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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 18 Documents

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Search results for , issue "Vol 34, No 2 (2016): Desember" : 18 Documents clear

Ancaman terhadap Masuknya Virus Penyakit Mulut dan Kuku melalui Daging Ilegal di Perbatasan Darat Indonesia-Malaysia Risma Juniarti Silitonga; Retno Damayanti Soejoedono; Hadri Latif; Etih Sudarnika
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Penelitian ini bertujuan untuk menganalisis keberadaan daging ilegal di perbatasan darat Indonesia-Malaysia sebagai ancaman risiko masuknya virus PMK ke wilayah Indonesia. Data primer diambil menggunakan teknik pengumpulan pendapat pakar dengan kuisioner, wawancara mendalam (in-depth interview) dan pengamatan langsung di lapang. Data sekunder diperoleh dari publikasi ilmiah dan tulisan atau data yang tidak dipublikasi (statistik, dokumen dan laporan dari instansi berwenang). Penentuan responden secara purposive sampling. Hasil studi menunjukkan bahwa daging ilegal diperkirakan berasal dari berbagai negara termasuk dari negara/zona yang berstatus endemis PMK seperti Semenanjung Malaysia, Thailand, India dan negara/zona yang dinyatakan tidak diketahui oleh responden. Jenis daging ilegal yang masuk ke Entikong berisiko sebagai sumber infeksi PMK seperti daging beku bertulang tanpa limfoglandula dan jeroan beku tanpa limfoglandula. Berdasarkan jalur dan frekuensi pengangkutan, perkiraan volume pemasukan daging ilegal menunjukkan kemungkinan daging masih bisa lolos melalui jalur non-kendaraan. Kondisi-kondisi tersebut mengindikasikan bahwa pemasukan daging ilegal dapat sebagai ancaman risiko masuknya virus PMK ke Indonesia khususnya di perbatasan darat Indonesia-Malaysia, Entikong. Perlu dilakukan upaya pencegahan untuk mengurangi ancaman risiko yaitu dengan melakukan pengawasan yang lebih ketat di pintu-pintu pemasukan dengan koordinasi lintas instansi di perbatasan untuk bersama-sama mencegah pemasukan daging ilegal.

PENGARUH PEMBATASAN PAKAN DAN PEMENUHAN KEMBALI PAKAN TERHADAP KONSUMSI NUTRIEN KECERNAAN NUTRIEN, STATUS FISIOLOGI DAN PERUBAHAN BERAT BADAN KAMBING BLIGON Bambang Suwignyo; Ulil Amri Wijaya; Rieska Indriani; Asih Kurniawati; Irkham Widiyono; Sarmin Sarmin
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.11603

Penelitian ini bertujuan untuk mengetahui kecernaan nutrien pakan dan status fisiologi pada kambing Bligon yang diberi perlakuan pembatasan pakan (feed restriction). Enam ekor kambing Bligon jantan umur rata-rata 12 bulan dengan bobot badan rata-rata 23,6 kg diberi pakan yang terdiri dari hijauanjerami kacang tanah (rendeng)dan konsentrat. Kambing dibagi menjadi 2 kelompok perlakuan. Tiga ekor kambing untukperlakuan kontrol diberi pakan berdasarkan kebutuhan bahan kering (BK) 3,5% dari bobot badan, dan tiga ekor kambing untuk perlakuan pembatasan pakandilakukan pembatasan pakan dengan pengurangan pakan 50% dari kebutuhan bedasarkan BK selama 35 hari. Variabel yang diamati adalah konsumsi bahan kering (BK), konsumsi bahan organik (BO), konsumsi total digestible nutrients (TDN), perubahan berat badan, kecernaan bahan kering (BK), kecernaan bahan organik (BO), temperatur tubuh, frekuensi respirasi, dan frekuensi detak jantung. Data yang diperoleh dianalisis dengan independent samplesT-Test. Hasil penelitian yang telah dilakukan, dapat disimpulkan bahwa pembatasan pakan (feed restriction) berpengaruh nyata terhadap penurunan konsumsi bahan kering (BK), konsumsi bahan organik (BO), konsumsi total digestible nutrients (TDN), kecernaan bahan kering (BK) dan bahan organik (BO), tetapi tidak berpengaruh nyata pada temperatur tubuh, frekuensi respirasi, dan frekuensi detak jantung (pulsus) kambing Bligon. Pemenuhan kembali (refeeding) pakan berpengaruh nyata (P<0,05) menaikan kembali konsumsi BK dan konsumsi BO. Perubahan berat badan kambing mengikuti pola jumlah pemberian pakan. Saat pembatasan pakan, berat badan kambing perlakuan turun 55,24 g/hari dan ketika pakan dipenuhi kembali berat badannya meningkat 131,43 g/hari.

