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Anakes: Jurnal Ilmiah Analis Kesehatan
Published by Universitas Mohammad Husni Thamrin
ISSN : 20885687     EISSN : 27456099     DOI : 10.37012/anakes
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology
Jurnal Ilmiah Analis Kesehatan merupakan jurnal yang berisi tentang artikel ilmiah dalam bidang ilmu analis kesehatan yang dilakukan oleh dosen maupun mahasiswa serta para peneliti.
Arjuna Subject : Profesi Kesehatan - Profesi Kesehatan (Lain-Lain)
Articles 320 Documents
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Efektivitas Hasil Fermentasi Limbah Kulit Durian (Durio zibethinus Murr) sebagai Reagen Alternatif dalam Pemeriksaan Protein Urine Nizar, Mardyana; Jihan, Selviana Ramadhan; Citra, Trisna; Eros, Tresnawati
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 2 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i2.3232

Abstract

The presence of proteinuria indicates glomerular permeability abnormalities.Urinary protein examination 6% acetic acid method is commonly used with commercial corrosive reagents, in its use must be cautious because it can cause burns, permanent damage to the eyes, and irritation of the mucous membranes. The use of acetic acid, such as vinegar, is not high-risk. The acid acetate, which can be used in urine protein testing, can be produced through a substance considered to be a waste, the durian skin. In durian skin contains carbohydrates with a huge 60-70% and still can be utilized. The aim of this study is to find out if there is any difference in the use of reagents resulting from the fermentation of durian leather waste with 6% commercial acetic acid as well as to know its effectiveness. Through liquidification, sacharization and aerobic, anaerobic fermentation processes assisted by the α-amilase enzyme, as well as the microorganisms Saccharomyces cerevisiae and Acetobacter aceti are able to produce acetic acid for urine protein testing by the method of acetate acid by heating. Reagents resulting from the fermentation of durian leather waste concentrations of 2.5%, 5%, 7.5% and 10% effective. Acetate acid levels measured by the method of titration of alkalimetry, the lowest rate at the concentration of 2.5% is 4.3% and the highest at the 10% concentration produces 5%. The research found that reagents derived from the fermentation of durian peel waste at concentrations of 2.5%, 5%, 7.5%, and 10% are effective for use as reagents in urine protein examination. Based on the statistical tests Kruskal Wallis came to the conclusion that there was no difference between the results of the test of urine protein using acetic acid results from fermenting durian skin waste with 6% commercial acetate.   Keywords: Acetic Acid, Durian Skin, Titration, Urine
Daya Hambat Ekstrak Bunga Kenanga (Cananga odorata) dan Rosella (Hibiscus sabdariffa L.) terhadap Staphylococcus aureus ATCC BAA - 1026, Pseudomonas aeruginosa ATCC 27853 dan Proteus mirabilis ATCC 2593 Zuraida, Zuraida; Nurdiani, Catu Umirestu; Rahayu, Cahyawati; Permana, Atna; Hariutami, Desi
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 2 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i2.2960

Abstract

Nosocomial infections or healthcare-associated infections (HAIs) are global health problems caused by pathogenic microorganisms, particularly antibiotic-resistant bacteria such as Staphylococcus aureus, Pseudomonas aeruginosa, and Proteus mirabilis. The increasing incidence of antibiotic resistance highlights the need to explore alternative antibacterial agents derived from natural products. This study aimed to evaluate the antibacterial activity of kenanga (Cananga odorata) and rosella (Hibiscus sabdariffa L.) flower extracts against Staphylococcus aureus ATCC BAA-1026, Pseudomonas aeruginosa ATCC 27853, and Proteus mirabilis ATCC 2593. The antibacterial activity was assessed in vitro using the disc diffusion method with various extract concentrations. The inhibitory activity of kenanga flower extract at a concentration of 100% showed average inhibition zone diameters of 27.50 mm against Staphylococcus aureus, 26.20 mm against Pseudomonas aeruginosa, and 24.20 mm against Proteus mirabilis. Meanwhile, rosella flower extract at a concentration of 100% produced average inhibition zone diameters of 33.10 mm against Staphylococcus aureus, 28.00 mm against Pseudomonas aeruginosa, and 25.20 mm against Proteus mirabilis. The results indicate that both kenanga and rosella flower extracts exhibit antibacterial activity against all tested bacteria, with inhibitory strength varying according to extract concentration. Rosella extract demonstrated higher inhibitory activity against Staphylococcus aureus, whereas kenanga extract showed significant antibacterial activity against specific test bacteria. In conclusion, kenanga and rosella flower extracts have potential as natural antibacterial alternatives to help control bacterial infections associated with nosocomial infections.   Keywords:  Antibacterial, Cananga odorata, Hibiscus sabdariffa L., Nosocomial infection, Antibiotic                                     resistance
Gambaran Kadar Elektrolit Darah Pada Pasien Diare Akut di Rumah Sakit Tk. II M. Ridwan Meuraksa Jakarta Kamaliyah, Saffanah Hayya; Rahayu, Cahyawati; Nurdiani, Catu Umirestu; Kristianingsih, Yuli; Nugroho, Heru Purwanto
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 2 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i2.3164

