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Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)
Published by Universitas Lampung
ISSN : 23384344     EISSN : 2686200X     DOI : https://doi.org/10.23960/jbekh.vxxx.xxx
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
This journal focuses on the fields of Agricultural, Animal Sciences, Bioconservation, Biopharmacology, Biotechnology, Biomedical, Biological control, Behavioural ecology, Plant Sciences, Ecology, Fishery, Marine Biology, Fresh Water Biology, Microbiology, Immunobiology, Ecotoxicology, and Parasitology. This journal utilizes both the LOCKSS and CLOCKSS systems to create a distributed archiving system among participating libraries and permits those libraries to create permanent archives of the journal for purposes of preservation and restoration
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 223 Documents
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BIOACCUMULATION OF IRON (Fe) IN Bacillus JA1, Sporosarcina JA4, AND Lysinibacillus JB2 Afianita Dian Rahmawati; Enny Zulaika
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.188

Abstract

Carbonatogenic bacteria are able to produce calcium carbonate (CaCO3). Steel reinforcement of concrete is mainly made of a mixture of carbon (C) and ferrous (Fe) elements. Fe is classified as heavy metal, when in high concentrations it is toxic to bacteria. The purpose of this study was to determine the bioaccumulation of carbonatogenic bacteria to Fe. The isolates used in this study were Bacillus JA1, Sporosarcina JA4, and Lysinibacillus JB2. All isolates were subcultured on nutrient agar slant media. Cultures were made on minimal salt medium: nutrient broth (95% : 5%) with 24 hours incubation. Bioaccumulation test using minimal salt medium: nutrient broth (95% : 5%) containing ferrous 5 ppm and 10 ppm. Accumulated ferrous concentration was measured using atomic absorption spectrophotometry. The result of this research is that the bioaccumulation of ferrous by the isolates after 2 hours of incubation is greater than 1 hour of incubation. Bacillus JA1 has the highest bioaccumulation ability which was 9,19 ppm after 2 hours exposure of 10 ppm ferrous and its ferrous bioaccumulation efficiency was 91,85%.
THE RESISTANCE OF CHILLI (Capsicum annuum L.) FROM SEEDS INDUCED BY 0.2 mT MAGNETIC FIELD AND INFECTED BY Fusarium oxysporum Feni Kaisah; Rochmah Agustrina; Eti Ernawiati; Martha L. Lande; Lili Chrisnawati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.191

Abstract

Red chili pepper (Capsicum annuum L.) is one of the most widely cultivated commodities in Indonesia. However, until now the production was never covering the public’s demand. Red chili pepper plants are susceptible to disease caused by the infection of Fusarium oxyporum (Fox) fungi, causing a decrease in plants’ production. Fungicide usage to control Fox can cause plants’ resistance to the pathogen. The eco-friendly way to control Fox could be achieved by utilizing a magnetic field. This research arranged using Completely Randomized Design (CRD) with the combination between 0,2 mT magnetic field induction and Fox infection consist of control (M0); 0,2 mT magnetic field induction for 7 minutes and 48 seconds (M7); and 15 minutes and 36 minutes (M15); as well as seed infection with Fusarium oxyporum that consist of control without infection (F0) and infected for 60 minutes (F60). Each test unit was repeated 5 times.   
EVALUATION OF WATER QUALITY IN ANIMAL FEED FACTORY ENVIRONMENT BASED ON PLANKTON AND BENTHOS BIOINDICATORS Feni Yulinda; Tugiyono; Agus Setiawan; Elly Lestari Rustiati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.192

