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INDONESIA
HAYATI Journal of Biosciences
Published by Institut Pertanian Bogor
ISSN : 19783019     EISSN : 20864094     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
Arjuna Subject : -
Articles 1,091 Documents
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Tempe and Soybean var. Grobogan-Indonesia Increased The Number of Osteoblasts and Osteocytes, Inhibited Osteoclast Damage in The Tibia Bone of Rats Tutik Wresdiyati; Alamsah Firdaus; Made Astawan
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.144

Abstract

Tempe, an Indonesian traditional food, is a product of processed soybeans by fermentation. Both tempe and soybeans contain a variety of nutrients, such as isoflavone, Ca, P, and Mg that are important for bone health. This study aimed to observe the influence of Indonesian var. Grobogan tempe flour and soybean flour on the profile of osteoblasts, osteocytes, and osteoclasts in the tibia bone of rats. The rats were divided into five groups; casein, tempe flour 10%, tempe flour 20%, boiled soybean flour 10%, and boiled soybean flour 20% groups. The treatment was conducted for 90 days. The results showed that the treatment using tempe flour and soybean flour increased the number of osteoblasts and osteocytes significantly compared to the casein group. Tempe flour 20% showed the best result. In addition, the number of osteoclasts in the tibia bone of rats that were treated with tempe flour and soybean flour were smaller than that of negative control group. The treatment using soybean flour (10% and 20%) or tempe flour (10% and 20%) decreased the number of osteoclasts in the tibia bone of rats. It is suggested that both tempe and soybeans can be utilized for osteoporosis prevention.
Endophytic Fungi from Four Indonesian Medicinal Plants and Their Inhibitory Effect on Plant Pathogenic Fusarium oxysporum Nampiah Sukarno; Rohani Cinta Badia Ginting; Utut Widyastuti; Latifah Kosim Darusman; Sihegiko Kanaya; Irmanida Batubara; I Nyoman Pugeg Aryantha; Mashuri Waite
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.152

Abstract

The medicinal plants Centella asiatica, Curcuma xanthorrhiza, Guazuma ulmifolia, and Hydrocotyle verticillata are widely used in Indonesian traditional medicine, but little is known about their associated endophytic fungi. This research aimed to study the diversity of endophytic fungi derived from functional parts of these plants and to evaluate their potential as antifungal agents against the plant pathogenic fungus Fusarium oxysporum. A total of 17 isolates of endophytic fungi were obtained: nine from leaves of G. ulmifolia, three each from leaves of C. asiatica and H. verticillata, and two from rhizomes of C. xanthorrhiza. The genus Colletotrichum was found in all plants studied, but each plant was associated with different species. Colletotrichum aeschynomenes was associated with C. xanthorrhiza, C. siamense was associated with C. asiatica, and C. tropicale was associated with G. ulmifolia and H. verticillata. The species Curvularia affinis, Diaporthe tectonae, Lasiodiplodia mahajangana, Parengyodontium album, Talaromyces trachyspermus, and Speiropsis pedatospora were found only in G. ulmifolia; while Didymella coffeae-arabicae and Muyocopron laterale were found only in H. verticillata. The endophytic fungi showed inhibition activity against F. oxysporum with inhibition values of 6.0-78.9%, T. trachyspermus JBd10 and C. affinis JBd14 gave the highest inhibition activity.
In Vitro Germination and Flowering of Dendrobium capra J.J. Smith, An Endemic Orchid of Java Muhammad Dylan Lawrie; Zulfa Layina; Della Rosiana Ningtias; Falah Nur Alifianto; Ari Indrianto; Aziz Purwantoro; Endang Semiarti
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.172

