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Contact Name
Achmad Arifiyanto
Contact Email
achmad.arifiyanto@fmipa.unila.ac.id
Phone
+6281252496386
Journal Mail Official
achmad.arifiyanto@fmipa.unila.ac.id
Editorial Address
Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung Jl. Sumantri Brojonegoro No. 1 Bandar Lampung Lampung 35145
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Kota bandar lampung,
Lampung
INDONESIA
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)
Published by Universitas Lampung
ISSN : 23384344     EISSN : 2686200X     DOI : https://doi.org/10.23960/jbekh.vxxx.xxx
This journal focuses on the fields of Agricultural, Animal Sciences, Bioconservation, Biopharmacology, Biotechnology, Biomedical, Biological control, Behavioural ecology, Plant Sciences, Ecology, Fishery, Marine Biology, Fresh Water Biology, Microbiology, Immunobiology, Ecotoxicology, and Parasitology. This journal utilizes both the LOCKSS and CLOCKSS systems to create a distributed archiving system among participating libraries and permits those libraries to create permanent archives of the journal for purposes of preservation and restoration
Articles 223 Documents
Effect of Pyriproxyfen, a Malaria Vector Larvicide, on the Growth and Mortality of Vannamei Shrimp Putri, Sriana; Setyaningrum, Endah; Farisi, Salman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 1 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i1.258

Abstract

Anopheles mosquito shared a breeding site with the vannamei shrimp (Litopenaeus vannamei). Anopheles mosquito larvae commonly live in both stagnant fresh and brackish water. The potential breeding habitat of Anopheles mosquito larvae may be rice fields, pools or ponds, swamps, ditches, and slow-current streams. One of the effective strategies to control malaria is to apply chemical insecticides. Pyriproxifen is a larvicide that contains juvenile hormone-like compounds. Juvenile hormone-like compounds can affect mosquito morphogenesis, characterized by the failure of the larvae to develop into pupae. With a completely randomized design, the shrimp fry was divided into 4 groups,  6 repetitions, 20 individuals each. Shrimp fry in each experimental unit was placed and maintained in plastic jars containing 2.5 L of brackish water. The results showed that the insect growth regulator (IGR) with the active ingredient pyriproxyfen at various concentrations had no significant effect on the growth, mortality, and survival of vannamei shrimp fry.  It can be concluded therefore that pyriproxifen larvicide was safe to be used for controlling malaria mosquito vectors in hatcheries and breeding ponds of vannamei shrimp.
Diversity and Community Structure of Butterly in Teijsmann and Soedjana Kassan Park, Bogor Botanical Garden Rizkawati, Vina; Asmara, Yustika Tri; Khairiyyah, Anisah; Perdani, Nindyra Karimah; Fitriasari, Sheryl; Savira, Ananda Nuri; Pasaribu, Pinta Omas; Asharo, Rizal Koen; Priambodo, Rizky
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 1 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i1.270

Abstract

As a megadiversity country, Indonesia is home to a large number of flora and fauna, one of which is the butterfly. The Bogor Botanical Garden, situated at the center of Bogor City, not only serves as a green open space but also as a conservation area for plants and animals composed in it.  This study aimed to assess the diversity of butterfly species in Teijsmann Park and Soedjana Kassan Park, Bogor Botanical Garden through inventory and identification.  Sampling was carried out at spots that were 100 meters apart from each other on a walked-line transect defined at the two parks. The diversity parameters assessed and analyzed are relative abundance, relative frequency, Shannon-Wiener (H’) diversity index, Evenness index (E), and dominance.  In a total of 202 sample individuals, there were identified 38 species of butterflies belonging to 5 families namely Papilionidae, Pieridae, Nymphalidae, Lycaenidae, and Hesperiidae. The highest abundance found in Teijsmann Park is Ypthima philomela from the Nymphalidae family and Zizina otis from the Lycaenidae family. Species with the highest frequency are Junonia hedonia and Ypthima philomela from the Nymphalidae family. On the other hand, the highest frequency and abundance found in Soedjana Kassan Park was Leptosia nina from the Pieridae family. Teijsmann Park showed a slightly higher value of diversity and evenness indexes (H'=2.62, E=0.84) when compared to Soedjana Kassan Park (H'=2.50, E=0.76).  
Local Wisdom Of The People Of Kemlaten Village Parengan Sub-District In Utilizing Wild Plants Aprilia, Diah Ayu; Cintamulya, Imas
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 1 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i1.281