Abstract

Water is a source of life that plays an important role for all living things. Rivers are one of the water sources that are vulnerable to pollution. This study aimed to evaluate the water quality of the Way Napal river based on the diversity of plankton and benthos. The research was conducted from November 2020 to March 2021. Sampling and analysis were performed by field technicians from the SEAMEO BIOTROP Environmental Laboratory. Plankton diversity was calculated using the Shannon Weaver diversity index. The results showed that the phytoplankton diversity index at AP-1, AP-2, and AP-3 locations ranged between 2,080-2,278 these values had moderate biota stability and were included in the uncontaminated category. The zooplankton diversity index at the AP-1 location has a value of 1,119 with moderate biota stability and is included in the moderate category, while at the AP-2 and AP-3 locations it has a value of 0.925-0.995 with unstable biota diversity and is included in the highly polluted category. The Benthos diversity index ranges from 1.343 - 1.772 with moderate biota stability and is included in the moderately polluted category.
THE DYNAMICS OF BRYOPHYTES SPECIES DIVERSITY IN THE LOWLAND ECOSYSTEMS, CIBINONG SCIENCE CENTER-BOTANICAL GARDEN Fandri Sofiana Fastanti; Tri Yuni Indah Wulansari
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.194

Abstract

Bryophytes are a group of plants generally with small sizes and spores as generative reproduction. There are three main groups of Bryophytes: liverworts or hepatics (Marchantiophyta), mosses (Bryophyta), and hornworts (Anthocerophyta). They are found in almost all ecosystems. Ecopark Cibinong Science Center-Botanical Garden (CSC-BG) is a lowland ecosystem protection area located in industrial cities. The bryophytes species that were carried out in 2009 and 2010 are inadequate. Re-exploration activity was carried out from June 2018 until January 2019 in all blocks of Ecopark. This study aims to report an update of bryophytes data in Ecopark. A total of 20 species of bryophytes were found in this research. Fissidens is the most common genus of bryophytes. There are 11 species of bryophytes and 9 species are new data records for Ecopark CSC-BG. This study updated bryophyte diversity data from Ecopark CSC-BG in 2009 and 2010, resulting in the discovery of 30 species (27 species mosses, 3 species liverworts), including 10 species not found in previous studies. There are no hornworts found in Ecopark CSC-BG.
THE ARACEAE OF ITB JATINANGOR CAMPUS, SUMEDANG, WEST JAVA Arifin Surya Dwipa Irsyam; Reza Raihandhany; Muhammad Rifqi Hariri; Rina Ratnasih Irwanto
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.198

Abstract

Araceae is a group of plants that are consistently dominant in humid and wet conditions areas. This family is among the most common plant groups found in the campus Institut Teknologi Bandung (ITB) at Jatinangor, which are still covered by green areas. However, the diversity of Araceae has not been revealed yet. Therefore, this research was conducted to provide information on the Araceae in ITB Jatinangor Campus. Field exploration was carried out during September - November 2021 at ITB Jatinangor. As many as 15 genera and 22 species of Araceae have been discovered within the campus. Taxonomically, they are grouped into 3 subfamilies, i.e Aroideae (12 genera), Monsteroideae (2 genera), and Pothoideae (1 genus). The largest genera of Araceae found on the campus were Anthurium and Philodendron, both cultivated as ornamental plants. Several local species, such as Alocasia macrorrhizos, Colocasia esculenta, Pistia stratiotes, Typhonium flagelliforme, and T. roxburghii were also found. Three other ornamental species have been escaped from cultivation, namely Caladium bicolor, Dieffenbachia seguine, and Syngonium podophyllum had were also found here. This research was part of the Flora Campus ITB Jatinangor Exploration, a project initiated by Herbarium Bandungense.
INDUCTION OF POLYPLOID BANANA KEPOK THROUGH IN VITRO ADDITION OF FLAME LILY EXTRACT Eti Ernawiati; Lili Chrisnawati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.201

