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HAYATI Journal of Biosciences
ISSN : 19783019     EISSN : 20864094     DOI : -
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
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Articles 1,091 Documents
Gold Mine Wastewater Induced Morpho-physiological Alteration of Four Biodiesel Producing Species Hamim Hamim; Lutfiani Lutfiani; Deden Saprudin; Putri Nur Azizah Hutagalung; Hadisunarso Hadisunarso; Luluk Setyaningsih; Hirmas Fuady Putra
HAYATI Journal of Biosciences Vol. 28 No. 1 (2021): January 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.1.63

Abstract

This experiment aimed to determine the adaptability of four biodiesel-producing plants to gold mine wastewater based on morpho-physiological properties. Four species namely jatropha (Jatropha curcas), castor bean (Ricinus communis), wild candlenut (Reutealis trisperma) and bead tree (Melia azedarach) were grown in water culture using Hoagland solution for 2 weeks and then treated with gold mining wastewater at 0% (control), 25%, 50%, and 75% (v/v) for 3 weeks. Growth, anatomical and physiological characteristics were observed during the treatment. The results showed that gold mine wastewater significantly decreased growth of the four species despite varied among the species. Wastewater had significant effect on leaves anatomy of Jatropha but not on others. It also significantly induced the increase of malondialdehyde (MDA) content in Jatropha and castor bean, but not in wild candlenut and bead-tree. Meanwhile, the treatment significantly decreased chlorophyll content of all species with the most in bead-tree plant, and leaves relative water content (RWC) particularly in castor bean and bead-tree plants. There was strong negative correlation between the increase of MDA content and the decrease of chlorophyll content and leaf RWC. Among the four species, wild candlenut (R. trisperma) was the most resistant to gold mine wastewater based on morphological and physiological properties.
Paddy Loss and Its Implication to Fertilizer Subsidy in Indonesia A Faroby Falatehan; Yusman Syaukat; Hastuti Hastuti; Nizar Nasrullah
HAYATI Journal of Biosciences Vol. 28 No. 1 (2021): January 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.1.73

Abstract

Indonesia is the third largest rice producer in the world, at the same time it’s a country that imports rice from various countries. The government has provided input subsidies to increase rice production and reduce imports. Modes of rice farming in Indonesia is relatively diverse, from conventional to modern, even in the post-harvest process. The objectives of this study are: to analyze the impact of post-harvest handling on food loss and to analyze the relationship between paddy or rice loss with the quantity and value of fertilizers subsidy and paddy production in Indonesia. The estimated food (rice) loss includes the stages of harvesting, threshing, drying, and milling stages and distribution. Farmers use various technologies in processing rice: serrated sickle in harvesting, power thresher in threshing, and flatbed dryer in drying; while rice milling was done using conventional rice miller. The total rice loss reached 6.91 million tons in 2014 and continued to increase to 8.14 million tons in 2018. The growth rate of fertilizer subsidy value is higher than those of subsidized fertilizer quantity and paddy or rice loss. The results indicated that growth rates of paddy production and rice loss was lower than increased of government subsidy for fertilizer.
Designing Wildlife Corridor Along Cikapundung River in Bandung Urban Area (Indonesia) based on Comparation with Kamo River in Kyoto (Japan) Evita Izza Dwiyanti; Shozo Shibata; Ryo Nukina; Tien Lastini; Endang Hernawan
HAYATI Journal of Biosciences Vol. 28 No. 1 (2021): January 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.1.83

Abstract

Cikapundung is a river connecting wildlife habitat in two open green space areas; there are Tahura Djuanda and Bandung Zoo. Now, these habitats are fragmented due to intervention by urban activities in the Cikapundung River boundary. One of the proposed solutions is to develop a wildlife corridor along the Cikapundung River. The purpose of this study is to identify the landcover of the Cikapundung River, analyze the potential area which can be developed into a wildlife corridor, designing the landscape of the corridor tailored to the needs of the wildlife, and comparing the condition of the Cikapundung River boundary with Kamo River in Kyoto, Japan. Primary data is landcover of Cikapundung River boundary obtained through on-screen digitizing from satellite imagery using ArcMap. Secondary data are the biodiversity list in Cikapundung River riparian area and its ecological description. Cikapundung River border has several land cover types; the highest percentage of the landcover is tree canopy 62.2%, followed by buildings 31.0%. As a result of the comparison Cikapundung River has a more significant vegetation species; however, Kamo River has a more significant number of wildlife species. In the design planning, 14 vegetation species have been selected according to 26 target wildlife species' needs.
In-Silico Design of Novel Glucagon-Like Peptide 1 Mutants as Candidate for New Peptide Agonist Drugs Tony Sumaryada; Ajeng Widya Roslia; Alfi Afifah; Setyanto Tri Wahyudi; Agus Kartono
HAYATI Journal of Biosciences Vol. 28 No. 1 (2021): January 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.1.92