Abstract

Diarrhea is a major health problem in Indonesia with the risk of dehydration and electrolyte imbalance. Electrolytes play an important role in maintaining the balance of fluids, acids-bases, as well as nerve and muscle function. This study aims to determine the overview of serum electrolyte levels (Na⁺, K⁺, Cl⁻) in patients with acute diarrhea in kindergarten hospitals. II M. Ridwan Meuraksa. This descriptive study used secondary data from the medical records of 157 patients with acute diarrhea. The laboratory examination uses the Ion Selective Electrode (ISE) method. The results showed that hyponatremia occurred in 40.6% of adult patients and 37.7% of elderly patients. Hypokalemia was found in 39.5% of adult patients and 31.1% of elderly patients. Chloride disorders were also significant, with 32.2% hypochloremia and 25% hyperchloremia in adults, and 37.7% hypochloremia and 34.4% hyperchloremia in the elderly. In conclusion, most patients with acute diarrhea experience electrolyte imbalances, especially hyponatremia and hypochloremia. Regular electrolyte checks are necessary in the treatment of diarrhea to prevent complications such as cardiac arrhythmias and kidney dysfunction. Keywords: Acute Diarrhea, Blood Electrolytes, Sodium, Potassium, Chloride.
Hubungan Kadar Natrium, Kalium dan Klorida Pada Pasien Hipertensi Di Rumah Sakit Umum Daerah Koja Jakarta Utara Ellis Susanti; Atna Permana; Sumiati Bedah
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 2 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i2.2876

Abstract

Hypertension is a very common and quite high disease problem in the world. -The prevalence of hypertension in the population aged over 18years is still quite high at 30.8% in 2023 (Riskesdas 2023). One of the causes of hypertension is due to an increase in Sodium and Chloridelevels and a decrease in Potassium levels. This study aims to determine the description of Sodium, Potassium and Chloride levels in emergencyhypertension patients at Koja Regional General Hospital, North Jakarta. The research method used a cross-sectional with a sample of 100hypertension patients who underwent  sodium, Potassium  and  Chloride  examinations  at Koja  Regional General Hospital, East Jakarta. .The most abnormal Sodium results were in the early elderly age group 60-65 years (21%), Potassium (42%) and Chloride (30%) in the Pre- elderlyage group (46-59 years). The most abnormal Sodium results were in women (24%), Potassium in men (46%), while Chloride was obtainedequally between men and women (24%). Abnormal sodium (27%) and potassium (37%) results were most common in patients with stage I hypertension, while abnormal chloride results were most common in pre-hypertension patients (50%). It is recommended that patients with hypertension have their sodium, potassium, and chloride levels checked for monitoring and evaluation, as well as for monitoring the effectivenessof treatment.   Keywords: Chloride, Hypertension, Potassium, Sodium
Pengaruh Pemberian Virgin Coconut Oil Terhadap Kadar TNF- dan Jumlah Leukosit Pada Tikus Putih Jantan (Rattus norvegicus) Model Hiperkolesterolemia Mutmainnah; Ichwan Baihaki
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 2 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i2.3130

