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Life Science
Published by Universitas Negeri Semarang
ISSN : 22526277     EISSN : 25285009     DOI : https://doi.org/10.15294/lifesci
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Life Science publishes original and significant articles on all aspects of Life Sciences (Biology, Genetics, Biological Anthropology, Botany, Medical Sciences, Veterinary Sciences, Biochemical Genetics, Biometry, Clinical Genetics, Cytogenetics, Genetic Epidemiology, Genetic Testing, Evolution and Population Genetics, Immunogenetics and Molecular Genetics). The journal also covers ethical issues. It aims to serve as a forum for life scientists and health professionals.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 219 Documents
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Inovasi Pemeliharaan Masal (Mass Rearing) Corcyra cephalonica (Lepidoptera : Pyralidae) untuk Pakan Alternatif Burung Walet Aerodramus fuciphagus (Apodiformis : Apodidae) Damayanti, Nur; Ubaidillah, Rosichon; Priyono, Bambang; Rahayuningsih, Margareta
Life Science Vol 12 No 2 (2023): November 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i2.65240

Abstract

Swiftlets (Aerodramus fuciphagus) are small flying insectivorous birds and can make nests from their saliva. White swiftlets nests have very high economic value as food and are beneficial for health. Environmental factors are driving the emergence of feed innovation, namely mass rearing activities. The insects used in this study is the rice moth (Corcyra cephalonica). The purpose of this study is to analyze the innovation of mass rearing using bran, rice, and milled corn as feed media to influence the laying and life cycle of C. cephalonica. In this study using 3 feed media namely rice, bran, and milled corn using 4 treatments with different compositions 3 repetitions. The data analysis used in this study is a quantitative description. The data obtained were analyzed by statistical test Independent Sample t-Test. After being tested with statistically C. cephalonica egg production which had a significant difference, namely in treatments 2 (P2) with 3 (P3) and 4 (P4). The innovation of the feed media in treatment 2 had an effect on egg production, but the innovation of the three feed media had no effect on the life cycle of C. cephalonica, so the feed media that should be used as a feed innovation for Aerodramus fuciphagus is treatment 2, namely a combination of feed using corn (25%), bran (25%), and rice (50%) because they can produce more eggs than other treatments.
Analisis Korelasi Berbagai Faktor Budidaya terhadap Produktivitas Nira Siwalan (Borassus flabellifer L.) di Kecamatan Semanding Kabupaten Tuban Wahida, Afia Firna; Widiatningrum, Talitha; Kartijono, Nugroho Edi; Irsadi, Andin
Life Science Vol 13 No 1 (2024): May 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v13i1.65326

Abstract

Palmyra (Borassus flabellifer L.) is the identity plant of Tuban Regency. The main palmyra product in Tuban Regency is the sap. In the last 5 years (2015-2020) the productivity of sap has not increased. This study aimed to determine the correlation between cultivation factors and palmyra production in the Semanding Subdistrict, Tuban Regency, Indonesia. The research was correlative research with quantitative descriptive explanatory. The independent variables were several tree ownership, nurseries, fertilization, tapping methods, and control of plant pests. The dependent variable was the productivity of the palmyra sap. The research data sources were the people in the villages of Kowang, Gedongombo, Tegalagung, and Prunggahan, with a total sample taken of 103 people. Data collection was carried out using observation and semi-structured interviews. The results were then analyzed using the multiple linear regression application SPSS 25. The results showed that all the factors in the model, through the F test, had a significant effect simultaneously. Partially using the t-test, the factors that influence the productivity of the palmyra sap are pest control, the number of trees, and tapping methods. Therefore, the result confirmed the correlation between cultivation factors and palmyra production in the Semanding Subdistrict, Tuban Regency, Indonesia.
Pengaruh Penambahan Tepung Kulit Ari Kedelai terhadap Pertumbuhan Burung Puyuh (Cortunix-cortunix japonica) Isnaeni Karunia Annisa; Siti Harnina Bintari; Pramesti Dewi; Dewi Mustikaningtyas
Life Science Vol 12 No 2 (2023): November 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i2.66750

