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Arif Rahman Hikam
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BioEksakta : Jurnal Ilmiah Biologi Unsoed
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 27148564     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
Jurnal BioEksakta menerbitkan artikel ilmiah hasil penelitian dalam bidang biologi umum termasuk Ekologi dan konservasi, Taksonomi dan Struktur, Biogeografi, Evolusi, Biodeversitas, Fisiologi dan Reproduksi, Biologi sel, Biologi Molekuler dan Genetika.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 401 Documents
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Deteksi Molekuler Virus Dengue dan Chikungunya pada Nyamuk Aedes spp. di Kecamatan Cilongok Fitriana, Dwi Iva; Kusmintarsih, Endang Srimurni; Ambarningrum, Trisnowati Budi
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.1815

Abstract

Kecamatan Cilongok merupakan salah kecamatan endemis DBD dan pernah mengalami KLB chikungunya. Deteksi virus pada nyamuk sebelum menginfeksi manusia penting sebagai peringatan dini dalam upaya pencegahan wabah di daerah endemis. Tujuan penelitian ini adalah mengetahui infeksi virus Dengue dan Chikungunya pada nyamuk Aedes spp. asal kecamatan Cilongok. Penelitian ini dilakukan di empat desa di Kecamatan Cilongok yang meliputi Desa Cilongok, Pernasidi, Kalisari, dan Jatisaba. Desain penelitian ini adalah studi purposive random sampling . Deteksi virus Dengue dan Chikungunya pada nyamuk dilakukan menggunakan teknik Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR). Hasil positif virus dianalisis secara deskriptif untuk menggambarkan potensi transmisi virus. Hasil penelitian menunjukkan bahwa nyamuk Aedes spp. yang tertangkap tidak mengandung virus Dengue dan Chikungunya. Kata kunci: Aedes spp., chikungunya virus, dengue virus, teknik RT-PCR
The Diversity and Abundance of Termites (Order: Isoptera) at Altitude 200 M ASL Karst Area Southern Gombong, Kebumen Regency nufus, Wafiyyatun; Pratiknyo, Hery; Sukarsa, Sukarsa
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.1.1816

Abstract

Termites are social insects that have a very wide distribution. The termites in the forest ecosystem have many species and feeding habits, it can be said that diversity and abundance determine the role in the termite ecosystem. The termite are very important as a mediator for decomposition of the ecosystem of karst area. Therefeore, the aim of this study was to determine the diversity and abundance of termites in kasrt area at an altitude of 200 m asl Southern Gombong, Kebumen Regency. The study was conducted in a survey method and arranged as follows, in which 20 sub transect size of 5 m x 2 m as repetition and the termite was found is taken to vial bottle with alcohol 70% for identification. The data analysis using F test and continue with the LSD analysis. The study was obtained three diversity of termite in the karst area, there are M. gilvus, M. inspiratus and S. javanicus. The abundance of termite was obtained by M. gilvus is highest than the other termite with percentage of 90% while the abundance of termites based on plot was obtain in plot 1 with percentages 30,4%.
Pemberian Inokulum Fungi Mikoriza Arbuskula (Fma) Campuran terhadap Kemunculan Penyakit Busuk Pangkal Batang Sclerotium pada Tanaman Cabai Rawit dan Cabai Merah Pradana, Wira Dhyaksa; Dwiputranto, Uki; Muljowati, Juni Safitri
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.1817

