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Anakes: Jurnal Ilmiah Analis Kesehatan
Published by Universitas Mohammad Husni Thamrin
ISSN : 20885687     EISSN : 27456099     DOI : 10.37012/anakes
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology
Jurnal Ilmiah Analis Kesehatan merupakan jurnal yang berisi tentang artikel ilmiah dalam bidang ilmu analis kesehatan yang dilakukan oleh dosen maupun mahasiswa serta para peneliti.
Arjuna Subject : Profesi Kesehatan - Profesi Kesehatan (Lain-Lain)
Articles 311 Documents
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Infeksi Oportunistik Hepatitis B dan Hepatitis C Pada Pasien Terinfeksi Human immunodeficiency Virus yang Menjalankan Terapi Antiretroviral Doni, Doni Setiawan; Handrianto, Indri; Nur Hidayatuloh, Arif; Nurmalasari, Ary; Farihatun, Atun
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 1 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i1.2217

Abstract

Human immunodeficiency virus is a virus that attacks lymphocytes which can reduce the human immune system, making it more susceptible to various opportunistic infectious diseases and can cause death. This can cause other diseases to infect the body, one of which causes cell inflammation and liver cell necrosis, known as Hepatitis B and Hepatitis C. The aim of this study is to determine the results of Hepatitis B Surface Antigen and Anti-Hepatitis C Virus examinations in patients positive for Human Immunodeficiency Virus. who underwent antiretroviral therapy at one of the Community Health Centers in Ciamis Regency. This research method is descriptive where the research was carried out on Human Immunodeficiency Virus positive patients at one of the Community Health Centers in Ciamis Regency with 35 respondents and the examination was carried out in the Community Health Center laboratory in May 2023. The results of this research obtained 2 (6%) HBsAg positive respondents and 1 (3%) respondent was positive for anti-HCV. In conclusion, HIV patients on antiretroviral therapy are at risk of developing Hepatitis B and Hepatitis C infections.  Key words          : anti-HCV, HBsAg, ELISA, Immunochromatography
Pengaruh Ekstrak Etanol Daun Sirih Merah (Piper crocatum) Terhadap Kadar Gula Darah Tikus Putih (Rattus norvegicus) Sapalma, Krisma Winditia; Ahmad, Tasrif
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 1 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i1.2219

Abstract

Red betel leaf is a medicinal plant that contains several active ingredients that are beneficial for health. One of the active ingredients in red betelleaves is flavonoids such as quercetin. Quercetin is a flavonoid compound which is found in many plants and has anti-diabetic properties to reduce blood sugar levels in the body. The aim of this research was to determine the effect of administering red betel leaf extract (Piper crocatum) on the blood sugar levels of rats (Rattus novergicus). This research was an analytical experimental research with pretest and posttest. The division of control and treatment groups used Simple Random Sampling sampling techniques. There were 4 groups, namely control, treatment 50 mg/kgBB, 100 mg/kgBB, and 200 mg/kgBB. Treatment was given for 21 days and then blood sugar levels were measured using a Clima MC 15 photometer. Research data was analyzed using the One Way ANOVA Test with SPSS. The results of the Shapiro-Wilk normality test were obtained with a value of sig>0.05; Levene Test homogeneity test with sig. value > 0.05; One Way ANOVA with a sig value <0.05. It can be concluded that there is a real effect on the blood levels of white rats (Rattus norvegicus) after administering ethanol extract of red betel leaves (Piper crocatum) at doses of 50 mg/kgBB, 100 mg/kgBB, and 200 mg/kgBB. kgBB. Key words: Diabetes mellitus, Ethanol Extract of Red Betel Leaves (Piper crocatum), White Rat (Rattus norvegicus)
Optimasi Metode Pemeriksaan HbA1c Secara Elektrokimia Berbasis Screen Printed Carbon Electrode Furqon, Ayi; Kania, Prina Puspa
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2314

