cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Prof. Dr. Elna Karmawati
Contact Email
littri_puslitbangbun@yahoo.co.id
Phone
+62251-8313083
Journal Mail Official
littri_puslitbangbun@yahoo.co.id
Editorial Address
Jalan Tentara Pelajar No. 1, Cimanggu, Bogor 16111
Location
Kota bogor,
Jawa barat
INDONESIA
Jurnal Penelitian Tanaman Industri (Littri)
Published by Pusat Penelitian dan Pengembangan Perkebunan
ISSN : 08538212     EISSN : 25286870     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal Penelitian Tanaman Industri (JLITTRI) aims to publish primary research articles of current research topics, not simultaneously submitted to nor previously published in other scientific or technical ojournals. General review articles will not be accepted. The journal maintains strict standards of content, presentation,and reviewing. SCOPE The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of agricultural science in Indonesia including: Estate crops; Soil science; Climate science; Agronomy; Plant breeding; Biotechnology; Genetic resources; Plant pathology; Plant physiology; Entomology; Farming system; Postharvest technology; Socio-economic agriculture; Environment; Agricultural extension. The journal publishes Indonesian or English articles. Since the year of 2017, the jurnal is published twice a year in (June and December).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 504 Documents
 17   18   19   20   21 
POTENSI BAKTERI ANTAGONIS DALAM MENEKAN PERKEMBANGAN PENYAKIT LAYU BAKTERI JAHE KARDEN MULYA; SUPRIADI SUPRIADI; ESTHER M. ADHI; SRI RAHAYU; NURI KARYANI
Jurnal Penelitian Tanaman Industri Vol 6, No 2 (2000): September, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v6n2.2000.37-43

Abstract

Potency of antagonist bacteria in inhibiting the bacterial wilt disease progress gingerBacterial wilt disease caused by Pseudomonas solanacearum is an important disease in ginger plant (Zingiber oicinale Rose.) in Indonesia. The objective of this research was to study the effectiveness of biological agents in inhibiting the progess of bacterial wilt on ginger. The research was conducted at the geenhouse of Research Institute for Spice and Medicinal Crops in 1997-1998 then was followed with a ield experiment at Sukamulya Experimental Garden in 1998/1999. At the greenhouse experiment seven kinds of antagonists bacteria were formulated either individually or combination. Pseudomonas fluorescens (PF), P. cepacia (PC) and Bacillus sp. (BC), mixtue of PF + PC, mixtue of PC + BC, mixture of PF + BC, and mixtue of PF + PC + BC were tested in the geenhouse on ginger plant cultivars putih besar grown in the pot containing soil formerly used for growing P. solanacearum infected tomato. I he results of this experiment indicated that the combination of PF+PC+BC significantly suppressed the progress of bacterial wilt disease compaed to control and other tested combination. In the ield experiment carried out at the bacterial wilt disease endemic area the combinations of PF+PC+BC, Trichoderma harzianum (Bit-1) and combination of PF+PC+BC+BII-I were tested with two levels of application, i.e. two applications with two months interval and 4 application with one month interval. The results showed that the application of antagonists bacteria inhibited Ihe bacterial wilt disease progress and signiicantly increased ginger rhizome yield. The yield of the rhizome from the plants teated with different intervals were not different. However, the application of the antagonist bacteria were not able to eradicate ginger infection by Pseudomonas solanacearum thoroughly.
EFEKTIVITAS EKSTRAK METANOL DAUN SALAM (Eugenia polyantha) DAN DAUN JERUK PURUT (Cytrus histrix) SEBAGAI ANTIJAMUR PADA PERTUMBUHAN Fusarium oxysporum RITA NOVERIZA; MIFTAKHUROHMAH MIFTAKHUROHMAH
Jurnal Penelitian Tanaman Industri Vol 16, No 1 (2010): Maret 2010
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v16n1.2010.6-11