Abstract

The binding of glucagon-like peptide 1 (GLP-1) incretin hormone and its receptor GLP-1R plays an important role in the human body. The GLP-1 acts as the insulin secretion stimulator through a GLP-1R agonist activation to avoid the type 2 diabetes mellitus problem. A recent development in computational sciences has enabled us to design a new GLP-1 mutant which has a better binding stability to GLP-1R. In this paper, we have conducted an in-depth analysis of protein-protein docking of GLP-1 and GLP-1R receptor to determine the responsible factors affecting the binding stability. The protein-protein binding stability was analyzed by performing the point mutations on the GLP-1 structure and running the molecular dynamics simulation of the docked structures. Five mutants, Lys20Arg, Lys20His, Lys20Ser, Lys20Gly, and Lys20Ala, has been created computationally and docked with GLP-1R and tested via a molecular dynamics simulation and the free energy perturbation calculation to search for the best-binding mutant. Our results have shown that the Lys20His mutant design has the best potential to be developed as a new peptide agonist drug based on its binding affinity and structural integrity as compared to other mutants and the peptide agonist drugs available in the market exenatide, and liraglutide.
Grain Quality of Rice (Oryza sativa L.) ‘Menthik Wangi’ of Organic Farming Yields Dwi Umi Siswanti; Maryani Maryani; Fitria Yulia Rachmawati; Aprida Niken; Rega Virgiyana Agustin; Niken Wulansari
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.105

Abstract

Organic rice is believed to have better nutrition than conventional rice. Not all organic rice grain is consumed. Most of the organic cultivation yields will be used as seed. Viability test is one of the quality test of rice seed. Viability is seed vitality that can be shown as a physiological and biochemical phenomenon. Seed that have high vigor will achieve high productivity levels as well. The aims of this research was to analyze the quality of rice cultivar Menthik Wangi of organic farming yields. The quality of rice seeds was tested by measuring the viability of seed and crude fat content. The test results demonstrate the viability of the grain has a 100% germination, 100% growth potential maximum, 99.33%, simultaneity grew, 97.33% vigor index, T50 2.33, and seed moisture content 10.67%. The fat content was conducted by soxhlet method and analyzed by T-test. The results showed that organic rice has a higher fat content (0.53%) than non-organic rice (0.24%). It can be concluded that rice seed cultivar Menthik Wangi of organic farming has a higher viability and fat content than the non-organic rice grain.
The Development of In Vitro Culture Sterilization Method of Gametophyte Explant Lopholejeunea sp. Anna Widyastuti; Afiatry Putrika; Astari Dwiranti; Andi Salamah; Niarsi Merry Hemelda; Windri Handayani
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.110

Abstract

In vitro cultures of leafy liverworts are still facing significant challenges due to high-level of explant contamination. The sterilization process can easily damage the structure of liverwort after exposure to the disinfectant. This study was to determine the concentration and time exposure of commercial bleach as a disinfectant to suppress contamination using the gametophyte culture of Lopholejeunea sp. The experiment consisted of control and six treatment combinations of commercial bleach with concentration 0.5, 0.75, and 1% (v/v), and exposure time (60 and 90 seconds). The type and location of contamination, the color of the explants after sterilization, and response after 30 days were observed. The results showed that the 0.75% bleach with 60 and 90 seconds exposure time had a lower contamination until the 7th day of culture. The most common type of contamination is bacteria and fungi that arise from the explant. Despite the contamination, it did not inhibit shoot formation. Further studies still needed to determine the type of fungicides and antibiotics with the most potent concentration and exposure time should be tested to obtain an axenic and viable culture of liverworts Lopholejeunea sp.
Economic Valuation of Mangrove Ecosystem at Gerung District, West Lombok Regency Mufti Petala Patria; Siti Dian Rosadi; Nisyawati Nisyawati
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.118

Abstract

Research regarding economic valuation of mangrove in Gerung District, West Lombok Regency has been conducted. The purposes of this research were to calculate and analyze economic value from mangrove ecosystem at Gerung District. Research methods used observation and interviews. Direct Economic Value (DEV) were obtained from direct use of mangrove with a value IDR. 227,040,000/year. Indirect Economic Value (IEV)were calculated from mangrove ecosystem services with a value IDR. 1,405,041,200/year, Existence Value (EV) was obtained by Contingent Valuation Method (CVM) with a value IDR. 1,520,000/year. Option Value (OV) were calculated from biodiversity value with a value were IDR. 1,200,000/year. Total Economic Value (TEV) of mangrove in Gerung District were IDR. 1,634,801,200/year.
OsDREB2A Gene Polymorphism Does Not Affect Salinity Tolerance Potency of Local Rice Varieties from Banten ‘Ainun Sayyidah Zakiyah; Elhah Nailul Khasna; I Kade Karisma Gita Ardana; Choirun Nita Fikriani; Nina Bunga Anggraini; Dwita Novitasari; Dwi Listyorini; Suharti Suharti
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.123