Abstract

Cayenne pepper (Capsicum frutescens) and red chili (Capsicum annuum), including vegetables and fruit are widely consumed by the public, and also have many benefits. At present, the market demand for cayenne pepper and red chili is very high, so equalization must be made from the production sector. The conventional way of handling such as the administration of pesticides or other chemicals is less effective because it causes side effects that have a large enough impact, so an alternative technique is used that is to use Arbuscular Mycorrhizal Fungi (FMA) thus, research on the administration of Arbuscular Mycorrhoid Fungi Inoculum is thus carried out. (FMA) Mixture of Sclerotium Stem Rot Rotation in Capsicum frutescens and Capsicum annuum. The purpose of this study was to determine the effect of mixed FMA inoculums in suppressing the intensity of sclerotium stem rot rot disease in cayenne pepper (Capsicum frutescens) and red chili peppers (Capsicum annuum) and to determine the optimal dose of mixed AMF in suppressing the intensity of sclerotium stem rot rot on cayenne plants (Capsicum frutescens) and red chili plants (Capsicum annuum). This study used a Completely Randomized Design (CRD) with different doses of mixed AMF inoculums (0, 10, 15, 20, 25 g FMA with zeolite / plant carrier medium). The results of this study indicate that the treatment of mixed AMF inoculums on the intensity of sclerotium stem rot disease in cayenne and red chili plants can reduce the intensity of Sclerotium stem rot disease by 22% and in red chili plants by 11%.
Effect of Batik Wastewater Resulted from Biosorption Process to Blood Glucose Level on Carp (Cyprinus carpio) Oktaviani, Safira Dwi; Lestari, Sri; Wibowo, Eko Setio
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.1819

Abstract

Chromium from the process of coloring batik has high toxicity to the organism. The biosorption method is the heavy metal adsorption process using inactive organisms to eliminate or decrease chromium in batik wastewater. In a polluted environment, fish can be in a stressful condition that requires energy to adapt to environmental changes; high energy requirements will stimulate an increase in blood glucose levels. Chromium exposure can increase the blood glucose level on carp (Cyprinus carpio). This study aims to determine the effect of batik wastewater resulted from the biosorption process exposure to the blood glucose levels. This study used 4 concentrations with 6 replications. Carp is placed in a mixed water tank with batik waste resulting from biosorption. The treatment of batik wastewater produced from the biosorption process concentration are of 0%v.v-1; 39,657%v.v-1; 26,438%v.v-1; and 13,219%v.v-1. Research shows that chromium was decreasing the results obtained at K0 are 68 mg/dL, at K1 is 132 mg/dL, K2 is 127 mg/dL, and at K3 is 116 mg/dL Batik wastewater resulted from biosorption process increasing the blood glucose level in fish and accelerates the rate of expenditure on fish The optimaly concentration of batik wastewater resulted from biosorption process are tried can Keyword : Batik wastewater, Biosorption, Blood glucose level, Carp
Kerapatan dan Distribusi Genus Acanthus pada Area Kerusakan Mangrove di Segara Anakan Bagian Barat Cilacap Nur Azizah, Yasmin Shafira; Ardli, Erwin Riyanto; Yani, Edy
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.1824

Abstract

Mangrove merupakan salah satu ekosistem langka dan khas yang terdapat hanya 2 % di seluruh permukaan bumi. Ekosistem mangrove terluas di dunia berada di Indonesia. Ekosistem mangrove berperan dalam bidang ekologi, ekonomi serta sosial dan budaya. Hutan mangrove setiap tahun terus mengalami penurunan luas dan perubahan tingkat kerapatan akibat kerusakan. Salah satu tumbuhan yang terdapat di ekosistem mangrove adalah genus Acanthus. Genus Acanthus merupakan tumbuhan yang dapat digunakan sebagai bioindikator kerusakan mangrove dan dapat berkembangbiak secara vegetatif dan generatif. Tujuan dari penelitian ini adalah menghitung kerapatan dan distribusi genus Acanthus pada area kerusakan mangrove di Segara Anakan bagian barat Cilacap. Penelitian dilakukan dengan metode survei dengan teknik pengambilan sampel vegetasi mangrove purposive sampling pada 11 stasiun terpilih di Segara Anakan bagian barat Cilacap. Nilai kerapatan dan Pola distribusi genus Acanthus dianalisis menggunakan Surfer 15 untuk mendapatkan pola distribusi genus Acanthus. Nilai kerapatan dan faktor lingkungan dianalisis menggunakan BIOENV dengan Primer 5 kemudian dianalisis secara deskriptif. Nilai kerapatan genus Acanthus pada 11 stasiun terpilih yaitu 15.520 ind.ha-1. Pola distribusi genus Acanthus di Segara Anakan bagian barat Cilacap yaitu pola distribusi mengelompok (aggregate). Kata kunci: Acanthus, Mangrove, Kerusakan, Segara Anakan
DETEKSI MOLEKULER VIRUS DENGUE SEROTIPE 3 PADA NYAMUK Aedes aegypti DI WILAYAH PURWOKERTO TIMUR Prayitno, Priskila Agnesia; Kusmintarsih, Endang Srimurni; Wahyono, Daniel Joko
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.1826

