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Ivandini Tribidasari A.
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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 10 Documents
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Search results for , issue "Vol. 16, No. 3" : 10 Documents clear
Enzymatic Synthesis of Sucrose Polyester as Food Emulsifier Compound Handayani, Sri; Novianingsih, Ika; Barkah, Awaliatul; Hudiyono, Sumi
Makara Journal of Science Vol. 16, No. 3
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Sucrose polyester (SPE) is a carbohydrate ester compound that has diverse functions, from surfactant to low-calorie food products. Sucrose fatty acid ester with the degree of substitution 1-3 can be used as emulsifier in foods and cosmetics. The enzymatic synthesis of sucrose polyesters can be carried out using lipase in organic solvent and contain small amount of water. In this study sucrose esters were synthesized by esterification reaction between sucrose with fatty acids from coconut and palm oil using Candida rugosa lipase in n-hexane. Optimization esterification reaction was carried out for parameters of incubation time, temperature, and the ratio of the substrate. The optimum incubation time was at 18 hours for coconut oil and 12 hours palm oil, the optimum temperature was 30 o C for coconut and palm oil, and the mole ratio of fatty acid to sucrose was 40:1 for coconut oil and 64:1 for palm oil. Esterification products were characterized by FT-IR. The FT-IR spectrum showed the ester bond was formed as indicated by the wave number 1739.79/cm. Esterification products have 2 substitution degrees.
Photocatalytic Decolorization of Acid Red 4 Azo Dye by Using Immobilized TiO2 Microparticle and Nanoparticle Catalysts Sugiyana, Doni; Soenoko, Bambang; Handajani, Marisa; Suprihanto, Suprihanto
Makara Journal of Science Vol. 16, No. 3
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This research studied the synthesis of immobilized TiO2 microparticle and nanoparticle catalyst materials and evaluated its photocatalytic activity on the decolorization of Acid Red 4 (AR4) azo dye solution. TiO2 microparticles were immobilized on an acrylic plate, while TiO2 nanoparticles were immobilised on a glass plate. TiO2 nanoparticles were embedded in a nanofiber support layer to enhance photocatalytic performance, forming a nanofiber-nanoparticle composite on the glass plate. In the catalyst load 2.0 g/l, the performance of the photocatalytic layer of immobilized catalyst (k '= 0.013/min) was better than that of the suspended catalyst (k' = 0.008/min). Photocatalytic decolorization efficiency of TiO2 immobilized catalysts of nanoparticle sized reached 82.3% in irradiation time of 2 hours, which was higher than microparticle sized catalysts (77.8%). The technique of TiO2 nanofiber–nanoparticle composite showed improved photocatalytic kinetics (k’= 0.018/min) compared to a single layer of nanoparticle catalyst (k'= 0.015/min). Immobilized TiO2 catalysts remain effective with repeated use despite a slight decrease in processing efficiency.
The Effect of Alkaline Treatment to the Structure of ZSM5 Zeolites Octaviani, Savitri; Krisnandi, Yuni K.; Abdullah, Iman; Sihombing, Riwandi
Makara Journal of Science Vol. 16, No. 3
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Adsorption of Waste Metal Cr(VI) with Composite Membranes (Chitosan-Silica Rice Husks) Zulti, Fifia; Dahlan, Kiagus; Sugita, Purwantiningsih
Makara Journal of Science Vol. 16, No. 3
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Chromium compounds are widely used in modern industry. Many of these compounds are dumped into the surrounding environment. Membrane technology is more efficient and effective than conventional methods for waste treatment. The research objective was to make a membrane separation process that can be applied to Cr(VI). Membranes were made from chitosan and silica rice husks. Variations of chitosan and silica rice husk used (g) are 2:1 (A1), 2:2 (A2), 3:1 (B1), and 3:2 (B2). The membrane was made by using an inverted phase technique. Results of SEM characterization of membranes showed that B2 has the largest pores at 2.58 μm. The FTIR characterization results indicate the presence of crosslinking between chitosan with silica rice husk with the appearance of Si-O adsorption band at wavelength 1122- 980/cm. A1 membrane, with the smallest pore size has the greatest rejection value towards Cr(VI) which is 70%. The result of this research showed that the composite membrane of silica rice husk was effective enough to adsorb metal Cr(VI) with an average adsorption capacity of 1665.85 mg/g.
