Jurnal Kedokteran Hewan
Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.
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EVALUATION OF LAPAROTOMY SURGERY WOUND HEALING IN DOGS USING THERMOGRAPHIC ANALYSIS
Agustina Dwi Wijayanti;
Agus Purnomo;
Hery Wijayanto
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala
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DOI: 10.21157/j.ked.hewan.v17i1.29785
This study aimed to develop the use of thermographic analysis to evaluate wound healing for laparotomy surgery in animals. The experiment used nine adult dogs 5 males and 4 females, bodyweight 5-10 kg) that had undergone the same general anesthesia and laparotomy procedures. Evaluation of wound healing was performed at 24, 48, and 72 hours after surgery and 7 days after operations using a digital thermal camera. During each thermographic evaluation period, blood samples were taken for analysis of total leukocytes and leukocyte differential. The results of thermal imaging were compared with the values of inflammatory cells and the clinical condition of wound healing. Comparison of thermographic analysis with inflammatory status was evaluated using the regression equation and showed a strong correlation coefficient (Y= -0.4847 + 40.14, R2= 0.867), that is, the higher the temperature, the lower the inflammation. The conclusion of study is that thermographic analysis can be used to evaluate wound healing of laparotomy surgery in dogs.
VENTRAL MEDIAN AND LATERAL FLANK APPROACH FOR OVARIOHYSTERECTOMY IN CAT
Bella Vera;
Riki Siswandi;
Raden Harry Soehartono
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala
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DOI: 10.21157/j.ked.hewan.v17i1.29418
This study was conducted to compare Ventral Median Approach (OHM) and Lateral Flank Approach (OHF) for feline ovariohysterectomy (OH). Fifteen healthy local female cats (Felis catus) with body weights ranged 2-4 kg and aged 1-2 years were divided into two groups, OHM (n= 8) and OHF (n= 7). Prior to OH, the cats were anesthethized using ketamine-xylazine and the sugery was performed by the same operator. The wound scoring showed a significantly higher difference in the OHM group on day 3 and 4 post-surgery. Scores of erythematous, swelling and dehiscence showed no significant differences. The 4Avet pain scale was significantly higher in OHF at the 2, 6, and 12-hours post surgery. The pain response was evaluated using baseline tactile Semmes-Weinstein Monofilaments and was significantly higher in OHF than OHM on day 1 to day 5 post surgery. Blood hematology, blood glucose and wound healing duration did not differ significantly between groups. The OHF approach showed greater post-operative pain than the OHM approach. Wound dehiscence was observed in two cats (25%) in OHM group from day 4 post-operative. The OHM approach showed a greater risk of complications than the OHF group and therefore requires better post-operative care. The OHF group showed a smaller risk of complications but requires better post-operative analgesic therapy.
HISTOMORPHOLOGY OF PANCREATIC TISSUE IN MEDAKA FISH (Oryzias javanicus): STUDY OF DIABETIC ANIMAL MODEL DEVELOPMENT
Muhammad Ichlasul Akmal;
Sri Rahmatul Laila;
Tutik Wresdiyati
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala
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DOI: 10.21157/j.ked.hewan.v17i1.26884
This study aimed to explore the histomorphology of the pancreatic tissue of medaka fish to develop medaka fish as a diabetic animal model. Adult medaka fish were obtained with a body length of 2.53±0.22 cm and a weight of 265.8±25 g. Medaka fish were fixed using Boiun's fixative solution. Macro anatomical observations of the pancreas were conducted using a stereomicroscope. Histological preparations were made according to the Hematoxylin-eosin staining standard procedure. Pancreatic histomorphology of medaka fish was carried out using a light microscope with 100x and 400x magnification. The macro anatomy observations of the pancreas showed the interconnection with several tissues, such as spleen, gallbladder, liver, fat, and intestines. The pancreas was located toward the liver, oversees the intestines, and covered with fat. Pancreatic histomorphological observations showed exocrine cells composed of serous pyramidal acinar cells and endocrine cells (islets of Langerhans). Intralobular and intercalated ducts (ducts of Boll) were composed of simple cuboidal epithelium. This study concluded that the pancreas organ in medaka fish is a single organ, and histomorphologically has both of exocrine and endocrine cells.
