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Mukhtabar: Journal of Medical Laboratory Technology
Published by Sekolah Tinggi Ilmu Kesehatan Muhammadiyah Ciamis
ISSN : -     EISSN : 29870224     DOI : https://doi.org/10.52221/mjmlt.v1i1
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology
Mukhtabar Journal of Medical Laboratory Technology (MJMLT) is a peer-reviewed journal published by the Research and Community Service Board of STIKes Muhammadiyah Ciamis since 2023. MJMLT is registered with the International Standard Serial Number (ISSN) with e-ISSN: 2987-0224. This journal is also registered in the CrossRef system with DOI 10.52221/mjmlt. MJMLT is currently indexed in DOAJ (Directory of Open Access Journals). MJMLT is published twice a year (April and October). MJMLT is a peer-reviewed scientific journal covering publications containing research on laboratories in the medical field and related topics. All activities that have a relationship with technology (both technology application and development) can be published in this journal. Scientific articles addressing the topics of immunoserology, parasitology, microbiology, clinical chemistry, haematology, toxicology, molecular biology, and disease detection are particularly welcome. Articles of both professional and scientific interest will be considered. The journal accepts manuscripts in the following categories: Original articles Review articles (Systematic Literature Review and Meta-Analysis) Research letter (short communication) Case study Editorial Bibliometric Analysis (Related to Medical Technology) as well as several supplements each year. Supplements consist of monographs, as well as symposium and congress reports covering subjects in the medical laboratory science discipline and related topics.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Bioteknologi
Articles 36 Documents
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Effect of Variation Natrium Sitrat Concentration and Centrifugation Time on Prothrombin Time (PT) Value Najiyyah, Amalia Nur; Marliana, Nina; Hayati, Eem; Rahmat, Mamat
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 2 (2024): Mukhtabar: Journal of Medical Laboratory Technology (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i2.694

Abstract

Background & Objective: Prothrombin Time (PT) test is one of the hemostasis tests used to determine whether there is a deficiency of extrinsic clotting factors and co-pathway clotting factors, namely factors I, II, V, VII, and X. The pre-analytical stage that must be considered in the hemostasis examination is the concentration of anticoagulant used and the centrifugation process. This study was conducted to determine the effect of variations in sodium citrate anticoagulant concentration and centrifugation time on Prothrombin Time (PT) values.  Method: The anticoagulants used in this study were 3.2% and 3.8% sodium citrate. Centrifugation is the process of separating cells from plasma. The effect of improper centrifugation is to increase the number of platelets, so that platelet factors contained in platelets will accelerate the formation of fibrin. The research conducted is a pseudo-experimental study by giving treatment to blood specimens that will be added with anticoagulant Sodium Citrate concentrations of 3.2% and 3.8% with a blood ratio of 9:1, then centrifuged at 3000 rpm and given a variation of time for 15 minutes, 10 minutes, and 5 minutes. The results were tested using Saphiro Wilk and Friedman Test. Result : Based on the research that has been done, the Asymp. Sig. value obtained in the Friedman Test is >0.05. This indicates that the treatment that has been given to the sample has no effect on the value of Prothrombin Time. Conclusion: It can be concluded that there is no significant difference between the variation of sodium citrate concentrations of 3.2% and 3.8%, as well as in the variation of 3000 rpm centrifugation time of 15 minutes, 10 minutes, and 5 minutes on the value of Prothrombin Time (PT).
Effectiveness of Lime Concentration As A Modification of Turk's Reagent in Leucocyte Type Count Examination Arham, Chaerul; Rasyid, Nur Qadri; Rianto, Rifo; Moito, Rahma
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 2 (2024): Mukhtabar: Journal of Medical Laboratory Technology (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i2.695