Abstract

  Hypercholesterolemia contributes to oxidative stress, which may cause dysregulation of the proinflammatory response as indicated by increased levels of TNF-α and leukocyte count, both of which are risk factors for metabolic syndrome. Exogenous antioxidants such as tocopherol are required to interrupt the chain reactions of free radicals, while lauric acid functions as an anti-inflammatory agent that enhances immunity. These compounds are contained in virgin coconut oil (VCO). Aimed to determine the effect of VCO administration on the reduction of total cholesterol levels, TNF-α concentrations, and leukocyte counts. This experimental study employed a post-test only control group design. Twenty-four male Wistar rats were randomly divided into four groups. Group K0 received neither a high-cholesterol diet nor VCO. Group K1 received a high-cholesterol diet without VCO. Groups P1 and P2 received a high-cholesterol diet along with VCO at doses of 0.9 mL/200 g BW/day and 0.45 mL/200 g BW/day, respectively. On day 21, blood samples were collected to measure total cholesterol, TNF-α, and leukocyte count at the PSPG UGM Laboratory. The One-way ANOVA showed significant differences in TNF-α and leukocyte counts among groups (p=0.000). Post hoc Tamhane’s and Tukey tests demonstrated that TNF-α and leukocyte levels in groups K0, P1, and P2 differed significantly from those in K1 (p<0.05). Administration of virgin coconut oil effectively reduced TNF-α, and leukocyte count levels.   Keywords: Leukocyte counts, TNF-α, VCO
Profiling Senyawa Metabolit Fraksi Etil Asetat Daun Moringa oleifera Menggunakan GC-MS dan LC-MS/MS Lenggo Geni; Ari Estuningtyas; Beti Ernawati Dewi
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 12 No. 1 (2026): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v12i1.3408

Abstract

ABSTRAK Latar belakang. Organisasi Kesehatan Dunia (WHO) merekomendasikan integrasi pengobatan herbal dalam program kesehatan nasional. Indonesia kaya akan tanaman obat, termasuk Moringa oleifera (M. oleifera) yang tumbuh subur di berbagai iklim. Fitokimia daun, biji, akar, dan bunganya dimanfaatkan secara tradisional untuk mengobati demam, antiinflamasi, sakit kepala, dan antimikroba. Namun, dasar mekanisme kerja fitokimia dari M. oleifera masih belum jelas, sehingga perlu dilakukan penelitian untuk mengeksplor senyawa-senyawa yang terkandung, khususnya pada daun M. oleifera. Penelitian ini bertujuan untuk mengidentifikasi fitokimia fraksi etil asetat ekstrak etanol daun M. oleifera menggunakan GC-MS dan LC-MS/MS. Bahan dan Metode: Fraksi etil asetat ekstrak etanol daun M. oleifera (Konimex, katalog MOT-10, 9 September 2021) dianalisis untuk senyawa aktif menggunakan GC-MS (Labkesda DKI Jakarta) dan LC-MS/MS (Puslabfor POLRI, Sentul, Bogor). GC-MS efektif untuk senyawa volatil, sedangkan LC-MS/MS cocok untuk senyawa non-volatil dan kompleks. Hasil. Analisis GC-MS pada fraksi etil asetat ekstrak etanol daun M. oleifera mengidentifikasi senyawa mayor yaitu asam linolenat (25,52%), metil linolenat (12,67%), asam palmitat (9,29%), dan linolenol (9,05%). Senyawa minor yaitu fitosterol (1,66%), oktilenol (1,48%), dan tetrahidroisoquinolin (1,07%). Analisis LC-MS/MS (database ChemSpider) menunjukkan senyawa mayor yaitu flavonoid (16,83%) yang terdiri dari kuersetin, kaempferol, dan glikosida, steroid (9,33%), dan asam karboksilat (8,37%), . Kesimpulan: Fraksi etil asetat ekstrak etanol daun M. oleifera mengandung senyawa potensial seperti flavonoid (kuersetin, kaempferol), steroid, dan alkaloid sebagai kandidat aktif terapeutik. Aktivitas biologisnya perlu dikonfirmasi melalui uji lebih lanjut, termasuk potensi antivirus, antioksidan, anti-inflamasi, dan antimikroba secara in vitro, in vivo, maupun in silico. Kata Kunci: Daun M. oleifera, Ekstrak etanol, Fraksi etil asetat.
Karakterisasi Profil Leukosit Diferensial pada Penderita Tuberkulosis Paru sebagai Data Dasar Profil Imunologis Pasien TB Regina Parahita Agustin; Prima Nanda Fauziah; Cahyawati Rahayu; Lenggo Geni; Imas Latifah; Ellis Susanti; Masdianto Masdianto; Atna Permana
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 12 No. 1 (2026): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v12i1.3656