Abstract

Flame lily (Gloriosa superba L.) contains colchicine and gloriosin in almost all parts of the plant, especially in tubers and seeds. Colchicine is often used in biological and breeding studies to induce mutations that result in polyploid plants. This study aimed to examine the effectiveness of flame lily tuber extract as a natural mutagen for the development of superior kepok banana cultivars through the in vitro assembly of polyploid kapok bananas. The study was arranged completely randomly with two factors. Factor 1 consisted of 3 levels, namely fresh extract of breech flower tubers (10 %), pure colchicine solution (0.1%) as a positive control, and without being added (0%) as a negative control. Factor 2 consists of 3 levels, namely Kepok Abu, Kepok Batu and Kepok Kuning. All treatment combinations were repeated 5 times. The data were analyzed by Diversity Test (Sidik Ragam) and if there is a difference, it will be continued with the DMRT test at a level of 5%. The results showed that the administration of colchicine in culture media could induce the emergence of polyploid banana plantlets when viewed from the addition of the size of the number of shoots, shoot length, number of roots, root length, leaf area, epidermal cells, stomata, and decreased stomata index. Meanwhile, 10% breech flower tuber extract was not able to induce the emergence of polyploid plantlets. Kepok Kuning bananas had a better response to mutagens than Kepok Abu and Kepok Batu. From these results, it can be concluded that 0.1% colchicine was able to induce polyploid banana plantlets and 10% breech flower tuber extract was not able to induce polyploid plantlets.
The Ability of Soil Bacteria from Liwa Botanical Gardens to Produce Indole Acetic Acid Hormone (AIA) Ekowati, Cristina Nugroho; Sanjaya, Agung; Umar, Suratman; Sumardi, Sumardi
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 1 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i1.202

Abstract

Hormon AIA berperan besar dalam memperbesar dan memperpanjang sel, pembelahan sel, khususnya pada daerah ujung tanaman. Bakteri yang memiliki kemampuan dalam menghasilkan hormon AIA diantaranya  Enterobacter sp., Azospirillum sp., Klebsiella sp., Alcaligenes faecalis, Azoarcus sp., Serratia sp., Azotobacter sp., Cyanobacteria, Erwinia herbicola, Pseudomonas sp., Rhizobium, Bradyrhizobium, Agrobacterium tumafaciens. Sintesis AIA ini memerlukan senyawa tambahan berupa triptopan. Namun beberapa bakteri mampu memproduksi AIA tanpa penambahan prekursor. Penelitian ini bertujuan untuk memperoleh isolat bakteri penghasil hormon AIA dari tanah Kebun Raya Liwa. Pada ini diawali dengan isolasi bakteri tanah asal Kebun Raya Liwa, selanjutnya dilakukan uji kemampuan produksi AIA secara kuantitatif tanpa penambahan triptopan. Pengujian produksi AIA secara kualitatif dilakukan dengan menggunakan media Nutrien Broth dengan metode spektrofotometri dengan penambahan reagen salkowsky dan dilakukan pengamatan setelah 72 jam inkubasi. Data yang didapatkan disajikan dalam bentuk tabel. Hasil penelitian didapatkan 9 isolat yang mampu menghasilkan hormon AIA dengan kadar yang berbeda. Isolat penghasil AIA tertinggi yaitu isolat DT1 dengan kadar 114 ppm yang mempunyai karakteristik berbentuk bacil berwarna putih susu dan tidak berspora.     AIA hormone plays a role in enlarging and elongating cells, and cell division, especially at the tip of the plant. Bacteria that have the ability to produce IAA hormones include Enterobacter sp., Azospirillum sp., Klebsiella sp., Alcaligenes faecalis, Azoarcus sp., Serratia sp., Azotobacter sp., Cyanobacteria, Erwinia herbicola, Pseudomonas sp., Rhizobium, Bradyrhizobium, Agrobacterium tumafaciens. The synthesis of AIA requires an additional compound in the form of tryptophan. However, some bacteria can produce AIA without the addition of precursors. The goal of this study was to obtain isolates of AIA hormone-producing bacteria from the soil of the Liwa Botanical Gardens. This begins with the isolation of soil bacteria from the Liwa Botanical Gardens, then a quantitative test of AIA production capability is carried out without the addition of tryptophan. Qualitative testing of AIA production was carried out using Nutrien Broth media with spectrophotometric methods with the addition of Salkowsky reagent and observations were made after 72 hours of incubation. The data obtained are presented in tabular form. The results showed that 9 isolates were able to produce the AIA hormone at different levels. The highest AIA-producing isolate was the DT1 isolate with a concentration of 114 ppm which had the characteristics of a milky white bacillus and no spores.      
The Potential of Soil Bacterial Isolates from Liwa Botanical Gardens, West Lampung as Phosphate Solubilizing Bacteria Ekowati, Cristina Nugroho; Shintia, Rina; Umar, Suratman; Irawan, Bambang
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 1 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i1.203

