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Jurnal Akuakultur Indonesia

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Published by Institut Pertanian Bogor

ISSN : 14125269 EISSN : 23546700 DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Jurnal Akuakultur Indonesia (JAI) merupakan salah satu sarana penyebarluasan informasi hasil-hasil penelitian serta kemajuan iptek dalam bidang akuakultur yang dikelola oleh Departemen Budidaya Perairan, FPIKâ€“IPB. Sejak tahun 2005 penerbitan jurnal dilakukan 2 kali per tahun setiap bulan Januari dan Juli. Jumlah naskah yang diterbitkan per tahun relatif konsisten yaitu 23â€“30 naskah per tahun atau minimal 200 halaman.

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Articles 569 Documents

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Effect of Thyroxine Hormone Administration in Female Broodstock on Metamorphosis and Survival Rates of Marble Goby (Oxyeleotris marmorata, BLKR.) Larva Hermawan, .; Junior, M. Zairin; Raswin, M.M.
Jurnal Akuakultur Indonesia Vol. 3 No. 3 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The experiment was conducted to study the effect of thyroxine hormone injection on female marble goby on metamorphosis and survival rate of their larva. Thyroxine hormone was injected into female at the dosage of 0,25 and 50 µg/g body weight. And then the female were reared with males in spawning tanks. Spawned eggs were hatched and the larva was observed for organogenesis, yolk absorption and growth in length. Formation of eye spot, swim bladder and body pigment becoming faster as the increase in thyroxine hormone dose. Yolk volume in larva that was obtained form thyroxine injected female decreased faster than those of control. Thyroxine hormone did not affected length growth and survival rate Key words: Marble goby, broodstock, thyroxine, metamorphosis, survival rate, larva

Feminization of Betta Fish (Betta splendens Regan) Through Embryo Immersion in Estradiol-17β Hormone Solution at the Dosages of 400 µg/I for 6, 12, 18 and 24 Hours Purwati, S.; Carman, Odang; Junior, M. Zairin
Jurnal Akuakultur Indonesia Vol. 3 No. 3 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The experiment was carried out to study the effect of embryo immersion in estradiol-17β hormone solution on sex ratio, hatching rate and survival rate of betta fish. Eyed embryo were immersed in 400 µg/I estradiol-17β for 0 (control), 6, 12, 18 and 24 hours, and then the larva were reared until reaching three month old. Immersion in estradiol-17β hormone increased percentage in female, and the highest value was achieved at 12 hours of immersion. Increasing treatment duration to 24 hours decreased the survival rate of betta fish. The lowest value of hatching rate was found at 24 hours of immersion. Key words: Feminization, betta fish, betta splendens, sex ratio, survival rate and hatching rate

Effect of LH-RHa Injection trough W/O/W LG (C-14) Emulsion on Gonad Development of Catfish (Pangasious hypophthalmus) Potalangi, N.; Toelihere, M.; Junior, M. Zairin; Supriyono, Eddy
Jurnal Akuakultur Indonesia Vol. 3 No. 3 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The objective of this research was to study the effect of LHRHa gonad maturity in broodstock of P. hypophthalmus through W/O/W LG (C-14) emulsion injection. The treatments consisted of control (A), 50 µg/kg fish wight (B), and 100 µg/kg fish weight (C), with five replications of each. Fish weight at the beginning of experiment was 2.0 kg/individual. The result showed that LHRHa in W/O/W emulsion had positive effect on egg maturation. This was shown by the value of average of eggs diameter. The maximum size of egg diameter for fish 701.52 ± 17.56 µm. The size of eggs was more homogenous in group B than those of group C and A. it was concluded that injection of LHRHa in W/O/W LG (C-14) emulsion if effective in promoting gonad maturation and oocyte development in the catfish

Pathogenicity Assay of Vibrio harveyi in Tiger Shrimp Larvae Employing Rifampicin-Resistant as A Molecular Marker Widanarni, .; Meha, D.; Nuryati, Sri; Sukenda, .; Suwanto, A.
Jurnal Akuakultur Indonesia Vol. 3 No. 3 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Rifampicin-resistant marker was employed as a reporter to assay pathogenicity of Vibrio harveyi in shrimp larvae. V. harveyi M. G3 and G7 that difference not schizotyping as shown by Pulsed-Filed Gel Electrophoresis (PFGE) used in this study. Spontaneous mutation was conducted to generate V. harveyi resistant to rifampicin. Two groups of shrimp post-larvae (PL5) were immersed for 30 min in 106 CFU/ml of mutants and wild type of V. harveyi, respectively; and then placed in a 2 liter shrimp rearing tank for five days. A control group was immersed in sterile seawater. Growth curve analysis and pathogenicity assay of V. harveyi showed that each of the V. harveyi mutant exhibited almost identical profiles to that of the wild type parental strain and did not show alteration in their pathogenicity. Sample from dead shrimp larvae showed that the dead shrimp larvae were infected by V. harveyi RfR, indicated that rifampicin-resistant marker effective as a reporter to assay pathogenicity of Vibrio harveyi in shrimp larvae. Key words: shrimp larvae, Vibrio harveyi, rifampicin-resistant, molecular marker