Abstract

The community of Kemlaten village has a habit of using wild plants as food and medicine. The purpose of this study was to describe the types of wild plants used by the Kemlaten village community, Parengan District, as food and medicine and as well as their morphology. This quantitative descriptive study was conducted in March-April 2022 in the Kemlaten village area. The results of the study showed that there were 25 species of wild plants used as food and medicine by the Kemlaten villagers. Of the 25 wild plant species, 32% are used as food and 68% as medicine. The results of the morphological analysis of wild plants used as food and medicine by the villagers of Kemlaten are dominated by single-leaf plants and woody stems. The morphology of these wild plants differs from one another according to the type or family of these wild plants.
Characterization of Phosphate Solubilizing Bacteria from Three Types of Soil Rhizosphere and Their Potency to Increase Growth of Corn Plants (Zea mays) Rosita, Risa; Apriana, Edwin; Hazra, Fahrizal; Eris, Deden Dewantara
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 1 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i1.288

Abstract

Pemberian dosis pupuk P (fosfat) yang tinggi tidak sejalan dengan ketersediaan P dalam tanah karena sebagian besar P terikat oleh Al, Fe dan Ca sehingga tidak tersedia bagi tanaman. Pemanfaatan mikrob pelarut P merupakan salah satu upaya dalam meningkatkan ketersediaan P yang dapat diserap oleh tanaman. Tujuan dari penelitian ini adalah untuk mengisolasi, mengkarakterisasi dan menguji potensi mikroba pelarut P yang diisolasi dari tiga jenis rizosfer. Penelitian menggunakan Rancangan Acak Lengkap (RAL) satu faktorial. Serangkaian karakterisasi melalui uji hipersensitivitas, uji pewarnaan gram, uji katalase, uji oksidase, uji kebutuhan oksigen, uji motilitas, kemampuan menggunakan berbagai sumber karbon (glukosa, laktosa, dan sukrosa), uji metil merah, dan uji pertumbuhan pada berbagai pH . Hasil penelitian menunjukkan sebanyak 5 bakteri pelarut P terpilih (Ca-Al-7, Ca-Al-8, Ca-Al-4, Ca-NF-1, dan Ca-NF-3) bukan patogen tanaman, dengan nilai kelarutan fosfat berturut-turut 1,7; 2,9; 2,5; 3,1; 3,2 berpengaruh nyata terhadap parameter tinggi tanaman, jumlah daun, biomassa segar dan kering tanaman Zea mays setelah inokulasi 4 minggu (p < 0,05).
Effect Concentration of Teak Leaf Extract on the Diameter of the Inhibitory Zone Xanthomonas oryzae Prasetyo, Hidayat Nur; Pantiwati, Yuni; Chamisijatin, Lise
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 1 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i1.294

Abstract

Indonesia is an agricultural country with the majority of the population working in the agricultural sector. According to the Central Statistics Agency of Indonesia, rice production in 2022 is estimated at 9.58 million tons, down by 0.04 million tons or 0.41 percent compared to 2021 which was around 9.62 million tons. The rise and fall of rice production was influenced by several problems. One of the causes of the delay in rice production is leaf blight caused by Xanthomonas oryzae pv. oryzae reducing rice yields by 30-40%. This research was to examine the use of teak leaf extract in inhibiting the growth of X. oryzae bacteria. The results showed that there was an effect of various concentrations of teak leaf extract (Tectona grandis) on the inhibition zone of X. oryzae. The effective concentration of teak leaf extract in inhibiting Xanthomonas oryzae bacteria is 20%.
Population Dynamics of Dragonflies at The Paddy Field Ecosystem, Sidorejo, Central Bengkulu Indriani, Gisela Ratna; Singkam, Abdul Rahman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 1 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i1.297