Abstract

Tanah Kebun Raya Liwa (KRL) Lampung Barat merupakan tanah jenis laterit yang kandungan unsur hara dan kesuburan tanahnya rendah, sehingga tidak dapat mendukung pertumbuhan tanaman hias yang ada secara optimal. Salah satu unsur hara yang penting dalam menunjang kesuburan dan pertumbuhan tanaman adalah fosfat. Jumlah fosfat terlarut dalam tanah yang dapat dimanfaatkan oleh tanaman sangat sedikit sehingga menyebabkan defisiensi. Bakteri pelarut fosfat asli dibutuhkan untuk memenuhi kebutuhan fosfat bagi tanaman. Tujuan dari penelitian ini adalah untuk mendapatkan isolat bakteri pelarut fosfat yang berpotensi melarutkan fosfat. Penelitian ini dilaksanakan pada bulan Maret sampai Juni 2021 meliputi isolasi bakteri pelarut fosfat dan pengujian potensi pelarutan fosfat pada media pikovskaya padat. Potensi bakteri pelarut fosfat ditentukan berdasarkan nilai Indeks Kelarutan Fosfat (IPF) tertinggi. Hasil isolasi tanah dari Kebun Raya Liwa diperoleh 10 isolat yang mampu melarutkan fosfat dan menghasilkan zona bening. Isolat dengan potensi pelarutan fosfat terbaik adalah isolat C1 dan C3 dengan nilai indeks pelarutan fosfat 2, 6 dan 2.       Liwa Botanical Gardens (KRL) soil, West Lampung is a lateritic soil type nutrient content and low soil fertility, so it can’t support the growth of existing ornamental plants optimally. One of the important nutrients in supporting fertility and plant growth is phosphate. The amount of dissolved phosphate in the soil that can be utilized by plants is very small, causing a deficiency. Indigenous phosphate solubilizing bacteria are needed to meet the needs of phosphate for plants. The purpose of this study was to obtain isolates of phosphate solubilizing bacteria that have the potential to dissolve phosphate. This research was carried out from March to June 2021 covering the isolation of phosphate solubilizing bacteria and testing the potential for dissolving phosphate on solid pikovskaya media. Potential phosphate solubilizing bacteria were determined based on the highest Phosphate Solubility Index (IPF) value. The results of soil isolation from Liwa Botanical Gardens obtained 10 isolates capable of dissolving phosphate and producing a clear zone. Isolates with the best phosphate solubilizing potential were isolates C1 and C3 with phosphate solubilizing index values of 2, 6 and 2.      
DETECTION OF NITROGENASE PRODUCING BACTERIA FROM THE SOIL OF LIWA BOTANICAL GARDEN Cristina Nugroho Ekowati; Mica Mirani; Kusuma Handayani; Rochmah Agustrina
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.204