Ancaman Masuknya Virus Penyakit Mulut dan Kuku Melalui Daging Ilegal di Entikong, Perbatasan Darat Indonesia dan Malaysia Risma JP Silitonga
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study was aimed to analyze the risk of FMD virus to enter Indonesia by the presence of illegal meat at Entikong, a borderland between Indonesia and Malaysia. The primary datas were collected using questionnaires method to collect expertise, in-depth interviews, and direct field observations. Secondary datas were obtained from scientific publications and articles or unpublished datas (statistics, goverment documents and reports). The respondents were choosen by purposive sampling. The circulating illegal meat was possibly originate fromdifferent countries, including FMD-endemic countries/zones such as Peninsular Malaysia, Thailand, India and even unidentified countries/zones according to our respondends. The types of illegal meat such as frozen deglanded bonein-meat and frozen deglanded offal that enter Entikong carry the risk of becoming a source of FMD infection. Based on the entry route, delivery frequency, and meat volume, ilegal meat was possibly also entered into Indonesia using non-vehicular transportation. These conditions indicated that the entrance of illegal meat could pose risk of the FMD virus entry to Indonesia, especially at the borderland between Indonesia and Malaysia at Entikong. It is highly recommended to apply strict inspection at the border entraces and increase collaboration with related institutions to prevent the entrance of illegal meat and to reduce the entry risk of FMD virus.

Pengembangan Metode Identifikasi Kerusakan DNA Spermatozoa Ternak Teguh Ari Prabowo; R. Iis Arifiantini; Dondin Sajuthi; Uus Saefullah
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The success of artificial insemination is very much determined by the quality of spermatozoa. The detection or identification of damaged chromatin of spermatozoa DNA is very important to forsee the adverse clinical outcome. However, the method of identification is still depended on expensive imported kits. Therefore, the objective of this research was to developed an identification kit to determine the quality of livestock spermatozoa DNA chromatin.This study consist of three step. Step 1) Determination of low melting point agarose (LMP-agarose) concentration which is 0,6%, 0,7% and 0,8%. 2) Comparison of three lysis solution (LS) which is LS I (0.4M Tris, 0.8M DTT, 1% SDS, pH 7.5), LS II (0.4M Tris,2 M NaCl, 1% SDS , pH 7.5), and LS III (0.4M Tris-HCl, 2M NaCl, 1% SDS 0,05 M EDTA, pH 7.5). 3) Comparison different staining which is Eosin yellow and Methylene blue. The results showed that 0.6% LMP-agarose demonstrated the best concentration to “trapped the spermatozoa” compared for sheep and goats. whereas the three concentration of spermatozoa cows can not be used to trap spermatozoa cow. The best formulation to lysis the membrane was LS III (0.4M Tris -HCl, 2M NaCl, 1% SDS 0,05 M EDTA). The best staining was eosin yellow and methylene blue with 2:1 ratio.

Peran Makromineral pada Reproduksi Ruminansia Yanuartono Yanuartono; Soedarmanto Indarjulianto; Alfarisa Nururrozi; Hary Purnamaningsih
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Macromineral is one component of a nutrient that has an important role in the growth, health, production,reproduction and immune system of animals. Ruminants need makromineral such as Ca, P, Mg, K, Na , Cl and S. Mineral needs of ruminant affected by several factors such as their age, pregnancy and lactation status.Mineral deficiency can cause disturbances in reproduction ruminant. Ca and P have direct influence while Mg, K, Na, Cl and S acted indirectly on reproductive function. A complete understanding of the role macromineral on ruminant reproductive function is indispensable for the prevention of their reproductive disorders due toimproper feeding minerals.