Abstract

The rice fields of Sidorejo Village have the potential for dragonfly diversity. This study aims to analyze fluctuations in the diversity and abundance of dragonflies in three ecosystem conditions of rice fields in Sidorejo Village, Bengkulu Tengah. Sampling using purposive sampling with the cruising method. Data analysis was carried out descriptively through the identification of characteristics to obtain the name of the species. After the species name was obtained, the relative abundance, Shannon Wienner diversity index, and species evenness index were calculated. The results of the study found that the harvest ecosystem had the highest diversity and abundance of dragonflies (13 species and 45 individuals). The species that were consistently found in three types of ecosystems were Orthetrum sabina, O. testaceum, and Pantala flavescens. The species with the highest relative abundance was O. sabina with 32%. The diversity index ranged from 1.32 (post-harvest) to 2.25 (harvest). The highest evenness index was in the post-harvest ecosystem at 0.95, and the lowest was in the harvest ecosystem at 0.87. The results of this study are expected to show that the diversity and abundance of dragonflies fluctuate depending on seasonal ecosystem conditions in the rice fields.
Species Diversity and Cave-Bat Conservation Efforts in Aoma Village, Wolasi District -Southeast Sulawesi Amirullah, Amirullah; Bakkareng, Sitti Wirdhana Ahmad; Risnaldi, La Ode Fabir
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 1 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i1.305

Abstract

Indonesia a tropical country that remains warm all year, has the largest biodiversity. This study aims to determine the diversity of bats of the order Microchiroptera in Rau Cave, Wolasi, Conservation (Protected) Forest area, around Aoma Village, South Konawe Regency. Data collection was carried out using a mist net measuring 6 x 2 meters which were stretched in front of the mouth of the cave at 16.00 before the bats came out looking for food and harvested at 06.00-0.700. The trapped bats were identified using the Sulawesi Bat and its role in the health sector, and other relevant references. The results showed that there were 26 trapped bats consisting of  5 species, namely Rhinolopus arcuatus (Prok Bruk Sulawesi), Hipposideros ater (Barong gauld), Dobsonia viridis (Kubu Hijau), Miniopterus australis (Tomosu australi) and Hipposideros dinops (Barong horsfield). The species found consisted of 2 families, namely the families Rhinolopidae and Hipposideridae, and consisted of 2 genera, namely the genera of Dobsonia and Miniopterus. The diversity index shows a value (H') = 0.986, which indicates a low category in diversity and a Margalef wealth index (R) of 0.26. It is necessary to conserve plant species both for eating insects and for food sources for Microchiroptera bats in their feeding ground
Diversity of Rodentia and Scandentia Species in the Batutegi Protected Forest, Tanggamus Lampung Sitopu, Romekardo; Nurcahyani, Nuning; Dania Pratami, Gina; Kanedi, M
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 2 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i2.199

Abstract

Mamalia kecil seperti Rodentia dan Scandentia adalah salah satu satwa liar yang sering dianggap sebagai hama perusak, sehingga kurang dijaga kelestariannya. Keberadaan Rodentia dan Scandentia dalam ekosistem sangat penting antara lain sebagai sarana penyebaran biji tumbuh-tumbuhan, dan sebagai kontrol terhadap serangga. Penelitian ini dilaksanakan di kawasan Hutan Lindung Batutegi Tanggamus Lampung mulai bulan Mei sampai Juni 2021. Penelitian ini bertujuan untuk mengetahui keanekaragaman, perilaku, dan, faktor lingkungan yang mempengaruhi keberadaan jenis Rodentia dan Scandentia di kawasan Hutan Lindung Batutegi Tanggamus Lampung. Metode yang digunakan dalam penelitian ini adalah metode survei dengan teknik purposive sampling yaitu dengan mencari lokasi ditemukannya Rodentia dan Scandentia, data yang diperoleh ditampilkan dalam bentuk tabel dan gambar serta dihitung indeks keanekaragamannya dengan menggunakan indeks keanekaragaman Shannon-Wiener dan indeks kemerataan. Berdasarkan hasil penelitian, nilai indeks keanekaragaman jenis di blok inti adalah H’= 2,772 termasuk dalam kategori sedang yang terdiri dari sebanyak 26 jenis yang ditemukan. Nilai indeks keanekaragaman jenis di blok pemanfaatan adalah H’= 1,596 yang terdiri dari 15 jenis yang ditemukan. Nilai indeks tersebut tergolong dalam kategori sedang, dimana nilai indeks tersebut tidak lebih dari H’= 3. Nilai indeks kemerataan pada blok inti adalah E= 0,851 tergolong dalam kategori tinggi dengan komunitas stabil, sedangkan pada blok pemanfaatan adalah E= 0,59 tergolong dalam kategori sedang dengan komunitas labil. Hal tersebut dipengaruhi oleh sumber pakan, habitat, dan faktor adanya aktivitas manusia. Aktivitas perilaku satwa yang ditemukan lebih banyak terdapat pada waktu pagi hari dari suku Sciuridae, Tupaiidae pada waktu sore hari, dan suku Muridae dan Hystricidae pada waktu malam hari (nokturnal).   Kata kunci: hutan lindung Batutegi, indeks keanekaragaman, indeks kemerataan, purposive sampling, Rodentia, Scandentia, Small mammals such as Rodentia and Scandentia are one of the wild animals that are often considered as destructive pests, so that their sustainability is not maintained. The existence of Rodentia and Scandentia in the ecosystem is very important, among others, as a means of dispersal of plant seeds, and as a control against insects. This research was conducted in the Batutegi Tanggamus Protected Forest area, Lampung from May to June 2021. This study aims to determine the diversity, behavior, andenvironmental factors that influence the presence of Rodentia and Scandentia species in the Batutegi Tanggamus Protected Forest area, Lampung. The method used in this study is a survey method with purposive sampling technique, namely by finding the location where Rodentia and Scandentia were found, the data obtained are displayed in tables and figures and the diversity index is calculated using the Shannon-Wiener diversity index and evenness index. Based on the results of the study, the value of the species diversity index in the core block was H'= 2.772 which was included in the medium category which consisted of as many as 26 species were found. The value of the species diversity index in the utilization block is H'= 1,596 which consists of 15 species found. The index value belongs to the medium category, where the index value is not more than H'= 3. The evenness index value in the core block is E= 0.851 belonging to the high category with a stable community. Meanwhile, in the utilization block, E= 0.59 belongs to the medium category with unstable community. This is influenced by food sources, habitats, and human activities. Animal behavior activities were found to be mostly found in the morning from the Sciuridae tribe, Tupaiidae in the afternoon, and the Muridae and Hystricidae tribes at night (nocturnal).   Keywords: Batutegi protected forest, diversity index, evenness index, purposive sampling, Rodentia, Scandentia.
Salt Stress Resistance of In Vitro Selection Results-Moon Orchid (Phalaenopsis amabilis (L.) Blume) Nurcahyani, Endang; Septiani, Dwi; Yulianty, Yulianty; Mahfut, Mahfut
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 2 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i2.215