Abstract

ABSTRAKMinyak atsiri dan ekstrak dari beberapa tanaman telah banyakditeliti sebagai bahan pestisida nabati dan telah tersedia secara komersial.Beberapa penelitian menunjukkan daun salam memiliki aktivitas antijamurdan antibakteri. Jeruk purut mengandung citronella yang dikenal bersifatantijamur. Penelitian yang bertujuan untuk mengetahui pengaruh ekstrakmetanol daun salam dan daun jeruk purut terhadap pertumbuhan Fusariumoxysporum telah dilakukan di laboratorium penyakit Balittro pada bulanJanuari sampai Februari 2008. Penelitian ini terdiri dari tiga kegiatan : (1)Uji ekstrak metanol daun salam dan jeruk purut terhadap pertumbuhanvegetatif F. oxysporum pada media padat. Parameter yang diamati adalahdiameter pertumbuhan F. oxysporum. (2) Uji ekstrak metanol daun salamdan jeruk purut terhadap pertumbuhan generatif F. oxysporum pada mediacair. Parameter yang diamati adalah jumlah konidia yang diproduksi danberat hifa. (3) Uji ekstrak metanol daun salam dan jeruk purut terhadappenghambatan perkecambahan konidia F. oxysporum. Perkecambahankonidia diamati pada 0, 2, dan 4 jam setelah perlakuan. Rancangan yangdigunakan dalam ketiga kegiatan tersebut adalah rancangan acak lengkap,dengan tiga ulangan. Hasil pengujian menunjukkan bahwa ekstrak metanoldaun salam menghambat pertumbuhan vegetatif F. oxysporum. Persentasepenghambatan tertinggi adalah 57,16% pada konsentrasi 5%. Pada mediacair, ekstrak daun salam efektif menurunkan jumlah konidia dan berat hifa.Ekstrak daun salam juga mampu menghambat perkecambahan spora.Persentase penghambatan perkecambahan konidia pada perlakuan ekstrakdaun salam 3% sebesar 84,67% setelah 4 jam inkubasi. Ekstrak metanoldaun jeruk purut konsentrasi 5% efektif menghambat pertumbuhanvegetatif  F.  oxysporum  pada  media  padat  dengan  persentasepenghambatan 95,60 %. Pada media cair, ekstrak jeruk purut (semuakonsentrasi yang diuji) juga mampu menurunkan jumlah konidia dan berathifa. Selain itu, ekstrak metanol daun jeruk purut juga efektif menghambatperkecambahan konidia F. oxysporum hingga 77,00 % setelah 4 jaminkubasi.Kata kunci : Eugenia polyantha, Cytrus histrix, F. oxysporum, metanol,penghambatanABSTRACTThe Effectiveness of Methanol Extract of Bay Leaf(Eugenia polyantha) and Kaffir Lime Leaf (Cytrushistrix) as Antifungal on Growth of F. oxysporumEssential oils extracted from many plants have been investigated forbotanical pesticide source. There are several commercial pesticidescontaining essential oil. Kaffir lime containing citronella is known asantifungal material. Many research showed that bay leaf has antifungal andantibacterial activity. The objective of this research was to find out theeffect of metanol extracts of bay and kaffir lime leaves on F. oxysporumgrowth. The experiment was conducted from January to February 2008 atPhytopathology Laboratory of Indonesian Medicinal and Aromatic CropsResearch Institute (IMACRI). The experiment was consisted of 3activities : (1) Test of methanol extract of bay leaf and kaffir lime leaf onvegetative growth of F. oxysporum in solid media. Fungal colony diameterwas recorded each day. (2) Test of methanol extract of bay and kaffir limeleaves on generative growth of F. oxysporum in liquid media. Conidiaproduction and hifa weight were observed. (3) Test of methanol extract ofbay and kaffir lime leaves on inhabitation of conidia germination of F.oxysporum. The germination of conidia was observed at 0, 2, 4 hours aftertreatment. Experiment was designed using completely randomized designwith three replications. The result showed that methanol extract of bay leafinhibited the growth of F. oxysporum in solid media. The highest growthinhibition was 57.16% at 5% extract. In liquid media, methanol extract ofbay leaf decreased conidia production and hifa weight significantly.Methanol extract of bay leaf inhibited conidia germination. The percentageof inhibition of conidia germination was 84.67% at 3% extract. Methanolextract of Kaffir lime leaf inhibited the growth of F. oxysporumsignificantly. The percentage of inhibition was 95.6% at 5% extract. Inliquid media, methanol extract of kaffir lime leaf decreased conidiaproduction and hifa weight at all of concentration of extract. Methanolextract of kaffir lime leaf inhibited conidia germination. The percentage ofinhibition of conidia germination was 77.00% at 3% extract.Key words : Eugenia polyantha, Cytrus histrix, inhibition, F. oxysporum,metanol
MULTIPLIKASI TUNAS DAN AKLIMATISASI PEGAGAN (Centella asiatica L.) PERIODE KULTUR LIMA TAHUN NATALINI NOVA KRISTINA; DEDI SURACHMAN
Jurnal Penelitian Tanaman Industri Vol 14, No 1 (2008): Maret 2008
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v14n1.2008.30-35

Abstract

ABSTRAKPegagan (Centella asiatica L.) adalah tanaman obat yangmengandung zat asiaticotik sebagai obat alzaimer dan penghalus kulit.Tanaman ini telah diperbanyak sejak tahun 2000. Penelitian dilakukan diLaboratorium Kultur Jaringan dan Rumah Kaca Kelompok PenelitiPlasma Nutfah dan Pemuliaan, Balai Penelitian Tanaman Obat danAromatik, Bogor dari bulan Januari 2000 sampai dengan Juni 2005.Penelitian ini bertujuan untuk melihat daya multiplikasi tunas setiapperiode subkultur dimulai dari tahun kedua sampai periode lima tahun.Media yang digunakan adalah MS + BA 0,1 mg/l. Pengamatan dilakukanpada jumlah tunas, jumlah daun dan visual tunas pada umur 2, 3, 4 dan 5tahun pada dua periode subkultur setiap tahunnya. Penelitian disusundengan rancangan acak lengkap, masing-masing terdiri atas 10 botol yangmerupakan ulangan dan setiap botol terdiri atas 1 eksplan. Untuk re-mediaterhadap tanaman yang terlihat berubah digunakan media MS + BA (0;0,1; 0,2; 0,3) mg/l. Selanjutnya untuk perakaran dilakukan pada media MS+ IAA (0,1 dan 0,2); MS + NAA (0,1 dan 0,2) mg/l serta MS + IBA (0,1dan 0,2) mg/l. Plantlet utuh yang terbentuk selanjutnya diaklimatisasi padamedia tanah + pupuk kandang dan tanah + sekam dengan perbandingan 1 :1. Hasil penelitian menunjukkan bahwa daya multiplikasi tunas optimumterjadi pada tahun ketiga, dan memasuki tahun keempat dan kelimamenurun yang diiringi dengan perubahan eksplan yang terlihat padatangkai daun yang terbentuk. Akar terpanjang dan terbanyak yangterbentuk didapat pada media IAA 0,2 mg/l dengan penampilan yangkurus dan rapuh. Keberhasilan aklimatisasi sangat rendah, tetapi plantletmampu beregenerasi dengan baik dan terlihat tumbuh normal. Dari hasilperbanyakan terlihat bahwa jumlah anakan, jumlah daun, panjang stolondan jumlah bunga lebih tinggi dibandingkan yang tumbuh pada mediasekam, berturut-turut : 6,77; 7,30; 46,50 cm dan 8,31. Sementara padamedia sekam komponen yang dominan adalah panjang tangkai daun yakni9,75 cm.Kata kunci : Pegagan, Centella asiatica L., multiplikasi, tunas, aklima-tisasi, penyimpanan, Jawa BaratABSRACTShoot multiplication and acclimatization of gotuloca(Centella asiatica L.) five years after conservation by invitro cultureGotuloca (Centella asiatica L.) is a medicinal crop containingasiaticotic as alzaimer and skin revitalizer. This crop has beenmultiplicated in vitro since 2000. This research was carried out in thelaboratory and glasshouse of Breeding and Germplasm Group in theIndonesian Medicinal and Aromatic Crops Research Institute (IMACRI)from January 2000 to June 2005. The objective of the research was to findout the viability of shoots multiplication after two year to five year period,with media MS + BA 0.1 mg/l. The parameters observed were the numberof shoots, the number of leaves at the age 2, 3, 4 and 5 years from twosubculture periods every year. The treatments were arranged in acompletely randomized design, each replication consisted of 10 bottles andeach bottle consisted of 1 explant. After subculture the ex-plant were re-media in medium MS + BA (0; 0,1; 0,2; 0,3) mg/l. The rooting mediabefore glasshouse were : MS + IAA ( 0,1 and 0,2); MS + NAA ( 0,1 and0,2) mg/l; and MS + IBA ( 0,1 and 0,2) mg/l. The plantlets formed wereacclimatized using soil + cattle manure and soil + rice husk withcomparison 1:1. Research result indicated that the optimum viabilitymultiplication was achieved in the third year, and it decreased after thefourth and fifth years with change in explant forming the petiole. Thelongest and plantlet roots were formed through media IAA 0.2 mg/l withbrittle and thin appearance, but the plantlets were able to regenerate betterand grow normal. The acclimatization was not very successful but theplantlets could regenerate and grew normally. The multiplication showedthat the number of stumps, leaves, stolons and flowers were : 6,77; 7,30;46,50 cm and 8,31 respectively. In rice husk media the dominantcomponent was pedicle length 9,75 cm.Key words : Gotuloca, Centella asiatica L., multiplication, shoot,acclimatization, conservation, East Jav
BIOLOGI Aspidomorpha miliaris, F. PADA BEBERAPA KONSENTRASI EKSTRAK DAUN GAMBIR . ADRIA; HERWITA IDRIS
Jurnal Penelitian Tanaman Industri Vol 10, No 2 (2004): Juni 2004
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v10n2.2004.51-58