Investigation on the Synergistic Complexation of Ni(II) with 1,10-Phenanthroline and Dithizone at Hexane-Water Interface Using Centrifugal Liquid Membrane-SpectrophotometryInvestigation on the Synergistic Complexation of Ni(II) with 1,10-Phenanthroline and Dithizone at Hexane-Water Interface Using Centrifugal Liquid Membrane-Spectrophotometr Yulizar, Yoki; Wahyuningsih, Nerny; Asri, Novena Damar; Watarai, Hitoshi
Makara Journal of Science Vol. 16, No. 3
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Complex formation of Ni(II) and 1,10-phenanthroline (C12H8N2/Phen) with the addition of dithizone (C13H12N4S/HDz) at the hexane-water interface has been studied by direct measurement spectrophotometry using the centrifugal liquid membrane (CLM) method. Ni(II) ion with Phen formed a cationic complex of Ni(C12H8N2)2 2+ or NiPhen2 2+. That complex dissolved in the aqueous phase and had two UV absorption spectrum maxima wavelengths, λmax 270 and 292 nm. Observation of complex formation was performed variations of pH and ligand concentration. The pH caused protonation that affected the amount of the formed complex. With the variations of ligand concentrations, the greater was the concentration of ligands the greater was the formed complex. Based on the Batch method, the HDz ligand addition into the NiPhen2 2+ cationic complex produced ion association complex of Ni(C13H11N4S)2(C12H8N2) or NiDz2Phen at λmax 403 nm, and was extracted in the organic phase. Measurement results using CLM method showed that NiDz2Phen complex was formed at hexane-water interface with λmax 523 nm. Comparison of Phen with HDz ligand concentrations affected the initial formation rate of NiDz2Phen complex. The greater concentration of Phen ligand increased the initial rate of formation for synergistic complex. The obtained data using CLM method indicated that the synergistic complex formation rate constant of NiDz2Phen at the interface, k was 0.30/s.
Effects of Methanolic Jatropha multifida L. Extract in Wound Healing Assessed by the Total Number of PMN Leukocytes and Fibroblasts Juniarti, Juniarti; Aryenti, Aryenti; Yuhernita, Yuhernita; Poerwaningsih, Ernie Ernie
Makara Journal of Science Vol. 16, No. 3
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The aim of this study was to evaluate the effects of methanol extract of Jatropha multifida leaves on the wound healing process and to investigate the wound healing activity based on reduced numbers of PMN (polymorpho nuclear) leukocytes and increased numbers of fibroblasts. Methanol extract of dried leaves of J. multifida was used in the wound healing activity studies. The study subjects were 36 white male Sprague Dawlay rats aged 2 months with 150-200 gram body weight. The subjects were divided into 4 groups and experimentally injured: Group I (negative control) underwent injury without subsequent treatment; group II (positive control) received topical treatment with Bethasone-N after injury; group III (solvent control) was treated with 70% methanol; group IV (treatment group) was treated with 10 mg methanol extract of J. multifida Each group consisted of 3 rats, which were decapitated on days 3, 6, and 13 after the start of treatment. Histological preparation was stained with hematoxyline-eosin (HE) and was continuously examined by counting the numbers of PMN leukocytes and fibroblasts as indicators of wound healing on days 3, 6, and 13 of treatment. The study showed lower numbers of PMN leukocytes in subjects treated with the extract of J. multifida as compared to the other groups. The numbers of fibroblasts were significantly higher on days 6 and 13 of treatment. The treatment of injuries with methanol extract of leaves from J. multifida provided better results compared to the other groups in our study.
The Relationship between the Harmful Algal Blooms (HABs) Phenomenon with Nutrients at Shrimp Farms and Fish Cage Culture Sites in Pesawaran District Lampung Bay Hasani, Qadar; Adiwilaga, Enan Mulyana; Pratiwi, Niken Tunjung Murti
Makara Journal of Science Vol. 16, No. 3
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The phenomenon of harmful algal blooms (HABs) in the Lampung Bay has been reported by many researchers. The occurrence of HABs may be due to the increase of nutrient (N and P) as results of waste water of aquaculture (shrimp farms, hatcheries and fish cage farms). This study aimed to determine the relationship between N and P concentrations in some aquaculture sites with harmful algal blooms. The analysis revealed the differences concentration of N and P at each different shrimp farms and fish cage farms sites (Hurun, Sidodadi, Ringgung, and Cikunyinyi Bay). The result showed that the increase of N and P concentration were followed by the increase of harmful phytoplankton populations. High density HABs were found in this study, such as: Ceratium furca with the highest density at 5.314 x 106 cells/l, Trichodesmium erithraeum 1.05 x 105 cells/l and Noctiluca scintilans 5.99 x 104 cells/l. The Multiple regression and canonical corelation analysis (CCA) also indicated a strong positive relationship between N and P with the HABs at the shrimp farms and fish cage farms sites in the Lampung Bay.
