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Laury Marcia Ch. Huwae
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Jurusan Biologi - Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Pattimura Jl. Ir. M. Putuhena, Kampus Poka, Ambon, 97234, Maluku, Indonesia
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Biofaal Journal
Published by Universitas Pattimura
ISSN : -     EISSN : 27234959     DOI : https://doi.org/10.30598/biofaal.v1i1y2020
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Materials Science & Nanotechnology Medicine & Pharmacology
Biofaal Journal merupakan jurnal online yang menerima dan mempublikasikan tulisan-tulisan ilmiah dalam bentuk artikel hasil penelitian terbaru, short communication, maupun review terhadap artikel dari bidang keilmuan Biologi, Faal Tumbuhan, Faal Hewan, Faal Manusia dan Faal Olahraga. Biofaal Journal diterbitkan oleh Profesi Ahli Ilmu Faal, Jurusan Biologi FMIPA Universitas Pattimura. Dalam setahun Biofaal Journal memiliki 1 volume dan 2 Issue. Dewan editor, berasal dari rumpun ilmu biologi dan fisiologi yang aktif dalam publikasi ilmiah skala nasional dan internasional, sehingga mampu melakukan proses review secara profesional. Artikel yang diterima akan diterbitkan sesuai dengan waktu yang ditentukan yaitu pada bulan Juni dan Desember, dan akan tersedia gratis bagi pembaca
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 8 Documents
 1 
Search results for , issue "Vol 4 No 2 (2023): Biofaal Journal" : 8 Documents clear
TINJAUAN PUSTAKA: PEMANFAATAN TANAMAN BERKHASIAT ANTI AGING (ANTI PENUAAN) INDONESIA Sinurat, Elisabet Putriana; Diningrat, Diki Setya
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp058-071

Abstract

This study aims to determine the availability and quantity of literature regarding the use of antiaging plants obtained from the Google Scholar, Scopus, Pubmed and IPB Scientific Repository databases. The research design used is a literature review research design or literature review. Data collection techniques were carried out through literature searches on 4 different databases, namely: Google Scholar, Scopus, PubMed and IPB Scientific Repository. The results showed that there were 999 articles on the use of antiaging plants in the Google Scholar database, 200 articles on Scopus, 2,371 articles on PubMed, and 661 articles on the IPB Scientific Repository, bringing the total to 4,231 articles. After going through a complete process of screening and evaluating articles, 24 articles were obtained that met the search criteria. Based on the complete review process of the 24 articles, 24 types of plants were obtained that have the potential to be used as anti-aging, namely moringa, rosella flowers, shallots, purslane, green tea, nutmeg, arabica coffee, black soybeans, pagoda, patchouli, Dayak onions, potatoes, aloe vera, kaffir lime, yellow passion fruit, malaka, corn, raspberries, wungu, jernang rattan, pegagan, merbau, grapes, and pineapple.
UJI AKTIVITAS ANTIBAKTERI Vibrio sp. DENGAN EKSTRAK ETANOL DAUN SIRSAK (Annona muricata L.) Sianturi, Anggreni; Hitijahubessy, Hendro; Rumangun, Anastasia Putri Marshanda; Samid, Aprianti
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp072-080

Abstract

Study of the antibacterial ability of soursop leaf extract (Annona muricata L.) in inhibiting the growth of Vibrio sp. The maceration method with 70% ethanol solvent was used in this research to determine the yield, then the extract was diluted in stages from a concentration of 100% to 3.125%. The antibacterial test was done using the disc diffusion method in TCBS media and a phytochemical test was carried out on soursop leaves to analyze the secondary metabolite content. The extract yield obtained in this study was 3.77%. The test results showed that the largest zone of inhibition was found at 100% extract concentration of 11.45mm. The smallest inhibition zone was found in the 6.25% extract with an inhibition zone of 1.44mm, the diameter of the positive control (tetracycline) inhibition zone was 22.75mm, the diameter of 10% DMSO as a negative control was 0 mm. Phytochemical tests of soursop leaves show that they contain alkaloids, flavonoids, steroids, saponins and tannins.
EFEKTIFITAS ANTIBAKTERI Vibrio sp. DARI EKSTRAK DAUN MANGROVE Rhizopora apiculata Hitijahubessy, Hendro; Irmawati, Yuni
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp081-089

