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Stigma : Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa
Published by Universitas PGRI Adi Buana Surabaya
ISSN : 14121840     EISSN : 26219093     DOI : https://doi.org/10.36456/stigma.15.01
Core Subject : Education,
Mathematics
Jurnal STIGMA adalah jurnal ilmiah Biologi dan Biologi Terapan yang memuat artikel-artikel ilmiah. Jurnal STIGMA diterbitkan oleh Program Studi Biologi-Fakultas Matematika dan Ilmu Pengetahuan Alam - Universitas PGRI Adi Buana Surabaya. Terbit dua kali dalam satu tahun (April-Juli dan September-Desember), Jurnal ini memfasilitasi penelitian dengan tema Biologi Umum, Biologi Terapan, Bioteknologi, Aplikasi Ilmu Biologi.
Arjuna Subject : Umum - Umum
Articles 170 Documents
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Keanekaragaman Bakteri Di Sumber Mata Air Science Techno Park Desa Taloetan Kecamatan Nekamese Manansang, Tesalonika; Stanis, Stefanus; Mamulak, Yulita Iryani
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 17 No 01 (2024)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.17.01.8791.87-98

Abstract

Bacteria play an important role as bioindicators of water quality and also play a role in the ecological cycle that helps degrade organic materials in spring sources. The spring is one of the springs located in the development site of Science Techno Park Widya Mandira Catholic University located in Taloetan Village, Nekamese District, Kupang Regency. This study aims to determine what bacteria, dominant bacteria, and characters contained in the source of Science Techno Park springs in Taloetan Village, Sub-District. The stages in this study are as follows: 1. Isolation of bacteria from spring water sources 2. Bacterial purification and 3. Characterization of bacteria 4. Identification of bacteria is by matching the characters tested with Bergey's Manual of Determinative Bacteriology 9th. The results showed that sourced from two sampling points obtained as many as 20 bacterial isolates. at point one as many as nine isolates namely isolates and colon as many as eleven isolates namely BA 1.1, BA 1.2, BA 1.3, BA 1.3, BA 1.4, BA 1.7, BA 1.8, BA 1.9, BA 1.10, BA 2.1, BA 2.2, BA 2.3, BA 2.3, BA 2.4, BA 2.5, BA 2.6, BA 2.7, BA 2.8, BA 2.9, and BA 2.10. The conclusion of this study is that 20 bacterial isolates from samples of Science Techno Park springs in Taloetan village, nekamese have similarities with 13 genera, namely acidomonas, acinetobacter, microbacterium, bordetella, brucella, salmonella, xanthobacter, marinomonas, aeromonas, escherichia, spirillum, azotobacter, flavobacterium., the dominant genus in the springs of Science Techno Park in Taloetan Village, Nekamese District, namely the genus Acidomonas and the genus Salmonella, and the characteristics of bacteria in general are bacil, all bacterial isolates can ferment types of glucose sugar, all bacteria can’t grow at temperatures of 50ºC, cannot grow at pH 3, and cannot grow at NaCl 10% & 15%. Keywords: bacteria, spring source, isolation, characterization, and identification
Respon Eksplan Batang (Caulis) Planlet Tanaman Duku (Lansium domesticum Corr.) Terhadap Pemberian Kinetin Pada Media Murashige Skoog Melalui Kultur Jaringan YACHYA, ARIF; Nurokhman, Amin; Rasinta, Nanda
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 17 No 01 (2024)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.17.01.8854.105-111

Abstract

The duku plant (Lansium domesticum Corr) is a plant that is widely distributed in tropical regions such as Indonesia. One of the famous plants in Indonesia is duku Komering. Duku has a very sweet taste with proven production increasing every year. So it is necessary to cultivate duku using tissue culture techniques. The use of tissue culture techniques is able to produce seedlings in large quantities in a short time. This research aims to see the response of stem explants from plantlets. The research was carried out in the Tissue Culture room, Integrated Laboratory, Raden Fatah State Islamic University, Palembang for 49 days after induction (DAI). The type of research used is qualitative descriptive research and experimental methods with a completely randomized research design (CRD). This research used several varying concentrations of kinetin, including 0 ppm, 1 ppm, 2 ppm, 3 ppm and 4 ppm. on stem explants (caulis) from duku plantlets (Lansium domesticum Corr.). The results showed that there was no growth response to the administration of various concentrations of kinetin during the observation
PENGARUH PEMBERIAN KINETIN TERHADAP INDUKSI TUNAS PADA EKSPLAN STEVIA (Stevia rebaudiana B.) MELALUI KULTUR JARINGAN YACHYA, ARIF; Nurokhman, Amin; As’ad, Zuhdi
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 17 No 01 (2024)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.17.01.8855.99-104