Abstract

Dendrobium capra is an Indonesian endemic orchid species that live in Java. It grows on low altitude with warm climate. D. capra has beautiful small yellow greenish flower that grow in raceme inflorescence. This orchid faces a threat in its natural habitat due to having a long life cycle and a forestry main commodity as a main host thus categorized as Appendix II on CITES list. To address that problem, ex situ conservation approach using in vitro culture method is necessary. Germination enhancement effort using complex organic substances found that 200 ml/l tomato extract gave best germination result. Analysis on D. capra plantlet growth also showed that MS medium produced better plantlet size than NP, VW and KC medium. Supplementing medium with a combination of NAA and TDZ has also successfully induced early flowering within 11 month of culture period. This information is important to achieve successful in vitro culture of D. capra for various purposes.
Analysis of Genetic Potential of Banyuwangi Local Rice (Oryza sativa L.) Based on Relative Expression of Homogentisate Geranylgeranyl Transferase (HGGT) and Granule-Bound Starch Synthase I (GBSSI) Gene Dyati Galuh Pratita; Tita Putri Milasari; Elhah Nailul Khasna; Sunarmi Sunarmi; Evi Susanti; Suharti Suharti; Delia Wahyu Pangesti; Annasa Sabatia; Dwi Listyorini
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.181

Abstract

Rice nutrition including vitamin and amylose contents become important aspect for many people around the world. Rice with high amylose content (low glycemic index) is good for those with Diabetes mellitus. Tocotrienol, one precursor of Vitamin-E biosynthesis is catalyzed by enzymes encoded HGGT, while amylose biosynthesis is catalyzed by enzymes encoded GBSSI. The objective of this study was to find rice varieties with high tocotrienol and/or amylose content based on the expression of HGGT and GBSSI among eight Banyuwangi local rice varieties. Relative expression of HGGT and GBSSI was measured by qRT-PCR and analyzed using 2ΔCt method. Statistical analysis resulted in the significantly different of HGGT and GBSSI relative expression among samples. Relative expression of HGGT from the highest to the lowest were demonstrated by Hitam Melik, Hitam Pekat, Blambangan A3, Merah Bali, Blambangan A2, Berlian, Janur Kuning, and SOJ A3, respectively; while relative expression of GBSSI from the highest to the lowest were demonstrated by Hitam Melik, Hitam Pekat, SOJ A3, Janur Kuning, Berlian, Merah Bali, Blambangan A3, and Blambangan A2, respectively. Based on this research we conclude that Hitam Melik potentially produces higher tocotrienol and lower glycemic index than other studied varieties.
Interspecies Reconstructed Embryonic Cell Interaction between Campbell Hamster (Phodopus campbelli) and Mice (Mus musculus) Noer Muhammad Dliyaul Haq; Diah Pristihadi; Vista Budiariati; Dwi Budiono; Al Mukhlas Fikri; Mokhamad Fahrudin; Cece Sumantri; Arief Boediono
HAYATI Journal of Biosciences Vol. 28 No. 3 (2021): July 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.3.185

Abstract

Interspecies embryo transfer is a seldom-used method to increase the successful conservation of endangered species. The study aimed to determine the potential development of interspecies reconstructed embryos. The present study used two animal models, Campbell hamsters (Phodopus campbelli) and mice (Mus musculus). The isolated inner cell mass (ICM) of hamster embryos were injected into the mice embryos. The embryos were transferred to the pseudopregnant mice using non-surgery embryos transfer methods. The fetuses were collected at day 13.5 of gestation for morphometric measurement and cytochrome b (Cyt b) analysis which used to determine the species of obtained fetuses. The results showed that the viability, pregnancy rate, and embryonic implantation ability of the interpecies reconstructed embryos did not differ significantly (p>0.05) compared to non-reconstructed embryos. Morphometric measurement showed that the crown-rump (CR) and the weight of fetuses in the reconstructed group were significantly higher than non-reconstructed group (p<0.05). According to Cytb analysis, the species of obtained fetuses were mice, while the population of hamster cells were found only in the blighted ovum (resorption). Therefore, it can be concluded that interspecies reconstructed embryos are able to implant. However, the population of mice cells are only found to develop.
Detection of Mulatexin in Local Mulberry Plants (Morus spp.) from Bogor, West Java, Indonesia Yasinta Ratna Esti Wulandari; Yogiara Yogiara; Isabel Livena Young
HAYATI Journal of Biosciences Vol. 28 No. 3 (2021): July 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.3.193