Abstract

Aspidomorpha miliaris. F. (Coleoptera: Chrysomelidae) termasuk hama penting pada tanaman Ipomoeaceae, Convolvulaceae dan Cucurbitaceae yang pengendalian populasinya perlu diarahkan memakai insektisida botanis, mengingat produk dai tanaman ini umumnya bcroricntasi pangan. Gambir (Uncaria gambir, Roxb), merupakan salah satu tanaman sumber bahan pestisida botanis yang potensial, karena daun tanaman ini mengandung senyawa kimia berupa catechin, tannin catecu (tannat), querchitin dan beberapa senyawa lainnya. Sehubungan dengan itu lelah dilakukan penelitian tentang aspek biologis A. miliaris pada beberapa konsentrasi ekstrak daun gambir di Kebun Percobaan Laing Solok mulai bulan Juli 2001 sampai Januari 2002. Penelitian memakai rancangan acak lengkap dengan 7 perlakuan dan 4 ulangan, masing-masing perlakuan adalah ekstrak daun gambir konsentrasi 1000, 2000, 3000, 4000, 5000, 7500 ppm dan lanpa ekstrak (0 ppm) sebagai kontrol. Parameter pengamatan meliputi persentase kematian larva, pupa dan imago, persentase penetasan telur dan emcrgensi pupa, fckunditi imago, penurunan volume makan dan panjang siklus hidup. Hasil penelitian menunjukkan bahwa ekstrak daun gambir mcmiliki sifat insektisidal yang baik sehingga mampu mempengaruhi aspek biologis dari serangga A. miliaris. Dalam konsentrasi 5000 ppm dan 7500 ppm bahan ini dapat menyebabkan kematian terhadap larva instar III, IV, V, VI sebesar 65.20%, 62.00%, 55.20%, 46.80% dan 79.40%, 74.20%, 67.80%, 57.00%. Kedua konsentrasi juga mampu meningkatkan kematian imago 6.85% dan 7.15% menekan persentase penetasan lelur (18.09% dan 21.94%) dan emergensi pupa (16.72% dan 20.82%). Di samping itu konsentrasi diatas dapal menekan volume makan larva dan imago, mempengaruhi masa prcreproduktif dan tingkat fckunditi imago seta memperpanjang siklus hidup.Kata kunci: Uncaria gambir Roxb., insektisida botanis, ekstrak daun, Aspidomorpha miliaris, F., aspek biologis ABSTRACTBiology of Aspidomorpha miliaris F. at several concen¬ trations ofgambier leavesextractAspidomorpha Miliaris F. (Coleoptera Chrysomelidae) is an impotant pest in Ipomoeaceae, convolvulaceae and cucurbitaceae plants. Their population need to be controlled by using botanical insecticide, consideing thai the product from these plants usually be oiented to food. Gambier (Uncaria gambier.Roxb), is a potential source for botanical pesticide, because the leaves contain chemical compounds in the form of catechin, lannin catecu (tannat), querchitin and some other compounds. In connection with that, the research on biologycal aspects of A. millions at some concentration of gambier leaf extract was done in KP. Laing Solok from July 2001 lo January 2002. The research used a completely randomized design with 7 treatments and 4 replications. The concentration of gambir leaf extracts were 1000, 2000, 3000, 4000. 5000, 7500 ppm. 0 ppm as control The paiiiineleis oliseivcd were laivac motality, pupae and Imago, egg exlotion and pupae emergency, imago fecundity, the decrease of eating volume and life cycles. The research result showed that gambier leaf extract had good inseciicidallity and was able to influence the biology aspect A. miliaris insect. At the concentration of 5000 ppm and 7500 ppm this extract caused mortality to the larva instar III, IV, V, VI as much as 65.20% 62.00%, 55.20% 46.80% and 79.40%, 74.20%, 67.80%, 57.00%. Both the concentraions also were able (o increase (he imago motality 6.85% and 7.15%, pressed down the egg exlosion percentage and pupae emergency 18.09% and 21.94% and 16.72% and 20.82%. Besides these concentrations above could press down larvae and imago eating volume, influences the prareproductive period and the imago fecundity level and life cycles.Key words: Uncaria gambir Roxb., botanical insecticide, leaf extract, Aspidomorpha miliaris F., biological aspect
RESPONSE OF FIVE KENAF ACCESSIONS TO SHOOT REGENERATION RULLY D. PURWATI; SUDJINDRO SUDJINDRO; SUDARMADJI SUDARMADJI
Jurnal Penelitian Tanaman Industri Vol 5, No 1 (1999): Juni, 1999
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n1.1999.31-37

