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Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)
Published by Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medis Indonesia
ISSN : 27750108     EISSN : 27742504     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology
Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) merupakan jurnal AIPTLMI (Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia) yang dapat diakses secara terbuka (open acces) yang berfokus pada pengembangan ilmu pengetahuan Teknologi Laboratorium Medik (TLM) dengan tujuan untuk memperluas pengetahuan, pemikiran dan penelitian di bidang laboratorium kesehatan. Topik yang diangkat meliputi hematologi, kimia klinik, imunoserologi, imunohematologi, sitohitoteknologi, mikrobiologi, parasitologi, dan toksikologi klinik.
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 82 Documents
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Penentuan Taksiran Ketidakpastian (Uncertainty) dengan Metode Top-down dan Korelasinya Terhadap Total Error (TE) pada Parameter Hematologi Salvia Maharani, Farah; Ridwanna, Surya; Harianto, Harianto; Rinaldi, Sonny Feisal
Journal of Indonesian Medical Laboratory and Science Vol 5 No 1: April 2024
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v5i1.190

Abstract

Background: Uncertainty Measurement (UM) is defined as a parameter associated with a measurement result that characterizes a dispersion of values that can reasonably be associated with a quantity. ISO 15189 clause 5.5.1.4. states that the laboratory shall determine measurement uncertainty for each measurement procedure in the examination phase used to report measured quantity values on patients samples. Objectives: According to Westgard, there is debate about difference between the concepts of Uncertainty and Total Error (TE) so this study was conducted which aims to determine the uncertainty value of hematological parameters and determine the correlation of Uncertainty and TE. Materials and Methods: The material in this research are data of Internal Quality Control (IQC), External Quality Control (EQC), and Uncertainty data from the Sysmex XN-1000 calibrator. Results: Through the results the average uncertainty value of Sysmex XN-1000 2022 for leukocyte with low, medium and high levels, are ±19.05%, ±18.07 %, and ±15.94%, while for erythrocyte are ±4.46%, 4.10%, 4.16%, for hemoglobin are ±5.63, ±5.07, ±5.01, for hematocrit are ±8.99%, ±8.19%, ±8.19%, and for platelet are ±79.23%, ±62.23%, ±58.29%. Conclusions: Based on the uncertainty and TE calculated permonth for each lot number during 2022, the correlation was obtained between the Uncertainty and TE among leukocyte and hematocrit were stated to have a weak correlation, for hemoglobin and platelet it was stated quite correlated and erythrocytes are stated to be strongly correlated.
HUBUNGAN JUMLAH LEUKOSIT DENGAN JUMLAH TROMBOSIT PADA PASIEN DEMAM BERDARAH DENGUE (DBD) DI PUSKESMAS PIYUNGAN Devi, Affari Rahmawati Rima; Rohmah, Astika Nur; Astuti, Tri Dyah
Journal of Indonesian Medical Laboratory and Science Vol 5 No 1: April 2024
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v5i1.212

Abstract

Background: Dengue Hemorrhargic Fever (DHF) is an infectious disease caused by the dengue virus. Dengue fever is transmitted by Aedes aegypti and Aedes albopictus mosquitoes. In dengue fever sufferers, when a complete blood test is performed, the most common result is a decrease in the number of leukocytes and the number of platelets. Objectives: This study aims to determine the relationship between the number of leukocytes and platelets in DHF patients at Puskesmas (primary health center) Piyungan. Materials and Methods: The type of research used was analytical observational with a cross sectional research design and the data were collected using total sampling techniques. Data was obtained from medical records with a sample size of 26 patients. The relationship between the number of leukocytes and the number of platelets in dengue fever sufferers was tested using the Spearman’s correlation test.  Results: The result of analysis using the Spearman’s correlation test did not show a significant relationship between the number of leukocytes and the number of platelets in dengue fever patients (p = 0.0774), and the level of relationship was very weak with a positive relationship direction (r = 0.059). Conclusions: there was no significant relationship between the number of leukocytes and the number of platelets in dengue fever patients at Puskesmas Piyungan. A decrease in the number of leukocytes and platelets is a common condition that is often found in dengue fever patients.
AKTIVITAS ANTIBAKTERI EKSTRAK DAUN LIDAH MERTUA (Sansivieria tirfasciata var.laurentii) TERHADAP BAKTERI Klebsilla pneumonia Fitriani, Audiah; Sawitri, Nining Eka; Khatrina, Syahdapuan Dara; Nadifah, Fitri; Arisandi, Desto
Journal of Indonesian Medical Laboratory and Science Vol 5 No 2 (2024): In Progress
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v5i2.217