Abstract

Indonesia possesses vast coastal agricultural land areas with high salinity. This high salinity is a threat to rice productivity which may decrease to 79.8%. Banten province of Indonesia houses many local rice varieties which have salinity tolerance potency based on its agronomical characters. The adaptability of rice plant in high salinity soil can be affected by OsDREB2A gene, which encodes transcription factors from several salt-tolerant signaling pathway genes. The aims of this study were to find out the possible polymorphism of OsDREB2A gene from Banten local rice varieties. The polymorphism of OsDREB2A analyzed by Single Nucleotide Polymorphism (SNP) and possible molecular structure. OsDREB2A gene was amplified using DNA from Tambleng, Bulu Putih, and Pare Caok varieties as templates through PCR method with a pair of specific primers. DNA fragments obtained were analyzed by means of series of analysis software. From those analysis we obtained 840 bp, 836 bp, and 850 bp gene fragments from Tambleng, Bulu Putih, and Pare Caok varieties, respectively. There is polymorphism of OsDREB2A due to some mutation yet does not massively alter its protein structure. From this study, we suggest that Tambleng, Bulu Putih, and Pare Caok varieties potential for salinity stress resistance genetically.
Identification and Histological Observation of Endophytic Fungi Isolated from Medicinal Plant, Physalis angulata L. Utami Sri Hastuti; Sulisetijono Sulisetijono; Dwi Rahmawati; Ria Yustika Sari; Sugi Hartono; Chomisatut Thoyibah; Fitria Maulita; Faiza Nur Imawati Ningsih; Siti Hartina Pratiwi
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.130

Abstract

Some endophytic fungi species live in medicinal plant tissue and does not make any damage, but live in symbiotic mutualism relationship with the host plant. This research was done to: 1) identify the endophytic fungi species isolated from P. angulata leaf, twig, and stem bark tissues, 2) determine the endophytic fungi colonization in the P. angulata plant tissue by histologic observation. The endophytic fungi was isolated from healthy P. angulata plant parts, then inoculated on Potato Dextrose Agar medium and incubated in 27°C for 7-14 days. Each endophytic fungi isolates were identified. The histologic observation was done by microscopic observation to determine the endophytic fungi position in the plant tissue. The conclusion are: 1) seven endophytic fungi species were found: Penicillium verrucosum, Colletotrichum alienum, Fusarium subglutinans, Aspergillus nidulans, Mycelia sterilia 1, Mycelia sterilia 2, and Rhizoctonia sp.; 2) the endophytic fungi micelium was found on the leaf epidermis cell wall, on the twig epidermis cell wall, and parenchyma cell wall, on the stem bark epidermis cell wall. The suggestion of the study: it is need to make the next research about secondary metabolites content produced by endophytic fungi species isolated from P. angulata and their antimicrobial activity.
Identification of Potential Bacteria on Several Lakes in East Java, Indonesia Based on 16S rRNA Sequence Analysis Achmad Rodiansyah; Ainul Fitria Mahmudah; Mastika Marisahani Ulfah; Uun Rohmawati; Dwi Listyorini; Eko Agus Suyono; Sitoresmi Prabaningtyas
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.136

Abstract

Four bacterial isolates from Ranu Pani and Ranu Grati in east java had been revealed to be potentials to produce IAA (PIS isolate), phosphate solubilizer (GPS isolate), cellulose hydrolysis (PSS isolate) and, amylum hydrolysis (PAS), two dominant bacterial isolates from Rani Pani (PØD isolate) and Ranu Grati (GØD isolate) which were co-cultured with microalgae promoted microalgae growth, yet its taxonomical position has not been clearly known. The aim of this study was to identify those bacterial isolates using 16S rRNA barcode. This research conducted by gDNA isolation, the 16S rRNA sequence was amplified using 27F and 1492R primers. Reconstructed phylogenetic trees and genetic distance analysis showed that the isolate PIS and PSS identified as Bacillus cereus Group closely related to Bacillus paramycoides. PAS isolate identified as Bacillus subtilis Group closely related to Bacillus amyloliquefaciens, GPS isolate identified as novel species in genus Enterobacter, and two dominant isolates (PØD and GØD) identified as Enterobacter cloacae complex closely related to Enterobacter cloacae. The genomic approach and additional phenotypes-examination are required to clarify its taxonomical position.

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