CAPABILITY OF DIFFERENT BREEDS OF DONOR CATTLE TO PRODUCE EMBRYOS AT THE CIPELANG LIVESTOCK EMBRYO CENTER, BOGOR, WEST JAVA
Crista Damaris;
Anny Rosmayanti;
Siti Darojah;
Nurul Isnaini
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala
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DOI: 10.21157/j.ked.hewan.v17i1.24673
The objective of this research was to investigate the capability of the donor cattle to produce embryos by comparing three cattle breeds, namely Bos taurus beef cattle (Limousin), Bos taurus dairy cattle (Friesian Holstein/FH), and Bos indicus beef cattle (Peranakan Ongole/PO). This research was observational method with in vivo embryo production method and a nonsurgical embryo collection method (flushing). The data collected were then processed using the one way ANOVA method. The results showed that the number of CL, response rate, total number of embryos, and recovery rate in PO cattle, FH cattle, and Limousin cattle did not differ significantly (P0.05). The appropriate number of embryos to be transferred and degenerative embryo were significantly different among PO cattle, FH cattle, and Limousin cattle (P0.05), while the unfertil ova, fertilization rate, and transferable embryo did not differ among cattle breed (P0.05). This research concluded that each breed of the donor cattle produced embryos with varies quality and the best quality of embryo production is found in Limousin cattle.
THE APPLICATION OF YEAST AND LACTIC ACID BACTERIA IMPROVE GROWTH AND INTESTINAL STRUCTURE OF TIGER SHRIMP LARVAE Penaeus monodon Fab.
Muhammadar Abdullah Abbas;
Nadhira Alamanda;
Ibnu Sahidir;
Adrian Damora;
Cut Nanda Devira;
Dedi Fazriansyah Putra
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala
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DOI: 10.21157/j.ked.hewan.v17i1.30379
This study aims to investigate the effect of the different concentration of RABAL (yeast and lactic acid bacteria) probiotics on the survival, feed conversion, growth and intestinal cells changes of tiger shrimp Penaeus monodon Fab. larvae. This study used a Completely Randomized Design (CRD) method with 4 treatments and 4 replications. Tiger shrimp with average size of 0.02 g was randomly stocked in each container containing RABAL with concentration of 0 ppm (A/control), 75 ppm (B), 150 ppm (C), and 225 ppm (D) with frequency once a day for 30 days. Data were analysed using one way ANOVA. The result showed that the application of RABAL with different doses into the shrimp culture media significantly influences (P0.05) the growth and food conversion ratio of tiger shrimp feed but did not significantly affect (P 0.05) on the survival rate. The value of water quality parameters in this study were still at normal levels for tiger shrimp rearing. As conclusion, the application of probiotics RABAL with different concentrations have positive effect on the absolute weight, absolute length, specific growth rate, feed conversion ratio and intestinal villi surface area of tiger shrimp larvae, without adverse effect on the survival rate of tiger shrimp seed.
EFFECT OF ETHANOLIC NEEM (Azadirachta indica) LEAVES EXTRACT ON DEVELOPMENT OF SPERMATID AND ANDROGEN RECEPTOR EXPRESSION IN THE TESTIS OF RABBIT
Usma Aulia;
Sri Wahyuni;
Gholib Gholib;
Dasrul Dasrul;
Mulyadi Adam;
Erdiansyah Rahmi;
Abdullah Hamzah
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala
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DOI: 10.21157/j.ked.hewan.v17i1.30042
The objective of this study was to determine the effect of ethanol extract of neem (Azadirachta indica) leaves on the number of spermatids and androgen receptor expression in the testicular tissue of New Zealand White (NZW) rabbits. Twelve adults male NZW rabbits with body weight (BW) 2-2.5 kg were divided into three treatment groups (n = 4), namely P1 (control group) given 0 mg/kg BW, P2 and P3 were given 200 mg/kg BW and 300 mg/kg BW of neem leaves ethanol extract respectively. The extract was administered orally using a gastric tube for 52 days. At the end of the treatment, the rabbits were terminated and the testes were collected to be processed into histological preparations and stained with Periodic Acid Schiff (PAS) staining to detect the number of spermatid and Immunohistochemical (IHC) staining to detect androgen receptor expression. The results showed that neem leaves extract administered at P3 rabbits significantly decreased the number of spermatids and androgen receptor expression in myoid peritubular cells and connective tissue P0.05), but not significantly different in the Leydig cells and blood vessels (P0.05) of testicular tissue. The conclusion of this study is that the ethanol extract of neem leaves at a dose of 300 mg/kg BW can be used as a contraceptive candidate compared to a dose of 200 mg/kg BW.
ROBUST PREDICTION OF MEAT QUALITY ATTRIBUTES USING NEAR INFRARED SPECTROSCOPY
Cut Dahlia Iskandar;
Z Zainuddin;
Agus Arip Munawar;
Rina Aulia Barus
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala
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DOI: 10.21157/j.ked.hewan.v17i1.30204
The main purpose of this present study is to evaluate the ability of near infrared technology as an alternative method in determining and assessing quality parameters of meat product where in this case is frozen beef. At first, beef samples from chest and legs parts were sliced and taken at the amount of 100g per sample to be frozen. Then spectra data of beef samples were obtained using near infrared spectrophotometer (PSD IR i16) in wavelength range from 1000 to 2500 nm with optical gain 4x. Actual protein contents were obtained by Kjeldahl method and measured in triplicate. The near infrared spectra data were enhanced and improved by means of mean centering (MC) and baseline shift correction (BSC) methods. The results showed that protein content of frozen beef samples can be predicted rapidly with maximum correlation coefficient is 0.91. Heat properties of beef samples changes exponentially during freezing and thus, optimum freezing temperature and time can be predicted as well. Based on those obtained results, it may conclude that near infrared technology can assess frozen beef qualities rapidly and effectively.
