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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 103 Documents
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Search results for , issue "Vol 12, No 2 (2024): December" : 103 Documents clear
Distribusi Jenis Asing Invasif Melothria pendula L. (Cucurbitaceae) di Kawasan Konservasi Ilmiah Kebun Raya Bogor Cristina Mega Meyfiani Kekung; Iin Pertiwi A Husaini; Turhadi Turhadi
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12369

Abstract

Melothria pendula is belong to Cucurbitaceae family and originates from America continent. Previous research reported findings of M. pendula in several regions in Indonesia, such as in Bogor Botanical Gardens (KRB) as invasive species. The appearance of M. pendula in KRB has not yet been inventoried. As invasive species, M. pendula has the potential to be a threat to collection and native plants. This research aims to report the distribution of M. pendula in the scientific conservation area of KRB and measure the abiotic factors in the locations where M. pendula was found. This research was exploratory method which was carried out by exploring and morphological characterization of M. pendula. Measurement of abiotic factors, including temperature, air humidity, light intensity, and wind speed at each site where M. pendula was found. QGIS is used to mapping the distribution of M. pendula. Based on this study showed that there were 40 individuals of M. pendula which found distributed randomly in 15 sites (VG, VD, VB, XXIVA, IIM, IIC, IIP, XVI.VII, IVF, XXIVB, IIO, XVF, XXIIA, IIK, and seedling area) in KRB. M. pendula habitats in KRB characterized by forest land cover type with temperature of 31.1 ± 0.96 ℃, air humidity of 69 ± 2.73%, light intensity 3183.9 ± 3095 lux, and wind speed 0.7 ± 0.51 m/s.
The Correlation Between Age, Comorbidities, and Vaccination With The Mortality Rate of Covid-19 Patients Muin, Abdul; Menap, Menap
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12307

Abstract

The objective of this study is to determine the primary factors that contribute to the mortality rate of COVID-19 in Indonesia, with a specific focus on age, comorbidities, and vaccination status. A retrospective analysis was performed utilizing patient data obtained from the West Lombok Health Office in the year 2020. The findings indicated that individuals aged 59 years or older had a considerably higher risk of mortality, with an odds ratio of 0.065 (95% confidence interval: 0.014 - 0.305). Comorbidities, specifically hypertension and diabetes mellitus, were found to be substantial risk factors. The odds ratio for hypertension was 63.64 (95% CI: 1.232 - 30.608), while the odds ratio for diabetes mellitus was 2.00 (95% CI: 1.593 - 2.512). The study revealed that being vaccinated considerably decreased the likelihood of death, with an odds ratio of 0.071 (95% CI: 0.015 - 0.334). Nevertheless, engaging in self-isolation was found to greatly elevate the likelihood of mortality, as indicated by an odds ratio of 195.429 (95% confidence interval: 22.894 - 1668.196). The Chi-Square test revealed a statistically significant association between vaccination status and COVID-19 mortality, with a p-value of 0.000 < 0.05. The logistic regression analysis revealed that the type of isolation had a very significant and large effect on COVID-19 mortality. The odds ratio (Exp(B)) was 96.604, showing a strong association between the type of isolation and the mortality rate. The p-value was 0.000, further confirming the statistical significance of this relationship. Therefore, the type of isolation plays a critical role in determining the death rate of COVID-19 patients. These findings emphasize the significance of immunization programs and efficient isolation measures in decreasing COVID-19 mortality. In summary, this study offers significant information that can be used to develop more focused health policies in Indonesia. It emphasizes the crucial importance of vaccination and isolation measures in effectively managing the COVID-19 pandemic. Gaining insight into these factors can assist in customizing health treatments to mitigate risks and enhance clinical outcomes for COVID-19 patients in Indonesia
Hubungan Tingkat Pengetahuan Perawat Tentang Standar Asuhan Keperawatan dengan Kelengkapan Dokumentasi Asuhan Keperawatan di Puskesmas Korleko Hartini, Hartini; Menap, Menap; Taufandas, Maruli
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12339

