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Arif Rahman Hikam
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BioEksakta : Jurnal Ilmiah Biologi Unsoed
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 27148564     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
Jurnal BioEksakta menerbitkan artikel ilmiah hasil penelitian dalam bidang biologi umum termasuk Ekologi dan konservasi, Taksonomi dan Struktur, Biogeografi, Evolusi, Biodeversitas, Fisiologi dan Reproduksi, Biologi sel, Biologi Molekuler dan Genetika.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 379 Documents
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Karakterisasi Isolat Khamir Gf1 Terhadap Kondisi Asam Saluran Pencernaan Unggas Awwaluz Zahroh Mahya Ainillah; Nuraeni Ekowati; Ade Erma Suryani
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.833 KB) | DOI: 10.20884/1.bioe.2020.2.1.1745

Abstract

Probiotik merupakan mikroorganisme yang dapat memperbaiki mikroekologi usus yang berdampak positif terhadap kesehatan inang. Kandidat probiotik merupakan mikroorganisme yang memiliki potensi sebagai probiotik, seperti menghasilkan enzim fitase sehingga mampu mengoptimalkan kondisi saluran pencernaan. Salah satu mikroorganisme sebagai kandidat probiotik adalah khamir penghasil enzim fitase. Khamir penghasil fitase telah diisolasi oleh BPTBA-LIPI Yogyakarta asal makanan fermentasi tradisional Indonesia yaitu gathot.Pengujian khamir fitase sebagai kandidat probiotik dilakukan dengan menstimulasikan kondisi saluran pencernaan seperti kondisi asam yaitu pada pH rendah dan kondisi asam lambung. Kondisi pH yang rendah dan asam lambungmerupakan suatu penghalang masuknya mikroorganisme ke saluran gastrointestinal, oleh karena itu pengujian terhadap asam dilakukan untuk mengetahui karakteristik mikroorganisme yang akan digunakan sebagai kandidat probiotik. Pengujian dilakukan secara invitro dengan menguji ketahanan khamir pada kondisi asam dengan nilai pH 2 dan 3 serta kondisi asam lambung dengan menggunakan pepsin 0,3%. Hasil penelitian menunjukkan bahwa isolat khamir GF1 asal makanan tradisional fermentasi mampu tahan terhadap kondisi asam karena mampu mempertahankan viabilitasnya pada pH 3 dan kondisi asam lambung.
Kerapatan dan Distribusi Genus Acanthus pada Area Kerusakan Mangrove di Segara Anakan Bagian Barat Cilacap Yasmin Shafira Nur Azizah; Erwin Riyanto Ardli; Edy Yani
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.187 KB) | DOI: 10.20884/1.bioe.2020.2.2.1824

Abstract

Mangrove merupakan salah satu ekosistem langka dan khas yang terdapat hanya 2 % di seluruh permukaan bumi. Ekosistem mangrove terluas di dunia berada di Indonesia. Ekosistem mangrove berperan dalam bidang ekologi, ekonomi serta sosial dan budaya. Hutan mangrove setiap tahun terus mengalami penurunan luas dan perubahan tingkat kerapatan akibat kerusakan. Salah satu tumbuhan yang terdapat di ekosistem mangrove adalah genus Acanthus. Genus Acanthus merupakan tumbuhan yang dapat digunakan sebagai bioindikator kerusakan mangrove dan dapat berkembangbiak secara vegetatif dan generatif. Tujuan dari penelitian ini adalah menghitung kerapatan dan distribusi genus Acanthus pada area kerusakan mangrove di Segara Anakan bagian barat Cilacap. Penelitian dilakukan dengan metode survei dengan teknik pengambilan sampel vegetasi mangrove purposive sampling pada 11 stasiun terpilih di Segara Anakan bagian barat Cilacap. Nilai kerapatan dan Pola distribusi genus Acanthus dianalisis menggunakan Surfer 15 untuk mendapatkan pola distribusi genus Acanthus. Nilai kerapatan dan faktor lingkungan dianalisis menggunakan BIOENV dengan Primer 5 kemudian dianalisis secara deskriptif. Nilai kerapatan genus Acanthus pada 11 stasiun terpilih yaitu 15.520 ind.ha-1. Pola distribusi genus Acanthus di Segara Anakan bagian barat Cilacap yaitu pola distribusi mengelompok (aggregate). Kata kunci: Acanthus, Mangrove, Kerusakan, Segara Anakan
Biodegradasi Limbah Lindi Hitam, Acid Orange dan Acid Red menggunakan Jamur Trametes versicolor F200 Rismi Seftiani Jaitun; Nuraeni Ekowati; Ajeng Arum Sari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.789 KB) | DOI: 10.20884/1.bioe.2019.1.2.1806