Deteksi Spesies Brucella pada Kambing di Rumah Potong Hewan Jakarta Mujiatun Mujiatun; Retno Damajanti Soejoedono; Etih Sudarnika; Susan Maphilindawati Noor
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Brucellosis is a zoonosis and occupational diseases transmision. The diseases caused by bacterial and attack multiple species of animals. Common species that infects goats as the most pathogenic species (zoonotic) is Brucella melitensis; however, the species B. abortus could also infect goats. The study purposed to find out the brucellosis seropositive in goat in Jakarta slaughterhouse and to detect caused agent of brucellosis. Sampling was done through slaughtered goats that come from brucellosis endemic area. The samples were collected fromslaughtered mature female goats i.e serum, goat milk, vaginal swab, mamary gland, limphoglandula supramamary, limph, and uterus. The detection method was used i.e patological lession, serological, culture and PolymeraseChain Reaction (PCR) technique. The serological detection of brucellosis in goats was done parallelly between Rose Bengal Test (RBT), Complement Fixation Test (CFT) and Enzyme Linked Immunosorbent Assay (ELISA). The results of this study demonstrated that out of the 119 serum samples serologically tested, negative for RBT, one was positive for CFT and none were positive with ELISA. Patological observation in the Brucella predilection organs, there were 5 goat carcases showed pathological lession (vagina discharge, hemoragy at limphand limphoglandula, crumbly limph and there were pus in uterus). The serum samples that had reacted positively and the organs with pathological lesion were confirmed further with PCR, bacterial isolation and identification.The PCR test results and the culture of milk samples, vaginal swabs and organs did not reveal any Brucella spp bacteria (B. abortus, B. melitensis, B. ovis dan B. suis) and also vaccine strains of RB51. Based on these results, it was concluded that brucellosis in goats on Java Island was a 0.84% seropositive (confidence interval 95%; 0.00826 - 0.00854) (1/119), although the species of Brucella that had infected them remains unknown.

Efek Antikariogenik Ekstrak Daun Beluntas (Pluchea indica) sebagai Penghambat Pertumbuhan Streptococcus Mutans penyebab Karies Gigi Nungki Fatimatuzzahra; Nourmalita Safitri Ningsih; Feny Feny; Adam Darsono; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Anti-plaque agents are known to reduce the formation of dental biofilms such as fluoride and chlorhexidine. However, excessive use of fluoride causes fluorosis and chlorhexidine side effects caused genotoxic. Pluchea indica L is one of the abundance of tropical herbs in the Asia-Pacific reported to have antiinflammatoryproperties and reduces the incidence of gastric necrosis, antinociception, neutralizing toxic venom, antioxidant, antiulcerative, hepatoprotective, antimoeba and broad-spectrum antimicrobial. This study examinesthe effectiveness anticariogenic Pluchea indica leaf extract in inhibiting the growth of Streptococcus mutans causing dental caries in vitro. Determination of Minimum Inhibitoric Concentration (MIC) on Pluchea indica leafextract is by diffusion test on the growth of Streptococcus mutans by Kirby Bauer Test. S. mutans concentration Mc Farland 0.5 standard (108 CFU ml-1) were inoculated in media Müller-Hinton agar (MHA). Paper diskcontaining Pluchea indica leaf extract at each concentration of 10%, 20%, 30%, 40%, 50%, 60%, and 70% were placed in an agar medium inoculated bacteria then incubated at 37°C on condition microaerophilia using candlejar. Determination of significance various Pluchea indica leaf extract concentration using one-way ANOVA test. The result of the diffusion method showed Pluchea indica leaf extract at a minimum concentration of 10% beenable to establish inhibited zone of Streptococcus mutans growth in MHA media. By one-way ANOVA test to various concentrations of extracts using Microsoft Excel 2007 have been obtained with F=4.85, Fcrit = 2.85 andP-value = 0.0071. Based on this research, it was concluded that Inhibitoric Minimum Concentration Pluchea indica leaf extract is capable of effective concentration of 10% and it has a significant (P-value< 0,05) of variousconcentration.