Abstract

Liwa Botanical Gardens is an ex-situ conservation area for various types of plants. Each plant produces organic matter that will provide nutrients for the growth of nitrogen-fixing bacteria. This indicates the existence of an environment that supports the growth of nitrogen-fixing bacteria. Nitrogen is one of the nutrients needed by plants for their growth. However, the abundance of nitrogen in the atmosphere cannot be utilized directly by plants but needs to transform into ammonium and nitrate first. This transformation can be done by nitrogen-fixing bacteria through an enzymatic process. This research aims to obtain bacterial isolates that can fix nitrogen. Nitrogen-fixing bacteria were isolated using Nutrient Agar (NA) medium and furthered by nitrogenase activity detection test with semi-solid Nitrogen Free Bromothymol Blue (NFB). Nitrogen-fixing bacteria are characterized by color changes in the medium. The results obtained 22 isolates with 3 isolates detected capable of producing nitrogenase enzymes, namely TBP B3, TB1 B2, and TMA2 B2.
Effectiveness of Ovitrap Against Aedes aegypti Mosquito In Kemiling Raya Sub-District Bandar Lampung City And The Vulnerability Of Its Larvals To Temephos Indriyani, Iin; Rosa, Emantis; Dania Pratami, Gina; Nukmal, Nismah
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 1 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i1.205

Abstract

Demam Berdarah Dengue (DBD) adalah salah satu masalah kesehatan masyarakat, yang disebabkan oleh Virus Dengue. Di Indonesia kasus DBD masih tinggi, khususnya di Lampung, salah satu Kota dengan angka kasus DBD yang masih tinggi adalah Bandar Lampung. Dari berbagai hasil penelitian diketahui ovitrap memiliki fungsi monitoring serta pengendalian Aedes sp.  Upaya pengendalian vektor nyamuk Ae. aegypti banyak dilakukan baik secara alami ataupun dengan cara kimia, cara kimia yang paling umum digunakan adalah penggunaan temephos. Penggunaan insektisida kimia seperti temephos secara terus menerus dapat menyebabkan resistensi. Tujuan penelitian untuk mengetahui efektivitas ovitrap di dalam dan luar rumah, mengetahui ovitrap index, serta status kerentanan larva nyamuk Ae. aegypti terhadap temephos di Kelurahan Kemiling Raya Kota Bandar Lampung. Penelitian dilakukan bulan Februari-April 2021. Ovitrap diletakkan di 142 rumah di dalam dan luar rumah, hasil telur dihitung, dipelihara dan diuji kerentananya. Bahan yang digunakan larva instar III hasil pemeliharaan hingga F1. Penelitian dilakukan dengan 4 kali ulangan, menggunakan 25 larva pada tiap gelas dengan larutan temephos 0,02 mg/L dalam 250 mL air dibiarkan kontak uji selama 1 jam dan masa pemulihan selama 24 jam. Hasil yang di dapat 83,10% rumah terdapat telur pada ovitrap dan 16,90% tidak ada telur, jumlah telur pada ovitrap di luar rumah (57%) lebih banyak dari dalam rumah (43%) serta ovitrap index yang didapatkan adalah 72,5%, persentase kematian 99% dan dikategorikan rentan.     Dengue Hemorrhagic Fever is one of the public health problems, which is caused by the Dengue Virus. In Indonesia, dengue cases are still high, especially in Lampung, one of the cities with a high number of dengue cases is Bandar Lampung. From various research results, it is known thatovitrap has the function of monitoring and controlling Aedes sp. Efforts to control the mosquito vector of Ae. aegypti is mostly done either naturally or chemically, the most commonly used chemical method is the use of temephos. The continuous use of chemical insecticides such as temephos can cause resistance. The purpose of the study was to determine the effectiveness of ovitrapinside and outside the home, to determine the ovitrap index, and the susceptibility status of Ae. aegypti against temephos in Kemiling Raya Village, Bandar Lampung City. The study was conducted in February-April 2021. Ovitrapswere placed in 142 houses inside and outside the house, egg yields were counted, maintained, and tested for susceptibility. Materials used for larvae instar III reared up to F1. The study was carried out with 4 replications, using 25 larvae in each glass with a solution of temephos 0.02 mg/L in 250 mL of water and allowed to contact the test for 1 hour and the recovery period for 24 hours. The results obtained were 83.10% of the house contained eggs in the ovitrap and 16.90% had no eggs, the number of eggs in the ovitrap outside the home (57%) was more than inside the house (43%) and the ovitrap index obtained was 72, 5%, the percentage of deaths is 99% and is categorized as vulnerable.    

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