Abstract

Limbah lindi hitam dan zat warna azo (acid orangedan acid red) merupakan limbah berbahaya yang dapat mengkontaminasi ekosistem, sehingga perlu dilakukan pengolahan sebelum limbah dibuang ke ekosistem. Pengolahan secara fisik maupun kimiawi memiliki kelemahan diantaranya membutuhkan input energi tinggi, biaya yang mahal, penerapan yang terbatas dan menghasilkan produk lain yang bersifat toksik. Trametes versicolor dapat dijadikan sebagai agen biodegradasi, sebab menghasilkan anzim ligninolitik seperti Lakase (Lac), Manganese peroksidase (MnP) dan Lignin peroksidase (LiP).Jamur T. versicolor F200 mampu mendekolorisasi hingga 50%pada limbah lindi hitam; 90% pada Acid orange; dan 90% pada Acid red. Aktivitas enzim Lac, MnP dan LiP terbaik masing-masing yaitu 143,59 U/L (6 hari inkubasi), 33,3 U/L (6 hari inkubasi) dan 1167,42 U/L (awal inkubasi) pada limbah lindi hitam; 520,513 U/L) (awal inkubasi), 138 U/L (awal inkubasi) dan 358 U/L (awal inkubasi) pada acid orange; dan 408,3 U/L (awal inkubasi, 77 U/L (awal inkubasi) dan 228,871 U/L (akhir inkubasi). Kata kunci : Biodegradasi, Limbah lindi hitam, Trametes versicolor F200 dan acid orange dan acid red
MORFOLOGI KEPITING Albunea symmysta (ALBUNEIDAE:CRUSTACEAE) DARI PANTAI PARANGKUSUMO YOGYAKARTA Dyah Purry; Dian Bhagawati; Agus Nuryanto
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (410.431 KB) | DOI: 10.20884/1.bioe.2020.2.1.1842

Abstract

Familia Albuneidae terdiri atas Sembilan Genus dan salah satunya yaitu Albunea. Distribusi Albunea di Indonesia telah dilaporkan oleh beberapa peneliti terdahulu, diantaranya terdapat di pesisir Cilacap, Kebumen, Yogyakarta, Aceh, dan Bengkulu. Berdasarkan hasil survei pendahuluan yang dilakukan di Pantai Parangkusumo Yogyakarta telah ditemukan kepiting yang memiliki morfologi hampir sama dengan dengan spesies Albunea symmysta yang pernah ditemukan sebelumnya di lokasi yang berbeda. Atas dasar hal tersebut maka telah dilakukan kajian untuk mengetahui kepastian nama spesiesnya. Penelitian ini bertujuan untuk mengidentifikasi kepiting Albunea yang dimiliki oleh yang berasal dari pantai Parangkusumo Yogyakarta serta untuk mengetahui semua karakter performa dan meristik milik kepiting Albunea yang berasal dari pantai Parangkusumo. Metode penelitian yang digunakan adalah survei dan pengambilan sampel secara purposive random sampling. Sampel dari pantai Parangkusumo diambil sebanyak dua kali dengan interval waktu dua minggu, pengambilan sampel dilakukan hingga memperoleh jumlah sampel sebanyak 60 ekor. Identifikasi dilakukan berdasarkan karakter morfologi, yang meliputi performa, morfometri dan meristik. Analisis data dilakukan secara deskriptif untuk performa dan meristik, sedangkan morfometrik dengan uji T. Berdasarkan pengamatan performa morfologi, meristik, hasil penelitian yang didapat yaitu kepiting Albunea yang diperoleh dari Parangkusumo Yogyakarta adalah spesies Albunea symmysta yang memiliki karakter karapas dengan bentuk hampir empat persegi panjang, memiliki antenula yang sangat panjang, karapas dengan permukaan rata, memiliki dactylus pertama subchelate, memiliki 9-12 buah duri anterolateral dan memiliki tujuh buah segmen flagella antena
Pengaruh Paklobutrazol dan Ga3 Terhadap Pertumbuhan dan Pembungaan pada Tanaman Tomat (Solanum lycopersicum) Nikolaus Widyasmara; Rochmatino Rochmatino; Lucky Prayoga
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.128 KB) | DOI: 10.20884/1.bioe.2019.1.2.1787