Abstract

The mlx56 gene family is found exclusively in the mulberry (Morus spp.) plant, and its role is to defend against insect herbivores, yet it had never been discovered from Indonesia, which has been cultivating mulberry silkworm's (Bombyx mori) feed in order to produce silks. This research focused on studying the expression of mlx56 gene family in local mulberry plants from Bogor, West Java, using different plant organs which were leaf, stem, and root. Each sample was collected from four species of mulberry; Morus alba var. kanva-2, M. alba var. multicaulis, M. bombycis var. lembang, and M. cathayana. Genomic RNA samples were collected and synthesized into cDNA, amplification was done using eight primers of the mlx56 gene family and the products were sequenced. Sequences were then analyzed by BLAST. Results showed diverse mlx56 gene family expression among species of mulberry and plant organs. The mlx56-2, mlx56-4, and mlx56-7, were mostly expressed in all species and organs. The gene mlx56-3, which was not discovered in the previous study, was expressed in M. cathayana leaf and root also in the roots of other species. It was identified as chitinase-like protein LA along with mlx56-5, while other genes were identified as mlx56 based on BLAST.
Diversity and Abundance of Phytoplankton in the Coastal Waters of South Sulawesi Hasriani Ayu Lestari; M. Farid Samawi; Ahmad Faizal; Abigail Mary Moore; Jamaluddin Jompa
HAYATI Journal of Biosciences Vol. 28 No. 3 (2021): July 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.3.199

Abstract

Phytoplankton are primary producers that can be used as seawater condition indicators. Certain phytoplankton can proliferate, causing harmful algal blooms (HABs). The coastal waters of South Sulawesi, Indonesia are under pressure from land-based processes and activities resulting in inputs of organic and inorganic materials. This study analysed phytoplankton diversity and abundance in coastal waters around South Sulawesi. Phytoplankton were sampled and seawater parameters (salinity, temperature, turbidity, pH, nitrate concentration) measured in-situ at six stations around seven major river estuaries in three seaways (Makassar Strait, Flores Sea, Gulf of Bone). Phytoplankton taxonomic composition, abundance and indices of diversity (H’), evenness (E), and dominance (D) were analysed. Phytoplankton from 31 species and three classes (Bacillariophyceae, Cyanophyceae, Dinophyceae) were identified. Phytoplankton abundance and community structure differed significantly between sites and seaways but were not significantly correlated with water quality parameters although Dinophyceae abundance correlated significantly with observed pollution levels. Phytoplankton abundance was strongly influenced by the Dinophyceae, especially Ceratium furca, a potential HAB species; Cyanophyceae had the strongest influence on species richness but least on community structure. C. furca abundance was strongly correlated negatively with species richness, H’ and E, and positively with D, indicating negative impacts of this species on phytoplankton communities.
Antihyperglycemic Activity of Java Tea-Based Functional Drink-Loaded Chitosan Nanoparticle in Streptozotocin-Induced Diabetic Rats Monita Rekasih; Tjahja Muhandri; Mega Safithri; Christofora Hanny Wijaya
HAYATI Journal of Biosciences Vol. 28 No. 3 (2021): July 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.3.212