Abstract

Nurses’ knowledge of nursing care standards is crucial to ensure the completeness of nursing care documentation. Complete and accurate documentation is an indicator of the quality of nursing services and patient safety. The aim of this study is to analyze the relationship between the level of nurses' knowledge about nursing care standards and the completeness of nursing care documentation at Puskesmas Korleko. This study uses an observational analytic design with a cross-sectional approach. The subjects of the study consisted of 33 nurses working at Puskesmas Korleko. Data collection was carried out through a questionnaire to measure the level of knowledge and a checklist to assess the completeness of documentation. Data analysis was conducted using the Spearman Rank correlation test to determine the relationship between variables. The results of the study showed that (1) 97% of nurses had good knowledge of nursing care standards, and 91% of them had very complete documentation; (2) a significant relationship between the level of nurses' knowledge and the completeness of documentation (correlation coefficient: -0.559, p-value: 0.001).
Evaluasi Kandungan Fitokimia, Kapasitas Antioksidan, dan Toksisitas Ekstrak Daun Stevia rebaudiana Frankson, Desvin; Hendrawan, Siufui; Ferdinal, Frans
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12151

Abstract

Oxidative stress leads to numerous clinical problems, such as autoimmune, vascular and respiratory impairment, as well as many other degenerative diseases. While daily exposure to UV light, air pollution, and smoke largely contribute to oxidative stress, endogenous antioxidants mostly fail to neutralize these high amounts of free radicals. Stevia rebaudiana or “candyleaf” is largely found in Indonesia, known for its application as a natural sweetener, owing to its content of stevioside compound. This study is experimental research which aims to assess the potential of stevia extract as an antioxidant supplement, which has not been widely applied. In vitro study was performed by extracting stevia leaves in methanol through simple maceration and percolation methods. Subsequently, the stevia extract was subjected to biochemical analysis, i.e. phytochemical screening, total phenolic content assay, antioxidant capacity assay, and toxicity assay. Herein, we found that stevia extract contains flavonoid, saponin, tannin, terpenoid, and phenolic compounds, with total phenolic content of 86.30±1.14 mg GAE/g DW. Moreover, antioxidant capacity assay through 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) method showed that IC50 of stevia extract is 20.13 µg/mL, slightly higher than Trolox (vitamin E analog) with IC50 of 16.50 µg/mL, which indicated that stevia extract is a very strong antioxidant. As for toxicity bioassay, Brine Shrimp Lethality Test indicated that stevia extract is non-toxic with LC50 value of 3388.44 µg/mL.
Teknik RAPD (Random Applied Polymorphic DNA) untuk Analisis Keanekaragaman Genetik pada Tanaman Ciplukan (Physalis angulata L.) di Kabupaten Labuhan Batu Utara Yusvita, Diana; Idami, Zahratul
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12889

Abstract

The ciplukan plant (Physalisangulata L.) can be used as traditional medicine, but so far people only know that this plant can only be used for the fruit. This research aims to determine the results of RAPD primary analysis which can produce polymorphic bands in Ciplukan Plants (Physalisangulata L.) in North Labuhan Batu Regency using different primers. This research is a quantitative research. Sampling was carried out in Bondar Village and Simpang Tiga Village, Kualuh Leidong District, North Labuhan Batu Regency. Then continued with molecular analysis at the Genetics Laboratory, Faculty of Science and Technology, North Sumatra State Islamic University, Medan. The results of RAPD (Random Applied Polymorphism DNA) analysis of ciplukan plants in North Labuhan Batu district using six different primers, namely the length of the nucleotides in the amplified DNA fragments, are shown in primers OPA-2 & OPA-3 are 600bp-3000bp, while OPA-5, OPA-7, OPD -11, & OPD-13 are 500bp-3000bp with different nucleotide sequences. The differences in the length and number of nucleotide sequences in ciplukan plants are caused by the plants used coming from different locations and several factors such as the environment, population size, natural conditions, reproductive methods and natural selection.
Isolation and Identification of Bacteria Producing Antibiotic Compounds from The Rhizosphere of Avicennia Marina Against Pathogenic Bacteria in The Mangrove Ecosystem Area of West Kalimantan Khairillah, Yuyun Nisaul; Alang, Hasria; Haryanto, Haryanto; Fitriagustiani, Fitriagustiani; Erwhani, Indri; Purnamawati, Ditha Astuti; Rahmawati, Annisa; Surtikanti, Surtikanti; Triwahyuni, Adelia
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12527