Abstract

The quantification of HbA1c as an analyte used to monitor the success of diabetes treatment is generally examined in a laboratory with sophisticated technological equipment and professional staff. Point of care testing (POCT) is generally designed for practical examination at the patient's side. POCT HbA1c uses a screen-printed electrode (SPCE) reagent strip labeled with aptamer molecular imprinted polymer (MIP), boronate, or anti-HbA1c antibody and then quantified electrochemically. The linearity of the measurement works at a normal detection concentration of 5.5-8.5% but has not been tested at high critical concentrations. The purpose of this study was to optimize the electrochemical quantification method of HbA1c. The research procedure, SPCE was first activated with 0.1 M NaOH by cyclic voltammetry. Aptamer labeling (Fe3O4@MIPHbA1c) was carried out using the chronoamperometric technique and quantification was determined by the differential pulse voltammetry (DPV) technique. HbA1c stock of 6% and 10% in PBS pH 7.4 was used as normal and pathological concentration solution in accuracy and precision study (target quality CV <3%). The result, the average normal concentration of HbA1c was 5.9% ± 0.1%, CV=1.69% and pathological concentration was 10.4% ± 0.21%, CV=1.92%. The linearity test result of measurement ranged from 2%-15% R2=0.987. In conclusion, MIPHbA1c-activated SPCE showed optimal condition in HbA1c measurement in the normal and pathological range. Keywords: SPCE, HbA1c POCT, HbA1c Magnetit, HbA1c Aptamer
Skrining Talasemia Minor Pada Pendonor di Unit Donor Darah Palang Merah Indonesia Kabupaten Majalengka Winengsih, Wiwin; Novilla, Arina; Ningrum, Nining Ratna; Khairinisa, Gina
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2330

Abstract

Thalassemia is a hereditary hemolytic disease caused by a disorder in the synthesis of hemoglobin within red blood cells. Thalassemia prevention programs are beneficial for reducing or breaking the chain of thalassemia by preventing marriages between thalassemia carriers, which could result in thalassemia sufferers. One preventive measure is thalassemia screening. This study aims to identify the presence of thalassemia minor carriers among donors at the Indonesian Red Cross Blood Donation Unit in Majalengka Regency. The study is a descriptive quantitative research with a sample size of 92 donors who visited the Indonesian Red Cross Blood Donation Unit in Majalengka Regency. The thalassemia screening included hemoglobin tests, osmotic fragility tests, and erythrocyte indices. The results showed that all 92 donors had normal hemoglobin levels with an osmotic fragility test showing negative results using OTOF method. Therefore, further examination of the erythrocyte index was not conducted.. Based on these results, it can be concluded that there were no thalassemia minor carriers among the donors at the Indonesian Red Cross Blood Donation Unit in Majalengka Regency. Keywords  :Thalassemia, Donor,Osmotic Fragility, Hemoglobin
Gambaran Kadar Elektrolit Darah (Natrium, Kalium, Klorida) Pada Pasien Balita Usia 0-5 Tahun dengan Diare Akut Di RSAB Harapan Kita Jakarta Nurdiani, Catu Umirestu; Sari, Indah Novitha Fitria; Nugroho, Heru Purwanto
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2368

Abstract

Diarrhea is an increase in the frequency of defecation, which is characterized by a stool consistency that is thinner than usual. Viruses, bacteria, and parasites can cause diarrhea. Diarrhea is the second most common cause of death in toddlers. The main cause of death in toddler diarrhea is severe dehydration due to excessive fluid loss. Laboratory tests on diarrhea patients include blood electrolyte tests including sodium, potassium, and chloride. This quantitative research is conducted with secondary data obtained from medical records and then processed. The study was conducted on all 64 patients aged 0-5 years with acute diarrhea at RSAB Harapan Kita. The results showed that based on gender, it was dominated by males with the highest abnormal electrolyte levels in chloride at 34 people (53.13%) with the lowest chloride value of 81 mmol/L and the highest chloride value of 125 mmol/L. Based on age, most diarrhea patients were found to occur in toddlers (1-5 years) with the highest abnormal electrolyte levels in chloride at 29 people (45.31%) with the lowest chloride value of 81 mmol/L and the highest chloride value of 114 mmol. /L. The research that has been carried out can conclude that in patients suffering from diarrhea, the average value of the patient's sodium and potassium levels is normal, while the chloride levels are high. It is hoped that it is important for parents of diarrhea sufferers to always maintain a clean environment to prevent diarrhea and immediately go to the nearest doctor or hospital when the body shows symptoms such as fever and continuous defecation.   Keywords: Diarrhea, Sodium, Potassium, Chloride
Gambaran Indeks Eritrosit Pada Penderita Tuberkulosis Paru di Rumah Sakit Umum Mary Cileungsi Bogor Kristianingsih, Yuli; Rahayu, Cahyawati; Prakoso, Aryo; Pratiwi, Cindy Dui
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2370