Abstract

Anggrek bulan adalah satu jenis anggrek yang banyak diminati karena mempunyai keindahan yang dapat dilihat dari ukuran, bentuk, dan warna–warni bunganya sehingga menjadikan produksi anggrek bulan meningkat, tetapi terdapat kendala lahan pertanian yang semakin luas dialihfungsikan ke sektor lainnya dan lahan kurang produktif akibat cekaman garam atau salinitas. Salah satu cara alternatif yang efisien dan efektif untuk mengatasi cekaman garam yaitu dengan menggunakan varietas yang toleran terhadap cekaman garam dengan agen seleksi yaitu NaCl. Penelitian ini bertujuan untuk menentukan tingkat konsentrasi NaCl yang toleran terhadap anggrek bulan dan menentukan tingkatan resistensi anggrek bulan terhadap cekaman garam (NaCl) secara in vitro. Rancangan percobaan pada penelitian ini menggunakan Rancangan Acak Lengkap dengan 5 taraf konsentrasi NaCl 0 % ; 0,25 % ; 0,50 % ; 0,75 %, dan 1 % pada medium Vacin and Went. Parameter yang diamati yaitu, persentase jumlah planlet, visualisasi planlet, tinggi, jumlah daun, indeks stomata, berat basah, kandungan klorofil, dan indeks resistensi cekaman garam. Data kuantitatif dari setiap parameter dianalisis dengan menggunakan Analisis Ragam pada taraf nyata 5% dan uji lanjut dengan Uji Tukey pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa tingkat konsentrasi NaCl 0,25% sampai 0,50% yang ditoleransi oleh tanaman anggrek bulan secara in vitro terdapat pada kisaran 0,25-0,5 dikategorikan cekaman sedang, sedangkan konsentrasi NaCl 0,75% sampai 1% terdapat pada kisaran 0,5-1,0 yang dikategorikan cekaman berat dan tingkatan resistensi anggrek bulan seluruh konsentrasi adalah resistensi moderat.   Kata kunci: cekaman garam, NaCl, Phalaenopsis amabilis (L.) Blume, resistensi, seleksi in vitro, Moon orchid is the one of orchids that most people like because it has a beauty that can be seen on its size, shape, and its colors so that it makes the production of moon orchid becoming increase, but has a problem such as the larger of agricultural land that converted into other sector, the land becomes less productive because of the salt stress or salinity. The one alternative way that efficient and effective to overcome the salt stress is with using varieties that are tolerant to salt stress with selection agent such as NaCl. This research aims to determine the level of of NaCl concentrations that are tolerant to moon orchid and determine the level of resistance of moon orchid with the salt stress by in vitro. The experimental design of this research used completely randomized design with 5 concentration level NaCl 0%; 0,25%; 0,50%; 0,75%; and 1% in Vacin and Went medium. Parameters observed were plantlet percentage, plantlet visualization, height, number of leaves, stomata index, wet weight, chlorophyll content, and salt stress resistance index. The quantitative data from each parameter is analyzed by using Analysis of Variance at 5% significance level and further test with Tukey test at the significant level 5%. The result of this research showed that the concentration level of NaCl 0,25% to 0,50% that tolerated by moon orchid plants by in vitro was in the range of 0,25 - 0,5 categorized by moderate stress, and 0,75% to 1% was in the range 0,5 – 1,0 that categorized with severe stress and the resistance level of the whole concentration in moon orchid is moderate resistance.   Keywords: NaCl, Phalaenopsis amabilis (L.) Blume, selection in vitro, salt stress, resistance  
Quantitative and Descriptive Paradermal Anatomy of Dendrobium discolour and Phalaenopsis amabilis Orchid Leaves Handayani, Tundjung Tripeni; Pramono, Eko
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 2 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i2.216