Control of Polychaetes by Dipping Infected Pearl Oyster on Different Salinity Hadiroseyani, Yani; Iswadi, .; Djokosetyanto, Daniel
Jurnal Akuakultur Indonesia Vol. 3 No. 3 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Dip treatment on pearl oysters (Pinctada maxima) was conducted in different concentrations of saline water to eliminate boring polychaetes. Results shows that polychaetes leave the oysters which treated on saline water at 0 ppt, 45 ppt, and 60 ppt as long as 15 minutes each. It also shows that the oysters got high survival rate 7 days after the treatment. Key words: Pearl oyster, Pinctada maxima, polychaetes, salt, dipping, survival rate

Intensity and Prevalence of Ectoparasites in Common Carp (Cyprinus carpio) fry from Traditional Pond and “Longyam” at Sukamulya Villages, Singaparna, Tasikmalaya Rustikawati, I.; Rostika, R.; Iriana, D.; Herlina, E.
Jurnal Akuakultur Indonesia Vol. 3 No. 3 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The experiment was done to find out species, intensity and prevalence of common crap fry from traditional ponds and "longyam" at Sukamulya village, Singaparna, Tasikmalaya. This experiment was conducted at Sukamulya village and Aquatic Biology Laboratory of Fisheries Department, Faculty of Agriculture, Padjajaran University form October 1st 2002 to October 31th 2002. Samples of common carp fry were obtained from six traditional ponds and six "longyam". The survey method was used in this experiment of common carp fry at difference type of pond were based on the Wilcoxon rank sum test. Intensity and prevalence of ectoparasites of common carp fry at traditional ponds and longyam was based on the Kruskal-Wallis test. The result of this experiment showed that eight genera of ectoparasites were found, consisted of three Platyhelminthes: Dactylogyrus (47,01%), Gryrodactylus (3,84%), Transversotrema (0,01%), four Protozoa: Ichtyophthrius (9,59%), Epistylis (1,05%), Trichodina (1,68%) and one Arthopoda: Acarus (0,01%), The intensity of Dactylogyrus was 28,83. Gryrodactylus 4.98, Transversotrema 0,17, Ichthyophthirius 13,51, Epistylis 4,68, Trichodina 25,14 Trichodinella 6,55 and Acarus 0,17. The prevalence of Dactylogyrus is 90,00% Gyrodactylus 44,82%, Transversotrema 0,37%, Ichthyophthirius 42,17, Epistylis 13,17%, Trichodina 82,59%, Trichodinella 17,04 and Acarus 0,37%. Key words: Intensity, prevalence, ectoparasites, common carp fry, traditional pond, "longyam"

Effect of Adding the White Legged Shrimp (Penaeus vannamei) on Growth and Survival of Tiger Shrimp (Penaeus monodon) in Intensive Farming Tarsim, .
Jurnal Akuakultur Indonesia Vol. 3 No. 3 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This experiment was carried out in the framework of a project to develop a viable shrimp polyculture technology under intensive farming. The objectives were to assess the effect of adding white legged shrimp (Penaeus vannamei) on growth and survival of tiger shrimp (Penaeus monodon) in intensive Farming. Tiger shrimp were stocked in 2800-3400 m2 earthen ponds at 40 individuals/m2 and reared for 133 days. White legged shrimp added at 70 day at 8±2 individuals/m2. Growth rate and total yield affected by addition of this species. Final affected by white legged shrimp addition. No adding effects were found on the water quality parameters. The results indicate that, at densities tested, white legged shrimp addition to the intensive farming of tiger shrimp polyculture is viable as species increase crop production Key words: Penaeus vannamei, P. monodon, growth, survival, polyculture

