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INFLUENCES OF INCUBATION TIME AND SUCROSE CONCENTRATION ON MICE (Mus musculus L.) OOCYTE VIABILITY FOR ENUCLEATING PROCEDURE Ekayanti Mulyawati Kaiin; Muhammad Gunawan; Madihah Madihah; Ghina Nafisah
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

This study aimed to find out the optimum incubation time to complete mouse oocyte maturation at Metaphase II (MII) stage and determine the optimum sucrose concentration enabling to induce nuclear swelling for visualization that is important for enucleating process at the initial procedure of somatic cell nuclear transfer (SCNT). In this current study, mice were used as animal model. Completely randomized design was arranged, consists of 2 trials with 4 treatments and 7 replications. In the first trial, the oocytes were cultured at 0-2, 4-6, 8-10, and 12-14 h in 5% CO2 incubator at 37 C. Second, the MII oocytes obtained from previous trial were cultured in M199 medium containing different concentrations of sucrose (0, 1.5, 3, and 6%). The parameters measured were the oocyte viability at various stages, i.e germinal vesicle (GV), metaphase I (MI), anaphase/telophase I (A/T I), and metaphase II (MII), and the viability of swollen nuclear oocytes using Hoechst/PI staining. The results showed that the optimum incubation time required by oocytes to reach MII stage was 12-14 h with a percentage of 57.14±12.67%, while the optimum sucrose concentration for nuclear swelling was found at 3% with a percentage of 100±0.00%. Our findings provided preliminary results related to the maturation process of the mouse oocyte nucleus, which is meaningful for the initial procedure of SCNT.

THE COMPOSITION OF SATURATED AND UNSATURATED FATTY ACIDS AND CHOLESTEROL IN MEAT OF BALI BULL FATTENED USING GREENLOT SYSTEM IN SMALL HOLDER FARMING Paulus Klau Tahuk; Agustinus Agung Dethan; Stefanus Sio
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

The objective of this research was to analyze the composition of saturated fatty acid, unsaturated fatty acids, and cholesterol in meat of Bali bull fattened using forage crops in small holder farming. The experiment was conducted for 3 months using nine Bali bull aged 2.5-3.5 years old with the initial body weight of 227-290 kg in the feedlots of Bero Sembada Farmers Group, Laen Manen Sub District, Malaka Regency, East Nusa Tenggara. The experiment was conducted based on the fattening system of local farmers in terms of feeding, housing, and health management. Feed given during the experiment was Centrosema pubences, Clitoria ternatea, fresh corn straw, king grass, Leucaena leucocepala, natural grass, Pennisteum purpureum, and Sesbania grandiflora. At the end of this study, cattle were slaughtered and the composition of saturated fatty acid, unsaturated fatty acids and cholesterol were measured. Data were analyzed using a descriptive statistic. Results revealed that the total of saturated fatty acid and unsaturated fatty acids in meat were 36.628% and 26.739%, respectively, while the cholesterol reached 79.18 mg/100 g of meat. In conclusion, meat of Bali bull fattened using forage crops in small holder farming has higher saturated fatty acid than unsaturated fatty acids.

HISTOMORPHOMETRY OF SEMINIFEROUS TUBULES OF LOCAL GOATS FIXED WITH DIFFERENT FIXATIVES Fitriani Fitriani; Sri Wahyuni; Muslim Akmal; Dasrul Dasrul; Cut Dahlia Iskandar
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

The aim of this study was to further understand the histomorphometry of seminiferous tubules from local goat testicles that were fixed with different fixatives. Nine testicles were randomly chosen, each fixed with 10% formalin, 10% neutral buffered formalin (NBF), and 4% paraformaldehyde (PFA) solutions. The sample was then proceeded to histology preparation and stained with haematoxylin eosin (HE). Observation of the tissue was carried out using microscope and the data obtained was analyzed statistically. The results showed that there was a significant difference (P0.05) on each fixative in tubular diameter and membrane, and very significant difference (P0.01) on germinal cell and staining absorbability. Duncan test result on tubular diameter showed that the use of PFA fixative was significantly different (P0.05) compared to formalin fixative while the use of NBF fixative had no significant difference (P0.05) compared to PFA fixative. In addition, the measurement of interstitial membrane of seminiferous tubules showed no significant difference (P0.05) among three fixatives. To conclude, formalin, NBF, and PFA fixatives affect seminiferous tubules, basal membrane structure, and germinal cell appearance inside tubular lumen. Type of tissues and fixative must be considered in selecting the suitable fixative.