Abstract

Dendrobium dan Phalaenopsis merupakan sumber daya genetik anggrek  yang semakin langka, sehingga perlu dilakukan upaya pemuliaan untuk melestarikannya. Tujuan penelitian ini untuk mengetahui karakter morfologi dan anatomi Dendrobium dan Phalaenopsis. Metode yang digunakan yakni sayatan paradermal pada epidermis daun anggrek yang diamati secara mikroskopik, kuantitas anatominya, dan kualitas gambarnya. Penelitian ini menggunakan faktor tunggal dengan dua jenis anggrek yang digunakan dalam rancangan acak kelompok, serta variabel kualitatif dan kuantitatif. Hasil penelitian menunjukkan bahwa kedua permukaan Dendrobium memiliki Panjang dan lebar stomata yang hampir sama, sedangkan pada Phalaenopsis pada permukaan bawah daun memiliki ukuran stomata yang sedikit lebih besar dibandingkan dengan permukaan atas. Dendrobium memiliki bentuk sel epidermis atas heksagonal, jenis stomata parasite dan bagian bawah tetrasitik, sedangkan daun Phalaenopsis di permukaan bawah dan atas adalah tetrasitik. Stomata terdapat pada permukaan atas dan bawah daun anggrek Dendrobium dan Phalaenopsis. Berdasarkan dari penelitian yang telah dilakukan terdapat perbedaan pada jumlah stomata, bentuk sel epidermis dan jenis stomata pada permukaan daun atas dan bawah anggrek Dendrobium maupun Phalaenopsis.   Kata kunci: Anatomi, Anggrek, Dendrobium, Paradermal, Phalaenopsis.   Dendrobium and Phalaenopsis are genetic resources of orchids that are increasingly rare, so breeding efforts are needed to preserve them. The purpose of this study was to determine the morphological and anatomical characters of Dendrobium and Phalaenopsis. This research was conducted in April-September 2021 at the Botanical Laboratory of the Biology Department, University of Lampung. The method used is a paradermal incision on the epidermis of orchid leaves which was observed microscopically, the anatomical quantity, and the image quality. This study used a single factor with two types of orchids used in a randomized block design, as well as qualitative and quantitative variables. The results showed that the two surfaces of Dendrobium had almost the same length and width of stomata, while Phalaenopsis on the lower surface of the leaves had stomata size which was slightly larger than the upper surface. Dendrobium has a hexagonal upper epidermal cell shape, parasite stomata type and the bottom is tetracytic, while Phalaenopsis leaves on the lower and upper surfaces are tetracytic. Stomata are found on the upper and lower surfaces of the leaves of Dendrobium and Phalaenopsis orchids. Based on the research that has been done, there are differences in the number of stomata, the shape of the epidermal cells and the type of stomata on the upper and lower leaf surfaces of Dendrobium and Phalaenopsis orchids.   Keywords: Anatomy, Orchid, Dendrobium, Paradermal, Phalaenopsis.