Abstract

This expeiment was aimed to study response of fives kenfa accession to shoot regeneration and to establish an appropriate regeneration protocol for kenaf. Theexpeimentwasperformed at Tissue Culture Laboratory Research Institute for Tobacco and Fibre Crops (RITFC), Malang. from June to Octo¬ ber 1997 Cotyledons with plumules attached were used as explanls and culture medium for callus induction was MS-based medium with BAP (2 mg/1) and NAA (0 5 mg/1). Calli produced in this cultures were transferred into MS-based medium containing BAP (2 mg/1) and GA.i (5 mg/1) for shoot initiation. All shoots obtained were then sub-cultured in MS-based medium without regulators (MSO) for root formation. Rooted shoots (plantlets) were acclimatized in the sterile sand and transferred mto sterile soil in the glass house. Results of this experiment showed that the most responsive accessions were Cuba 108/1. followed by KK 60, He 48. PI 324922. and CHN/ 056 H with the average number of shoots per explant 4.32 * 4.21,4.00 + 4.01.3.05 + 2.98,2.80 + 1.47. aid 2.72 + 2.49. respectively, at 40 days ater transfering on shoot regeneration medium Shoots rooted ater 14 days on MSO medium with frequencies of 81 50-93.30%. Healthy plantlets survived and grew well in soil media in the glass house. Therefore, an appropriate shoot regeneration protocol for kenaf was found.
POTENSI BAKTERI ENDOFIT ASAL TANAMAN LADA SEBAGAI PELARUT FOSFAT DAN PENGIKAT NITROGEN SERTA PENGHASIL IAA / Potency of Endophytic Bacteria from Black Pepper as Phosphate Solubilization, Nitrogen Fixation and IAA Production Gusmaini Gusmaini; Andriana Kartikawati
Jurnal Penelitian Tanaman Industri Vol 25, No 1 (2019): Juni, 2019
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v25n1.2019.11-21

Abstract

The potential of endophytic bacteria to promote plant growth has been already well known. However, research on indigenous endophytic bacteria from wild or cultivated pepper plants are still limited.  The research was conducted in Microbiology Laboratory and Glass House of Research Institute for Spice and Medicinal Crops, Bogor, West Java  from June 2015 to April 2016. The objective of this study was to isolate and characterize the potential of endophytic bacteria from cultivated and wild pepper plants as phosphate (P) solubilizing, N2 fixation, and IAA hormone producers. Endophytic bacteria were isolated from the roots and leaves of both cultivated and wild pepper plants. The surfaces part of plant samples were sterilized using alcohol 70% and sodium hypochlorite 2%. Healthy parts of plants (roots and leaves) are selected as samples. Then, 1 g of plant part was crushed into small particles, extracted, filtered and cultured on Trypticase Soy Agar medium, with 3 replications for each dilution. All bacterial isolates were assessed their capability as P solubilizing, N2 fixation, and IAA hormone producer. The result showed the different capability of each endophytic bacteria derived from cultivated and wild pepper plants in promoting plant growth. The results of isolation of endophytic bacteria were obtained by 23 isolates from P. nigrum and 11 isolates from P. colubrinum. The endophytic bacteria could be grouped as followed: four isolates were IAA producers; 21 isolates possessed two functions (10 isolates as N2 fixation and IAA producers,  while 11 isolates as P solubilizing, and IAA producers); and nine isolates have three functions as P solubilizing, N2 fixation, and IAA producer viz. Ca3(1)2, La0(2)1, La2(2)2, La4(1)3, La4(3)1, La4(3)2, La4(3)3, La4(3)4, and Ld0(2)2.  All 34 endophytic bacterial isolates obtained from this study can potentially be used to improve nutrient use efficiency and accelerate plant growth. Furthermore, it is necessary to further examine the role of endophytic bacteria, which have single or multiple functions, in improving plant growth and fertilizer use efficiency for pepper plants.Keywords : Piper nigrum, Piper colubrinum, endophytic bacteria, P solubilizing, N2 fixation, phytohormone.AbstrakPotensi bakteri endofit untuk meningkatkan pertumbuhan tanaman sudah banyak diketahui. Namun bakteri endofit indigenus tanaman lada, baik lada yang sudah dibudidayakan maupun lada liar belum banyak dilakukan penelitian. Penelitian ini dilaksanakan di Laboratorium Mikrobiologi dan Rumah Kaca Balai Penelitian Tanaman Rempah dan Obat Bogor, Jawa Barat pada bulan Juni 2015–April 2016. Penelitian bertujuan untuk mengisolasi dan mengkarakterisasi potensi bakteri endofit yang berasal dari tanaman lada budidaya dan liar sebagai pelarut P, pengikat N, dan penghasil hormon IAA in vitro. Bakteri endofit diisolasi dari akar dan daun tanaman lada budidaya dan lada liar. Permukaan sampel bagian tanaman disteril dengan menggunakan alkohol 70% dan sodium hypochlorite 2%. Bagian tanaman (akar dan daun) yang sehat dipilih sebagai contoh. Setiap bagian tanaman sebanyak 1 g ditimbang kemudian digerus sampai halus kemudian ekstraknya disaring dan ditumbuhkan pada medium Trypticase Soy Agar dengan 3 ulangan untuk tiap pengenceran. Semua isolat bakteri yang diperoleh diukur kemampuannya sebagai pelarut fosfat (P), pengikat N, dan penghasil IAA invitro. Hasil isolasi dan karakterisasi menunjukkan kemampuan setiap bakteri endofit yang berasal dari lada budidaya dan liar yang berfungsi sebagai pemicu pertumbuhan tanaman berbeda-beda. Dari hasil isolasi diperoleh sebanyak 23 isolat dari Piper nigrum dan 11 isolat dari P. colubrinum. Bakteri endofit tersebut dapat dikelompokkan menjadi penghasil IAA (4 isolat), 21 isolat berfungsi ganda (10 isolat sebagai pengikat N2 dan penghasil IAA serta 11 isolat sebagai pelarut P dan penghasil IAA), dan 9 isolat berfungsi ketiganya (pelarut P, pengikat N2 dan sekaligus penghasil IAA).  Hasil penelitian  mengindikasikan bahwa isolat bakteri endofit tersebut dapat dimanfaatkan untuk memacu pertumbuhan tanaman. Peran bakteri endofit tersebut perlu diuji lebih lanjut aktivitas dan kestabilannya dalam meningkatkan pertumbuhan tanaman.Kata kunci : Piper nigrum, Piper colubrinum, lbakteri endofit, pelarut P, pengikat N, fitohormon.
GENETIC CHARACTERIZATION OF SEVERAL PROMISING ACCESSION OF Jatropha curcas L. BASED ON RAPD MARKER MAFTUCHAH MAFTUCHAH; AGUS ZAINUDIN; RULLY DYAH PURWATI; HADI SUDARMO
Jurnal Penelitian Tanaman Industri Vol 17, No 2 (2011): Juni 2011
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v17n2.2011.83-88