IMPROVING EWE OOCYTE VIABILITY AFTER VITRIFICATION WARMING USING COMBINATION OF DIFFERENT CRYOPROTECTANT AND CARRIER SYSTEM Rini Widyastuti; Rangga Setiawan; Mas Rizky Anggun Adipurna Syamsunarno; Mohammad Ghozali; Takdir Saili; Arief Boediono
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

The aim of this study was to determine the best combination of cryoprotectant (Ethylene glycol, EG), dimethyl sulfoxide (DMSO) and propanediol (PrOH) and carrier system (hemistraw and cryotop) in improving ewe oocytes viability during cryopreservation. Oocytes with multi layers of compact cumulus cells were colleted from abbatoir and matured in TCM 199 medium supplemented with 10% fetal calf serum for 24-26 h at 38.5° C under 5% CO2 in the air. Matured oocyte was divided into six parts and vitrified in three different vitrification solutions; (i) 17% EG+17% DMSO with hemistraw as carrier system, (ii) 34% EG with hemistraw as carrier system, (iii) 17% EG+17% PrOH in hemistraw (iv), 17% EG+17% DMSO with cryotop as carrier system (v), 34% EG with cryotop as carrier system (vi), and 17% EG+17% PrOH in cryotop. Oocytes were cryopreserved for one week before revived and evaluated for viability. The result showed that oocytes vitrified in media containing EG and DMSO in cryotop had the highest viability (88.16%) compared to media containing EG only or EG and PrOH (70.95% and 68.76%, respectively) (P0.05). Moreover, oocytes viability that vitrified using cryotop and hemistraw as carrier system were not significantly different. The present results indicated that vitrification using combination of EG and DMSO as permeable cryoprotectant and cryotop as carrier system was the best system to maintain oocyte viability after vitrification-warming.

MORPHOLOGY AND MORPHOMETRY OF Haemonchus contortus IN GOATS IN YOGYAKARTA, INDONESIA Priyo Sambodo; Joko Prastowo; Sudarmanto Indarjulianto; Kurniasih Kurniasih
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

This research was carried out to determine the morphology and morphometry of Haemonchus contortus in goats. Adult female parasites were collected from abomasum of naturally infected goats and stored in lactophenol. Images of the parasite were acquired using lucida camera and measurement was performed using Axiovision LE software. Basic characters of the morphology and morphometry of Haemonchus spp. were identified by determining the value of the discriminant function of three parameters on spicule. Vulvar morphology was identified by vulvar flap shape. Data were presented in terms of mean, standard deviation, and percentage. A hundred percent of the parasites were H. contortus with discriminant function value 0.63. The size of female parasites was longer and wider (25.5-32.6 mm; 0.38-0.63 mm) than that of male ones (17.3-20.0 mm; 0.24-0.33 mm). There were only 2 varieties of vulvar flap, linguiform (81%), and knobbed (19%). There were 5 subclasses identified in the linguiform type, including linguiform A (39%), linguiform B (22%), linguiform C (17%), linguiform I (1%), and a new subclass of linguiform D (2%). Based on the the type of vulvar flap, H. contortus found in goats in Yogyakarta were different to those found in previous researches. The findings could be used in identifying the parasite species in small ruminants.