Abstract

Tuberculosis is a chronic disorder that spreads to several organs. It is caused by bacteria (Mycobacterium tuberculosis), which primarily affects the lungs. Cases remain high in the community, making it a global health issue. Tuberculosis can cause hematological disorders, affecting hematopoietic progenitor cells and plasma components, particularly erythrocytes. The examination of erythrocyte indices in pulmonary tuberculosis patients aims to determine the type of anemia. The purpose of this study is to describe the erythrocyte index values in pulmonary tuberculosis patients at Mary Cileungsi General Hospital, Bogor. This study is quantitative research using secondary data collected from medical records and then processed. The study involved 80 tuberculosis patients. The results showed that 48 patients (60%) had normal MCV, 30 patients (37%) had low MCV, and 2 patients (3%) had high MCV. For MCH, 48 patients (60%) had normal values, 30 patients (37%) had low values, and 2 patients (3%) had high values. Regarding MCHC, 69 patients (86%) had normal values, 8 patients (10%) had low values, and 3 patients (4%) had high values. The study concluded that there was an increase in erythrocyte indices in tuberculosis patients, both male and female, with an increase across all age groups. It is recommended to take medication regularly to reduce the risk of transmission. Additionally, this information can be useful in conducting erythrocyte index examinations to detect and prevent early indications of anemia. Keywords : Anemia, Erythrocyte index values, Pulmonary tuberculosis patients
Neuroprotective Effect of Red Betel (Piper crocatum) Extract on Brain Monosodium Gluatame (MSG)-Induced Rat (Rattus norvegicus) Baihaki, Ichwan; Mutmainnah
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2373

Abstract

Indonesian people prefer to eat fast food which is generally rich in flavorings. The most common ingredient found in fast food is MSG (monosodium glutamate). MSG consumption that exceeds the limit will produce free glutamate which is excitotoxic in various organs, one of which is the neurological system. Red betel has a fairly high antioxidant content which can inhibit excitotoxicity by inhibiting the occurrence of free radicals. The aim of this research was to determine the neuroprotective effect of red betel extract (Piper crocatum) on the brains of rats (Rattus norvegicus) given monosodium glutamate (MSG). This research is a laboratory experimental study with a completely randomized design method using 5 treatment levels, namely negative control (NC), positive control (PC), treatment 1 (T1), treatment 2 (T2), treatment 3 (T3). The NC treatment level was given 2 mL of distilled water. The PC treatment level was given 7gr/KgBB MSG. Levels T1, T2, T3 were each given MSG 7gr/KgBW in the morning, then after 8 hours, level P1 was given 200mg/KgBW of red betel extract, P2 was given 400mg/KgBW of red betel extract, and P3 was given 600mg/KgBW of red betel extract. Examination of the number of neurons in the dentate gyrus of the hippocampus using the ImageJ application and examination of calcium ion levels using the RAPIDLab 348X tool. Data analysis used the One Way Anova test with the SPSS 24 program. The results showed that there was a significant difference (p<0.05) between PC and NC. The results of the calcium ion parameters showed that only the 600mg dose treatment group functioned as a protector in the occurrence of hypocalcemia, while the histopathology parameters showed that the 400mg and 600mg dose treatment groups functioned significantly as a neuroprotector.
Gambaran Kualitas Mikrobiologi Es Batu Kristal Dan Es Batu Balok Pada Minuman Di Pujasera Daerah Stasiun Kereta Api Duren Kalibata Rizka, Devriola Betty Gayu; Rahayu, Cahyawati; Kristianingsih, Yuli
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2374

