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Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)
Published by Universitas Lampung
ISSN : 23384344     EISSN : 2686200X     DOI : https://doi.org/10.23960/jbekh.vxxx.xxx
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
This journal focuses on the fields of Agricultural, Animal Sciences, Bioconservation, Biopharmacology, Biotechnology, Biomedical, Biological control, Behavioural ecology, Plant Sciences, Ecology, Fishery, Marine Biology, Fresh Water Biology, Microbiology, Immunobiology, Ecotoxicology, and Parasitology. This journal utilizes both the LOCKSS and CLOCKSS systems to create a distributed archiving system among participating libraries and permits those libraries to create permanent archives of the journal for purposes of preservation and restoration
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 223 Documents
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VEGETATIVE GROWTH OF TOMATO (Lycopersicum esculentum Mill.) INFLUENCED BY AERATED COMPOST TEA (ACT) FROM BROMELAIN LITTER INDUCED BY LIGNINOLITIC Trichoderma sp. Sela Habibu Rohmah; Bambang Irawan; Salman Farisi; Yulianty
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.164

Abstract

The pineapple waste increased by the rise of production in pineapple, one of increasing pineapple waste is pineapple pulp (bromelain litter). The bromelain litter will be disadvantage for the environment, this matter could be solved by converting bromelain litter into compost. The composting process in this study used ligninolytic fungus (Trichoderma sp.). Composting technology which currently has rapidly developed is Aerated Compost Tea (ACT) or a derivate product of compost. The use of this study was to ensure the best incubation period of ACT bromelain litter which induced by Trichoderma sp. fungus on the growth of tomato (L. esculentum Mill.). The study was conducted using a completely randomized design (CRD) with 7 treatments and 3 replications, namely: P0= control (without ACT), P1= ACT bromelain 24 hours, P2= ACT bromelain 48 hours, P3= ACT bromelain 72 hours, P4= ACT bromelain and leaf litter 24 hours, P5= ACT bromelain and leaf litter 48 hours, and P6= ACT bromelain and leaf litter 72 hours. The variables observed were number of leaves and plant chlorophyll content. The data obtained were analyzed with ANOVA at the level of 5 % and tested for the Least Significant Difference (LSD) at the level of 5 %. The results of this study showed that ACT was induced by Trichoderma sp. fungus which effective for the vegetative growth of tomato (L. esculentum Mill.) is ACT bromelain litter with incubation period up to 72 hours (P3).
BROMELAIN WASTE TEA COMPOST INDUCED BY LIGNINOLYTIC INOCULUM OF Trichoderma sp. ON THE GROWTH OF LEAF NUMBER AND CHLOROPHYLL CONTENT OF CHILI (Capsicum annuum L.) Syafira Clarisa Huda; Bambang Irawan; Salman Farisi; Yulianty Yulianty
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.165

Abstract

Pineapple is one kind of fruit that contains a lot of nutrition.  The waste from residual pineapple processing is called bromelain.  Bromelain contains polymer complex in the form of organic sources from nature if it could be decomposed properly.  One way to exploited bromelain is by composting.  Composting is done with the help of fungi of Trichoderma sp. to speed up the decomposition process.  The resulting compost is aerated to increase the nutrients and form a further decomposition process in the soil.  The research was carried out from January till March 2021 in the Microbiology Laboratory Biology, Faculty of Mathematics and Science, University of Lampung.  The parameters of this research are the content of chlorophyll and the number of leaves.  This research used a completely Randomized Design with 7 treatments that are A1 (24 hours of mixed bromelain), A2 (48 hours of mixed bromelain), A3 (72 hours of mixed bromelain), A4 (24 hours of pure bromelain), A5 (48 hours of pure bromelain), A6 (72 hours of pure bromelain), and A7 (control).  Based on this research the value of the highest chlorophyll A on A6 treatment is 0,655.  A value of the highest on chlorophyll B on A6 treatment that 0,429.  The highest total chlorophyll on the A6 treatment is 1,048.  Based on this research the A4 treatment has the highest average number of leaves compared to other treatments.    
APPLICATION OF BROMELAIN LITTER SOLID COMPOST INDUCED BY LIGNINOLITIK Trichoderma sp. FUNGUS TOWARDS NUMBER OF LEAVES AND CHLOROPHYL CONTENT CHILI PLANTS (Capsicum annuum L.) Jihan Haura; Bambang Irawan; Salman Farisi; Yulianty Yulianty
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.166