Abstract

Background & Objective: Turk solution has a composition of glacial acetic acid, gentian violet, and distilled water. Lime (Citrus aurantifolia S) has a pH of 2.0 which is almost the same as acetic acid. Both components are weak acids that can lyse blood cells other than white blood cells. This study aims to determine the effectiveness of lime concentration as a modification of Turk's reagent in counting the number of leukocytes and knowing the concentration of 1%, 2%, or 3% which is more effective in counting the number of leukocytes. Method: This study aims to provide an overview of the effectiveness of lime concentration as a modification of turk reagent composition in counting the number of leukocytes. The research method used is the Counting Room Method. This research was conducted in August 2024 at the clinical pathology laboratory of the Muhammadiyah Makassar Polytechnic. The number of samples in this study was 4 blood samples obtained from 4 correspondents with treatment, lime concentrations namely 1%, 2%, and 3%, and turk reagent as control. Result: At 1% concentration, the average difference in leucocyte count between the control and the sample was 1,150 cells/mm3, at 2% concentration a difference of 2,788 cells/mm3 was obtained with the control, and at 3% concentration a difference of 3,638 cells/mm3 was obtained. Conclusion: It can be concluded that the concentration of lime that is effective as a modification of Turk's reagent in counting the number of leukocytes is a concentration of 1%.
Optimization of Concentration and Staining Duration of Methyl Green in The Examination of Escherichia coli DNA Bands Using Agarose Gel Electrophoresis Adrian Prasetya, Rifky; Merdekawati, Fusvita; Iin Nur Indra, Asep; Djuminar, Ai
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 2 (2024): Mukhtabar: Journal of Medical Laboratory Technology (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i2.696

Abstract

Background & Objective: Nowadays, many alternative dyes are used for staining DNA during electrophoresis, one of which is Methyl Green. Methyl Green has several advantages, including being cheaper than EtBr, having low toxicity, being non-carcinogenic, and as a cationic dye, Methyl Green is resistant to photobleaching. This study aims to determine the optimal concentration and staining duration of Methyl Green as a DNA dye for examining Escherichia coli DNA bands using agarose gel electrophoresis. Method: The research method used is experimental. Amplified Escherichia coli 16S rRNA gene DNA, sized 584 bp, which has undergone electrophoresis, was stained with Methyl Green dye at concentrations of 0.10%, 0.15%, 0.20%, 0.25%, 0.00015%, 0.00020%, 0.00025%, and 0.00030% with varying immersion times of 10, 15, 20, and 25 minutes. Result: The resulting DNA bands were analyzed or measured for surface area using ImageJ software. The mean value for each experimental group was calculated. The highest mean value was used as the basis for determining the most optimal condition. Conclusion: This study concludes that the optimal concentration and staining duration of Methyl Green, based on the highest mean value of 19,844,845, is 0.00030% Methyl Green with a staining duration of 25 minutes.
Identification of Malassezia sp. as a Cause of Dandruff Among Students at Muhammadiyah Polytechnic Makassar Annisa, Riska; Rasyid, Nur Qadri; Basarang, Mujahidah; Faisal, Restu Andini
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 2 (2024): Mukhtabar: Journal of Medical Laboratory Technology (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i2.703

Abstract

Background & Objective: Dandruff is a scalp condition attributed to the fungus Malassezia sp. It manifests as fine to coarse scales that are grayish or white, resulting from the shedding of the stratum corneum layer on the scalp. Factors contributing to the onset of dandruff include the proliferation of Malassezia fungi, sebaceous gland activity, and individual predisposition. This study aimed to identify Malassezia sp. responsible for dandruff among female students at the Muhammadiyah Polytechnic of Makassar. Method: The research employed a laboratory observational design utilizing a purposive sampling technique. Dandruff samples were collected from 10 female students and cultured on SDA (Sabouraud Dextrose Agar) media, followed by microscopic examination of the resulting colonies. Result: The findings revealed that none of the 10 dandruff samples contained Malassezia sp.; instead, contamination by Aspergillus fumigatus was observed. Conclusion: Therefore, it can be concluded that Malassezia sp. was not identified as a causative agent of dandruff in female students at the Muhammadiyah Polytechnic of Makassar
Identification of Soil Egg Transmitted Helminth on The Nails of Cleaning Workers At TPS Wonocolo District Surabaya City Damayanti, Chiara Shabrina; Artanti, Dita; Ariana, Diah; Samsudin, Rinza Rahmawati
Mukhtabar Journal of Medical Laboratory Technology Vol 3 No 1 (2025): Mukhtabar: Journal of Medical Laboratory Technology (April 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v3i1.706