Abstract

Waterborne diseases are infectious diseases that are directly caused by water. An example of a common disease is caused by the bacterium Escherichia coli. Diarrhea can be transmitted through drinking water contaminated with Escherichia coli. Water is one of the sources of life for needs and for consumption (drinking), one of the water used is in the form of ice cubes. Ice cubes have a variety of benefits, one of the benefits is as a complement that is served along with drinks, the addition of ice cubes in drinks is to cause a cold and fresh sensation. Many beverage traders use block ice cubes that are not for their designation. Ice cubes for consumption must be safe from coliform bacteria and Escherichia coli in accordance with the quality standards of PERMENKES NO.2 of 2023. The purpose of this study was to obtain a description of the number of coliform bacteria and Escherichia coli in crystal ice cubes and block ice cubes used by beverage sellers in the Pujasera area of Duren Kalibata Railway Station. This study uses an exploratory descriptive method. The population of this study were beverage sellers who used crystal ice cubes as many as 10 people and ice cubes as many as 8 people in the Duren Kalibata Railway Station Area Food Court with a sample of 8 people each and 8 crystal ice cubes and 8 crystal ice cubes. The results showed that all samples of crystal ice cubes and block ice cubes were positive for Coliform Bacteria, 60% of block ice cubes were positive for Escherichia coli, while 80% of crystal ice cubes were positive for Escherichia coli. This is due to the lack of knowledge, attitudes and actions of traders about the quality of ice cubes that must be maintained. It can be concluded that crystal ice cubes and block ice cubes sold at the Duren Kalibata Railway Station Area Food Court do not meet the requirements of Environmental Quality Standards. For traders and the public to pay attention to the hygienic quality of snacks or drinks that are sold and consumed.  Keywords           : Block ice cubes, Crystal ice cubes, Beverages, Microbiological Quality
Pengembangan Metode Identifikasi Kadar Ion Besi Sampel Urin Berbasis Separasi Magnetik Permana, Ellsie Viendra; Khristian, Erick; Oktaviani, Nidya; Muhammad Rizaldi Ridwan; Siti, Salma; Maharani, Devi
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2457

Abstract

Kelebihan zat besi (iron overload) dapat disebabkan oleh berbagai faktor, termasuk gangguan genetik seperti hemokromatosis herediter dan penyebab sekunder seperti transfusi darah berulang atau asupan besi yang berlebihan, yang sering terjadi pada pasien thalasemia. Penelitian ini bertujuan untuk mengembangkan metode baru dalam menentukan kadar ion besi dalam urin. Beberapa senyawa pembentuk presipitat ion besi yang diidentifikasi meliputi sulfit, peroksida, dan hidroksida, dengan hasil optimum diperoleh dari presipitasi menggunakan hidroksida berdasarkan parameter kestabilan dan kecepatan pengendapan dengan magnet. Sebelum analisis, sampel urin diperlakukan dengan penambahan magnesium untuk menghindari gangguan dari presipitasi ion fosfat. Hasil pengendapan optimum menggunakan hidroksida dicapai pada menit ketiga untuk ion besi dengan kadar 0,5 – 2,5 g/L. Nilai absorbansi turbiditas awal pada menit pertama berkisar antara 0,336 hingga 0,44, sedangkan absorbansi maksimum saat presipitasi maksimum berkisar antara 0,393 hingga 0,806, meningkat seiring dengan kenaikan konsentrasi Fe²⁺ dalam larutan. Setelah penarikan magnet dengan palladium ke dasar kuvet, absorbansi menurun dan stabil sejak menit kesembilan dengan rentang absorbansi 0,047 – 0,173. Absorbansi mencapai titik terendah pada menit keempat belas dengan rentang 0,002 – 0,03. Kurva standar yang dihasilkan dari selisih absorbansi turbiditas presipitat dan absorbansi supernatant setelah magnetisasi menunjukkan linearitas sebesar 0,94.
Analisis Kadar Karboksihemoglobin (COHb) dalam Darah Mahasiswa Perokok dan Bukan Perokok di Program Studi Teknologi Laboratorium Medis Universitas Mohammad Husni Thamrin Ramadres, Gerab; Masdianto, Masdianto; Kristianingsih, Yuli; Sugiantari, Nining
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 1 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i1.2475

Abstract

Smoking is a common behavior among adolescents. Consequently, it can affect the heart, central nervous system, and all oxygen-sensitive organs. This study aimed to determine differences in COHb levels in the blood of smokers and nonsmokers in the Medical Laboratory Technology Study Program at Universitas Mohammad Husni Thamrin. The Conway diffusion cell method, using sulfuric acid, was used to release carbon monoxide from hemoglobin molecules. UV-Vis spectrophotometry was used to identify single substances. COHb levels in 15 smokers were 0.53% and 0.26% in 15 nonsmokers. The study concluded that there were differences in COHb levels between smokers and nonsmokers. Both COHb levels were below the normal value of 3.5%, as determined by the Indonesian Minister of Health Regulation No. 70 of 2016. Keywords : Blood, COHb level, smoker  

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