Anti-Malaria Study of Nigella sativa L. Seed Water Extract in Mus musculus Mice Balb C Strain In Vivo Sosiawan, Tunru Insan; Linda, Weni; Etty, Widyantia
Makara Journal of Science Vol. 16, No. 3
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Nigella sativa L. has been reported to exhibit many pharmacological effects, including anti-parasitic properties. This study investigated the anti-malarial effects of a water extract of N. sativa seed in Mus muculus mice infected with the Plasmodium berghei NK65 parasite. The method used was to take a blood parasitaemia count, following the use of Giemsa dye, determining the level of nitric oxide in mice that were infected with P. berghei malaria, using the spectrophotometric method, and determining their survival rate after 20 days of being infected with P. berghei malaria. The results showed that the decrease in the number of parasitaemia and the level of nitric oxide in subjects treated with doses of N. sativa was significant (p < 0.05). Further results showed that P. berghei-infected mice that were given 100 µg/kg of body weight had a better chance of survival. The conclusion is that the provision of N. sativa may reduce the number of malaria parasites and reduce levels of NO. The decrease in the number of parasites may be caused by an immune mechanism, through the regulation of NO levels (lower levels of NO), due to the influence of the anti-oxidant effects of N. sativa. Survival rates of the mice did not show significant results with reduced levels of parasitaemia and NO. This is likely to be because the levels of NO in this group were below the threshold levels at which NO can function as an anti-parasitic. It is alleged that, while NO can function as an anti-parasitic at certain levels, at lower levels its function as an antiparasitic is not optimal. On the other hand, if the levels are too high, damage will result, because of the nature of free-radicals.
Vertical Distribution and Flux of Nutrients in the Sediments of the Mangrove Reclamation Region of Muara Angke Kapuk, Jakarta Purwiyanto, Anna Ida Sunaryo; Prartono, Tri; Koropitan, Alan Frendy
Makara Journal of Science Vol. 16, No. 3
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The reclaimed mangrove estuary in Muara Angke Kapuk is a reclaimed area that has not evaded the impacted of pollution and waste in the areas surrounding Cengkareng, Jakarta. This is apparent from the fact that almost all sediments under the mangrove trees are buried under heaps of plastic trash. However, the reclaimed region still has variety of organism, which indicating that the region still has an internal carrying capacity, especially nutrients from sediment. The purpose of this research was to examine the condition of sediment nutrients in this mangrove reclamation region. The research was conducted by taking water samples using a modification of the stratified cup at a sediment depth of 0-15 cm with depth intervals of 2.5 cm, and taking sediment samples using the sediment ring. Pore water samples were measured for dissolved oxygen (DO) and concentrations of ammonia, nitrite, nitrate, and phosphate. Sediment samples were used to obtain porosity values. The data obtained is used to make vertical concentration profiles and analysis of vertical nutrient flux. Vertical nutrient flux analysis was performed with the aid of QUAL2K software version 2.11. The results showed different vertical distributions and flux of nutrients, where influx for ammonia and phosphate and an increase in line with increasing sediment depth, while nitrate efflux and a decreased concentration. The flux calculation of nitrite as transitory nutrient was not done, but the concentration decreased after a depth of 2.5 cm. This indicates that the high contamination on the surface does not prevent the natural chemical processes so the reclaimed region can still provide nutritional support for its organism.
Phylogeny of Indonesian Nostoc (Cyanobacteria) Isolated from Paddy Fields as Inferred from Partial Sequence of 16S rRNA Gene Hendrayanti, Dian; Kusmadji, Lestari Rahayu; Yuliana, Pratiwi; Amanina, Mardhotillah Asma
Makara Journal of Science Vol. 16, No. 3
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In order to collect Indonesian Nostoc, isolation of soil microflora from several paddy fields in West Java, Bali, and South Celebes was carried out. Fast-growing isolates of Nostoc were selected to describe and perform molecular identification using partial sequences of 16S rRNA. The results showed that partial sequences of 16S rRNA could not resolve the phylogeny of the isolates. However, it supported the morphological studies that recognize isolates as different species of Nostoc. Potential use of Nostoc as a nitrogen source for paddy growth was carried out using six strains as single inoculums. A total biomass of 2 g (fresh weight) for each strain was inoculated, respectively, into the pot planted with three paddy plants. This experiment was conducted in the green house for 115 days. Statistical analyses (ANOVA; α = 0.05) showed that of six strains tested in this study, only strain GIA13a had influence on the augmentation of root length and the total number of filled grains.

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