Abstract

Research was carried out by analyzing the antibacterial ability of the ethanol extractof mangrove Rhizopora apiculata leaves against Vibrio sp. Maceration using 70%ethanol resulted in an extract yield of 3.09%. The highest average inhibition zoneresults were found in mangrove leaf extract at a concentration of 100% with aninhibition zone of 24.68 mm. The smallest inhibition zone was found in mangrove leafextract with a concentration of 6.25% with an inhibition zone of 3.85 mm, a positivecontrol inhibition zone (30 mg tetracycline) of 26.35 mm and a negative controlinhibition zone (DMSO 10%) of 0 mm. This can be seen from the results of theaverage inhibition zone which shows strong antibacterial activity of mangrove leafextract at a concentration of 100% and moderate antibacterial activity of mangroveleaf extract at a concentration of 75% to 50% and no antibacterial activity atconcentrations of mangrove leaf extract below 25 %, the positive control inhibitionzone (30 mg tetracycline) was 26.35 mm and the negative control inhibition zone(DMSO 10%) was 0 mm. Phytochemical analysis was also carried out on mangroveleaves with the results showing the presence of several visible secondary metabolites.The secondary metabolites found in Rhizopora apiculata mangrove leaves consist ofalkaloids, flavanoids, steroids, saponins and tannins.
UJI PURI SEBAGAI GAMBARAN STATUS HIDRASI JANGKA PENDEK SELAMA JAM KULIAH PADA MAHASISWA Herawati, Elysabet; Wijayanti, Endah Tri
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp090-099

Abstract

The large number of activities without being balanced with sufficient consumption of drinking water can affect the hydration status of the body, namely the occurrence of dehydration. The study aims to describe the short-term hydration status of D-III Nursing Study Program students in terms of the PURI test results. The method used is the PURI test, namely the urine self-check method which is carried out in 2 tests. The research also used a survey method to support the results of the PURI test. Based on the results of the study, the short-term hydration status of D-III Nursing Study Program students based on the PURI test on the results of the 1st test showed that most of the students 15% experienced mild dehydration and 72% experienced severe dehydration. This is related to the habit of students who never bring drinking water to campus and most students do not consume enough water during the range of lecture hours until the lecture is over. The results of the 2nd PURI test showed that 76% of students had good hydration status because prior to testing students were encouraged to consume sufficient amounts of water. The results of the study show that students awareness of meeting body fluids to maintain body fluid homeostasis needs to be increased.
ANALISIS KEMAMPUAN DARI EKSTRAK ETANOL RUMPUT LAUT Ulva lactuca SEBAGAI ANTIBAKTERI Vibrio sp. DAN KAJIAN FITOKIMIANYA Metungun, Jakomina; Beruatjaan, Mariana Yerminal; Hitijahubessy, Hendro; Tamher, Endang
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp100-107

Abstract

An analysis of the antibacterial ability of Vibrio sp. has been carried out from the ethanol extract of Ulva lactuca seaweed and its phytochemical study. Analysis of the antibacterial ability of Vibrio sp. using the disk diffusion method was the main thing carried out in this research, then the extract yield was calculated from the maceration process of Ulva lactuca seaweed, as well as phytochemical analysis to support the antibacterial ability of Ulva lactuca seaweed against the bacteria Vibrio sp. The results of the extraction of Ulva lactuca seaweed with ethanol solvent obtained a yield of 3.67%. The results showed a moderate antibacterial activity of Vibrio sp. from Ulva lactuca seaweed extract at a concentration of 100% was 17.3 mm. Weak antibacterial activity was at a concentration of 75% of 12.525 mm and extracts below a concentration of 75% did not have inhibitory power against Vibrio sp bacteria. Phytochemical studies of Ulva lactuca seaweed found secondary metabolite compounds in the form of alkaloids, flavonoids, steroids, tannins and saponins. Based on the results of this research, it is hoped that the antibacterial ability of Vibrio sp. can be further investigated from ethanol extract of Ulva lactuca seaweed in vivo.
ANALISIS FILOGENETIK GEN gyrA DAN gyrB DARI GENUS MESORHIZOBIUM, RHIZOBIUM DAN ENSIFER Apituley, Edwin Thomas; Samson, Efraim; Killay, Amos
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp118-127

Abstract

Nucleotide sequence of gyrA and gyrB genes encoding subunit A and B of gyrase enzyme from genus of Rhizobium, Ensifer and Mesorhizobium were analyzed to determine evolutionary relationship among them. Maximum Likelihood and Kimura 2 parameter methods were used to construct phylogenetic tree and measure genetic distance. Phylogenetic tree based on nucleotide sequence of gyrA gene was compared to phylogenetic tree based on nucleotide sequence of gyrB gene. Comparison of identity percentage among nucleotide sequence of gyrA gene from genus Rhizobium, Ensifer and Mesorhizobium show similarity, where each of them have narrow range of identity percentage, however nucleotide sequence of gyrB gene from genus Rhizobium and Ensifer show wider identity percentage range than genus Mesorhizobium. Several member of genus Rhizobium and Ensifer have double copy of gyrB gene with identity percentage less than 50 percent between them. Topology of phylogenetic tree based on nucleotide sequence of gyrA gene have similar topology to topology of phylogenetic tree based on nucleotide sequence of gyrB gene, except for additional branch formed by one of additional copy of gyrB sequences. .
POTENSI KULIT BATANG Cinnamomum burmannii Bl. DALAM MENCEGAH INFERTILITAS; KAJIAN TERHADAP BERAT TESTIS DAN JUMLAH SPERMATOZOA MENCIT (Mus musculus) MODEL DIABETES MELLITUS TIPE-1 Moniharapon, Mechiavel; Ukratalo, Abdul M; Pattimura, Nadira; Samson, Efraim; Pangemanan, Victory O
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp108-117

Abstract

In individuals with diabetes mellitus, the increase in Reactive Oxygen Species (ROS) can damage the mitochondrial membrane, leading to the loss of potential mitochondrial membrane function in inducing apoptosis of sperm cells. Cinnamomum burmannii is a type of spice that contains antioxidants, thus capable of neutralizing free radicals and reducing oxidative stress caused by diabetes mellitus. Flavonoids also have an effect on increasing spermatogenesis. The aim of this research is to examine the role of methanol extract from Cinnamomum burmannii bark in increasing testis weight and spermatozoa count in type-1 diabetes mellitus mice (Mus musculus). The study employs a Completely Randomized Design (CRD) with five treatments and three replications. Twenty mice are divided into groups: K- (control), K+ mice injected with STZ without medication), P1 (dose of 200 mg/kg BW), P2 (dose of 400 mg/kg BW), and P3 (dose of 800 mg/kg BW). Before administering the Cinnamomum burmannii bark extract, blood sugar levels are measured in the K+, P1, P2, and P3 groups. If blood sugar levels have increased (>128 mg/dL), the P1, P2, and P3 groups are given the Cinnamomum burmannii bark extract according to the predetermined dosage. The treatment is carried out for 14 days. On the last day, surgery is performed to extract the testes and observe the spermatozoa count. The observation data on testis weight and spermatozoa count will be analyzed using ANOVA with SPSS 24.0. The results indicate that the administration of methanol extract from Cinnamomum burmannii bark significantly affects the increase in testis weight and spermatozoa count in type-1 diabetes mellitus mice. The increase in testis weight and spermatozoa count is proportional to the dosage given
INDUKSI POLIPLOID PADA KULTUR ANTERA Lilium longiflorum Thunb. Anggraeni, Anggraeni; Iriawati, Iriawati
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp128-136

Abstract

Poliploidisasi memiliki peranan penting dalam upaya pemuliaan beberapa tanaman hias, seperti pada bunga lili (Lilium sp.), dengan tujuan untuk menghasilkan bunga yang berukuran lebih besar dengan tangkai perbungaan yang lebih kokoh. Poliploid dapat diinduksi dengan senyawa colchicine pada waktu perendaman tertentu. Penelitian ini bertujuan untuk menentukan konsentrasi colchicine dan waktu perendaman yang optimum untuk menginduksi poliploidisasi. Eksplan yang digunakan berupa antera dari kuncup bunga L. longiflorum Thunb. yang berukuran 0,6-2,0 cm dan ditanam pada media inisiasi MS + 7,5 mM NAA + 0,75 mM BAP. Konsentrasi perlakuan colchicine yaitu 0, 100, 200, 400 ppm, dan perlakuan waktu perendaman yaitu 12, 24, 48, 72 jam. Hasil penelitian menunjukkan persentase pertumbuhan tunas dan akar terbaik pada perlakuan 100 ppm 12 jam dengan nilai persentase masing-masing (60,00 ± 7,30)% dan (100,00 ± 0,00)% dengan rata-rata partumbuhan tunas sebesar 1,40 ± 0,83 dan akar 5,13 ± 0,30. Konsentrasi colchicine optimum untuk menginduksi tanaman L. longiflorum poliploid adalah 200 ppm dan lamanya waktu perendaman 12 jam dengan menghasilkan tanaman tetraploid sebanyak 20%. Colchicine menyebabkan terbentuknya tanaman L. longiflorum tetraploid dengan menginduksi peningkatan jumlah kromosom (2n=4x=48), ukuran sel stomata, dan perubahan karakter fenotip.

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