Abstract

Soybean hulls is a solid waste from the tempe industry. Utilization of soybean epidermis has not been done optimally. Soybean hulls contains isoflavone compounds and crude protein which are expected to be utilized in quail feed. This study aims to examine the effect of feeding with soybean hulls at different concentrations on the growth of quail. The research was conducted using a completely randomized design (CRD) with 4 treatments and 8 replications. The research material used 32 laying quails aged 42 days. Feed treatment with the addition of soybean epidermis consisted of P0 (without soybean hulls), P1 (7.5% addition of soybean hulls), P2 (10.5% addition of soybean hulls), and P3 (13.5% addition of soybean hulls). Parameters observed consisted of feed consumption, body weight gain, and feed conversion. The research data were analyzed using ANOVA, if it had a significant effect it was continued with the BNJ test (5%). The results of the analysis of variance showed that the addition of soybean hulls had a significant effect on feed consumption and body weight gain, but had no significant effect on feed conversion. It can be concluded that the addition of soybean husk as much as 7.5% is still effective in increasing weekly feed consumption and is able to provide the best feed conversion value.
Non-Ribosomal Inhibition of The Antibacterial Compound from Ethanol Extract of Gnetii gnemon Indra Lasmana Tarigan; Afidatul Muadifah; Madyawati Latief
Life Science Vol 12 No 2 (2023): November 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i2.67183

Abstract

Melinjo (Gnetii gnemonii Folium) is a plant species originating from Peninsular Malaysia and Indonesia, known that contain several bioactive compounds that can be utilized as antibacterial agent. The aims of this study to analyze the profile of the melinjo leaf (Gnetii gnemonii Folium) bioactive compound which has an antimicrobial function by inhibiting the growth of S. aureus and activity in Ribosomal expression. The profile analysis of bioactive compound of melinjo leaf was carried out by screening phytochemical study, HPLC and analysis of antibacterial activity was carried out using turbidimetry method against Bacillus cereus (Bc), Escherichia coli (Ec), and Staphylococcus aureus (Sa). The ethanol extract of melinjo leaves contains tannins, saponins, alkaloids, flavonoids, triterpenoids, with the highest levels being flavonoids 67,117ppm. Analysis of bacterial growth using OD600 showed relatively lower antibacterial activity compared to control. The identification of RNA expression showed that the antibacterial compound of melinjo leaf extract did not have inhibition activity through the ribosome pathway it was thought to have an antibacterial mechanism through non-ribosomal inhibition of bacteria.
Identifikasi Bakteri Asam Laktat Berdasarkan Kemiripan Fenotipik dari Kulit Nanas Varietas Queen di Kalimantan Barat yang Difermentasi secara Alami Medaando, Medaando; Rahmawati, Rahmawati; Turnip, Masnur
Life Science Vol 13 No 1 (2024): May 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v13i1.68553

Abstract

Queen pineapple, a variety of pineapple, is a fruit containing sugars that can be utilized as a source of nutrition by lactic acid bacteria. This study aims to identify the types of lactic acid bacteria from naturally fermented queen pineapple peels, identified based on phenotypic characteristics. Bacteria isolation was performed using the pour plate method with serial dilution up to 10^-6 using MRSA medium (de Man-Rogosa-Sharpe Agar). Characterization of lactic acid bacteria includes morphological, biochemical, and physiological characteristics. Bacterial identification refers to Bergey’s Manual of Determinative Bacteriology. The isolation and identification results of lactic acid bacteria obtained 6 isolates, namely, BAL1, BAL2, BAL3, BAL4, BAL5, and BAL6. Simple matching coefficient UPGMA analysis showed that BAL1, BAL2, and BAL4 had a phenotypic similarity of 83.8% to Lactobacillus fermentum; BAL3 and BAL5 had a phenotypic similarity of 90.9% to L. plantarum; and BAL6 had a phenotypic similarity of 87.9% to L. brevis. Jaccard coefficient UPGMA analysis indicated that BAL1, BAL2, and BAL4 grouped with L. fermentum with a phenotypic similarity value of 68.1%; BAL3 and BAL5 had a phenotypic similarity of 83% to L. plantarum, and BAL6 had a phenotypic similarity of 73.3% to L. brevis. This suggests that isolates BAL1, BAL2, and BAL4 are suspected members of L. fermentum species, BAL3 and BAL5 are suspected members of L. plantarum species, and BAL6 is suspected as a member of L. brevis species.
Viabilitas Oospora Peronospora manshurica Asal Kacang Kedelai (Glycine max) Impor Berdasarkan Perbedaan Suhu Inkubasi Muhammad Sunariya; Siti Khotimah; Ngaisatul Lutfiyah
Life Science Vol 12 No 2 (2023): November 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i2.69480

