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Jurnal Laboratorium Khatulistiwa
Published by Poltekkes Kemenkes Pontianak
ISSN : 25979523     EISSN : 25979531     DOI : https://doi.org/10.30602/jlk
Core Subject : Health, Science, Education,
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of Medical Laboratory. Jurnal Laboratorium Khatulistiwa particularly focuses on the main problems in the development of the Medical Laboratory health areas as follows: Toxicology Immunoserology Bacteriology Clinical Chemistry Parasitologi Micology And other related disciplines.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Imunologi dan Mikrobiologi (Lain-Lain)
Articles 171 Documents
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Evaluation of Rice Bran as an Alternative Growth Medium for Rhizopus sp. Dina Aribah; Mazidah Nabila Ridwan
Jurnal Laboratorium Khatulistiwa Vol 9, No 2 (2026): MEI 2026
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i2.2274

Abstract

Rice bran is an abundant agroindustrial by-product with high nutritional content, yet its utilization in microbiology remains limited. Meanwhile, Rhizopus sp., widely used in laboratory research, requires a sustainable and cost-effective culture medium. This study aimed to evaluate the potential of rice bran as an alternative growth medium for Rhizopus sp. This study used rice bran media at concentrations of 5%, 10%, and 15% (w/v), with Sabouraud Dextrose Agar (SDA) as a control. Inoculation was performed using the single dot method. Mold growth was observed after 72 hours of incubation at ±30°C, both macroscopically and microscopically. Colony diameters were measured and analyzed using one-way ANOVA. The results showed that Rhizopus sp. grew well on all rice bran media, displaying complete vegetative and reproductive structures. Statistical analysis indicated no significant difference in colony diameter between rice bran media and SDA (p>0.05). These findings demonstrate that rice bran can serve as a cost-effective and sustainable alternative medium without compromising fungal growth quality.
Antibakteri Deodorant Spray Minyak Atsiri Bunga Cengkeh terhadap Staphylococcus aureus Dhifa Wira Wicaksana; Edy Suwandi; Etiek Nurhayati; Sugito Sugito; Imma Fatayati; Emilda Sari
Jurnal Laboratorium Khatulistiwa Vol 9, No 2 (2026): MEI 2026
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i2.2127

Abstract

Body odor is a common problem that can affect personal comfort and social confidence. Understanding the relationship between clove flower essential oil concentration and the inhibition of Staphylococcus aureus growth is important for improving the effectiveness of natural deodorant formulations. This study aimed to analyze the relationship between the concentration of clove flower essential oil deodorant spray and the inhibition of Staphylococcus aureus among samples tested at the laboratory. This study used an experimental design with a total sample of 30 samples. Data were collected using the bacterial culture diffusion method on Mueller Hinton Agar (MHA) media and analyzed using the simple linear regression test with a significance level of p < 0.05. The results showed that 15% of samples demonstrated antibacterial activity. Statistical analysis revealed a significant relationship between clove flower essential oil deodorant spray concentration and inhibition of Staphylococcus aureus growth (p = 0.000). The concentration of clove flower essential oil deodorant spray significantly influences the inhibition of Staphylococcus aureus. Early development and utilization of natural-based deodorant formulations are recommended to improve product effectiveness and safety.
Comparison of Diff-Quick and Papanicolaou Staining Quality in Exudative Pleural Effusion Cytology at Buleleng Regional General Hospital Bagus Yoga Prawira; Yeni Avidhatul Husnah
Jurnal Laboratorium Khatulistiwa Vol 9, No 2 (2026): MEI 2026
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i2.2267

Abstract

Exudative pleural effusion cytology is an important diagnostic supporting examination used to evaluate cellular abnormalities in the pleural cavity. The quality of cytological staining plays a critical role in ensuring accurate interpretation of cellular morphology and pathological changes. This study aimed to compare the staining quality between Diff-Quick and Papanicolaou methods in exudative pleural effusion cytology at Buleleng District General Hospital. This study employed a descriptive analytic design with a comparative approach. The samples consisted of 32 exudative pleural effusion cytology slides stained using Diff-Quick and Papanicolaou methods. Staining quality assessment was based on four parameters, including cellular morphology, cytoplasmic staining intensity, nuclear staining intensity, and background clarity. Data were analyzed using the Wilcoxon signed-rank test. The results demonstrated a statistically significant difference between Diff-Quick and Papanicolaou staining methods (p = 0.004; p ≤ 0.05). Papanicolaou staining showed superior visualization of cellular morphology, nuclear detail, and background clarity, whereas Diff-Quick staining provided relatively satisfactory cytoplasmic staining intensity. Overall, the Papanicolaou staining method produced better staining quality compared to the Diff-Quick method in exudative pleural effusion cytology. These findings indicate that Papanicolaou staining remains more effective for detailed cytomorphological evaluation in pleural effusion specimens.
Antibacterial Effect of Caesalpinia sappan L. Aqueous Decoction on Staphylococcus aureus Sri Tumpuk; laila kamilla; Sutriswanto Sutriswanto; Alya Dwinanda Kusumastuti
Jurnal Laboratorium Khatulistiwa Vol 9, No 2 (2026): MEI 2026
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i2.2295