INFLUENCES OF INCUBATION TIME AND SUCROSE CONCENTRATION ON MICE (Mus musculus L.) OOCYTE VIABILITY FOR ENUCLEATING PROCEDURE Kaiin, Ekayanti Mulyawati; Gunawan, Muhammad; Madihah, Madihah; Nafisah, Ghina
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i3.10896

This study aimed to find out the optimum incubation time to complete mouse oocyte maturation at Metaphase II (MII) stage and determine the optimum sucrose concentration enabling to induce nuclear swelling for visualization that is important for enucleating process at the initial procedure of somatic cell nuclear transfer (SCNT). In this current study, mice were used as animal model. Completely randomized design was arranged, consists of 2 trials with 4 treatments and 7 replications. In the first trial, the oocytes were cultured at 0-2, 4-6, 8-10, and 12-14 h in 5% CO2 incubator at 37 C. Second, the MII oocytes obtained from previous trial were cultured in M199 medium containing different concentrations of sucrose (0, 1.5, 3, and 6%). The parameters measured were the oocyte viability at various stages, i.e germinal vesicle (GV), metaphase I (MI), anaphase/telophase I (A/T I), and metaphase II (MII), and the viability of swollen nuclear oocytes using Hoechst/PI staining. The results showed that the optimum incubation time required by oocytes to reach MII stage was 12-14 h with a percentage of 57.1412.67%, while the optimum sucrose concentration for nuclear swelling was found at 3% with a percentage of 1000.00%. Our findings provided preliminary results related to the maturation process of the mouse oocyte nucleus, which is meaningful for the initial procedure of SCNT.

THE COMPOSITION OF SATURATED AND UNSATURATED FATTY ACIDS AND CHOLESTEROL IN MEAT OF BALI BULL FATTENED USING GREENLOT SYSTEM IN SMALL HOLDER FARMING Tahuk, Paulus Klau; Dethan, Agustinus Agung; Sio, Stefanus
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i3.10068

The objective of this research was to analyze the composition of saturated fatty acid, unsaturated fatty acids, and cholesterol in meat of Bali bull fattened using forage crops in small holder farming. The experiment was conducted for 3 months using nine Bali bull aged 2.5-3.5 years old with the initial body weight of 227-290 kg in the feedlots of Bero Sembada Farmers Group, Laen Manen Sub District, Malaka Regency, East Nusa Tenggara. The experiment was conducted based on the fattening system of local farmers in terms of feeding, housing, and health management. Feed given during the experiment was Centrosema pubences, Clitoria ternatea, fresh corn straw, king grass, Leucaena leucocepala, natural grass, Pennisteum purpureum, and Sesbania grandiflora. At the end of this study, cattle were slaughtered and the composition of saturated fatty acid, unsaturated fatty acids and cholesterol were measured. Data were analyzed using a descriptive statistic. Results revealed that the total of saturated fatty acid and unsaturated fatty acids in meat were 36.628% and 26.739%, respectively, while the cholesterol reached 79.18 mg/100 g of meat. In conclusion, meat of Bali bull fattened using forage crops in small holder farming has higher saturated fatty acid than unsaturated fatty acids.

HISTOMORPHOMETRY OF SEMINIFEROUS TUBULES OF LOCAL GOATS FIXED WITH DIFFERENT FIXATIVES Fitriani, Fitriani; Wahyuni, Sri; Akmal, Muslim; Dasrul, Dasrul; Iskandar, Cut Dahlia
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i3.11139

The aim of this study was to further understand the histomorphometry of seminiferous tubules from local goat testicles that were fixed with different fixatives. Nine testicles were randomly chosen, each fixed with 10% formalin, 10% neutral buffered formalin (NBF), and 4% paraformaldehyde (PFA) solutions. The sample was then proceeded to histology preparation and stained with haematoxylin eosin (HE). Observation of the tissue was carried out using microscope and the data obtained was analyzed statistically. The results showed that there was a significant difference (P0.05) on each fixative in tubular diameter and membrane, and very significant difference (P0.01) on germinal cell and staining absorbability. Duncan test result on tubular diameter showed that the use of PFA fixative was significantly different (P0.05) compared to formalin fixative while the use of NBF fixative had no significant difference (P0.05) compared to PFA fixative. In addition, the measurement of interstitial membrane of seminiferous tubules showed no significant difference (P0.05) among three fixatives. To conclude, formalin, NBF, and PFA fixatives affect seminiferous tubules, basal membrane structure, and germinal cell appearance inside tubular lumen. Type of tissues and fixative must be considered in selecting the suitable fixative.