Abstract

Antibiotic resistance is a global problem in the health sector. The level of antibiotic resistance that occurs can be caused by several influencing factors, including 6 isolates of rhizosphere bacteria that were successfully isolated from the rhizosphere of the Avicenia marina plant. The purpose of this study was to isolate and determine the best minimum inhibitory concentration of rhizosphere bacteria of the A. marina plant and its effectiveness in inhibiting pathogenic bacteria S. aureus. This study is a quantitative research method using the isolation and characterization of rhizosphere bacteria, antagonistic test of rhizosphere bacteria and test of the results of the extraction of filtrate of rhizosphere bacteria culture of A. marina plants. The results of this study were (1) five of the isolates of bacteria that were successfully isolated were included in the Bacilus type and 1 isolate of bacteria included in the Cocobasil type; (2) there were 3 of the 6 isolates that could inhibit the growth of pathogenic bacteria S. aureus with an inhibition range of 10.04-4.42 mm; (3) Rhizosphere bacterial isolate (TN 1) is an isolate with the best inhibition diameter and is categorized as strong, namely 10.04 mm; (4) ethyl acetate extract of Rhizosphere bacterial isolate culture filtrate (TN 1), has a minimum inhibitory concentration of 0.50% against pathogenic bacteria S.aureus with an inhibition diameter of 7.18 mm which is better when compared to the positive control using chloramphenicol.
Analisis Beban Kerja dan Stres Kerja pada Perawat Muhajirin, Fahmi Abu Rizal; Sulaiman, Lalu; Setiwan, Sabar
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12970

Abstract

Perawat merupakan salah satu profesi di bidang kesehatan yang memiliki tanggung jawab besar dalam memberikan perawatan kepada pasien. Di Puskesmas, perawat seringkali menghadapi beban kerja yang tinggi akibat jumlah kunjungan pasien yang banyak serta tugas administratif tambahan. Beban kerja yang tinggi ini dapat mempengaruhi kesejahteraan psikologis perawat, menyebabkan stres kerja yang berpotensi menurunkan kualitas pelayanan kesehatan.  Penelitian ini bertujuan untuk menganalisis hubungan antara beban kerja dan stres kerja pada perawat di Puskesmas Tanjung, Kabupaten Lombok Utara. Studi ini merupakan penelitian kuantitatif dengan pendekatan survei. Sampel dalam penelitian ini sebanyak 41 perawat yang ditetapakan menggunakan teknik purposive sampling. Instrumen penelitian terdiri dari kuesioner beban kerja dan kuesioner stres kerja yang menggunakan skala Likert. Analisis data penelitian menggunakan uji kolerasi Pearson. Hasil analisis menunjukkan bahwa terdapat hubungan positif dan signifikan antara beban kerja dan stres kerja, dengan nilai koefisien korelasi (r) sebesar 0.65 dan p-value sebesar 0.000. Hal ini mengindikasikan bahwa peningkatan beban kerja berkorelasi dengan peningkatan tingkat stres yang dialami oleh perawat.
Potensi Bakteri Endofit dari Tanaman Jahe Sebagai Agens Pengandali Hayati Kristianingrum, Sabrina Ayu; Setiawan, Andree Wijaya; Jayanti, Ruth Meike
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12263