Abstract

Secang (Caesalpinia sappan L.) is a plant frequently utilized in herbal medicine due to its therapeutic properties. Sappan wood contains various bioactive compounds, such as flavonoids, saponins, and tannins, which can inhibit the growth of Staphylococcus aureus. Staphylococcus aureus is a bacterium responsible for conditions such as pressure sores, abscesses, impetigo, cellulitis, and staphylococcal scalded skin syndrome. The aim of this study was to determine the differences in antibacterial activity of sappan wood decoctions at concentrations of 70%, 80%, 90%, and 100% in inhibiting the growth of Staphylococcus aureus. This study employed a quasi-experimental method with a purposive sampling technique. The samples consisted of 70%, 80%, 90%, and 100% concentrations of sappan wood decoction, each repeated six times for a total of 24 samples. Antibacterial testing was conducted using the Kirby-Bauer diffusion method. The results showed that 100% of the tested sappan wood decoction concentrations successfully inhibited the growth of Staphylococcus aureus, with the average inhibition zones being 25 mm at a 70% concentration, 27.16 mm at 80%, 28.58 mm at 90%, and 30.58 mm at 100%. There was a significant relationship between the concentration of sappan wood decoction and its antibacterial activity (p = 0.000). Based on these results, even the lowest concentration of 70% is highly effective for inhibiting the growth of Staphylococcus aureus.
Comparison of Glucose, AST, and Cholesterol Test Results Using Plain Vacutainer Tubes and Clot Activator Vacutainer Tubes Kholidah Zafirah Inas Fatin; Edy Haryanto; Ayu Puspitasari
Jurnal Laboratorium Khatulistiwa Vol 9, No 2 (2026): MEI 2026
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i2.2083

Abstract

Laboratory examinations play a crucial role in supporting disease diagnosis; therefore, laboratory test results must be accurate, valid, and reliable. However, errors may occur during various stages of the examination process, including the selection of blood collection tubes, which can influence test outcomes. In clinical chemistry testing, red-capped vacutainer tubes are commonly used, either plain tubes or tubes containing clot activators. The addition of clot activators should not affect the quality of analyte measurements. This study aimed to compare glucose, AST, and cholesterol levels in serum samples collected using plain vacutainer tubes and vacutainer tubes with clot activators. Serum samples were obtained from 16 respondents and analyzed at a primary healthcare laboratory in Surabaya. This study employed an analytic observational method with a cross-sectional and comparative study design. Data normality was assessed using the Shapiro–Wilk test, followed by the Independent t-test for normally distributed data and the Mann–Whitney test for non-normally distributed data. The results showed no significant differences (p > 0.05) in glucose, AST, and cholesterol levels between serum collected in plain vacutainer tubes and serum collected in vacutainer tubes with clot activators. Therefore, both types of vacutainer tubes can be used interchangeably for these clinical chemistry examinations.
C-Reactive Protein Levels among Obese Students at John Paul II Health Academy Pekanbaru: A Cross-Sectional Study AISYARA YULIANDARI; Irma Br Simanjuntak; Siska Zafrida; Hartini H; Fauzan Fauzan
Jurnal Laboratorium Khatulistiwa Vol 9, No 2 (2026): MEI 2026
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i2.2265

Abstract

Obesity is associated with chronic low-grade inflammation characterized by increased secretion of pro-inflammatory cytokines from adipose tissue, which may stimulate hepatic synthesis of C-Reactive Protein (CRP). Elevated CRP levels are widely recognized as biomarkers of systemic inflammation and may indicate increased risk for metabolic and cardiovascular disorders. This study aimed to evaluate CRP levels among obese students at Akademi Kesehatan John Paul II Pekanbaru. This study employed a descriptive cross-sectional design involving obese students with Body Mass Index (BMI) ≥27 kg/m² selected using purposive sampling. Serum samples were collected and analyzed for CRP levels using qualitative latex agglutination and semi-quantitative methods. A total of 19 serum samples met the inclusion criteria and were included in the analysis. The results showed that 12 respondents (63.2%) had normal CRP levels (
Comparison of Turnaround Time for Complete Blood Count Before and After LIS–HMIS Integration in a Clinical Laboratory I Gede Widiaastawa; Putu Ayu Parwati; Ni Luh Gede Puspita Yanti
Jurnal Laboratorium Khatulistiwa Vol 9, No 2 (2026): MEI 2026
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i2.2269

Abstract

Turnaround Time (TAT) is a key indicator of clinical laboratory performance as it affects diagnostic accuracy and clinical decision-making. This study aimed to compare complete blood count (CBC) TAT before and after the implementation of LIS–HMIS integration at Tabanan Regional General Hospital. This study used a quantitative comparative design with secondary data obtained from LIS and HMIS logs. A total of 316 records before integration (January–March 2025) and 316 records after integration (May–July 2025) were selected using simple random sampling. Data were analyzed using the Mann–Whitney test with a significance level of 0.05. The results showed that the proportion of TAT meeting the standard increased from 71.52% before integration to 75.95% after integration. However, statistical analysis indicated no significant difference (p=0.259). These findings suggest that LIS–HMIS integration improves operational efficiency, although the improvement is not statistically significant.
Association between Working Duration and Hemoglobin Levels among Parking Attendants at Gondanglegi Market, Malang Regency Aina Salma Hikmatul Maula; Yeni Avidhatul Husnah; Previta Zeizar Rahmawati
Jurnal Laboratorium Khatulistiwa Vol 9, No 2 (2026): MEI 2026
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i2.2161