Abstract

ABSTRACTThe objective of this research was to obtain genetic relationshipamong 13 Jatropha curcas L. accession plants based on Random Amplified Polymorphic DNA marker. This experiment used 13 accessionsof J. curcas L. potential to have higher seed productivity, including HS-49,SP-16, SP-38, SP-8, SM-33, SP-34, SM-35, IP-1A, IP-1M, IP-1P, IP-2A,IP-2M, and IP-2P. Polymerase Chain Reaction (PCR) was performedusing 10 selected primers of RAPD markers (OPA 2, OPA 9, OPA 13,OPA 15, OPA 18, OPA 19, OPA 20, OPF 8, OPF 10, and OPF 15). PCRproduct was used to determine genetic distance which implemented Un-weighted Pair-Group Method With Arithmetic (UPGMA) procedure andconstructed phylogeny trees using Numerical Taxonomy and MultivariateSystem (NTSYS) software version 1.8. The confidence level of UPGMAwas then tested by Boostrap using WinBoot program. Ten primers used inthis research were able to be applied in genomic DNA of J. curcas L. plantwhich had resulted about four (OPA 19) to ten band numbers (OPA 9)with the band size around 72-1,078 bp. However, OPA 13 primer was notable to give different band size. Genetic relationship analysis has foundtwo main groups, firstly accession plants consisted of HS-49, SP-16, SP-18, SP-8, SM-33, SM-35, and SP-34 (coefficient 0.8). In this group, SP-38clustered with SP-8, and SM-33 with SM-35 (coefficient 0.91). In thesecond group, the accessions consisted of IP-1A, IP-1M, IP-1P, IP-2A, IP-2M, and IP-2P (coefficient 0.78). In this group, accession of IP-1Aclustered with IP-1M (coefficient 0.85), IP-1P with IP-2M (coefficient0.87), and IP-2A with IP-2P (coefficient 0.90). Then, the first and secondgroups formed genetic relationship with coefficient 0.66.Key words: Genetic characterization, Jatropha curcas L., RAPD,molecular marker, promising accession.ABSTRAKPenelitian ini bertujuan untuk mendapatkan informasi keragamangenetik dan hubungan kekerabatan berbagai aksesi jarak pagar terpilihberdasarkan analisis molekuler Random Amplified Polymorphic DNA(RAPD). Penelitian menggunakan 13 aksesi Jatropha curcas yangmemiliki potensi produksi tinggi (HS-49, SP-16, SP-38, SP-8, SM-33, SP-34, SM-35, IP-1A, IP-1M, IP-1P, IP-2A, IP-2M, dan IP-2P). Isolasi DNAgenom J. curcas dilaksanakan dengan metode Zheng yang dimodifikasi.Polymerase Chain Reaction (PCR) dilakukan menggunakan 10 primersRAPD (primer OPA 2, OPA 9, OPA 13, OPA 15, OPA 18, OPA 19, OPA20, OPF 8, OPF 10, dan OPF 15). Produk PCR yang dihasilkan digunakanuntuk menentukan tingkat kekerabatan menggunakan Un-weighted Pair-Group Method With Arithmetic (UPGMA) dan diagram filogenetik denganprogram Numerical Taxonomy and Multivariate System (NTSYS) versi1.8. Kesepuluh primer yang digunakan mampu mengamplifikasi DNAjarak pagar dengan jumlah produk pita antara 4 (primer OPA 19) hingga10 pita DNA (OPA 9), dengan ukuran pita 72-1.078 bp. Primer OPA 13tidak dapat memberikan perbedaan pita DNA. Hasil analisis kekerabatanmenunjukkan adanya dua kelompok utama. Kelompok pertama terdiri atasaksesi HS-49, SP-16, SP-18, SP-8, SM-33, SM-35, dan SP-34 (koefisien0,80). Dalam kelompok pertama, SP-38 berkelompok dengan SP-8, danSM-33 dengan SM-35 (koefisien 0,91). Kelompok kedua terdiri atas aksesiIP-1A, IP-1M, IP-1P, IP-2A, IP-2M, dan IP-2P (koefisien 0,78). Dalamkelompok kedua, IP-1A berkelompok dengan IP-1M (koefisien 0,85), IP-1P dengan IP-2M (koefisien 0,87), dan IP-2A dengan IP-2P (koefisien0,90). Selanjutnya, kelompok pertama dan kelompok kedua membentukkekerabatan pada koefisien 0,66.Kata kunci: Karakterisasi genetik, Jatropha curcas L., RAPD, markamolekuler, aksesi harapan.
PERANAN FAKTOR LINGKUNGAN TERHADAP POPULASI Helopeltis spp. dan Sanurus indecora PADA JAMBU METE ELNA KARMAWATI
Jurnal Penelitian Tanaman Industri Vol 12, No 4 (2006): DESEMBER 2006
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v12n4.2006.129-134