Abstract

Piles of uncontrolled pineapple peel waste have a negative impact that will affect various aspects of life, either directly or indirectly. Therefore, pineapple peel waste that can no longer be processed can be used for making compost. The taste of pineapple can be degraded biologically with the help of enzymes that can be produced by microorganisms. One of the microorganisms that can support the decomposition process is a group of fungi. In this study, the fungus Trichoderma sp. (Bioggp 5) is a ligninolytic fungus. Trichoderma sp. is a saprophytic soil microorganism that attacks natural pathogenic fungi and is beneficial to plants. The purpose of this study was to determine the effect of solid bromelain compost inoculum of ligninolytic fungi (Trichoderma sp.) on leaf number and chlorophyll content of chili plants (Capsicum annum L.) and the best dose of compost that could be used. This study used a completely randomized design (CRD) with 7 treatments, namely T0 (control), T1 (1.4% pure bromelain), T2 (1.7% pure bromelain), T3 (2% pure bromelain), T4 (1, 4% bromelain + leaf litter), T5 (1.7% bromelain + leaf litter), T6 (2% bromelain + leaf litter). The parameters used were the number of leaves and chlorophyll content. And the results obtained from this study were the T3 dose (2% pure bromelain) was the best dose for the number of leaves, while the T2 dose (1.7% pure bromelain) was the best dose for chlorophyll content.
RESISTANCE OF RED CURLY CHILI (Capsicum annuum L.) SPROUTS TO FUSARIUM OXYSPORUM INFECTION FROM SEEDS INDUCED BY 0.2 mT Essy Dumayanti; Rochmah Agustrina; Wawan Abdullah Setiawan; Eti Ernawiati; Yulianty Yulianty; Lili Chrisnawati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.167

Abstract

Curly red chili (Capsicum annuum L.) is widely used as industrial raw material because it has a spicy taste. The increasing demand for chili is not balanced with the level of production. Chilies are susceptible to disease, one of which is fusarium wilt. The magnetic field affected the physical and chemical properties of water so that it increased peroxidase enzyme activity and plant metabolism. This research was aim to know the protective effect seeds induced by 0.2 mT Magnetic Fiel had given to red curly chili (Capsicum annuum L.) sprouts to Fusarium oxysporum infection. This study was arranged in a completely randomized design (CRD) with 5 treatments consisting of M0F0 (control), M7F0, M7F60, M15F0, M15F60. M0 is a seed not induced by a magnetic field; M7 is seed induced by magnetic field 7 minutes 48 seconds, M15 is seed induced by magnetic field 15 minutes 36 seconds, F0 is sprouted not infected with F. oxysporum and F60 is sprouts infected with F. oxysporum for 60 minutes. Each unit is repeated 5 times. The results of the ANOVA showed that exposure to a magnetic field had a significant effect on increasing plant height at 21, 28, and 35 days after planting (HST); the wet and dry weight of 7 days old plants; and the content of chlorophyll a, b, and total before flowering 21 days after planting. Overall, the 0.2 mT magnetic field treatment for 7 minutes 48 seconds tended to give better results to increase the growth of chili plants against F. oxysporum infection.
ANATOMIC CHARACTERISTICS OF THE STEM OF MANGO (Mangifera spp.) IN BANDAR LAMPUNG M. Ramdan Syahputra; Yulianty Yulianty; Martha Lulus Lande; Suratman Suratman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.183