HISTOMORPHOLOGY OF PANCREATIC TISSUE IN MEDAKA FISH (Oryzias javanicus): STUDY OF DIABETIC ANIMAL MODEL DEVELOPMENT
Akmal, Muhammad Ichlasul;
Laila, Sri Rahmatul;
Wresdiyati, Tutik
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala
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DOI: 10.21157/j.ked.hewan.v17i1.26884
This study aimed to explore the histomorphology of the pancreatic tissue of medaka fish to develop medaka fish as a diabetic animal model. Adult medaka fish were obtained with a body length of 2.530.22 cm and a weight of 265.825 g. Medaka fish were fixed using Boiun's fixative solution. Macro anatomical observations of the pancreas were conducted using a stereomicroscope. Histological preparations were made according to the Hematoxylin-eosin staining standard procedure. Pancreatic histomorphology of medaka fish was carried out using a light microscope with 100x and 400x magnification. The macro anatomy observations of the pancreas showed the interconnection with several tissues, such as spleen, gallbladder, liver, fat, and intestines. The pancreas was located toward the liver, oversees the intestines, and covered with fat. Pancreatic histomorphological observations showed exocrine cells composed of serous pyramidal acinar cells and endocrine cells (islets of Langerhans). Intralobular and intercalated ducts (ducts of Boll) were composed of simple cuboidal epithelium. This study concluded that the pancreas organ in medaka fish is a single organ, and histomorphologically has both of exocrine and endocrine cells.
CAPABILITY OF DIFFERENT BREEDS OF DONOR CATTLE TO PRODUCE EMBRYOS AT THE CIPELANG LIVESTOCK EMBRYO CENTER, BOGOR, WEST JAVA
Damaris, Crista;
Rosmayanti, Anny;
Darojah, Siti;
Isnaini, Nurul
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala
Show Abstract
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DOI: 10.21157/j.ked.hewan.v17i1.24673
The objective of this research was to investigate the capability of the donor cattle to produce embryos by comparing three cattle breeds, namely Bos taurus beef cattle (Limousin), Bos taurus dairy cattle (Friesian Holstein/FH), and Bos indicus beef cattle (Peranakan Ongole/PO). This research was observational method with in vivo embryo production method and a nonsurgical embryo collection method (flushing). The data collected were then processed using the one way ANOVA method. The results showed that the number of CL, response rate, total number of embryos, and recovery rate in PO cattle, FH cattle, and Limousin cattle did not differ significantly (P0.05). The appropriate number of embryos to be transferred and degenerative embryo were significantly different among PO cattle, FH cattle, and Limousin cattle (P0.05), while the unfertil ova, fertilization rate, and transferable embryo did not differ among cattle breed (P0.05). This research concluded that each breed of the donor cattle produced embryos with varies quality and the best quality of embryo production is found in Limousin cattle.
THE APPLICATION OF YEAST AND LACTIC ACID BACTERIA IMPROVE GROWTH AND INTESTINAL STRUCTURE OF TIGER SHRIMP LARVAE Penaeus monodon Fab.
Abbas, Muhammadar Abdullah;
Alamanda, Nadhira;
Sahidir, Ibnu;
Damora, Adrian;
Devira, Cut Nanda;
Putra, Dedi Fazriansyah
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala
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DOI: 10.21157/j.ked.hewan.v17i1.30379
This study aims to investigate the effect of the different concentration of RABAL (yeast and lactic acid bacteria) probiotics on the survival, feed conversion, growth and intestinal cells changes of tiger shrimp Penaeus monodon Fab. larvae. This study used a Completely Randomized Design (CRD) method with 4 treatments and 4 replications. Tiger shrimp with average size of 0.02 g was randomly stocked in each container containing RABAL with concentration of 0 ppm (A/control), 75 ppm (B), 150 ppm (C), and 225 ppm (D) with frequency once a day for 30 days. Data were analysed using one way ANOVA. The result showed that the application of RABAL with different doses into the shrimp culture media significantly influences (P0.05) the growth and food conversion ratio of tiger shrimp feed but did not significantly affect (P 0.05) on the survival rate. The value of water quality parameters in this study were still at normal levels for tiger shrimp rearing. As conclusion, the application of probiotics RABAL with different concentrations have positive effect on the absolute weight, absolute length, specific growth rate, feed conversion ratio and intestinal villi surface area of tiger shrimp larvae, without adverse effect on the survival rate of tiger shrimp seed.