Abstract

Penyakit Demam Berdarah Dengue (DBD) adalah penyakit menular yang disebabkan oleh virus Dengue dan ditularkan melalui gigitan nyamuk Aedes aegypti. Nyamuk tersebut merupakan vektor utama dalam penularan virus Dengue (DENV) dengan ciri khas tubuh dan tungkainya ditutupi sisik dengan garis-garis putih keperakan. Persebarannya luas di daerah tropis dan subtropis, Purwokerto termasuk daerah endemis DBD dan sekaligus ditemukan nyamuk sebagai vektornya. Kecamatan Purwokerto Timur menempati urutan tertinggi dari banyaknya kejadian DBD di wilayah Banyumas, bahkan kejadian luar biasa (KLB) yang terjadi di Kelurahan Sokanegara pada tahun 2016 hingga menyebabkan kematian. Penelitian sebelumnya melaporkan bahwa virus Dengue yang paling banyak ditemukan di Purwokerto adalah serotipe 3. Oleh karena itu, deteksi molekuker nyamuk Ae. aegypti perlu dilakukan berkaitan dengan prediksi penularan Dengue untuk memperoleh informasi awal dalam pencegahan dan pengendalian DENV.Tujuan penelitian ini adalah untuk mendeteksi virus Dengue serotipe 3 pada nyamuk dewasa Ae. aegypti sebagai vektornya. Metode penelitian yang digunakan yaitu metode survei dengan pendekatan secara cross sectional dan teknik pengambilan sampel purposiveAnalisis data survei dilakukan dengan melihat positivitas DENV serotipe 3 pada nyamuk. Hasil penelitian menunjukkan DENVserotipe 3 tidak terdeteksi pada nyamuk yang di sampling di Purwokerto Timur. Kata kunci : Aedes aegypti, Demam Berdarah Dengue, DENV
Epiphytic Orchid Inventory and The Host In Bantarbolang Nature Reserve - Central Java Mawardi, Rahmi Mutia; Herawati, Wiwik; Widodo, Pudji
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.1.1830

Abstract

Bantarbolang Nature Reserve is located in the northern part of Central Java Province. The study aiming to verify the plant species of epiphytic orchids and the host tree in the area of Bantarbolang Nature Reserve was conducted in February to April 2019. The research method used in the research was explorative methods. Data were analyzed descriptively. It was recorded that there were 5 epiphytic orchid species, i.e. Rhynchostylis retusa, Aerides odorata, Cymbidium bicolor, Dendrobium crumenatum and Ascocentrum miniatum. The number of orchids found was 148 individuals, the most common species was Rhynchostylis retusa. The host trees are teak (Tectona grandis), putat (Barringtonia acutangula), bulu (Ficus annulata) and bungur (Lagerstroemia speciosa) with characteristics of hard stems, cracked surface texture, rough, mossy and not easy to peel.
Efek Subletal Limbah Batik Terhadap Aktivitas Enzim Superoksida Dismutase pada Serum Ikan Nila (Oreochromis niloticus) Salmaa, Annisa Nafiah; Hernayanti, Hernayanti; Rachmawati, Farida Nur
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.1.1833