Abstract

Background: Pneumonia is an acute infection of the lower respiratory tract characterized by coughing and shortness of breath caused by Klebsiella pneumoniae bacteria. Sansevieria contains flavonoids, saponins, alkaloids, and steroids known to have antibacterial properties that can destroy the bacterial cell walls.  Objectives: This study aimed to determine the antibacterial activity of Sansevieria leaf extract against the growth of K. pneumoniae.  Materials and Methods: Detection of antibacterial activity of Sansevieria was conducted with disc-diffusion test. The presence of antibacterial activity is indicated by the appearance of inhibition (clear) zone after applying the extract at a concentration of 60-100% to the culture medium. As a positive control, the antibiotic chloramphenicol was used.  Results: The higher the extract concentration, the higher the inhibition zone formed. The inhibition zone formed from extract concentrations of 60%, 70%, 80% is classified as medium (d = 8; 8.5; 9 mm). Meanwhile, the inhibition zones at concentrations of 90% and 100% are 10.5 and 13.5 mm and are classified as strong. The clear zone of the positive control was 29 mm (very strong). Conclusions: Sansevieria leaf extract has the potential to inhibit the growth of K. pneumoniae bacteria.
Optimalization of NaOH concentration in alkaline lysis method on quality and quantity of Candida albicans DNA Putri, Nasya Desinta; Prayekti, Endah
Journal of Indonesian Medical Laboratory and Science Vol 5 No 2 (2024): In Progress
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v5i2.222

Abstract

Background: The concentration of NaOH in the alkaline lysis method needs to be optimized, especially when isolating DNA for its application in PCR which has not been maximized using the Candida albicans fungus which has a complex cell wall and is difficult to break. Thus, this optimization is expected to increase the efficiency of the alkaline lysis DNA isolation method in fungal species. Objective: The purpose of this studied was to determine the effect of optimizing the concentration of NaOH in the alkaline lysis method on the quality and quantity of Candida albicans DNA. Materials and Methods: This researched is experimental by performed DNA isolation used alkaline lysis method used NaOH concentrations of 1.5 N, 1.75 N, and 2.0 N and controlled (NaOH 0.2 N). Calculation of DNA quality and quantity using a nanodrop spectrophotometer. Results: The results showed the ordered of obtained the high to low DNA quality (A260/280) was NaOH concentration 2.0 N>1.75 N>1.5 N>0.2 N (1.86±0.44-0.95±0.18) while for DNA quantity was NaOH concentration 2.0 N>1.75 N>1.5 N>0.2 N (187.7±58.3-9.6±3.5 ng/?l). Conclusion: There is an effect of optimizing NaOH concentration on the lysis of fungal cell walls in the alkaline lysis method, namely increased the purity valued and concentration of sample DNA isolation results gradually. The use of 2.0 N NaOH concentration produced the best quality and quantity of DNA, namely 1.86±0.44 for DNA quality and 187.7±58.3 for DNA quantity.
Modification of CTAB incubation time in the isolation of DNA from Staphylococcus aureus Awaliyah, Arisma Putri; Prayekti, Endah
Journal of Indonesian Medical Laboratory and Science Vol 5 No 2 (2024): In Progress
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v5i2.224

Abstract

Background: DNA was an essential component in organisms that regulates biological activity. DNA analysis began with isolating pure, highly concentrated DNA, often used the CTAB method to isolate DNA from bacteria. DNA quality affected by incubation timed and temperature as well as its purity. The right incubation timed could optimize the degradation of proteins in the cell wall and maximize the released of DNA from the cell. DNA purity is good if the 260/280 absorbance ratio valued is 1.8-2.0. Objectives: This studied aimed to obtained the highest concentration and quality of DNA by modifying the incubation time of CTAB used Staphylococcus aureus DNA. Materials and Methods: This studied uses quantitative methods with the typed in experimental researched. The isolation stage included cell lysis with CTAB buffer and proteinase-K, purification stage with chloroform: isoamyl alcohol (24:1), DNA precipitation stage with isopropanol. Results: The average result of DNA purity obtained from the incubation treatment for 10 minutes was 1.89, for 20 minutes incubation was 1.46, and for 30 minutes incubation was 1.23. The average DNA concentration obtained from the incubation treatment for 10 minutes was 153 ng/µL, for 20 minutes incubation was 78.2 ng/µL, and for 30 minutes incubation was 26 ng/µL. Electrophoresis results at 10 minutes incubation time clearly visible DNA bands, 20 minutes incubation time DNA bands looked faint, and 30 minutes incubation time is not visible DNA bands. Conclusions: Based on the results of the studied, modification of CTAB incubation time with an incubation time of 10 minutes is the result of the highest concentration and quality of DNA using Staphylococcus aureus DNA.
Evaluation of critical value report of laboratory results based on national quality indicators at Bima general hospital Pratiwi, Rahma Indah; Pujiyanto, Pujiyanto
Journal of Indonesian Medical Laboratory and Science Vol 5 No 2 (2024): In Progress
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v5i2.235

Abstract

Background: To improve service quality and patient safety, Bima Regional General Hospital has set quality targets according to the national indicator. One such indicator is the national quality indicator for laboratory services, which aims to achieve a critical laboratory value report of 100%. Objectives: This research aims to evaluate the reporting of laboratory critical values by considering the achievement of predetermined quality indicator targets. Material and Methods: This research was carried out by collecting secondary data using the cross sectional design at Bima Hospital from January 2023 to March 2023 with a total sampling of critical value reports to the clinician or room nurses from all treatment rooms. Achievement of reporting time is calculated based on the percentage of timeliness of reporting critical value results compared to the total of all critical value reporting and is expressed as 'achieved' and 'not achieved'. Results: This research identifies the achievements of reporting that do not meet the target, and several obstacles and challenges that affect the efficiency and effectiveness of reporting. These findings indicate needs for improving coordination, communication and reporting procedures to support better achievement of quality indicator targets. Conclusions: The proposed improvement recommendations can help hospitals optimize the laboratory reporting process, thereby improving the quality of service and overall patient safety.
Analisis Unit Cost Quality Control Kimia Klinik dengan Metode Activity Based Costing di Laboratorium Rumah Sakit Paru dr. H. A. Rotinsulu Suryadi, Arfiah Azzahra Nissa; Kurniawan, Entuy; Rahayu, Ira Gustira; Rinaldi, Sonny Feisal; Hidayatullah, Taufik
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.192

Abstract

Hospital laboratories must comply with quality and safety standards as they are responsible for patients' health test results. Laboratory test results must be reliable, so the quality of laboratory services needs to be improved, including implementing quality control measures. Conducting a unit cost analysis for quality control activities is necessary to determine the expenses incurred for each quality control activity. The objective of this writing is to determine the unit cost for Clinical Chemistry Quality Control in the laboratory using the Activity-Based Costing method. Data collection techniques include interviews, observations, and document studies. Various costs are involved in determining the ABC method, including labor costs, depreciation costs for equipment or buildings, and other costs. In addition to cost data, cost drivers are also important in determining them. The equipment used in this research is the Sysmex BX with a total of 14 examination parameters, including glucose, total bilirubin, direct bilirubin, SGOT / AST, SGPT / ALT, total protein, albumin, urea, creatinine, uric acid, cholesterol, triglycerides, HDL, LDL. Based on the research results, the cost of QC for clinical chemistry examinations is Rp. 102,477
Optimalization of NaOH concentration in alkaline lysis method on quality and quantity of Candida albicans DNA Putri, Nasya Desinta; Prayekti , Endah
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.223

Abstract

Background: Candida albicans was a major caused of invasive candidiasis whose discovery by culture took a longed timed. Polymerase Chain Reaction (PCR) was a deoxyribonucleicacid (DNA)-based rapid diagnostic method. The simple DNA isolation method of alkaline lysis needed to optimized for Candida albicans, which had a complex cell wall that is difficult to broke. The concentration of NaOH in the alkaline lysis method is one of the things that affects the results of DNA isolation. Objective: The purpose of this studied was to determine the effect of optimizing the concentration of NaOH in the alkaline lysis method on the quality and quantity of Candida albicans DNA. Materials and Methods: This researched is experimental by performed DNA isolation used alkaline lysis method used NaOH concentrations of 1.5 N, 1.75 N, and 2.0 N and controlled (NaOH 0.2 N). Calculation of DNA quality and quantity using a nanodrop spectrophotometer. Results: The results showed the ordered of obtained the high to low DNA quality (A260/280) was NaOH concentration 2.0 N>1.75 N>1.5 N>0.2 N (1.86±0.44-0.95±0.18) while for DNA quantity was NaOH concentration 2.0 N>1.75 N>1.5 N>0.2 N (187.7±58.3-9.6±3.5 ng/ml). Conclusion: There is an effect of optimizing NaOH concentration on the lysis of fungal cell walls in the alkaline lysis method, namely increased the purity valued and concentration of sample DNA isolation results gradually. The use of 2.0 N NaOH concentration produced the best quality and quantity of DNA, namely 1.86±0.44 for DNA quality and 187.7±58.3 for DNA quantity.
The Relationship between C-Reactive Protein (CRP) Levels and HbA1c Levels in Type II Diabetes Mellitus Patients Utami, Rinda; Susanto, Zaenal Adi; Marsudi, La Ode; Maulida , Shofia; Mustofa , Khalid
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.234

Abstract

Background: Diabetes mellitus (DM) is a group of metabolic diseases characterized by hyperglycemia due to abnormalities in insulin secretion, insulin action, or both. Type II diabetes mellitus is caused by the pancreas gland being unable to meet the body's insulin needs. The HbA1c test reflects the average blood glucose level for 2-3 months or about 120 days before the test. C-reactive protein (CRP) is an inflammatory biomarker that is often used to assess the level of systemic inflammation. Elevated CRP levels have been associated with insulin resistance, endothelial dysfunction, and the development of atherosclerosis, all of which contribute to complications in diabetic patients. Therefore, further research is needed to evaluate the correlation between HbA1c and CRP levels, to better understand the relationship between glycemic control and systemic inflammation. Objective: This study aims to determine the relationship between C-reactive protein (CRP) levels and HbA1c levels in patients with Type II Diabetes Mellitus. Materials and Methods: This study used purposive sampling technique and analytic survey with cross-sectional approach. Results:  Data analysis using the Chi-Square test obtained a p-value of 0.536, where if Sig> 0.05 then it shows there is no significant relationship. So, there is no relationship between C-reactive protein (CRP) levels and HbA1c levels in Type II Diabetes Mellitus patients.
Effectiveness of Miana Leaves (Plectranthus scutellaroides) as a Natural Dye in the Identification of Soil Transmitted Helminth (STH) Eggs Samosir, Erica; Rahmah, Lestari
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.236

Abstract

Background: The use of natural dyes in microscopic examinations, such as the identification of Soil-Transmitted Helminth (STH) eggs, provides a safer and more environmentally friendly alternative. Miana Leaves (Plectranthus scutellaroides), which contain high levels of anthocyanins, have the potential to serve as an effective natural dye for staining STH eggs in fecal samples. Objectives: This study aims to evaluate the staining efficacy of miana leaf extract (Plectranthus scutellaroides) in identifying STH eggs by comparing different extract concentrations. Materials and Method: An experimental approach with descriptive data analysis was employed. Various concentrations of miana leaf extract (1:1, 1:2, and 1:3 ratios with distilled water) were tested on fecal samples positive for STH eggs. Results: The 1:2 concentration of miana leaf extract yielded optimal contrast and clear visualization of STH egg morphology, while the 1:1 concentration resulted in excessive staining and the 1:3 concentration produced pale staining. Conclusions: Miana leaf extract, particularly at a 1:2 concentration, is effective as a natural stain for STH egg identification. Further research should explore its application in broader parasitological diagnostic contexts.

Page 7 of 9 | Total Record : 82

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