The potential of Trichoderma sp. as antibacterial and immunostimulant on white shrimp (Litopenaeus vannamei) Pebrianto, Catur A.; Sukenda, .; Widanarni, .
Jurnal Akuakultur Indonesia Vol. 9 No. 1 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The objective of this research was to study antibacterial and immunostimulatory effects of Trichoderma sp. extract on white shrimp, Litopenaeus vannamei. First experiment was conducted to evaluate inhibitory effect of Trichoderma sp. againts Vibrio harveyi, a pathogenic bacteria causing vibriosis disease on shrimp. Second experiment was conducted to evaluate immunostimulatory effect of Trichoderma sp. on shrimp immunity as well as protective effect against V. harveyi. A group of shrimp was injected with a minimum inhibitory concentration obtained at first experiment, and a week after, shrimps was challenged with V. harveyi (prophylactic). Another group was previously challenged with V. harveyi, and subsequently injected with Trichoderma sp. two fold of Minimum Inhibitory Concentration (MIC) a day after (therapeutic). Positive control, that was received only V. harveyi, and negative controls, that was received neither Trichoderma sp. nor V. harveyi were included in this experiment. Results of first experiment showed that a concentration of 600 ppm was a MIC of Trichoderma sp. to inhibit V. harveyi. While in the second experiment, the groups receiving Trichoderma sp., either prophylactic or therapeutic, showed protective effect against V. harveyi significantly higher than positive control and lower compared with negative control. Total haemocyte count (THC), differential haemocyte count (DHC), phagocytic index and phenoloxydase activity were different among the groups of prophylactic treatment or therapeutic treatment compared to control positive and negative treatments. In conclusion, Trichoderma sp. could be used in prophylactic and therapeutic treatments to combat infection of V. harveyi on L. vannamei. Key words: Trichoderma sp., V. harveyi, immunostimulant ABSTRAK Penelitian ini bertujuan untuk mempelajari pengaruh antibakterial dan immunostimulasi ekstrak Trichoderma sp. terhadap udang putih, Litopenaeus vannamei. Percobaan pertama dilakukan untuk mengevaluasi pengaruh penghambatan Trichoderma sp. terhadap Vibrio harveyi, bakteri patogen yang menyebabkan penyakit vibriosis pada udang. Percobaan kedua dilakukan untuk mengevaluasi pengaruh immonustimulasi Trichoderma sp. terhadap imunitas udang serta pengaruh protektif terhadap V. harveyi. Sekelompok udang disuntik dengan konsentrasi hambatan minimum (minimum inhibitory concentration, MIC) yang diperoleh dari hasil percobaan pertama, dan seminggu kemudian udang diuji tantang dengan V. harveyi (profilaksis). Kelompok udang lain diuji tantang dengan V. harveyi sebelumnya untuk kemudian disuntik dengan Trichoderma sp. (therapeutic). Kontrol positif, yang hanya diuji tantang dengan V. harveyi, dan kontrol negatif, yang tidak mendapat baik Trichoderma sp. maupun V. harveyi. Hasil percobaan pertama menunjukkan bahwa 600 ppm merupakan konsentrasi MIC Trichoderma sp. yang memberikan efek penghambatan maksimal terhadap V. harveyi. Sedangkan hasil percobaan kedua, kelompok udang yang mendapatkan Trichoderma sp. baik sebagai profilaksis maupun therapeutic menunjukkan bahwa Trichoderma sp. memberikan efek perlindungan terhadap infeksi V. harveyi secara signifikan lebih tinggi daripada kontrol positif dan lebih rendah daripada kontrol negatif. Total hemocyte count (THC) dan differential hemocyte (DHC), indeks fagositik dan aktivitas fenoloksidase kelompok perlakuan profilaksis atau perlakuan therapeutic berbeda baik dengan kontrol positif maupun negatif. Untuk itu dapat disimpulkan bahwa Trichoderma sp. dapat digunakan dalam tindakan pencegahan dan pengobatan infeksi V. harveyi pada udang putih. Kata kunci: Trichoderma sp., V. harveyi, immunostimulan