Abstract

Mango is one of the most widely grown plants in Indonesia. The characteristic that distinguishes one type of mango from another is to observe its anatomy. Anatomical research on the mango branch has not been done much, especially in Bandar Lampung. The purpose of this study was to determine the diameter and pith radius of the mango (Mangifera spp.) branch in Bandar Lampung City. This research was carried out from January to April 2021 at the Botanical Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung. This study uses a survey method with Simple Random Sampling in 20 sub-districts in Bandar Lampung City. Parameters measured included tracheal diameter, tracheal density, arrangement and type of pith radius, pith radius height, and pith radius width. Data analysis used descriptive analysis by explaining the data obtained in the form of tables and photos. The results showed that there were differences in the average size of the tracheal diameter, tracheal density, height, and width of pith radius of the five mango branch samples. The average diameter of the largest trachea is found in Golek and the smallest tracheal diameter is found in Kweni. The highest average tracheal density is found in Arumanis and the smallest tracheal density is found in Golek. The arrangement and type of pith radius of the fifth mango are uniseriate and heterocellular. The average height of the largest pith radius is owned by Indramayu and the smallest is owned by Golek. While the average width of the largest pith radius is owned by Golek and the smallest is owned by Gedong.   
VIABILITY AND PRODUCTION CALCIFYING BACTERIAL ENDOSPORE ON SAND-CEMENT CARRIER Apriliani Devinta; Enny Zulaika
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.184

Abstract

Carbonatogenic bacteria have the ability to precipitate calcium carbonate (CaCO3) and many calcareous areas are found. Some of its species formed endosporas which resistant to harsh physical condition such as very alkaline pH. The objectives of this study were to obtain spore biomass and determine endosporas viability in tested carrier media such as sand-cement. The tested isolates were Bacillus JA1, JB3, SU1, AK4, Lysinibacillus JB2, and Sporosracina JA4. The production of endosporas was carried out on yeast urea broth with a temperature treatment of 70°C for 20 minutes. The formed endosporas were stored in carrier medium of sand, cement, and a mixture of cement sand. Spore viability was conducted using total plate count method. The results showed that all isolates were able to produce endosporas with the highest endosporas dry biomass is Bacillus AK4 which was 196 mg/L. After 2 hours of storage, endosporas were still able to grow by forming colonies on nutrient agar media.
KETERKAITAN JUMLAH DAERAH TERMUTASI PADA GEN β-GLOBIN DENGAN INDEKS KORPUSKULAR PEMBAWA SIFAT β-THALASSEMIA Priyambodo, Priyambodo
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 3 No. 2 (2016)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v3i2.78

Abstract

Thalassemia is a genetic disorder caused by point mutations on the globin gene that decrease the corpuscular index in thalassemia carriers. Three to five percent of Indonesians are thalassemia carriers, with β-thalassemia being the most common type. This research aimed to identify the relationship between the number of mutated regions on the β-globin gene and the decrease in corpuscular index among β-thalassemia carriers. Data was collected from 2012 to 2013 in Yogyakarta. Hematological analysis was performed by assessing the corpuscular index, including mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) at Prodia Laboratory. Molecular analysis was conducted using the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method at the Laboratory of Genetics and Laboratory of Falitma, Biology Faculty, University of Gadjah Mada. Out of a total of 96 individuals screened, there were 9 suspected β-thalassemia carriers with 1 mutated region of the β-globin gene, showing an average MCV of 63.1 fl, MCH of 19.76 pg, and MCHC of 32.34 g/dl. Seven suspected β-thalassemia carriers with 2 mutated regions showed an average MCV of 61.16 fl, MCH of 19.74 pg, and MCHC of 32.3 g/dl. One suspected β-thalassemia carrier with 3 mutated regions showed an average MCV of 64.2 fl, MCH of 19.5 pg, and MCHC of 30.4 g/dl. The number of mutated regions in the β-globin gene was not the main factor affecting the decrease in corpuscular index among β-thalassemia carriers.
THE AIR QUALITY AND NOISE STUDY IN SETTLEMENTS AND METAL SCRAP MELTING FACTORY Wahid Giantara; Tugiyono Tugiyono; Agus Setiawan; Gregorius Nugroho Susanto
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.159