Abstract

ABSTRAKUntuk mengetahui pengaruh faktor lingkungan terhadap tingkatserangan serangga hama utama pada pertanaman jambu mete telahdilakukan penelitian di Kabupaten Lombok Barat. Pengamatan padatanaman contoh telah dilaksanakan selama musim kemarau dan musimhujan dari bulan Juni 2004 sampai dengan Maret 2005 di dua tempat yangberbeda keadaannya yaitu Desa Tanah Sebang dan Sambik Jengkel.Keduanya terletak di Kecamatan Kayangan, Kabupaten Lombok Barat.Dari masing-masing lokasi diamati 40 tanaman contoh secara acak, 20 daripertanaman monokultur dan 20 dari pola tanam campuran jambu metedengan tanaman lainnya. Variabel yang diamati adalah (a) populasiHelopeltis spp. per tanaman, (b) populasi Sanurus indecora per tanaman,(c) banyaknya pucuk terserang Helopeltis spp., (d) banyaknya pucukterserang S. indecora, (e) banyaknya koloni semut per pohon, (f)persentase telur yang terparasit, (g) suhu, kelembaban dan curah hujanharian, (h) jenis tanaman sela yang menjadi tanaman inang alternatif hama,(i) banyaknya musuh/gulma di sekeliling tanaman, (j) jumlah bungahermaprodit dan buah jadi. Hasil penelitian menunjukkan bahwa intensitasserangan dan populasi Helopeltis spp. dan S. indecora jambu meteberbeda antara musim kemarau dan musim hujan. Pada musim kemarau,hanya sisa-sisa serangan Helopeltis spp. pada pucuk yang kelihatan,populasi tidak ditemukan. Populasi S. indecora selalu ada selama musimkemarau. Pada musim hujan yaitu bulan Januari sampai Maret, pucukmulai muncul, populasi dan serangan Helopeltis spp. mulai kelihatan.Tingkat serangan kedua hama tersebut berbeda antara lokasi Tanah Sebangdan Sambik Jengkel. Di Tanah Sebang, persentase pucuk yang diserang S.indecora (23,1%) lebih tinggi dibandingkan dengan pucuk yang diserangHelopeltis (3,8-7,4%), sedang di Sambik Jengkel persentase pucuk yangdiserang Helopeltis spp (43,8-54,6%) lebih tinggi dibandingkan dengan S.indecora (11,5-22,3%). Faktor utama yang memegang peranan adalahtanaman inang alternatif yang berada pada pola tanam campuran, iklimmikro (suhu, kelembaban dan radiasi matahari) serta interaksi antara S.indecora, Helopeltis spp. dan semut predator.Kata kunci : Jambu mete, Anacardium occidentale, faktor lingkungan,Helopeltis spp., Sanurus indecora, populasi, Nusa TenggaraBaratABSTRACTRole of environment factors on the population ofHelopeltis Spp. and Sanurus indecora on cashewplantationAn experiment to find out the effect of environment factors on theattack of insect pests on cashew plantation was carried out in WestLombok District, West Nusa Tenggara Province. The experiment wasconducted in hot and rainy seasons from June 2004 to March 2005 in twolocations : Tanah Sebang and Sambik Jengkel. These were located in thesame district (Kecamatan Kayangan, West Lombok District). In 40 sampleplants were observed : 20 from monoculture and 20 from polyculture(mixed cropping). The variables observed were (a) Helopeltis populationper plant (b) S. indecora population per plant, (c) number of shootsattacked by Helopeltis, (d) number of shoots attacked by. S. indecora, (e)number of ant colonies per plant, (f) percentage of eggs parasitoid, (g)temperature, relative humidity, daily raindrops, (h) type of intercrops asalternative hosts, (i) litters or weeds surrounding the plantation, (j) numberof hermaphrodite flowers and fruits. The research result showed that thepopulation and damage intensity were different between hot and rainyseasons. In dry season, only the symptom of Helopeltis damage was seen.The population S. indecora always existed during the dry season. In therainy season from January to March, shoots started to appear, also thepopulation of Helopeltis. The injury level was different between those twoinsects. At Tanah Sebang the shoots attacked by S. indecora was 23.1%higher than that of Helopeltis (3.8-7.4%), while in Sambik Jengkel, theshoots attacked by Helopeltis was (43.8-54.6%) higher than that of S.indecora (11.5-22.3%). The main factors played roles in the environmentwere alternate hosts, micro climate and interaction between S. indecora,Helopeltis spp. and predator ants.Key words: Cashew, Anacardium occidentale, environment factorsHelopeltis spp., Sanurus indecora, population, West NusaTenggara
DOSIS SUBLETAL S/NPV DAN PENGARUHNYA TERHADAP TRANSMISI VERTIKAL PADA LARVA Spodoptera litura F. IG. A.A. INDRAYANI; T. HADIASTONO; G. MUDJIONO
Jurnal Penelitian Tanaman Industri Vol 9, No 2 (2003): Juni, 2003
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v9n2.2003.55-62