Abstract

Background & Objective:  In Indonesia, worm infections are still a big problem in public health because the prevalence is still high, namely 45-65%. even in certain areas that have poor environmental sanitation, high heat, and humidity, the prevalence of worm infections can reach 80%. Worm infections can cause a decline in the health condition, nutrition, and productivity of sufferers, resulting in economic losses, due to a decrease in carbohydrates, protein, and blood, which has an impact on reducing the quality of human resources, one of which is transmission through the nails. This study aims to determine whether or not there are Soil Transmitted Helminth (STH) worm eggs in the nails of cleaning workers in Wonocolo District, Surabaya City. Method: The research method used was deposition (sedimentation) centrifugation using 0.25% NaOH solution, which was analyzed descriptively. The sampling technique used was purposive sampling by taking nail samples from 30 waste cleaning officer respondents. Result: The results of the research showed that 17% of the fingernails of waste cleaners were contaminated with Soil Transmitted Helminth (STH) worm eggs, while 83% of nail samples that were not contaminated with Soil Transmitted Helminth (STH) worm eggs were contaminated. Conclusion: Based on the research results, it can be concluded that there are still cleaning workers in Wonocolo District, Surabaya City, who are contaminated with Soil Transmitted Helminth (STH) worm eggs. This happens because their awareness about personal hygiene, sanitation, Clean and Healthy Living Behavior (CHLS), and the use of Personal Protective Equipment (PPE) while working is still lacking.
Overview of Lymphocyte Count Examination in Patients with Advanced Phase Pulmonary Tuberculosis at Ciamis Regional General Hospital Istianah, Euis Tia; Firdaus, Selvira Rahma; Wilujeng, Endang Octaviana
Mukhtabar Journal of Medical Laboratory Technology Vol 3 No 1 (2025): Mukhtabar: Journal of Medical Laboratory Technology (April 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v3i1.786

Abstract

Background & Objectives: Pulmonary tuberculosis is a contagious infectious disease caused by Mycobacterium tuberculosis. This infection can be cured through two phases of treatment, namely the intensive phase and the continuation phase. The intensive phase is carried out for two months while the continuation phase is carried out for four months. Tuberculosis treatment is carried out by administering Anti-Tuberculosis Drugs (OAT) consisting of Rifampicin, Ethambutol, Isoniazid, and Pyrazinamide. To monitor the success of treatment and the healing process of tuberculosis, a hematology examination can be done, namely a lymphocyte cell count. In a body infected with bacteria, lymphocyte cells will decrease, after treatment is given, lymphocyte cells will increase or return to normal. The purpose of this study was to see the picture of the results of the lymphocyte count examination in patients with advanced phase pulmonary tuberculosis.Method: This research method is descriptive which was conducted on patients with advanced phase pulmonary tuberculosis at the Ciamis Regional General Hospital in May 2024 with a sample size of 30 respondents. The study used secondary data and primary data which were processed into tables and then explained in narrative. The research was conducted using a hematology analyzer. The research was conducted in May 2024 at the hematology laboratory of the Ciamis Regional General Hospital.Results: From this study obtained the results of 2 (7%) samples with lymphopenia results, as many as 25 (83%) samples with normal lymphocyte results and 3 (10%) samples with lymphocytosis results.Conclusion: The results of the lymphocyte count examination in patients with advanced phase pulmonary tuberculosis found an average normal lymphocyte count.
Formulation and Evaluation of Clove Flower Extract (Syzygium aromaticum L.) Toothpaste Aidie With Variations of NA CMC Concentration as a Binder Herawayanti, Sinta; Indriastuti, Marlina
Mukhtabar Journal of Medical Laboratory Technology Vol 3 No 1 (2025): Mukhtabar: Journal of Medical Laboratory Technology (April 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v3i1.790

Abstract

Background & Objective: Toothpaste is a semi-solid preparation that contains abrasives, cleansers, and additives to clean teeth without damaging oral tissues. Clove flower (Syzygium aromaticum L.) is known to contain eugenol, saponins, and flavonoids, which are analgesic. The purpose of this study was to make and evaluate toothpaste preparations from clove flower extract. Method: The research was conducted experimentally with toothpaste formulation using variations in Na CMC concentration as a binder: 2%, 1%, and 0.5%. Evaluation included organoleptic test, homogeneity, pH, viscosity, spreadability, foam formation, and physical stability. Data were analyzed using SPSS. Result: All formulas met the evaluation standards. Formula I was closest to the positive control in organoleptic, having an average viscosity of 9600 mPa.s and spreadability of 5.2 cm. Formula II showed the best pH with an average of 8.74. All three formulas produced foam well. Physical stability for 4 weeks showed that the texture and pH remained stable. Conclusion: Na CMC affects the viscosity and spreadability of toothpaste preparations. Formulas with the right concentration of Na CMC can produce herbal toothpaste that is stable and meets quality requirements.
Description of Hemoglobin Levels in Patients With Pulmonary Tuberculosis in The Intensive Treatment Stage at RSUD Ciamis Wilujeng, Endang Octaviana; Dewi, Ayu Ratna; Istianah, Euis Tia
Mukhtabar Journal of Medical Laboratory Technology Vol 3 No 1 (2025): Mukhtabar: Journal of Medical Laboratory Technology (April 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v3i1.792