Abstract

Soybean (Glycine max) is the highest imported commodity entering West Kalimantan which has the potential to carry pathogens such as Peronospora manshurica. P. manshurica is a pathogen on soybean plants that causes downy mildew disease which can reduce the productivity of soybean plants. This fungus can spread with propagules in the form of oospores and mycelium on the seed coat like a grayish-white crust which can live if environmental conditions are suitable, such as temperature factors. The purpose of this study was to determine the viability of P. manshurica oospores tested with Triphenyl Tetrazolium Chloride based on differences in incubation temperature. This study used 240 symptomatic soybean seeds which were given three levels of incubation temperature treatment at 20oC, 30oC and 40oC with 8 repetitions using a completely randomized design. The oospore viability values were analyzed by one way ANOVA showing there were significant differences in each treatment. The viability values of live oospores obtained were 28.21% at 20oC, 17.06% at 30oC and 7.6% at 40oC. The highest viability value of live oospores was obtained at 20oC and showed that the higher the incubation temperature, the lower the viability value of P. manshurica oospores.
Kombinasi Pakan Limbah Kulit Pisang dan Kulit Ari Kedelai terhadap Bobot Larva Hermetia illucens (BSF) dan Indeks Pengurangan Sampah rida nur afifah; Dyah Rini Indriyanti; Priyantini Widiyaningrum; Ning Setiati
Life Science Vol 12 No 2 (2023): November 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i2.70837

Abstract

Hermetia illucens, often called BSF larvae, is an insect that has a high enough protein content so that it becomes an alternative animal feed that fulfills a high and quality protein source for animal feed. Bioconversion that utilizes organic materials by involving living organisms. Utilization of waste materials from banana peels and soybean husks as feed media for BSF larvae. This study aims to analyze the results of feeding a combination of banana peels and soybean epidermis on weight, waste reduction index and protein and fat content in BSF larvae. This study used an experimental method with the RAL technique (completely randomized design) with five treatments and four repetitions A : 100% banana peel waste, B : 75% banana peel + 25% soybean husk, C : 50% epidermis + 50% peel banana, D : 25% banana peel + 25% soybean epidermis, E : 100% soybean epidermis using 2kg of feed medium for each treatment. The results showed that the heaviest final weight was in treatment D (25% banana peel and 75% soybean epidermis) with an average final larval weight of 0.15 grams, the waste reduction index was 3.78%/day. The highest protein content in this study was in treatment A (100% banana peel) namely 48.97% and the highest fat content was in treatment D (25% banana peel and 75% soybean husk) namely 32.69%.
Identifikasi Keanekaragaman Famili Kenikir-Kenikiran (Asteraceae) di Kawasan Desa Kawak Pakis Aji Jepara Azzarkasyi, Anang Khoirunni'am; Widyaningrum, Fitri; Afsari, Hanisa Ayu; Ni'mah, Nazilatun; Jalil, Muhamad
Life Science Vol 13 No 1 (2024): May 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v13i1.71011