Abstract

The use of synthetic pesticides is not effective in reducing the prevalence of disease because it produces residues and does not support biodiversity. This is an unwise practice and can cause problems in the future.  The use of endophytic microorganisms is widely used to control plant diseases and pests, because it is known to have various pathogen inhibition mechanisms. Biological agents that are used to inhibit plant pathogens and have been widely developed, one of which is endophytic bacteria. The purpose of the research was to evaluate the potential of endophytic bacterial isolate from ginger plants as biological control agents. The type of research used is descriptive and exploratory. Sampling of healthy ginger plants was carried out at 5 points in two sub-districts, namely Getasan and Sumowono, Semarang Regency. Isolation is carried out by taking healthy ginger plants from the roots, rhizomes, fronds, and leaves. Characterization and selection of isolates were carried out by testing their phosphate dissolving ability, nitrogen anchoring, amylase and protease activities, and antagonist tests with Fusarium oxysporum in vitro. The results of this study are that (1) 20 pure isolates were successfully isolated consisting of 2 isolates derived from leaves, 4 isolates from fronds, 8 isolates from roots, and 6 isolates from rhizomes; (2) D91 isolate has the potential as a biological agent with an antagonist mechanism of 86.91% supported by protease enzyme activity, able to dissolve phosphate and anchor nitrogen.
Lama Fermentasi dan Konsentrasi Bunga Chamomile (Matricarica recutita) Terhadap Mutu Fisikokimia dan Organoleptik Teh Kombucha Fahilah, Khoiriyah; Hartati, Fadjar Kurnia; Yuniati, Yuyun
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.14182

Abstract

Kombucha is a health drink that has good benefits for the body, contains polyphenols and is antioxidant so it is said to be a functional drink. Chamomile positively contains flavonoid compounds, polyphenols, and tannins as antioxidants to ward off free radicals. The purpose of this study was to determine the effect of fermentation duration and concentration of chamomile flowers (Matricaria recutita) to produce kombucha drink with the best physicochemical and organoleptic quality. This study used a one-factorial Completely Randomized Design consisting of four treatments of a combination of fermentation duration (5 days and 7 days) and chamomile concentration (0.5% and 1%), each treatment was carried out three times. Parameters analyzed included color (L*a*b*), pH, antioxidant activity and organoleptic (taste, color, and aroma). The results showed that the length of fermentation and chamomile concentration had a significant effect on color (L*a*b*), pH, antioxidant activity and organoleptic parameters (taste and color). On organoleptic parameters (aroma) did not have a significant effect. The best treatment with antioxidant activity value of 67.09% in treatment F2C2 (7 days fermentation time and 1% chamomile concentration). The highest organoleptic quality was in the F1C1 treatment (5 days fermentation and 0,5% chamomile concentration).
Macrozoobenthos Diversity in The Fisheries Mangrove Ecotourism Area in Pulau Banyak Village Langkat District Manullang, Helentina Mariance; Lubis, Zulkarnain; Sabrina, Raja
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13660

Abstract

Mangroves are important ecosystems located in tidal areas and become habitats for various types of biota, including macrozobenthos. Macrozobenthos is a group of animals that inhabit the bottom waters of mangrove ecosystems. This study aims to analyze the diversity of macrozobenthos species in the mangrove ecotourism area of Pulau Banyak village, Langkat Regency, North Sumatra. This research was conducted from October 2022 to October 2023 in the fisheries-based mangrove ecotourism area in Pulau Bayak village, Langkat Regency. The research location was divided into three research stations. Macrozobentos observations were made once a month. Sample collection is done by collecting directly by hand, the samples collected are on the sample map (plot) measuring 1 x 1 meter making as many as 10 pieces for every 100 m2. Data were analyzed using the Paleontological Statistic (PAST) Version 4.0 tool. Parameters studied include diversity index, evenness, species richness, and dominance. Based on the results of the analysis, it is known that the diversity index is in the medium category with a value of 2.4-2.5, while the evenness index is in the high category with a value of 0.91 - 0.92, then the species richness index is in a low category with a value of 2.20 - 2.22 and the dominance index shows that there are no certain species that dominate this ecosystem with an index value close to 0. Thus, it can be concluded that this fisheries-based mangrove ecotourism area is in good condition and can support the lives of organisations associated with mangrove ecosystems.

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