Abstract

Ketcrganlungan pada insektisida kimia dalam pengendalian serangga hama kapas merupakan salah satu alasan pemanfaatan patogen serangga, khususnya S/NPV untuk mengendalikan larva S. litura. Mcskipun secara tcknis agen pengendalian ini cukup potensial mengendalikan hama sasaran, tetapi pemanfaatannya secara luas masih menghadapi banyak kendala, salah satunya adalah tcrbalasnya produk komersial yang menyebabkan aplikasi kurang optimal. Fcnomena transmisi vetikal S/NPV pada generasi hama bcikutnya cukup berpotensi mengendalikan inangnya secara langsung. Penelitian ini dilakukan di l-aboratorium Hama Balai Penelitian Tanaman Tembakau dan Serat Malang, mulai April hingga Agustus 2002. Tujuannya adalah untuk memperoleh informasi mengenai pengaruh dosis subletal S/NPV pada perkembangan larva S. lilura dan potensinya dalam mentransmisikan infeksi S/NPV pada generasi bcrikutnya (FI). Penelitian disusun secara faktorial dengan rancangan acak kelompok (RAK) yang terdiri atas dua faktor. Faktor petama adalah instar larva S. litura terdiri atas dua taraf, yailu: (1) instar ketiga, dan (2) instar kelima. Faktor kedua adalah dosis subletal S/NPV terdiri atas liga laraf, yaitu 5000. 50 000: dan 500 000 PlB'larva. Sebagai pembanding perlakuan digunakan kontrol (tanpa perlakuan S/NPV), dan setiap perlakuan diulang empat kali. Hasil penelitian menunjukkan bahwa pengaruh dosis subletal S/NPV pada larva S. litura instar ketiga dan kelima, selain menyebabkan motalitas larva, juga mengurangi bobot pupa dan jumlah lelur Larva instar ketiga lebih peka icrhadap infeksi S/NPV yang menyebabkan pupanya mcmiliki bobol (erendah, yaitu 211.5 mg pada laraf dosis 50 000 PIB/larva. Rendahnya bobol pupa berkorelasi positif dengan menurunnya jumlah telur imago, yaitu hanya mencapai 30% (502 butir) dari jumlah telur pada kontrol Peningkatan laraf dosis subletal meningkatkan persentase telur ter¬ kontaminasi polihedra, yaitu tertinggi 24% pada taraf dosis 500 000 PIB/larva, dan juga menurunkan daya tetas telur. Sterilisasi berpotensi menghambat transmisi vetikal S/NPV pada generasi berikutnya (FI), sehingga menurunkan persentase motalitas larva neonate FI. Tanpa sterilisasi pada lelur, motalitas larva neonate IT mencapai t 20%, tetapi dengan sterilisasi menyebabkan motalilas larva rendah yaitu t 5%.Kata kunci : Dosis subletal, entomopatogen S/NPV, transmisi vertikal, S. litura, instar, mortalitas, pupa, neonate, cpizootik. kapas ABSTRACTSublelhal dose of SINPV and its effects on vertical transmission on larvae of Spodoptera lituraOne reason of the use of cnlomopathogen is to reduce chemical inscctisides application on cotton pest insect control S/NPV is one of ihe effective entomopathogen to control S. litura larvae. Technically, this biological agent is effective to control the insect pest, however it is not used widely because of its limited commercial product. Solving this problem is needed such as searching for another information about killing potential of S/NPV to the next generation of the insect pest, especially to anticipate the less frequency of its application. Therefore, infection through vetical transmission is one of ihe solutions. The research were conducted al the Laboratory of F-ntomology of Indonesian Tobacco and Fibre Crops Research Institute (ITOFCRI), Malang, from April to August 2003. The aim of the study was to get information about sublcthal doses of S/NPV and its effect to S litura larvae development and vetical transmission potency to Ihe next larval generation (FI) of Ihe insect pesl. This study was arranged in factorial by using Randomized Block Design with 2 factors: (1) instar of larvae (third and fith instar), (2) sublethal doses of S/NPV (5 000; 50 000, and 500 000 PIB.larvac) and one control (untreated) as a comparison to treatments. The result showed thai the effect of sublelhal doses of S/NPV on (he third and fith instars of S. litura larvae was nol only caused mortality, but also less pupal weight and egg numbers. The third instar of larvae was more susceptible to S/NPV infection which caused lowest weight of pupae ca. 211.5 mg at 50 000 PIB/larva level of dose. There was positive correlation between less pupal weight and decreased of egg numbers which only 30% (502 eggs) of that in control Increasing sublethal dose of S/NPV would increased polyhcdra- contaminatcd eggs by 24% at 500 000 PIB/larvae and decrease the egg hatch. Egg surface sterilization would obstruct vetical transmission of S/NPV to their new generation of larvae (FI), so it decreases the percentage of neonate larval motality rom 20% to 5% when untreated and treated surface egg sterilization, respectively.Key words: Sublethal dose, entomopathogenic S/NPV. vetical transmission, S. lilura, instar, mortality, pupae, neonate, epizootic, cotton
KETAHANAN TEMBAKAl) TEMANGGUNG TERHADAP KUTU TEMBAKAU, Myzuspersicae (SULZER) (HOMOPTERA: APHIDIDAE) SUBIYAKTO SUBIYAKTO; GATOT MUDJIONO; SUWARSO SUWARSO; D. A. SUNARTO
Jurnal Penelitian Tanaman Industri Vol 4, No 4 (1998): November, 1998
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v4n4.1998.109-114

