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Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)
Published by Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medis Indonesia
ISSN : 27750108     EISSN : 27742504     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology
Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) merupakan jurnal AIPTLMI (Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia) yang dapat diakses secara terbuka (open acces) yang berfokus pada pengembangan ilmu pengetahuan Teknologi Laboratorium Medik (TLM) dengan tujuan untuk memperluas pengetahuan, pemikiran dan penelitian di bidang laboratorium kesehatan. Topik yang diangkat meliputi hematologi, kimia klinik, imunoserologi, imunohematologi, sitohitoteknologi, mikrobiologi, parasitologi, dan toksikologi klinik.
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 82 Documents
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Identification of Salmonella Sp. in Duck Eggs Sold in Larangan Village Putri, Nadia Auditia; Khusna , Umi Nihayatul; Jaman , Anwar
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.245

Abstract

Background: Duck eggs are one of the foodstuffs that are very susceptible to damage caused by microbes. The types of microbes that contaminate eggs are mainly from the Gram-negative bacteria type, including Salmonella. Objectives: The purpose of this study was to determine the salmonella sp bacteria in duck eggs sold in Larangan village Materials and Methods: The examination method used was observation of laboratory tests, isolation methods, and identification of bacteria using biochemical tests. The samples used were duck egg yolks, using Brain Heart Infusion Broth (BHIB) media, Salmonella Shigella Agar (SSA), gram staining, Triple Sugar Iron Agar (TSIA), Simon citrate, and Sulfide Indol Motility (SIM). Results: The results of the research that has been done on BHIB media obtained 6 samples showed bacterial growth, on SSA media 6 duck egg samples showed bacterial colony growth, on gram staining obtained gram-negative and rod-shaped bacteria in 6 samples, on TSIA media obtained positive results on 6 media, simon citrate test showed positive results on 6 media, SIM test on motile test 6 media obtained positive results, on sulfide test 5 media obtained positive results, while indole test 6 media obtained negative results. Conclusions: Based on the results of research on the identification of salmonella sp bacteria in duck eggs sold in Larangan village, Larangan sub-district, Brebes regency, Central Java, it was found that 6 duck egg samples contained salmonella sp bacteria.
Identifikasi bakteri Escherichia coli dan Salmonella sp. pada Spons Pencuci Piring Berdasarkan Lama Penggunaan Turrohmah, Alhidayah; Khusna, Umi Nihayatul; Jaman , Anwar
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.246

Abstract

Background: Sponges are tools used to clean cutlery. Dishwashing sponges are the main source of bacterial cross-contamination because they can transmit foodborne pathogens. Sponges that are used for a long time will have more potential to grow various microorganisms, such as bacteria from the Enterobacteriaceae family, including Escherichia coli and Salmonella sp. Objectives: The purpose of this study was to identify Escherichia coli, Salmonella sp..and the proper duration of use on sponges to avoid bacterial contamination. Materials and Methods: The type of research is descriptive, with a Quota sampling technique with a sample size of 6 dishwashing sponges at 6 points in the house (2 samples at one time), which have been determined on three indicators, namely 3,5,7 weeks of use. Observation and testing data collection methods. Results: The results showed negative Esherichia coli bacteria in all sponge samples with a duration of use of 3, 5, and 7 weeks, while positive Salmonella sp. bacteria in 1 sponge sample with a duration of use of 7 weeks. Conclusions: There were Salmonella sp. bacteria in 1 sample, namely a sponge used for 7 weeks and no Escherichia coli bacteria were found in all samples, all samples of dishwashing sponges with a usage period of 3, 5, and 7 weeks tested positive for bacterial contamination, so the minimum usage period for the sponge is 2 weeks, to avoid bacterial contamination from the dishwashing sponge.
Hubungan Personal Hygiene Dengan Kejadian Kecacingan Pada Anak Usia 6-9 Tahun Di Desa Pesarean Pagerbarang Tegal Jawa Tengah Khasanah, Isma Laelatul; Khusna , Umi Nihayatul; Jaman , Anwar
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.248