Abstract

Air is an important component for the survival of living things, especially humans. However, air quality has decreased due to pollution. The harmful elements that enter the atmosphere are Carbon Monoxide (CO), Nitrogen Dioxide (NO2), Sulfur Dioxide (SO2), Particulates (PM10), and Ozone (O3). Noise is defined as unwanted sound or sound so that it disturbs and endangers health. Sources of noise are divided into moving and stationary noise sources. The method used in this research is grab sampling by installing an air sampler impinger to capture particles or pollutants as well as taking noise data using a simple method with a Sound Level Meter with a reading every 5 seconds within 10 minutes for one measurement. The results showed that the air quality in residential and workspace areas was still within quality standards. The noise level in residential areas is still acceptable, but the noise level in the production room surpasses the acceptable limit.
PRODUCTION OF AMYLOCELLULOLYTIC ENZYMES AND THEIR VIABILITY ON CARRIER MEDIA BY Bacillus sp. U4 AND Pseudomonas sp. U3 Enny Zulaika; Salma Shavira Rahma Khofifah
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.186

Abstract

Amylase and cellulase are enzymes that are amyocellulolytic. Both are extracellular enzymes that can degrade organic materials, namely starch and cellulose. The purpose of this study was to determine whether Bacillus sp. U4 and Pseudomonas sp. U3 could produce amylocellulolytic enzymes and determine their viability after being incubated for 2 hours on the husk, peat, and sawdust carrier media. Screening for the presence of amylase was carried out on a selective medium, namely nutrient agar-amylum 0.5 %, and cellulase on carboxymethyl cellulose-agar medium. Both isolates were inoculated into sawdust, husk, peat carrier media and incubated for 2 hours. Viability was observed using the total plate count method. Bacillus sp. U4 and Pseudomonas sp. U3 can produce amylase and cellulase. The best isolate viability after incubation for 2 hours was found in husk carrier media with 2.13 x 105 CFU/gr of husk media.
THE EFFECTIVENESS OF PEPPER LEAVES (Piper nigrum L.) AS A LARVACIDE OF Aedes aegypti MOSQUITO Yuyun Solihat; Emantis Rosa; Gina Dania Pratami; Nuning Nurcahyani
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.187

Abstract

Dengue Hemorrhagic Fever (DHF) cases are quite high in Indonesia. One of the causes of high dengue cases is the high vector population, namely the Aedes aegypti mosquito. Mosquito control Ae. aegypti one of them uses larvicides to break the chain of reproduction. The use of chemical-based larvicides can be bad because it can pollute the environment and cause resistance. As an alternative, you can use natural larvicides made from plants. One of the plants that have the potential as larvicides are pepper (Piper nigrum L.) because it contains compounds that can be toxic to insects such as saponins, flavonoids, alkaloids, and essential oils. In addition, pepper plants are widely cultivated in Lampung and are easy to obtain. The purpose of this study was to determine the effectiveness of pepper leaf (P. nigrum L.) as larvicide for Ae. aegypti. This study used a completely randomized design (CRD). There was 1 control and 5 treatments in the form of pepper leaf extract with concentrations of 0.2%, 0.4%, 0.6%, 0.8%, and 1%. The test material used was Ae. aegypti instar III. Each treatment used 25 larvae with 4 repetitions. Observations were made after 24 hours of treatment. The data obtained in the form of the number of larval deaths were analyzed using one-way ANOVA and Post hoc LSD test. The results of this study showed a significant difference (p = 0.000), and it was found that a concentration of 1% was the most effective concentration as a larvicide for Ae. aegypti with a larval mortality percentage of 92%.

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