Abstract

Functional drinks containing the leaves of Java tea has been shown to offer many health functionalities, particularly to lower blood glucose levels, due to its bioactive compounds. Nanoencapsulation technology was reported being able to protect these bioactive compounds and also enhance their bioavailability. This study examined the use of nanoencapsulation techniques to improve the bioavailability of Java tea-based functional effervescent drink and enhance its antihyperglycemic activity. Three versions were prepared in this study: ready to drink (RTD) as the control, microencapsulated, and nanoencapsulated. They were all measured for their total phenolic content (TPC) before being tested for their antihyperglycemic activity. The TPC of RTD, microencapsulated, and nanoencapsulated Java tea-based functional drinks (JTFD) were 998,425, 735,433, and 663,517 ppm, respectively. The antihyperglycemic activities were evaluated by feeding each beverage to streptozotocin-induced diabetic Sprague Dawley rats for fourteen days. Nanoencapsulated JTFD was found to be more superior than the other two formulations to decrease the blood glucose level (7.98%), maintain the feed intake, body weight, improve the viability of Langerhans and beta-cells by 49.09%, 32.50%, respectively.
Antibacterial Activity of Flavonoids from Ethyl Acetate Extract of Milk Banana Peel (Musa x paradisiaca L.) Wiwik Susanah Rita; Ida Ayu Raka Astiti Asih; I Made Dira Swantara; Ni Luh Yuli Damayanti
HAYATI Journal of Biosciences Vol. 28 No. 3 (2021): July 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.3.223

Abstract

Infectious diseases caused by Staphylococcus aureus and Escherichia coli can be treated by milk banana peels (Musa × paradisiaca L.). This study aims to reveal the antibacterial activity of milk banana peel ethyl acetate extract against Staphylococcus aureus and Escherichia coli, and to identify their active compounds. Extraction was conducted by maceration at room temperature, followed by partition. Separation was carried out by column chromatography with mobile phase of chloroform: ethyl acetate (7.5:2.5) and stationary phase of silica gel 60. Antibacterial assay was performed by well diffusion method and identification of active compounds was analyzed by UV-Vis spectrophotometry and LC-MS/MS. Extraction of the peel powder produced methanol extract, partition of the methanol extract resulted n-hexane, ethyl acetate, n-butanol, and water fractions. Antibacterial activity assay shows that ethyl acetate extract was the most active to inhibit the growth of both bacteria. Separation with column chromatography resulted 5 fractions. Identification of the most active fraction with UV-Vis showed that the isolate gave maximum absorption at λ 339.00 nm (band I) and λ 262.00 nm (band II) which were thought to be flavonol (3-OH substituted). Analysis with LC-MS/MS shows that the most active fraction contained 3-methacrylate flavonols and 3-(furan- 2yl) acrylate flavonols.
Antibacterial and Toxicity Activities of Indonesian Herbal Medicine Extracts Used for Postpartum Treatment Wiwit Denny Fitriana; Sri Banun Titi Istiqomah; Devi Anggraini Putri; Taslim Ersam; Adi Setyo Purnomo Purnomo; Nurlatifah Nurlatifah; Sri Fatmawati
HAYATI Journal of Biosciences Vol. 28 No. 3 (2021): July 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.3.232

Abstract

Postpartum treatments have been used by local women in Indonesia for some time now. One commonly used postpartum treatment is the consumption of a traditional herbal medicine called PHM-1 during the first 40 days after childbirth. In addition, a second medicine known as PHM-2 is taken for days 41-80. However, sufficient scientific evidence about the benefits of this postpartum herbal medicine does not exist. In this study, the antibacterial abilities of PHM-1 and PHM-2 therefore was evaluated and showed positive potential for both. Low IC50 values were obtained against pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Rhodopseudomonas palustris, Ralstonia pickettii, and Staphylococcus epidermidis and compared with chloramphenicol as a positive control. The antibacterial activity of both PHM-1 and PHM-2 against R. pickettii had the highest inhibitory activity as evidenced by the lowest IC50 values of 11.16 and 6.26 μg/ml, respectively. Furthermore, an in vivo acute toxicity test showed that PHM-1 produced a low LD50 value of 3.28 g/kg BW for both of male and female mice. These results support the use of both PHM-1 and PHM-2 as antibacterial treatments for postpartum women.

Page 54 of 110 | Total Record : 1091

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