Abstract

Tomato is a horticultural commodity that has many benefits, it not only functions as a fruit vegetable but is also often used as a complement to spices, food coloring ingredients and fresh drinks because of its nutritional content. To increase tomato production, paklobutrazol is needed. Paklobutrazol has a function to inhibit the biosynthesis of gibberellins, so that the administration of these substances causes stunting elongation and stimulates flower induction. While GA3 serves to encourage the development of seeds, elongation of stems, encourage flowering and fruit development. This research was conducted with the aim of assessing the effect of Paklobutrazol and GA3 on growth and flowering on tomato plants and determine the concentration of Paklobutrazol and GA3 which are appropriate for the growth and flowering of tomato plants. This research was carried out experimentally using a completely randomized design (CRD) 2 factor factorial pattern. Each treatment combination was repeated 3 times so that there were 48 experimental units. The concentrations of paklobutrazol used were 0 ppm, 250 ppm, 500 ppm, and 750 ppm, while the concentration of GA3 used was 0 ppm, 50 ppm, 100 ppm and 150 ppm. The parameters observed in this study were plant height (cm), flowering time (days), and number of flowers (fruit). The data obtained were analyzed by Analysis of Variance with a confidence level of 95%, and continued with the Smallest Significant Difference test (LSD) at an error rate of 5%. The results showed that administration of paklobutrazol had an effect on inhibiting the height of tomato plants, and GA3 had an effect on accelerating flowering of tomato plants. Paklobutrazol with a concentration of 750 ppm is the best concentration to inhibit plant height, and GA3 with a concentration of 150 ppm is the best concentration to spur tomato flowering.
Pengaruh Asam Humat pada Media Tanam Zeolit terhadap Pertumbuhan dan Kandungan Vitamin C Sawi Hijau (Brassica juncea) Istiq Fauziah; Elly Proklamasiningsih; Iman Budisantoso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.186 KB) | DOI: 10.20884/1.bioe.2019.1.2.1669

Abstract

Green mustard (Brassica juncea) as one of the commonly consumed vegetables and acts as one of the main sources of C vitamin in addition to fruits. C vitamin is needed for the body because it has antioxidant. Humic acid is a component of organic acid soil which can increase the production of food crops and plantations. Zeolites have a hollow structure that allows use as a carrier of humic acid. Aside from being a career zeolite which is applied with humic acid can act as an ameliorant material, this is useful in increasing crop production. The objectives of this study are to determine the effect of humic acid with zeolite planting media on the growth and content of C vitamin on green mustard plants and determine the concentration of humic acid which most influences the growth and content of C vitamin in green mustard plants. The research was conducted at the Laboratory of Plant Physiology and Green House, Faculty of Biology, Jenderal Soedirman University, Purwokerto using an experimental method. The study was carried out from January to February 2019 using an experimental design namely Completely Randomized Design (CRD). The parameters were the number of leaves, wet weight and dry weight of roots, stems, leaves and C vitamin. The data obtained will be analyzed by analysis of variance at the confidence level of 95% and 99%, if the results are significantly different then continued with LSD at 95%. The results showed that application of humic acid in zeolite planting media had a very significant effect on growth and C vitamin content of green mustard greens. Humic acid with a concentration of 4 g.kg-1 is the most efficient concentration in increasing growth and C vitamin content of green mustard greens.
Pemberian Inokulum Fungi Mikoriza Arbuskula (FMA) Campuran terhadap Kemunculan Penyakit Layu Fusarium pada Tanaman Semangka [Citrullus lanatus (Thunb.) Matsum. & Nankai] Berbiji dan Non Biji Riska Febriyana; Uki Dwiputranto; Endang Sri Purwati
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.988 KB) | DOI: 10.20884/1.bioe.2020.2.2.2006

Abstract

Watermelon (Citrullus lanatus) is a plant originating from the dry region of North Africa and is now cultivated in almost all regions of the world as a fruit that has high economic value. Efforts to cultivate and breed watermelons become very important related to defense against disease. One of the dominant diseases in watermelon is fusarium wilt disease caused by Fusarium oxysporum. Mycorrhiza is a symbiotic mutualism between certain fungi and higher plants. Arbuscular mycorrhizal fungi (AMF) can be used as an alternative in reducing fusarium wilt effect. The purpose of this research is to know the effect and effective dose of AMF mixture inoculum to reduce Fusarium wilt disease in seeded and seedless watermelon. This research used a completely randomized design (CRD) with different doses of mixed AMF inoculums (0, 5, 10, 15, 20 g) AMF with zeolite/plant carrier medium. The main parameters discussed are the disease intensity and disease incubation period, while the supporting parameters observed were pH, temperature, humidity, and degree of infection. The data obtained were analyzed using Variance Test (F test) with a Standard Error of 5%. The results of this research shows that there is effect of inoculation AMF mixture inoculum to reduce Fusarium wilt disease in seeded and seedless watermelon. The effective dose of AMF mixture to reduce the Fusarium wilt disease in seeded watermelon is inoculation AMF mixture dose 10 g / plant and inoculation AMF mixture dose 15 g/ plant in seedless watermelon.
Reverse Transcriptase PCR (Rt-PCR) for Detection of Dengue and Chikungunya Virus of Mosquito Aedes aegypti in Sokaraja Dyah Retno Annisa; Endang Srimurni Kusmintarsih; Trisnowati Budi Ambarningrum
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.337 KB) | DOI: 10.20884/1.bioe.2020.2.1.1811