Abstract

Limbah batik merupakan salah satu penyebab pencemaran di lingkungan perairan karena diketahui mengandung beberapa jenis logam berat. Jenis logam berat tersebut antara lain seperti krom (Cr), seng (Zn), merkuri (Hg), tembaga (Cu), timbal (Pb), Arsen (As), kadmium (Cd), mangan (Mn) dan nikel (Ni). Ikan yang terpapar limbah batik memberikan respon secara fisiologis. Respon yang muncul sebelum ikan mengalami kematian disebut efek subletal. Ikatan antara logam berat atau metal (M) dengan protein metalotionin ini disebut sebagai ikatan M+Mt. Ikatan ini bersifat stabil sehingga tidak mudah lepas. Hal inilah yang akhirnya memicu pembentukan radikal bebas. Ketika radikal bebas dalam tubuh semakin meningkat, maka kadar enzim Superoksida Dismutase atau SOD akan semakin menurun. Pengujian kadar enzim SOD dapat dijadikan sebagai upaya deteksi dini adanya pencemaran perairan yang disebabkan oleh limbah batik. Berdasarkan hal tersebut, maka penelitian ini memiliki tujuan untuk melihat pengaruh toksisitas limbah batik terhadap penurunan enzim SOD pada ikan nila. Berdasarkan hasil penelitian ini, kadar SOD mulai dari kontrol, konsentrasi 17% v/v, 34% v/v dan 51% v/v berturut-turut yaitu 29,48 ± 1,40 U/mL, 22,20 ± 1,18 U/mL, 17,77 ± 1,16 U/mL, 13,68 ± 0,56 U/mL. Hasil analisis data menunjukkan bahwa nilai F hitung (218,570) > F tabel (4,30) yang artinya terdapat pengaruh paparan limbah batik terhadap penurunan aktivitas SOD. Semakin tinggi konsentrasi limbah batik maka semakin rendah aktivitas SOD. Kata kunci: Limbah Batik, Oreochromis niloticus, Superoksida Dismutase (SOD)
Deteksi Molekuler Virus Chikungunya pada Nyamuk Aedes aegypti Menggunakan Metode Two Step RT PCR Sativa, Alvira Rifdah; Kusmintarsih, Endang Srimurni; Ambarningrum, Trisnowati Budi
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.1834

Abstract

The Chikungunya fever is a disease caused by an Alphavirus from the family Togaviridae with the symptoms of a patient's posture that is bent over by severe joint pain (arthalgia). Chikungunya disease can be transmitted to humans through an Aedes aegypti as a vector. The outbreak of Chikungunya in Indonesia was first reported in 1973 in Samarinda and then spread to various other regions. Surveillance data show almost every year of the outbreak occurred in various regions in Indonesia. In 2013 there was the outbreak of Chikungunya in North Purwokerto, especially in Bancarkembar and Grendeng.Until now, the medicine or vaccine has not been found to prevent Chikungunya disease. The purpose of this research is to know the infection of virus Chikungunya on Ae. aegypti. This research was conducted by surveying methods with sampling techniques using purposive sampling The observed parameter is th positivity of virus Chikungunya. Analysis data by observing the appearance of DNA band on UV Transilluminator. The results that the amplikon cDNA CHIKV is not detected by the two step RT-PCR method. Key Words : Aedes aegypti, Chikungunya, DNA, Vector
Decolorization Of Indigosol Blue Dye Using Trametes versicolor F200 and Aspergillus sp Pertiwi, Sunu; Dewi, Ratna Stia; Sari, Ajeng Arum
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.1835

Abstract

The dyeing process of batik eventually produces much of wastewater. The difficult degradation and the dangers posed within the synthetic dyes are the main concerns in finding efficient wastewater treatment. Biological treatment has been known to be an effective technique of reducing or eliminating color intensity in wastewater. Fungi is one organism that can decompose many environmental pollutants. The aims of this research were to determine the ability of fungal isolates in decolorizing the synthetic dyes and analyzed which treatment has the highest decolorization percentage. Fungal isolates of Trametes versicolor F200 and Aspergillus sp. were used as a biological agent to decolorize of Indigosol Blue dye. The decolorization percentage was analyzed by spectrophotometer method. The result showed that T. versicolor F200 and Aspergillus sp. able to decolorize Indigosol Blue dye. The decolorization treatment of Indigosol Blue dyes using T. versicolor F200 showed the highest decolorization percentage reaching 97.21%.

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