Abstract

Resistance in Temanggung tobacco lines to tobacco aphid (Myzus persicae Sulzer)The evaluation on the esistance of Temanggung obacco lines against tobacco aphid was carried out in Malang, from August 19% to March 1997. The experiments consisted of antixenosis and antibiosis resistance. The teatments wee four tobacco lines fom the original population: (1) S.2258, (2) S.2132, (3) S.l%3, (4) S.1965; four lines rom selected population: (5) S.2258/2/1/1, (6) S.2132/2/2/1/1, (7) S.1%3/3/2/1/2/1, (8) S.1%5/2/1/2/1/1; one local line: (9) Kemloko (Gober genjah); two varieties resistant and susceptible o tobacco aphid: (10) Coker 176, and (11) NC95. The treatments wee arranged in randomized block design, with three replications. The esults showed that the two lines from the selection evaluation: S.2258/2/1/1 and S.1%3/3/2/1/2/1 wee resistant to tobacco aphid. Both lines had high density of leaf trichomes, and can be used as resistant resources in plant breeding activities.

Page 19 of 51 | Total Record : 504

 17   18   19   20   21 

Filter by Year

1998 2021


Reset
Filter By Issues
All Issue Vol 27, No 2 (2021): December 2021 Vol 27, No 1 (2021): June, 2021 Vol 26, No 2 (2020): December, 2020 Vol 26, No 1 (2020): June, 2020 Vol 25, No 2 (2019): Desember, 2019 Vol 25, No 1 (2019): Juni, 2019 Vol 24, No 2 (2018): Desember, 2018 Vol 24, No 1 (2018): Juni, 2018 Vol 23, No 2 (2017): Desember, 2017 Vol 23, No 1 (2017): Juni, 2017 Vol 22, No 4 (2016): Desember, 2016 Vol 22, No 3 (2016): September, 2016 Vol 22, No 2 (2016): Juni, 2016 Vol 22, No 1 (2016): Maret, 2016 Vol 21, No 4 (2015): Desember 2015 Vol 21, No 3 (2015): September 2015 Vol 21, No 2 (2015): Juni 2015 Vol 21, No 1 (2015): Maret 2015 Vol 20, No 4 (2014): Desember 2014 Vol 20, No 3 (2014): September 2014 Vol 20, No 2 (2014): Juni 2014 Vol 20, No 1 (2014): Maret 2014 Vol 19, No 4 (2013): Desember 2013 Vol 19, No 3 (2013): September 2013 Vol 19, No 2 (2013): Juni 2013 Vol 19, No 1 (2013): Maret 2013 Vol 18, No 4 (2012): Desember 2012 Vol 18, No 3 (2012): September 2012 Vol 18, No 2 (2012): Juni 2012 Vol 18, No 1 (2012): Maret 2012 Vol 17, No 4 (2011): Desember 2011 Vol 17, No 3 (2011): September 2011 Vol 17, No 2 (2011): Juni 2011 Vol 17, No 1 (2011): Maret 2011 Vol 16, No 4 (2010): Desember 2010 Vol 16, No 3 (2010): September 2010 Vol 16, No 2 (2010): Juni 2010 Vol 16, No 1 (2010): Maret 2010 Vol 15, No 4 (2009): Desember 2009 Vol 15, No 3 (2009): September 2009 Vol 15, No 2 (2009): Juni 2009 Vol 15, No 1 (2009): Maret 2009 Vol 14, No 4 (2008): Desember 2008 Vol 14, No 3 (2008): September 2008 Vol 14, No 2 (2008): Juni 2008 Vol 14, No 1 (2008): Maret 2008 Vol 13, No 4 (2007): DESEMBER 2007 Vol 13, No 3 (2007): SEPTEMBER 2007 Vol 13, No 2 (2007): JUNI 2007 Vol 13, No 1 (2007): MARET 2007 Vol 12, No 4 (2006): DESEMBER 2006 Vol 12, No 3 (2006): SEPTEMBER 2006 Vol 12, No 2 (2006): JUNI 2006 Vol 12, No 1 (2006): MARET 2006 Vol 11, No 4 (2005): DESEMBER 2005 Vol 11, No 3 (2005): SEPTEMBER 2005 Vol 11, No 2 (2005): JUNI 2005 Vol 11, No 1 (2005): Maret 2005 Vol 10, No 4 (2004): Desember, 2004 Vol 10, No 3 (2004): September, 2004 Vol 10, No 2 (2004): Juni 2004 Vol 10, No 1 (2004): Maret 2004 Vol 9, No 4 (2003): Desember 2003 Vol 9, No 3 (2003): September, 2003 Vol 9, No 2 (2003): Juni, 2003 Vol 9, No 1 (2003): Maret, 2003 Vol 8, No 4 (2002): Desember, 2002 Vol 8, No 3 (2002): September, 2002 Vol 8, No 2 (2002): Juni, 2002 Vol 8, No 1 (2002): Maret, 2002 Vol 7, No 4 (2001): Desember, 2001 Vol 7, No 3 (2001): September, 2001 Vol 7, No 2 (2001): Juni,2001 Vol 7, No 1 (2001): Maret, 2001 Vol 6, No 3 (2000): Desember, 2000 Vol 6, No 2 (2000): September, 2000 Vol 6, No 1 (2000): Juni, 2000 Vol 5, No 4 (2000): Maret, 2000 Vol 5, No 3 (1999): Desember, 1999 Vol 5, No 2 (1999): September, 1999 Vol 5, No 1 (1999): Juni, 1999 Vol 4, No 6 (1999): Maret, 1999 Vol 4, No 5 (1999): Januari, 1999 Vol 4, No 4 (1998): November, 1998 More Issue