Abstract

Pulmonary tuberculosis (TB) remains a major global health problem with high morbidity and mortality rates. Infection with Mycobacterium tuberculosis triggers immune responses that can alter leukocyte distribution, making differential leukocyte counts valuable in assessing patients’ immune status. This study aimed to describe the leukocyte differential count results in pulmonary TB patients at RSUD Pasar Rebo during 2023–2025, analyzed by gender and age. A quantitative secondary data analysis was conducted by grouping leukocyte parameters based on sex and age, then presented in frequency distribution and percentages. The results showed that most patients TB had normal basophil values (99.6%). Eosinophils were almost equally distributed between normal (44.4%) and elevated (44.4%). Neutrophils were predominantly normal (58.7%), while lymphocytes tended to decrease (52%). Monocytes were mostly within the normal range (56.5%), although elevated levels were also frequent (43.5%). By gender, males more often showed elevated eosinophils, decreased lymphocytes, and high monocytes, whereas females more frequently exhibited elevated neutrophils and normal monocytes. In adolescents, all parameters were normal except for monocytes, which were elevated; in adults, lymphocytes were mostly low; and in the elderly, increased eosinophils and decreased lymphocytes were predominant. In conclusion, pulmonary TB patients demonstrated varied leukocyte patterns, particularly decreased lymphocytes and increased eosinophils and monocytes, reflecting immune responses to Mycobacterium tuberculosis infection. Keywords: Differential Count, Leukocytes, Pulmonary Tuberculosis
Pengaruh Persepsi Penerapan Keselamatan Pasien, Kualitas Pelayanan, dan Implementasi Nilai-Nilai Syariah terhadap Persepsi Kepuasan Layanan Rumah Sakit pada Mahasiswa STIKes Widya Dharma Husada Tangerang Fitriyati Irviana
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 12 No. 1 (2026): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v12i1.3701

Abstract

This study analyzes the statistical associations of perceived patient-safety implementation, service quality, and implementation of Sharia values with perceived hospital-service satisfaction among students of STIKes Widya Dharma Husada Tangerang. The outcome construct was revised to clarify that respondents were students rather than patients; therefore, it is not interpreted as direct patient-reported satisfaction. A quantitative cross-sectional survey was conducted among 50 students selected purposively based on their knowledge of health services and patient safety. Data were collected using a five-point Likert questionnaire and analyzed using multiple linear regression. All questionnaire items met item-validity criteria, and construct reliability was high (Cronbach's alpha=0.879-0.932). The regression model was significant, F(3,46)=120.284, p<0.001, R²=0.887, adjusted R²=0.880. Perceived patient-safety implementation was not significantly associated with perceived service satisfaction (B=-0.201; β=-0.170; p=0.089), whereas service quality (B=0.367; β=0.589; p<0.001) and implementation of Sharia values (B=0.699; β=0.637; p<0.001) showed positive significant associations. Sharia-value implementation had the largest standardized beta. The Kolmogorov-Smirnov residual test yielded p=0.001, indicating that the normality assumption was not met; inferential findings therefore warrant cautious interpretation. The findings highlight the importance of service interactions and Sharia-oriented values in shaping students' evaluations of hospital-service satisfaction but should not be generalized as patient-reported satisfaction. Keywords:       Patient Safety, Service Quality, Sharia Values, Service Satisfaction, Health Students
Hematopoiesis Klonal sebagai Prediktor Risiko Transformasi Leukemia dan Potensinya sebagai Target Precision Medicine: Sebuah Tinjauan Literatur Dina Garniasih; Maria Agata Chaja; Prima Nanda Fauziah
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 12 No. 1 (2026): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v12i1.3820