Abstract

The stevia plant (Stevia rebaudiana B.) is found in the United States, and is often used as a mixture in drinks. In Indonesia, cultivation of stevia began in 1977 in West Java and Central Java. The natural sweetener content with low calories makes stevia a solution for diabetes sufferers. Conventional cultivation takes a long time and depends on soil conditions, climate and variety. Tissue culture is used for plant propagation in a short time, free of pests and diseases, and does not depend on the season. Growth Regulators (ZPT) such as kinetin are used to accelerate shoot growth. The tissue culture media used is Murashige Skoog (MS). This study aims to examine the effect of kinetin on shoot induction in stevia explants through tissue culture, with explants in the form of leaf petioles. This study used a Completely Randomized Design (CRD) with kinetin concentrations of 1 ppm, 2 ppm, 3 ppm and 4 ppm. The results of the research showed that all kinetin treatments on leaf petiole mother explants were able to form shoots with an average percentage of 100%. But it didn't show any influence.
IDENTIFIKASI DAN PEMANFAATAN TANAMAN OBAT DI KECAMATAN TALANG KELAPA KOTA PALEMBANG Hiras Habisukan, Ummi; Suhertini, Rizky
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 17 No 01 (2024)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.17.01.8890.112-131

Abstract

Medicinal plants are plants that can be used for curing the diseases because of its compounds. Identification of medicinal plants is needed to consider the kinds ofmedicinal plants. This study aims to know the species used as medicine and to process it for curing disease. This study is qualitative descriptive. The sampling technique is quota sampling with triangulation and data were analized by Miles and Huberman model. The results showed that 38 species from 22 family of medicinal plants aere found. Parts that are widely used were leaves, then roots, stems, flowers, flower petals, fruit, rhizomes, sap, tubers and all parts. Based on this study it can be seen that these plants can treat various diseases, including diabetes, hypertension, cholesterol, fever, diarrhea, skin itching caused by fungi such as phlegm and ringworm, vaginal discharge and nosebleeds, thrush, increase and accelerate breast milk production, accelerates the healing process of wounds and relieves menstrual pain. The most popular management method was boiled. Another method was mashed and then rubbed, dripped, dried and then affixed, brewed and consumed directlyas vegetables, vegetables or other food mixes.
Respon Pertumbuhan Tanaman Anggrek Bulan (Phalaenopsis amabilis) Terhadap Perlakuan Media Tanam dan POC Kulit Bawang Merah (Allium cepa) Firdaus El Salama, Shaila; Ngadiani; Andriani, Vivin
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 17 No 01 (2024)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.17.01.9173.132-139

Abstract

Moon orchid (Phalaenopsis amabilis) is one of the most fascinating orchid genus. The variety of colors, shapes and textures, as well as the aroma make moon orchids one of the most beautiful flowers. Ferns contain elements of Nitrogen (N), Carbon (C), Hydrogen (H), and silica. Coconut shells contain carbohydrates (K), phosphorus (P), sodium (Na) and magnesium (Mn). Shallots (Allium cepa) contain the elements Potassium (K), Calcium (Ca), Nitrogen (N), Phosphorus (P), Iron (Fe), Manganese (Mg), Magnesium (Mn), and Zinc (Zn). Meanwhile, non-nutritive chemical compounds include auxin and gibberellin hormones. This study used the complete randomized design (CRD) method, which consisted of 18 treatments and 3 replications. The growth parameters observed were number of leaves, leaf area, root length, plant weight, and survival rate. Based on the results of this study, it is concluded that the provision of planting media and shallot skin POC treatment affects the growth of moon orchids. The results of Duncan's test showed the M3P4 treatment combination (chopped fern + coconut shell) and shallot skin POC (30%) produced the best growth in the parameter of root length of 5.16 cm and plant weight of 2.82 grams. However, it has no effect on the number of leaves and leaf area. Keywords: Moon orchid; Growing media; POC; Chopped fern, coconut shell; Shallot skins.
Respon Pertumbuhan Tanaman Anggrek Bulan (Phalaenopsis amabilis) Terhadap Perlakuan Media Tanam dan POC Kulit Bawang Merah (Allium cepa) Putri Rahmawati, Andhini; Karunia Binawati, Diah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 17 No 01 (2024)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.17.01.9257.140-152