IMPROVING EWE OOCYTE VIABILITY AFTER VITRIFICATION WARMING USING COMBINATION OF DIFFERENT CRYOPROTECTANT AND CARRIER SYSTEM Widyastuti, Rini; Setiawan, Rangga; Syamsunarno, Mas Rizky Anggun Adipurna; Ghozali, Mohammad; Saili, Takdir; Boediono, Arief
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i3.11398

The aim of this study was to determine the best combination of cryoprotectant (Ethylene glycol, EG), dimethyl sulfoxide (DMSO) and propanediol (PrOH) and carrier system (hemistraw and cryotop) in improving ewe oocytes viability during cryopreservation. Oocytes with multi layers of compact cumulus cells were colleted from abbatoir and matured in TCM 199 medium supplemented with 10% fetal calf serum for 24-26 h at 38.5 C under 5% CO2 in the air. Matured oocyte was divided into six parts and vitrified in three different vitrification solutions; (i) 17% EG+17% DMSO with hemistraw as carrier system, (ii) 34% EG with hemistraw as carrier system, (iii) 17% EG+17% PrOH in hemistraw (iv), 17% EG+17% DMSO with cryotop as carrier system (v), 34% EG with cryotop as carrier system (vi), and 17% EG+17% PrOH in cryotop. Oocytes were cryopreserved for one week before revived and evaluated for viability. The result showed that oocytes vitrified in media containing EG and DMSO in cryotop had the highest viability (88.16%) compared to media containing EG only or EG and PrOH (70.95% and 68.76%, respectively) (P0.05). Moreover, oocytes viability that vitrified using cryotop and hemistraw as carrier system were not significantly different. The present results indicated that vitrification using combination of EG and DMSO as permeable cryoprotectant and cryotop as carrier system was the best system to maintain oocyte viability after vitrification-warming.

MORPHOLOGY AND MORPHOMETRY OF Haemonchus contortus IN GOATS IN YOGYAKARTA, INDONESIA Sambodo, Priyo; Prastowo, Joko; Indarjulianto, Sudarmanto; Kurniasih, Kurniasih
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i3.11026

This research was carried out to determine the morphology and morphometry of Haemonchus contortus in goats. Adult female parasites were collected from abomasum of naturally infected goats and stored in lactophenol. Images of the parasite were acquired using lucida camera and measurement was performed using Axiovision LE software. Basic characters of the morphology and morphometry of Haemonchus spp. were identified by determining the value of the discriminant function of three parameters on spicule. Vulvar morphology was identified by vulvar flap shape. Data were presented in terms of mean, standard deviation, and percentage. A hundred percent of the parasites were H. contortus with discriminant function value 0.63. The size of female parasites was longer and wider (25.5-32.6 mm; 0.38-0.63 mm) than that of male ones (17.3-20.0 mm; 0.24-0.33 mm). There were only 2 varieties of vulvar flap, linguiform (81%), and knobbed (19%). There were 5 subclasses identified in the linguiform type, including linguiform A (39%), linguiform B (22%), linguiform C (17%), linguiform I (1%), and a new subclass of linguiform D (2%). Based on the the type of vulvar flap, H. contortus found in goats in Yogyakarta were different to those found in previous researches. The findings could be used in identifying the parasite species in small ruminants.

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Tongku Nizwan Siregar

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+626517551536

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jkh@usk.ac.id

Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

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Kab. aceh besar,

Aceh

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Tongku Nizwan Siregar

Contact Email jkh@usk.ac.id

Phone +626517551536

Journal Mail Official jkh@usk.ac.id

Editorial Address Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location Kab. aceh besar, Aceh INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Tongku Nizwan Siregar

Contact Email
jkh@usk.ac.id

Phone
+626517551536

Journal Mail Official
jkh@usk.ac.id

Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA