study was conducted to determine the relationship between BMI and random blood glucose levels (RBG) in regular students of the Diploma III Medical Laboratory Technology program at Wira Medika Bali Health College. This study employed an observational analytical method with a cross-sectional approach. A sample of 30 students was selected through purposive sampling based on inclusion criteria: aged 15–24 years, not taking medications that affect blood sugar levels, and willing to participate. BMI data were obtained through weight and height measurements, then categorized into underweight, normal weight, overweight, obesity I, and obesity II. RBG examinations were performed using capillary blood with the aid of a glucometer. Analysis of the relationship between variables used the Spearman Rank Test. The results showed that the BMI distribution of respondents was 13.3% underweight, 40.0% normal weight, 13.3% overweight, 23.4% obesity I, and 10.0% obesity II. All respondents had random blood glucose levels <200 mg/dL, thus remaining within the normal range. Statistical testing showed a p-value of 0.996 (p>0.05), concluding that there was no significant relationship between BMI and GDS in students. The conclusion of this study is that BMI has not been proven to be directly related to random blood glucose levels. However, weight control remains necessary as a long-term preventive measure against metabolic syndrome and other non-communicable diseases associated with obesity.
Gambaran Hasil Pemeriksaan Telur Cacing Soil Transmitted Helminths dari Sediaan Feses dan Kuku dengan Metode Flotasi pada Pengangkut Sampah di TPS 3R Padangsambian Erikayani, Ni Made Dwi; Idayani, Sri; Bintari, Ni Wayan Desi
Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13207

Abstract

Helminthiasis is a disease caused by parasitic worms that infect the human body. Transmission can occur through contaminated food and water, as well as through skin penetration via soil as the intermediate medium. Soil-Transmitted Helminths (STH) are a group of parasitic worms transmitted through soil and can cause infections, particularly among populatihons with poor hygiene, including waste collectors. This study aims to describe the findings of STH egg examinations from fecal and nail samples using the flotation method among waste collectors. Samples were collected using purposive sampling from waste collectors at TPS 3R Padangsambian based on inclusion and exclusion criteria. This descriptive study employed a qualitative approach. Data analysis was conducted descriptively, and the examination results were presented in tables and narratively explained using univariate analysis to illustrate the frequency and percentage distribution of each research variable. A total of 11 respondents participated in the examination. Fecal and nail specimens were analyzed using the flotation method at the Parasitology and Mycology Laboratory of STIKES Wira Medika Bali. The results showed that 6 fecal samples (54.6%) were positive for STH eggs, while 5 samples (45.5%) were negative. In nail samples, 1 sample (9.1%) tested positive and 10 samples (90.9%) were negative. The identified worm species included Ascaris lumbricoides and Trichuris trichiura. Examination of fecal samples was found to be more effective than nail samples; however, nail examination can serve as an additional detection method for individuals at high risk of STH exposure.
Fluktuasi Aktivitas Enzim SGOT dan SGPT pada Pasien Kanker Payudara yang Menjalani Kemoterapi di RSUD Provinsi NTB Ananda, Fadila Rizki; Tatontos, Erlin Yustin; Fihiruddin, Fihiruddin
Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13322

Abstract

Chemotherapy is one of the primary treatment for breast cancer. Despite its effectiveness, chemotherapy has the potential to cause hepatotoxicity due to drug metabolism in the liver. This condition is generally indicated by elevated levels of SGOT and SGPT enzymes. Liver enzyme monitoring is essential to assess hepatocellular function throughout the course of therapy. This research aims to determine fluctuations in SGOT and SGPT enzyme activity in breast cancer patient undergoing chemotherapy at the NTB Provincial Hospital, and describe the pattern of changes in enzyme values based on the frequency of chemotherapy cycles. An analytical observational study with a cross-sectional approach was employed. Samples were selected purposively based on the medical records of breast cancer patients who underwent 1 to 6 cycles of chemotherapy between January and December 2024. Data were analyzed descriptively based on the distribution and the average of SGOT and SGPT enzyme levels. The results showed fluctuating SGOT and SGPT levels across different chemotherapy cycles. There was no consistent pattern of increase in enzyme activity based on chemotherapy frequency.  The average SGOT levels from cycle 1 to 6 were 24, 28, 27, 30, 33, and 24 IU/L, while the average SGPT levels were 35, 27, 29, 25, 29, and 29 IU/L, with a range of SGOT values of 13-404 IU/L and SGPT with a range of 5-304 IU/L. most enzyme values remained within normal range. It was concluded that chemotherapy frequency did not show a consistent association with increased SGOT and SGPT activity. These research emphasize the importance of routine monitoring of liver function during each chemotherapy cycle, so that potential hepatotoxicity can be adjusted according to the patient condition.  
Sensitivitas dan Spesifisitas Pemeriksaan Antibodi Human Immunodeficiency Virus (HIV) dengan Perbedaan Metode Imunokromatografi Rapid Test terhadap ECLIA (Electro Chemiluminescence Immuno Assay) Aini, Resmi; Padmarini, Adira; Sulistyo, Andhy
Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13620