Abstract

Background: Ringworm disease is one of the diseases caused by infections with Soil Transmitted Helminths (STH) which usually attacks children because they are less able to maintain Personal Hygiene such as still liking to play in the soil,and not washing hands after playing.Objectives: : The aim of this research is to determine the relationship between personal hygiene and the incidence of worms in children aged 6-9 years in Pesarean Pagerbarang Village, Tegal, Central Java.Materials and Methods: This study uses an Observational Analytical research type using a cross-sectional approach , with a Incidental Sampling technique.Results: Based on the results of the questionnaire interview, it was found that 17 children (48.57%) had good personal hygiene, and 18 children (51.43%) had poor personal hygiene. Laboratory examination showed that 21 children (60.00%) were infected with STH, including Ascaris Lumbricoides, 18 children (85.71%). Tricuris Trichiura 1 child (4.76%), Necator Americaanus 2 children (9.52%). Conclusions: The results of analysis using chi-square obtained p-value = 0.000 for the relationship between personal hygiene and the incidence of worms and p-value = 0.013 for the relationship between nail cleanliness and the incidence of worms. This shows that there is a significant relationship between the two because ?<0.05. Keywords Incidence of worms; Personal hygiene..
MELANVEER : TEKNOLOGI PENDETEKSI PEMBULUH DARAH VENA UNTUK MENINGKATKAN PELAYANAN KESEHATAN) Adeline, Ivone; Maulana, Andri; Hartriningsih, Salzabila; Adi Prasetyanto, Wisnu
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.259

Abstract

Background: Intravenous (IV) procedures face challenges due to low nurse proficiency (50.8%) in Indonesian and difficulties in vein visualization, especially in patients with darker skin tones. Objectives: To develop an affordable, portable vein detection device, "Melanveer," capable of accurate visualization for all skin types. Materials and Methods: Melanveer uses near-infrared (NIR) light and Contrast Limited Adaptive Histogram Equalization (CLAHE) for real-time vein visualization. The prototype, made with 3D-printed components, was tested on 20 patients with varying skin tones (Very white to light white skin, Fair to light brown skin, and Dark to very dark brown skin). Results: Melanveer demonstrated 94% accuracy in vein detection, improved efficiency for medical staff, and enhanced patient comfort during IV procedures. Conclusions: Melanveer offers an innovative, cost-effective, and portable solution for accurate vein detection, addressing challenges in IV procedures and improving healthcare outcomes
Korelasi Kadar Troponin I dengan Serum Glutamic Oxaloacetic Transaminase (SGOT) dan Low Density Lipoprotein (LDL) Pada Penderita Penyakit Jantung Koroner Di RSUD Haji Provinsi Jawa Timur Amania, Salsabila; Handayati, Anik; Museyaroh
Journal of Indonesian Medical Laboratory and Science Vol 6 No 2: Oktober 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i2.279

Abstract

Background: Coronary Heart Disease (CHD) is a condition caused by atherosclerosis, which leads to reduced blood supply to the heart muscle. This condition occurs due to the buildup of plaques resulting form elevated levels of LDL.  Atherosclerosis can trigger myocardial infarction, leading to heart muscle damage and the release of biomarkers such as Troponin I and Serum Glutamic Oxaloacetic Transaminase (SGOT). Objectives: This Study aims to determine the correlation between Troponin I levels and SGOT as well as LDL levels in patients with CHD at Haji General Hospital in East Java Province. Materials and Methods: This study used an analytical observational study with a cross-sectional and purposive sampling. Results: The study results show that the majority of participants were aged 46–65 years (57%) and male (57%). Most patients had high Troponin I levels (70%), normal SGOT levels (63%), and high LDL levels (100%). The correlation analysis using the Spearman test showed a statistically significant correlation between Troponin I and SGOT levels with p value 0.046 and correlation coefficient r= 0.367, but the correlation between Troponin I and LDL levels was not statistically significant with p value 0.330 and correlation coefficient = 0.184. Conclusions:  The study found a correlation between Troponin I and SGOT in patients with CHD and no correlation between Troponin I and LDL in patients with CHD.
Inhibitory Effect of Nutmeg (Myristica fragrans) Leaf Extract on Biofilm Formation by Methicillin-Resistant Staphylococcus aureus (MRSA) Ramadhan, Fahri; Nugroho, Yusuf Eko; Faizal, Imam Agus
Journal of Indonesian Medical Laboratory and Science Vol 6 No 2: Oktober 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i2.294

Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is an opportunistic pathogen with a strong capacity for biofilm formation, which enhances resistance to antibiotics. Although nutmeg (Myristica fragrans) seeds and mace have been extensively studied, research on nutmeg leaves is limited despite their content of flavonoids, tannins, saponins, and triterpenoids with antimicrobial potential. Objective: This study evaluated the antibiofilm activity of nutmeg leaf extract against MRSA biofilm formation in vitro. Materials and Methods: Biofilm assays were conducted using MRSA isolates. The optimal incubation time for biofilm formation was first determined, followed by treatment with nutmeg leaf extract. Results: MRSA formed optimal biofilms at 48 h (OD = 0.101 ± 0.012). Nutmeg leaf extract significantly reduced biofilm formation (OD = 0.083 ± 0.010) compared with the negative control (OD = 0.118 ± 0.009) and the positive control, tetracycline (OD = 0.096 ± 0.011) (p = 0.001). While the reduction was statistically significant, the difference from tetracycline was modest. Conclusion: Nutmeg leaf extract demonstrated significant antibiofilm activity against MRSA in vitro. These findings support its potential as a complementary natural agent for managing biofilm-associated infections, warranting further studies to isolate active compounds and assess synergistic effects with standard antibiotics.
Evaluation of mangosteen peel extract in the kato-katz technique for enhanced visualization of helminth eggs Tanjung, Asbar; Maulidayanti, Sharfina; Br Situmorang, Ing Mayfa
Journal of Indonesian Medical Laboratory and Science Vol 6 No 2: Oktober 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i2.299

Abstract

Background: Mangosteen rind (Garcinia mangostana) contains anthocyanins, which have potential as natural dyes. One promising application is as a substitute for malachite green in the Kato-Katz technique, a fecal examination method used to detect helminth eggs such as Ascaris lumbricoides. Objectives: To evaluate the effectiveness of mangosteen peel extract at various concentrations (25%, 50%, 75%, and 100%) as an alternative staining agent in the Kato-Katz method. Materials and Methods: This laboratory-based experimental study utilized fecal samples positive for A. lumbricoides, which were examined using the Kato-Katz method with selophane tape soaked in mangosteen rind extract. Observed parameters included egg count per gram of feces (epg), clarity of egg morphology, and background contrast under the microscope. Results: The 75% concentration yielded the best performance, with optimal background staining, clear egg morphology, and the highest average egg count (853 eggs per gram). Concentrations of 25%, 50%, and 100% showed lower effectiveness. Conclusions: A 75% concentration of mangosteen rind extract is effective as a natural dye substitute for malachite green in the Kato-Katz method, providing good visual contrast and supporting accurate identification of helminth eggs
Correlation between Neutrophil Counts and Salmonella IgM in Typhoid Fever Patients at Emanuel Hospital Banjarnegara Haryanti, Anik; Nugroho, Yusuf Eko; Faizal, Imam Agus
Journal of Indonesian Medical Laboratory and Science Vol 6 No 2: Oktober 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i2.311

Abstract

Background: Typhoid fever is an infectious disease caused by Salmonella typhi and remains a significant public health problem in endemic regions. The incidence continues to rise annually, particularly among children. During infection, the immune system responds through both hematological and serological mechanisms, including increased neutrophil counts and the production of Immunoglobulin M (IgM). Neutrophils act as the first line of defense against bacterial invasion, while IgM is the earliest antibody produced during acute infection. Although many studies have independently examined hematological and serological parameters in bacterial infections, limited research has explored the relationship between neutrophil counts and Salmonella IgM in pediatric typhoid fever, especially in endemic areas such as Banjarnegara, Indonesia. Objectives: This study aims to analyze the relationship between neutrophil counts and Salmonella IgM results in pediatric patients with typhoid fever. Materials and Methods: This study is a cross-sectional design utilizing secondary data obtained from the Electronic Medical Records (EMR) and Laboratory Information System (LIS). Results: The results showed that 18 patients (47.4%) had normal or high neutrophil counts, while 2 patients (5.3%) had low neutrophil counts. Regarding Salmonella IgM results, 5 patients (13.2%) tested negative, whereas 33 patients (86.8%) tested positive. Conclusions: The Spearman statistical test yielded a p-value of 0.002 (p < 0.05) with a correlation coefficient of 0.480, indicating a significant relationship between neutrophil counts and Salmonella IgM results at Emanuel Hospital, Banjarnegara. The correlation between neutrophil counts and Salmonella IgM results is considered moderate.
Correlation between Hematocrit and Platelet Counts in Dengue Hemorrhagic Fever Patients: A Study at Emanuel Hospital, Banjarnegara Pancawati, Yulina; Pangesti, Ira; Nugroho, Yusuf Eko
Journal of Indonesian Medical Laboratory and Science Vol 6 No 2: Oktober 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i2.312

Abstract

Background: Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by the dengue virus and transmitted by Aedes aegypti mosquitoes. Without prompt management, DHF can lead to severe complications and death. The World Health Organization (WHO) identifies thrombocytopenia and increased hematocrit as key diagnostic parameters for DHF. Objectives: This study aimed to assess the correlation between hematocrit levels and platelet counts in patients with Dengue Hemorrhagic Fever at Emanuel Hospital, Banjarnegara. Materials and Methods: This cross-sectional study used secondary data from the hospital’s Electronic Medical Records (EMR) and Laboratory Information System (LIS). Statistical analysis was performed using Pearson’s correlation test. Results: The analysis showed a significant negative correlation between hematocrit and platelet counts in DHF patients (r = –0.760, p < 0.001). Conclusions: The findings indicate that an increase in hematocrit is associated with a decrease in platelet counts, emphasizing the importance of simultaneous monitoring of both parameters in the clinical management of DHF. This study contributes to local epidemiological data and underscores the potential of these laboratory indicators as practical tools for assessing disease severity.
Association Between HbA1c Level and Renal Function Markers in Patients with Diabetes Mellitus Muhammadiy, Noer Sidqi; Purniawan, Luki Herli; Afthoni, Muhammad Hilmi
Journal of Indonesian Medical Laboratory and Science Vol 6 No 2: Oktober 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i2.300

Abstract

Background: Diabetes mellitus (DM) is a major contributor to kidney failure worldwide. Glycated haemoglobin (HbA1c) is widely recognised as a marker for glycemic regulation, yet its association with renal function decline is not fully clarified. Objective: This study investigates the association between HbA1c levels and renal function indicators, namely creatinine, urea, and microalbumin, in individuals with DM. Materials and Methods: A cross-sectional approach was utilised, analysing retrospective data collected from the medical records of DM patients participating in the Prolanis program in Bondowoso Regency during 2024. Correlation tests and linear regression were used to determine the relationship between HbA1c and the selected renal parameters, with a statistical significance threshold of p < 0.05. Results: Most patients exhibited HbA1c levels and renal parameters within normal limits. Nonetheless, a statistically significant positive correlation was identified between elevated HbA1c levels and increased concentrations of creatinine (p = 0.020), urea (p = 0.01), and microalbumin (p = 0.01). Conclusion: Higher HbA1c levels are associated with deteriorating kidney function among DM patients. However, HbA1c should not be solely relied upon as a standalone marker for renal impairment. Further investigations are necessary to elucidate the biological pathways involved and to evaluate the potential of HbA1c control in mitigating kidney disease progression.

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