Abstract

The Kalimas River is a river that crosses industrial, commercial, office and densely populated residential areas in the city of Surabaya. Various activities in the area will produce waste that has the potential to pollute the Kalimas River. The indicator of polluted waters is the abundance of coliform bacteria and faecal coliforms. In some cases, the coliform and faecal coliform bacteria have become resistant to antibiotics which are currently of global concern. This study was conducted to determine the level of contamination of coliform bacteria and faecal coliforms in the Kalimas River and the level of resistance to several antibiotics. This study uses observational quantitative methods with sampling using purposive sampling and grab sampling techniques. River water samples were taken from 3 location points, namely the Kalimas River which crosses Jasa Tirta I Karah, Taman Prestasi and PT. Kasa Husada. Determination of the number and density of coliform and faecal coliform bacteria was carried out using the MPN method and testing of bacterial resistance to antibiotics using the disc diffusion method. Antibiotics used were amoxicillin, tetracycline, chloramphenicol and ciprofloxacin. Data were analyzed using ANOVA level 5% test and LSD and Duncan follow-up test. The results showed that the total bacterial density in all samples from each location in the Kalimas River exceeded the predetermined water body quality standard criteria. The highest average number of coliform and faecal coliform bacteria was found at location P3, namely 1.6 x 105 MPN / 100 ml. These coliform and faecal coliform bacteria have a 100% resistance level to amoxicillin antibiotics and have 100% sensitivity to tetracycline, chloramphenicol, and ciprofloxacin antibiotics. Ciprofloxacin is an antibiotic that shows the highest sensitivity rates. Keywords: Kalimas River, pollution, coliform, faecal coliform , antibiotic resistance
STUDI LITERATUR: STRUKTUR GENOME DAN IDENTIFIKASI GEN YANG BERPERAN DALAM KETAHANAN DEINOCOCCUS RADIODURANS TERHADAP RADIASI Anisa Oktobianti , Andi; Jamaluddin, Nurbina Septiani; Hala, Yusminah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 18 No 01 (2025)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.18.02.10135.%p

Abstract

Deinococcus radiodurans is an extremophilic bacterium with exceptional resistance to ionizing radiation, oxidative stress, and extreme environmental conditions. This resistance is supported by a unique multipartite genome structure, efficient DNA repair mechanisms, and complex global regulation. Key genes such as recA, ssb, pprA, and irrE play crucial roles in DNA repair and cellular protection. The bacterium can fully reconstruct its genome after severe radiation-induced damage, making it an ideal model for studying DNA repair and stress response mechanisms. Due to its remarkable resilience, D. radiodurans hold great potential for various applications, including radioactive waste bioremediation, radiation protection in medical fields, and advancements in biotechnology and genetic engineering. Further research is necessary to explore its molecular mechanisms and technological applications in industry, healthcare, and environmental sectors.
Thermus aquaticus: Keunikan Genetik dan Perannya dalam Revolusi PCR melalui Taq Polymerase Tenri Ampa Nurfitria Papada, Andi; Astuti S, Dewi; Hala, Yusminah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 18 No 01 (2025)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.18.02.10163.14-21

Abstract

Thermus aquaticus is a thermophilic bacterium known for its ability to produce thermostable enzymes, such as Taq polymerase, which plays an important role in the Polymerase Chain Reaction (PCR) technique. This study aims to examine the characteristics, ecology, and potential applications of thermostable enzymes produced by this bacterium in various industrial and biotechnological fields. The study was conducted through a literature review using a search method in scientific databases such as PubMed, Scopus, and Google Scholar. The results of the study showed that T. aquaticus has an optimal growth temperature between 70-75°C and is found in extreme environments such as hot springs and hydrothermal systems. Its characteristics, which include strong cell walls, unique membrane lipid content, and thermostable enzymes, allow this bacterium to survive in high temperature conditions. The Taq polymerase enzyme isolated from this bacterium has revolutionized the field of biotechnology, especially in PCR which is used for DNA amplification in genetics, medicine, and forensics research. In addition to PCR, thermostable enzymes from T. aquaticus have wide applications in the food, pharmaceutical, and bioremediation industries. Its existence in the ecosystem shows an important role in the biogeochemical cycle and its interaction with other microorganisms in extreme environments. This finding confirms that the exploration of extremophile microorganisms, including T. aquaticus, still has great potential in technological innovation and the development of biotechnology-based industries.
Antagonistic Activity of Pseudomonas fluorescens Against Curvularia sp. Causing Leaf Spot Disease in Maize Vivin Andriani; Purity Sabila Ajiningrum; Riza Putri Hanifah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 18 No 01 (2025)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.18.01.10320.%p

Abstract

Maize (Zea mays L.) is an important agricultural commodity in Indonesia, yet its production is often hindered by pathogenic infections, notably Curvularia sp., the causal agent of leaf spot disease. This study aimed to evaluate the antagonistic activity of Pseudomonas fluorescens against Curvularia sp. under in vitro conditions. The pathogenic fungus was isolated from infected maize leaves, while P. fluorescens was isolated from soil samples and identified through biochemical tests and pigment production on King’s B medium. Observations revealed that P. fluorescens produces fluorescent pigments, is an obligate saprophyte, and does not induce soft rot in potato tissue. Antagonism assays showed that the growth of Curvularia sp. was inhibited in the presence of P. fluorescens, as evidenced by fungal hyphae growing away from the bacterial colony. This inhibitory effect is likely due to antifungal secondary metabolites produced by P. fluorescens, including antibiotics, hydrogen cyanide, siderophores, and extracellular enzymes such as chitinase and cellulase, which degrade fungal cell walls. These findings suggest that P. fluorescens holds promise as a biocontrol agent for managing leaf spot disease in maize.   Keywords: Zea mays, Pseudomonas fluorescens, Curvularia sp., antagonism, leaf spot disease
EFEK PEMBERIAN INFUSA KOMBINASI TEMU IRENG, TEMULAWAK, KENCUR DAN JAHE TERHADAP BERAT MENCIT GALUR SWISS-WEBSTER Indira Pipit Miranti; Arinda Nur Cahyani; Eko Hidayaturohman; Ninstar Fathina Rifa
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 18 No 01 (2025)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.8.01.10354.%p

Abstract

Signs that children have difficulty eating and will then trigger malnutrition are very limited nutritional intake and a reduced appetite. Temu ireng, curcuma, sand ginger and ginger rhizomes contain essential oils, curcumin, xanthorizol, gingerol and flavonoids in each plant which can increase body weight and appetite. The purpose of this study was to determine the effect and optimal frequency of infusion combination on body weight and feed consumption of mice. The research was conducted in the Pharmacology Laboratory of STIKes Ibnu Sina Ajibarang. This type of research is true experimental with pre-post-test control design method. 28 male mice were divided into four groups. Group P0 (aquadest), group P1 (½ x dose), group P2 (1x dose) and group P3 (1 ½ x dose). All treatment groups were acclimatized for 7 days, then treated according to the predetermined dose, then observed body weight gain and feed consumption for 28 days. Data analysis was conducted using paired t-test, One Way ANOVA test, and Post-hoc LSD test. The results showed that there was a significant effect on body weight gain in group P2 (p=0.000) and feed consumption in all groups in the first, third and fourth weeks. (p<0,05).   Keywords; Effect, Body Weight, Infusa, Rhizome.

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