Abstract

Clonal hematopoiesis (CH) is characterized by the expansion of hematopoietic cell clones carrying somatic genetic alterations in the absence of diagnostic criteria for a hematologic neoplasm. Although most individuals with CH do not progress to malignancy, certain molecular and clinical features may increase the risk of developing myeloid neoplasms, including acute myeloid leukemia (AML). Understanding the factors that influence clonal expansion and evolution is essential for improving risk stratification and advancing precision medicine approaches.This review aimed to analyze the association between the molecular and clinical characteristics of CH and the risk of progression to myeloid neoplasms, identify potential factors for risk stratification, and evaluate their implications for biomarker development and precision medicine. A literature review was conducted of scientific publications addressing CH, clonal hematopoiesis of indeterminate potential (CHIP), clonal cytopenia of undetermined significance (CCUS), somatic genetic mutations, clonal dynamics, progression to myeloid neoplasms, biomarkers, and precision medicine approaches. The analysis focused on mutation types and combinations, clone size and variant allele frequency (VAF), the dynamics of clonal expansion, the presence of cytopenia, clinical factors, and alterations in the hematopoietic microenvironment that may influence disease evolution. Available evidence indicates that the risk of CH progression is heterogeneous and cannot be determined solely by the presence of a single mutation. Mutations in DNMT3A, TET2, and ASXL1 are frequently detected, but their prognostic implications vary. The risk of progression tends to increase in individuals with larger clones, higher VAF or progressive clonal expansion over time, high-risk or multiple mutations, and concomitant cytopenia or features consistent with CCUS. Interactions among intrinsic alterations in hematopoietic cells, inflammation, and the hematopoietic microenvironment may also contribute to clonal selection and expansion. Integrating molecular profiles with clinical characteristics may provide more informative risk stratification than the use of hematological parameters alone. Nevertheless, evidence regarding interventions that directly target CH to prevent leukemic transformation remains limited. CH represents a preneoplastic clonal state that may progress to myeloid neoplasms in a subset of individuals. Mutation type, combinations of genetic alterations, clone size and dynamics, cytopenia, and microenvironmental factors should be considered when assessing the risk of progression. Integration of molecular and clinical data may support individualized monitoring and the development of precision medicine strategies. However, longitudinal and prospective studies are needed to validate biomarkers with consistent predictive value and to identify safe and effective interventions for preventing transformation to leukemia. Keywords: Acute Myeloid Leukemia, Clonal Hematopoiesis, Clonal Evolution, Somatic  Mutations,  Precision Medicine, Risk Stratification
Perbandingan Hasil Pemeriksaan Widal dan Tubex-TF Pada Pasien dengan Dugaan Demam Tifoid di RSUD Haji Makassar Suardi; Muh. Aswar; Nur Qadri Rasyid; Andi Fitri Azizah
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 12 No. 1 (2026): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v12i1.3840

Abstract

Typhoid fever is a systemic infectious disease caused by the bacteria Salmonella typhi and paratyphi A, B, and C, and remains a public health problem in developing countries, including Indonesia. The Widal test has limitations in the form of cross-reactivity and interpretation that is highly dependent on the cut-off titer and the local epidemiological context; therefore, a comparative testing method such as Tubex-TF is needed, although evidence regarding the comparison of results between the two methods remains limited. This study aimed to compare the results of the Widal and Tubex-TF tests in patients with suspected typhoid fever at the Haji Makassar Regional General Hospital. This was a descriptive observational study using a cross-sectional design involving 8 patients clinically diagnosed with typhoid fever by physicians, with serum or plasma samples obtained via accidental sampling. The Widal test was performed qualitatively and semi-quantitatively with a positive cutoff titer of ≥1/160, while the Tubex-TF test was conducted using the Inhibition Magnetic Binding Immunoassay method. Of the 8 samples, the Widal test yielded negative results in 2 samples (25%) and positive results in 6 samples (75%), while the Tubex-TF test yielded a borderline result in 1 sample, a weak positive in 4 samples, and a positive result in 3 samples. Borderline results were treated as negative; the proportion of positive results was 87.5% (7/8). The numerically higher proportion of positive Tubex-TF results compared to the Widal test in this preliminary study indicates the potential of Tubex-TF as a complementary test; however, further research with a clear gold standard and a larger sample size is needed before it can be concluded as an alternative diagnostic method. Keywords: Sensitivity, Widal, Tubex-TF, Typhoid Fever

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