Sonogram Pemeriksaan Kebuntingan Dini pada Kambing Kacang (Capra hircus) Santoso Santoso; Amrozi Amrozi; Bambang Purwantara; Herdis Herdis
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study was conducted to determine the earliest day of pregnancy diagnosis in kacang goat using transrectal ultrasonography. The goat were synchronized by using prostaglandin in the luteal phase. Pregnancy was determined by isoechogenic visualization surrounded by hypoechogenic. Early pregnancy was detected on days 20 of embryonic vesicle diameter 1.2±0.1 cm. Fetus was detected on days 22 with a long gestation fetus 0.4±0.1 cm. Average increase until days 30 pregnancy was 0.19 ± 0.1 cm per day. Development of fetus was followed by an increasing the diameter and thickness of uterus. The diameter of uterus increased from days 14 (0.8 ± 0.3 cm) until days 30 (3.6 ± 0.2 cm), and thickness of uterus increased from days 14 (0.4 ± 0, 2 cm) until days 30 (1.8 ± 0.2 cm). It could be concluded that the earliest pregnancy diagnosis showed positive sign on days 20 and fetus was earliest observed on days 22.

Studi Respon Imun Humoral Mencit, Tikus, dan Ayam terhadap Infeksi Toxoplasma Gondii Gifti Rosalina Ratnaningrum; Dana Meida; Andhini Mutiara Putri; Hilda Syara Shita Devi; Dwi Priyowidodo
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Toxoplasmosis is a disease caused by the protozoan Toxoplasma gondii. T. gondii is an obligate intracellular parasite that is transmitted by congenital and acquisition. T. gondii can infect all warm-blooded animals and are zoonotic. It is estimated that more than a third of the world’s population is infected by T. gondii. Chicken is one of the animals that can be infected with T. gondii and plays an important role in the spread of this disease to humans because it is one of the animals used as a source of animal feed. Mice and rats are also play an important role in the spread of toxoplasmosis in the definitive host, especially in cats. T. gondii infection is usually asymptomatic, but in certain circumstances, such as when the host’s immune system is weakened, this parasite can causeserious illness. This study were aimed to determine the humoral immune response against T. gondii infection in mice, rats and chickens by serological Latex Agglutination Test (LAT) using Pastorex-Toxo® kit. Two weeks oldbroiler chickens were 8 heads, Wistar strain rats aged 8 weeks as many as 18 heads and strain Balb C mice aged 8 weeks as many as 13 cows used in this study. Animals were grouped into two groups, namely the control andinfection groups. Mice, rats, and chickens were infected each with 103, 107 and 105 takizoit by intra-peritoneal injection. Blood samples were taken from the animals and infection control from day to-1, 2, 3, 4, 5, 6 for mice,the to-1, 2, 3, 4, 5, 6, 7, 8 for rats, and day - 2, 4, 14 for chicken by intracardia 3 ml, then put in a non-EDTA tubes and centrifuged at a speed of 10,000 rpm for 5 minutes to collect serum. The results showed a humoral immuneresponse against T. gondii in rats began to appear on day 5 post-infection, chickens began to emerge the 2nd day post infection, whereas mice do not indicate a humoral immune response against T. gondii post infection.

Potensi Ekstrak Daun Sage (Salvia officinalis.L) sebagai anti-Streptococcus suis Penyebab Zoonotik Meningitis Mitra Slipranata; Fajar Budi Lestari; Novra Arya Sandi; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The phenomenon of microbial resistance to an antibiotic or some kind of specific antibiotics (multi drugs resistance) greatly complicate the treatment process, one of which is Streptococcus suis (S. suis) which is known to cause meningitis in animals and humans. Today, with rising bacterial resistance to a wide rangeof antibiotics, it takes an effort to assess the potential medicinal plants as an antibiotic that is appropriate and safe. Sage (Salvia officinalis.L) is reported to have antibacterial and fungicidal effect. Phenolic acids such assalvin and ether monomethyl salvin which isolated from the sage thought to have antimicrobial activity against several bacterial strains. The purpose of this study was to prove the ability of sage leaf extract as an antibacterialagainst S. suis causes streptococcal meningitis in vitro. In-vitro method used in this research through a two-stage dilution test and the diffusion test on Mueller Hinton Agar (MHA). S. suis isolates (code 225) were tested in vitro against 8 levels sage leaf extract concentration, ie a concentration of 1%, 3% 5%, 7%, 10%, 20%, 40% and 60%. The results of the test obtained by minimum inhibitory concentrations (MICs) and minimum sage known to potentially inhibit the growth of S. suis.

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