Abstract

Hemoglobin (Hb) is an essential parameter in oxygen transport and can be affected by occupational and environmental factors, including working duration. Parking attendants at Gondanglegi Market, Malang Regency, are exposed to prolonged working hours, vehicle emissions, and high physical activity, which may contribute to decreased hemoglobin levels. Previous studies have reported that occupational exposure to air pollution and long-term workload may negatively influence hematological status. However, studies focusing on hemoglobin levels among informal sector workers, particularly parking attendants, remain limited. This study aimed to analyze the hemoglobin levels of parking attendants based on their working duration at Gondanglegi Market, Malang Regency. A descriptive quantitative study design was employed using a total sampling technique involving 25 respondents. Blood samples were collected from parking attendants, and hemoglobin levels were measured using a photometer with the Cyanmethemoglobin method at the Bululawang Public Health Center Laboratory. The findings revealed that most respondents with a working duration of less than 10 years had normal hemoglobin levels, whereas respondents with more than 10 years of service tended to have hemoglobin levels below the normal range. These findings indicate that prolonged occupational exposure and longer working duration may contribute to decreased hemoglobin levels among parking attendants.
Association of Knowledge and Attitudes with Compliance to Standard Operating Procedures for Infectious Material Handling Among Clinical Pathology Laboratory Personnel Paulina Bekti Setiati; Putu Ayu Parwati; Diah Prihatiningsih
Jurnal Laboratorium Khatulistiwa Vol 9, No 2 (2026): MEI 2026
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i2.2259

Abstract

Compliance with Standard Operating Procedures (SOPs) for handling infectious materials is essential for preventing laboratory-acquired infections and ensuring occupational safety among laboratory personnel. Although knowledge and attitudes are commonly considered determinants of compliance, evidence regarding their influence on adherence to infectious material handling procedures remains inconsistent. This study aimed to examine the relationship between knowledge and attitudes of Clinical Pathology Laboratory personnel and compliance with SOPs for handling infectious materials at Buleleng District General Hospital. An analytical cross-sectional study was conducted involving all Clinical Pathology Laboratory personnel (n = 31). Data were collected using validated questionnaires assessing knowledge and attitudes, while SOP compliance was evaluated through direct observation. Statistical analysis was performed using the Spearman Rank correlation test. The findings revealed that 80.6% of respondents had good knowledge, 51.6% demonstrated positive attitudes, and 87.1% were compliant with infectious material handling SOPs. Spearman correlation analysis showed no significant association between knowledge and SOP compliance (p = 0.312) or between attitudes and SOP compliance (p = 0.269). These findings indicate that compliance with infectious material handling SOPs may be influenced more by situational and organizational factors, including workload, time constraints, work habits, availability of facilities and personal protective equipment, and supervisory practices, rather than by knowledge and attitudes alone. Strengthening organizational support and safety culture may therefore be necessary to improve SOP compliance in clinical laboratory settings.
Kasus Malaria di Kabupaten Nabire Papua Tengah Nurlaili Farida Muhajir; Arum Nuryati; Desto Arisandi; Audiah Fitriani; Hieronymus Rayi Prasetya; Fitri Nadifah
Jurnal Laboratorium Khatulistiwa Vol 9, No 2 (2026): MEI 2026
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i2.2300

Abstract

Malaria is an infectious disease caused by protozoan parasites of the genus Plasmodium that infect and multiply within human red blood cells. Papua Province is one of the regions with a high malaria burden in Indonesia, and Nabire Regency remains a malaria-endemic area. This study aimed to describe the characteristics of malaria cases at Nabire Regional General Hospital, Papua, during November–December 2023. This descriptive study used 78 blood smear preparations collected from patients at Nabire Regional General Hospital. Microscopic examination was conducted at the Clinical Laboratory of STIKES Guna Bangsa, Yogyakarta, during February–March 2024. Data were analyzed descriptively using SPSS version 16.0 and presented as frequencies and percentages. The results showed that 28 samples (35.9%) were positive for malaria and 50 samples (64.1%) were negative. The identified Plasmodium species were Plasmodium falciparum in 13 cases (46.4%), Plasmodium vivax in 11 cases (39.3%), and mixed infections in 4 cases (14.3%). Malaria cases were more common among females (20.5%) than males (15.4%). The highest proportion of cases was found among adults aged 26–45 years (17.9%) and residents of Nabire District (10.3%). In conclusion, Plasmodium falciparum was the predominant species identified, and malaria cases were more frequently found among females, adults aged 26–45 years, and residents of Nabire District.

Page 17 of 18 | Total Record : 171

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