Abstract

The requirement for HIV reagents using the Immunochromatographic Rapid Test method must comply with the WHO prequalification standards and evaluation by the National Evaluator Agency to ensure their quality and safety; therefore, their sensitivity and specificity were tested against the ECLIA method as the gold standard. To determine the sensitivity and specificity of HIV testing using the Immunochromatographic Rapid Test compared with ECLIA at UDD PMI Yogyakarta City. The study population consisted of all blood donor samples undergoing screening for transfusion-transmissible infections (TTI). Sampling was conducted using the Systematic Random Sampling technique, by determining a random starting point with a Random Number Generator and subsequently selecting samples at fixed intervals until 30 samples were obtained. These samples were then tested using both ECLIA and the Rapid Test. The study design was observational analytic with a cross-sectional approach to evaluate the correlation between Rapid Test results and the ECLIA method as the gold standard, and to calculate sensitivity, specificity, PPV, and NPV accurately. A total of 30 blood donor samples were examined. Based on the ECLIA results, 4 samples were identified as HIV-reactive and 26 samples were non-reactive. The Immunochromatographic Rapid Test detected only 2 of the 4 ECLIA-confirmed reactive cases. The test showed high specificity (100%) but relatively low sensitivity (50%). ECLIA detected 4 (13.3%) reactive and 26 (86.3%) non-reactive samples. The sensitivity of the Immunochromatographic Rapid Test compared to ECLIA was 50%, while the specificity was 100%. These results indicate that the Rapid Test does not meet the requirements stated in the 2018 CPOB guidelines, particularly regarding HIV screening, which requires a specificity of more than 98%.
Perbadingan Hasil Pemeriksaan pH dan Leukosit Menggunakan Urin Segar dan Urin Tunda 2 Jam pada Pasien Infeksi Saluran Kemih (ISK) dengan Metode Carik Celup Natalia, Anjela Stefanie; Abadi, Moh. Fairuz; Subhaktiyasa, Putu Gede
Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13196

Abstract

Urinary Tract Infection (UTI) is one of the most prevalent diseases that requires rapid and accurate laboratory diagnosis. Urine examination using dipstick methods serves as a practical diagnostic alternative. However, the stability of urine specimens subjected to delayed examination needs further evaluation, particularly regarding pH and leukocyte parameters. This study aimed to determine the differences in urine pH and leukocyte results between fresh urine and urine stored for 2 hours at room temperature using the dipstick method in UTI patients. This experimental study employed a quantitative approach. A total of 30 urine samples were collected from UTI patients at RSAD Tk. II Udayana. Each sample was analyzed under two conditions: immediately (fresh urine) and after 2 hours of storage at room temperature (delayed urine), yielding a total of 60 measurements. The results showed an increase in the median urine pH from 6.0 to 6.5 and a decrease in leukocyte count from a median of 125 to 70 after 2 hours of storage at room temperature, with statistically significant differences (p < 0.05). These findings indicate that delaying urine examination for 2 hours can affect pH and leukocyte values, potentially leading to inaccurate diagnostic interpretation. Therefore, urine chemical examination should be performed within one hour after collection or stored at 2–8 °C to maintain result accuracy.
Pengaruh Penundaan Waktu Pemeriksaan Terhadap Kadar Hemoglobin Metode Kolorimetri Carolina, Ega; Widiyanto, S.Y Didik; Anung Sugihanto
Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13481

Abstract

The workload of laboratory staff at the Community Health Center (Puskesmas) is disproportionate to the number of specimens examined, and laboratory equipment is limited. Hemoglobin (Hb) testing is a laboratory test frequently used to support disease diagnosis. Other causes of delays include electrical instability, equipment failure, delayed specimen delivery, shift changes, equipment failure, and excessive blood draws from patients (Ma, Aryandi & Novriani, 2019). This study aimed to determine the effect of the time required for hemoglobin testing using the direct colorimetric method, a 90-minute delay, and a 180-minute delay. This study was a pure experiment using a completely randomized design. The sample consisted of 48 patients, all patients referred to the Plamongan Sari Community Health Center laboratory for hemoglobin testing. The sample had completed informed consent for blood draws. Data analysis included a normality test, which showed a normal distribution, followed by a homogeneity test, which showed a homogeneous data distribution. The results were then analyzed using a one-way ANOVA test at α = 0.05. The study showed no significant difference between hemoglobin levels measured immediately and those delayed by 90 minutes and 180 minutes using the colorimetric method (p-value 0.540). Hemoglobin levels can still be measured after a 90-minute and 180-minute delay. However, it is recommended that samples referred for hemoglobin testing be tested immediately without delay.

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