Abstract

dengue fever (DF). Meanwhile, chikungunya virus causes Chikungunya fever (CF). These diseases involve three organisms, namely virus, mosquito Aedes sp., and human. The transmission of dengue and chikungunya virus is related to the population of Ae. aegypti. Banyumas regency is one of the regions with many cases of dengue and chikungnya virus infections, particularly in Purwokerto, Sokaraja, and Cilongok sub-district. Up to this time, there is no medicine and vaccine provided to treat these viruses effectively. Thus, detection of virus inside vector will be effectively performed in order to predict the transmission risk of dengue and chikungunya virus. This research aimed to know the molecular detection of dengue and chikungunya virus on adult Ae.aegypti mosquito in Sokaraja Region, Banyumas Regency. Survey was done by a cross-sectional method in Sokaraja sub-district from May 2019 – March 2019. Furthermore, technical sampling that used was purposive sampling method of adult Ae.aegypti using BG-Sentital Trap, followed by molecular detection of dengue virus using Two-step RT-PCR and chikungunya gene virus using RT-PCR. Molecular detection of DENV and CHIKV of mosquitoes which collected from Sokaraja region showed negative result
Pemberian Inokulum Fungi Mikoriza Arbuskula (Fma) Campuran terhadap Kemunculan Penyakit Busuk Pangkal Batang Sclerotium pada Tanaman Cabai Rawit dan Cabai Merah Wira Dhyaksa Pradana; Uki Dwiputranto; Juni Safitri Muljowati
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (455.187 KB) | DOI: 10.20884/1.bioe.2020.2.2.1817

Abstract

Cayenne pepper (Capsicum frutescens) and red chili (Capsicum annuum), including vegetables and fruit are widely consumed by the public, and also have many benefits. At present, the market demand for cayenne pepper and red chili is very high, so equalization must be made from the production sector. The conventional way of handling such as the administration of pesticides or other chemicals is less effective because it causes side effects that have a large enough impact, so an alternative technique is used that is to use Arbuscular Mycorrhizal Fungi (FMA) thus, research on the administration of Arbuscular Mycorrhoid Fungi Inoculum is thus carried out. (FMA) Mixture of Sclerotium Stem Rot Rotation in Capsicum frutescens and Capsicum annuum. The purpose of this study was to determine the effect of mixed FMA inoculums in suppressing the intensity of sclerotium stem rot rot disease in cayenne pepper (Capsicum frutescens) and red chili peppers (Capsicum annuum) and to determine the optimal dose of mixed AMF in suppressing the intensity of sclerotium stem rot rot on cayenne plants (Capsicum frutescens) and red chili plants (Capsicum annuum). This study used a Completely Randomized Design (CRD) with different doses of mixed AMF inoculums (0, 10, 15, 20, 25 g FMA with zeolite / plant carrier medium). The results of this study indicate that the treatment of mixed AMF inoculums on the intensity of sclerotium stem rot disease in cayenne and red chili plants can reduce the intensity of Sclerotium stem rot disease by 22% and in red chili plants by 11%.
Toksisitas Subletal Limbah Cair Batik Hasil Biosorpsi Terhadap Struktur Histologis Ginjal Ikan Mas (Cyprinus carpio) Khalil Ibrahim Sani; Sri Lestari; Atang Atang
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.261 KB) | DOI: 10.20884/1.bioe.2020.2.1.1769

Abstract

Histopathological studies related to the kidney of fish exposed to batik wastewater resulted from the biosorption process are necessary because the kidney is an target organ that plays an important role in maintaining water balance and osmoregulation. This research aims to determine the effect of batik wastewater resulted from the biosorption process exposure on kidney histology of Carp (Cyprinus carpio) and determine the concentration that has the most effect on it. The treatment consists of K0 (control) and three treatments of batik wastewater resulted from the biosorption process with different concentration from 25 %, 50 %, and 75 % LC50 96h, ie K1 1,32; K2 2,64, and K3 3,96 % v.v-1. The carp were exposed to batik wastewater resulted from the biosorption process for 7 days, and on the 8th day the fish was dissected, and the kidney was taken for histology process. The result of the research shows that exposure to batik wastewater resulted from the biosorption process with different concentrations give negative impact on the carp kidney such as tubular hypertrophy, tubular necrosis, and glomerular necrosis.

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