Hematology of common carp following DNA vaccination and koi herpesvirus challenge test Nuryati, Sri; Maswan, N.A.; Alimuddin, .; Sukenda, .; Sumantadinata, K.; Pasaribu, F.H.; Soejoedono, R.D.; Santika, A.
Jurnal Akuakultur Indonesia Vol. 9 No. 1 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The study was aimed to determine the effectiveness of DNA vaccine doses on hematological aspect which represent immune response and its influence on common carp survival rate. DNA vaccines encoding the viral glycoprotein of koi herpesvirus (KHV) have been proved to highly protect the fish under laboratory condition. A dose of 12.5 µg/100 µl vaccine had resulted in a survival rate of 96.67 % during 30 days after challenge test with a lethal dose of KHV. Fish vaccinated using lower doses, i.e. 2.5 and 7.5 µg/100µl showed 100% mortality after 15 and 19 days challenge test respectively, whereas non vaccinated fish as a control showed 100% mortality after 17 days challenge test. Total leucocytes of the vaccinated fish were higher than control until 42 days post vaccination, but declined afterward. Phagocytic index of the vaccinated fish using 12.5 µg/100 µl was declined after 49 days post vaccination or 7 days post challenge test. Key words: DNA vaccine, Koi herpesvirus (KHV), leucocyte, phagocytic index, Cyprinus carpio ABSTRAK Penelitian ini bertujuan untuk menguji pengaruh vaksinasi menggunakan vaksin DNA dengan dosis berbeda terhadap gambaran darah ikan sebagai respresentasi tanggap kebal ikan mas serta pengaruhnya terhadap tingkat kelangsungan hidup ikan mas. Vaksin DNA penyandi glikoprotein koi herpesvirus (KHV) dapat memberikan proteksi yang tinggi pada percobaan skala laboratorium. Vaksinasi dengan dosis 12,5 µg/100µl dapat mempertahankan kelangsungan hidup sebesar 96,67% selama satu bulan setelah uji tantang dengan virus KHV menggunakan dosis letal. Ikan yang divaksin dengan dosis yang lebih rendah yaitu 2,5 dan 7,5 µg/100µl mengalami kematian total berturut-turut setelah 15 dan 19 hari uji tantang, sedangkan ikan kontrol yang tidak divaksin mengalami kematian total setelah 17 hari uji tantang. Jumlah leukosit total ikan yang divaksinasi lebih tinggi dibanding dengan kontrol sampai hari ke-42, setelah itu mengalami penurunan. Indeks fagositosis ikan yang divaksin dengan dosis 12,5 µg/100µl mengalami penurunan setelah hari ke-49 atau 7 hari setelah uji tantang. Kata kunci: Vaksin DNA, Koi herpesvirus (KHV), leukosit, indeks fagositosis, Cyprinus carpio

Biocontrol agents in aquaculture: Production and their application Yuhana, Munti
Jurnal Akuakultur Indonesia Vol. 9 No. 1 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The use of biocontrol agents have become commonly accepted as a 'natural weapon' in aquaculture, either as an ecological bioremediator for low environmental quality, as well as the health promoter for cultured organisms. Biocontrol agents, which consist of beneficial microorganisms, are commonly applied as biosupplements in feeds. They are potential in replacing the use of antibiotic in inhibiting the pathogenic organisms. Therefore, their application has revealed the potential as an effective strategy to reduce the over use of antibiotics in controlling the pathogenic agents, avoid the spreading the drug resistance, or environmental deterioration of the negative effect by killing useful microorganisms. The development of suitable technology for microbial agents production, viability and stability, is a key area of research for industrial production. Production of biocontrol agents should be based on the microbial criteria, and the ability to withstand stress during processing and storage of products is crucial. This review makes an overview of biocontrol agents selection studies including techniques for isolation/identification, selection, production and its application in order to be accepted as a valuable product in aquaculture. Key words: Biocontrol agents, beneficial microorganisms, aquaculture. ABSTRAK Pemanfaatan agen biokontrol telah dapat diterima secara luas di dunia akuakultur sebagai senjata alami, baik sebagai bioremediator ekologis untuk memperbaiki kualitas lingkungan yang rendah maupun sebagai promoter/pendukung kesehatan untuk organisme-organisme yang dibudidayakan. Agen-agen biokontrol yang terdiri atas berbagai mikroorganisme yang menguntungkan umumnya diaplikasikan sebagai biosuplemen dalam pakan. Mikroorganisme tersebut potensial dalam menggantikan penggunaan antibiotik dalam proses penghambatan terhadap organisme patogenik. Oleh karena itu, aplikasinya yang sangat potensial dapat berguna sebagai strategi efektif untuk mereduksi penggunaan berlebih dari antibiotik dalam pengendalian agen patogenik, mencegah penyebaran sifat resistensi terhadap obat-obatan, atau mencegah kerusakan lingkungan dari kematian mikroorganisme yang bermanfaat. Pengembangan teknologi yang sesuai untuk produksi, viabilitas dan stabilitas dari agen biokontrol tersebut, merupakan kunci dari penelitian untuk produksi masal pada skala industri. Proses produksi dari agen biokontrol harus berdasar pada kriteria mikrobiologis. Sifat-sifat ketahanannya terhadap stress selama pemrosesan maupun penyimpanan produk adalah penting. Pada makalah ini disajikan teknik-teknik untuk isolasi, identifikasi, produksi dan aplikasi agen biokontrol untuk dapat diterima sebagai produk yang bernilai ekonomis dalam akuakultur. Kata kunci: Agen biokontrol, mikroorganisme yang menguntungkan, akuakultur.

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Apriana Vinasyiam

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