Abstract

Indonesia is one of the countries that has the highest level of biodiversity in the world. However, there are still many plants in Indonesia whose species have not been identified. This causes the need to identify and classify plants. This research aims to identify the diversity and dominance of plant species from the Asteraceae family in Kawak Village, Batealit District, Jepara Regency. This study was carried out on May 28, 2023 and June 1, 2023. The method used was the exploration method by determining sampling locations at 21 points from 3 hamlets in Kawak village. The tools and materials needed for this research include an accurate altimeter version 2.3.2, ziplock plastick, newsprint, cellphone camera, meter and internet access. There are 6 plant species from the Asteraceae family, including Wedelia trilobata, Crassocephalum crepidioides, Elephantopus scaber, Chromolaena odorata, Synedrella nodiflora, and Ageratum conyzoides. The plants obtained were collected using the herbarium method. The dominant plant species from the Asteraceae family in Kawak village is tapak Liman (Elephantopus scaber).
Aktivitas Imunostimulan Daun Elaeocarpus grandiflorus terhadap Jumlah Leukosit dan Histologi Limpa Tikus yang Diinduksi SDMD Nugrahaningsih WH; Eny Susanti; Mutiara Diah Nisa
Life Science Vol 12 No 2 (2023): November 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i2.73638

Abstract

The aim of this study was to examine the activity of various doses of E. grandiflorus leaf extract on the number of leukocytes, neutrophils, lymphocytes and histological features of the spleen in male rats induced by sheep red blood cells (SRBC). 25 rats were divided into 5 groups. The negative control was given 1% Na.CMC, the positive control was given an immunostimulant drug, and the three treatment groups received doses of 100mg/kg, 200mg/kg, and 400mg/kg. Oral treatment for 7 days. SRBC was injection on the 8th day. On the 13th day termination was carried out. The average leukocyte results were 6.54x103 cells/μL (K−), 6.22x103 cells/μL (K+), 10.8x103 cells/μL (P1), 10.98x103 cells/μL (P2), and 11.2x103 cells/μL (P3). The results of the number of lymphocytes have a percentage of 44% (KN), 50% (KP), 45% (P1), 52% (P2), and 50% (P3). The neutrophil count results were 44.3% (K−), 35% (K+), 37.4% (P1), 30.4% (P2), and 31.9% (P3). The results of the white pulp diameter measurements had an average of 418.0 µm (K-), 440.8 µm (K+), 471.2 µm (P1), 489.8 µm (P2), and 422.0 µm (P3). The averages obtained from germinal center measurements were as follows 252.1 (K-), 225.8 (K+), 212.8 (P1), 216.3 (P2), and 198.4 (P3). ANOVA showed no significant white pulp diameter difference (p > 0.05). In conclusion, the immunostimulatory activity test on rejasa extract on the parameters of leukocytes, neutrophils and lymphocytes had a normal average number. Meanwhile, administration of rejasa extract to the spleen histology have no significant immunostimulating activity.
Bioakumulasi Logam Besi (Fe) dan Seng (Zn) pada Ikan Bandeng (Chanos chanos) di Keramba Jaring Tancap Kelurahan Tanjung Mas, Semarang Arza, Alfira; Tri Martuti, Nana Kariada
Life Science Vol 12 No 2 (2023): November 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i2.73662

Abstract

Heavy metal contamination can cause a decrease in the quality of the aquatic environment which will have an impact on the quality of milkfish farmed in stick-net cages (KJT) by the local community. This Study aimed to analyze the Fe and Zn content in water and their bioaccumulation in Chanos chanos cultivated at KJT Tanjung Mas, Semarang. This research was conducted using explorative methods. The samples were taken by a random purposive sampling. Analysis of the content of heavy metals Fe and Zn in water samples and milkfish meat by atomic absorption spectrophotometry. The results of the three research stations showed that the concentration of Fe in water was 0.0270 – 0.0367 mg/L and Zn was in the range of 0.0050 – 0.0077 mg/L. The content of Fe and Zn in the waters of Tanjung Mas still in accordance with the quality standards of the Government Regulation of the Republic of Indonesia. The content of Fe contained in milkfish meat is 15.487 – 18.893 mg/kg, which exceeds the limit set by SNI (2009). The Zn content in milkfish meat is 11,504 – 12,568 mg/kg, which is below the limits of food security regulation in Ditjend POM. The conclusion of this study is that the heavy metal content of Fe and Zn in KJT Tanjung Mas waters and the heavy metal content of Zn in milkfish meat are below the threshold, while the heavy metal content of Fe in milkfish meat has exceeded the threshold.

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