Abstract

Background & Objectives: Tuberculosis is one of the leading causes of death in the world. Pulmonary tuberculosis is a chronic disease that infects the lungs caused by Mycobacterium tuberculosis and can affect hemoglobin levels to become anemic. Anemia occurs due to insufficient iron intake and the use of anti-tuberculosis drugs (OAT). Anemia is defined as reduced hemoglobin levels in the blood. Hemoglobin is a protein that contains iron in red blood cells. This study aims to determine the description of hemoglobin levels in patients with pulmonary tuberculosis in the intensive treatment stage at Ciamis Regional Hospital.Methods: This research method is descriptive. Data collection techniques were obtained from secondary data. Sampling was done by purposive sampling technique which recruited 31 patients with pulmonary tuberculosis to be used as respondents. Results: From this study, 19 (61%) samples with normal hemoglobin levels and 12 (39%) samples with low hemoglobin levels were obtained.Conclusion: Based on the results of the examination of hemoglobin levels in patients with pulmonary tuberculosis in the intensive treatment stage at Ciamis Hospital, most of them have normal hemoglobin levels.
Assessment of Analytical Specificity in qPCR: SYBR Green and TaqMan Probe Methods for Isonazid-Resistant Mycobacterium tuberculosis Merdekawati, Fusvita; Suryawan, Muhammad Raihan; Nurhayati, Betty; Rinaldi, Sonny Faisal; Juliastuti, Aditya
Mukhtabar Journal of Medical Laboratory Technology Vol 3 No 1 (2025): Mukhtabar: Journal of Medical Laboratory Technology (April 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v3i1.793

Abstract

Background & Objective: Mycobacterium tuberculosis, often referred to as M. tuberculosis, is an infectious pathogen that is responsible for causing tuberculosis (TB), a prevalent condition that is a leading cause of death globally. The spread of tuberculosis bacteria that are resistant to certain medicines, such as isoniazid, is currently on the rise. Therefore, molecular testing, such as quantitative polymerase chain reaction (qPCR), is needed to rapidly and reliably identify isoniazid-resistant tuberculosis germs. Method: The purpose of this study was to assess the analytical specificity of TaqMan Probe and SYBR Green qPCR techniques for the detection of isoniazid-resistant tuberculosis. This study was descriptive and quantitative. Analytical specificity was ascertained using MTB DNA with the S315G mutation spiked with E. coli DNA. A paired t-test was used to assess the primary data. Result: The results show that the analytical specificity values based on the significance of the paired t-test for the SYBR Green and TaqMan Probe methods were 0.398 and 0.790, respectively. Conclusion: Based on these results, it can be concluded that the analytical specificity of the TaqMan Probe qPCR method was 1.99 times greater than that of the SYBR Green method.
Evaluation of Quality Indicators with Lean Six Sigma In Pre and Post Analytical Laboratories Nurhadi, Muhammad Ihsan; Ridwanna, Surya; Rinaldi, Sonny Feisal; Nurhayati, Betty
Mukhtabar Journal of Medical Laboratory Technology Vol 3 No 1 (2025): Mukhtabar: Journal of Medical Laboratory Technology (April 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v3i1.795

Abstract

Background & Objective: Medical laboratories have an important role of 60-70% in diagnosis, patient monitoring, and prevention and treatment of diseases. Therefore, the laboratory must maintain and ensure the quality of service process. This study aims to determine the sigma value and identify waste in pre and post analytical in the laboratory, as well as determine Improvement proposals in order to reduce errors pre and post analytical laboratory. Method: This research design is descriptive study that Analyzes the process and quality indicators at pre and post analytical with the Lean Six Sigma approach, which is a combination of Lean methods that focus on eliminating waste and Six Sigma that focuses on eliminating defects. This research was conducted as a process Improvement effort with five Six Sigma work steps (DMAIC) and identified eight types of waste (DOWNTIME). Result: The results showed that the sigma value at pre and post analytical was 4.6 and 3.5 Sigma, and total sigma value for pre-post analytical was 4.3 Sigma so that both had not met the minimum target achievement of 5 Sigma (Excellent). There are two quality indicators that require improvement and enhancement including suitability of sample and TAT. The results also show that there are 4 wastes, consisting of 1 Defects, 1 Waiting, and 2 Not Utilizing Employees Knowledge. Conclusion: Proposed Improvements are then given so that all waste identified in this study can be minimized so that the achievement of sigma quality indicators can increase.

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