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Jurnal Laboratorium Khatulistiwa
Published by Poltekkes Kemenkes Pontianak
ISSN : 25979523     EISSN : 25979531     DOI : https://doi.org/10.30602/jlk
Core Subject : Health, Science, Education,
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of Medical Laboratory. Jurnal Laboratorium Khatulistiwa particularly focuses on the main problems in the development of the Medical Laboratory health areas as follows: Toxicology Immunoserology Bacteriology Clinical Chemistry Parasitologi Micology And other related disciplines.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Imunologi dan Mikrobiologi (Lain-Lain)
Articles 156 Documents
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Daya Hambat Sediaan Deodoran Spray Minyak Atsiri Kulit Jeruk Bali Terhadap Bakteri Staphylococcus aureus Suwandi, Edy; Saputra, Daniel Angkasa; Kamilla, Laila; Sari, Emilda
Jurnal Laboratorium Khatulistiwa Vol 8, No 2 (2025): Mei 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i2.1832

Abstract

Deodoran spray adalah sediaan kosmetika yang digunakan untuk menyerap keringat dan menutupi bau badan. Bau badan merupakan masalah yang cukup penting, bau badan ini dapat ditimbulkan karena kurang menjaga kebersihan badan dan adanya aktivitas bakteri Staphylococcus aureus. Jeruk bali memiliki senyawa metabolit sekunder terpenoid. Senyawa metabolit sekunder tersebut dapat dimanfaatkan sebagai agen antibakteri pada sediaan deodoran spray. Tujuan penelitian ini untuk menganalisis perbedaan sediaan deodoran spray minyak atsiri kulit jeruk bali pada konsentrasi 3%, 6% dan 9% dalam menghambat pertumbuhan bakteri Staphylococcus aureus. Penelitian ini berbentuk Quasi Experimental Design, populasi pada penelitian ini adalah sediaan deodoran spray minyak atsiri kulit jeruk bali dengan menggunakan teknik sampling purposive. Sampel yang digunakan adalah sediaan deodoran spray minyak atsiri kulit jeruk bali konsentrasi 3%, 6% dan 9% yang dibuat 9 kali pengulangan pada masing- masing perlakuan, sehingga total sampel yang digunakan untuk 3 kelompok perlakuan tersebut adalah 27 sediaan deodoran spray yang diuji kekuatan antibakterinya dalam menghambat pertumbuhan bakteri Staphylococcus aureus dengan menggunakan metode difusi cakram Kirby-Bauer. Hasil uji daya hambat sediaan deodoran spray minyak atsiri kulit jeruk bali terhadap bakteri Staphylococcus aureus didapatkan rata-rata zona hambat pada konsentrasi 3% sebesar 7,1 mm, konsentrasi 6% sebesar 7,9 mm dan konsentrasi 9% sebesar 8,7 mm. Dari hasil analisis data uji Friedman diperoleh p-value 0,000 < α 0,05. Sehingga terdapat perbedaan antara setiap konsentrasi sediaan deodoran spray minyak atsiri kulit jeruk bali terhadap pertumbuhan bakteri Staphylococcus aureus.
Identifikasi Merkuri Pada Produk Pemutih di Kota Bekasi Purwanti, Angki; Prasetyorini, Tri; Mujianto, Bagya
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2115

Abstract

The BPOM regulations prohibit the use of mercury in cosmetics. Previous studies have reported mercury compounds in illegal skin-whitening products widely used by the public. This study aimed to identify mercury in facial whitening products circulating in the districts of Pondok Gede, Pondok Melati, and Jatiasih in Bekasi City. The study also traced distribution sites and recorded the presence or absence of BPOM registration numbers on products containing mercury. A descriptive study was conducted on 25 brands of facial whitening products. Qualitative mercury analysis was carried out using KI, NaOH, and the amalgam test. Testing was conducted from July to December 2024 at the Chemistry Laboratory of Poltekkes Jakarta III. Samples were considered positive if all three tests showed indicative color changes. The results showed that 12 brands (48%) contained mercury, and none had BPOM registration numbers. These products were obtained from terminals (16%), drugstores (12%), markets (12%), and plazas (8%), with none found in pharmacies. Meanwhile, 13 brands (52%) did not contain mercury, and all had BPOM registration numbers. A correlation was observed between mercury content and the presence of BPOM registration numbers. In conclusion, illegal cosmetics require serious attention due to potential mercury contamination. Terminals, markets, drugstores, and plazas were common distribution sites for these products. Consumers are advised to check the BPOM registration number before purchasing facial whitening products.
Pengaruh Sediaan Deodoran Krim Kombinasi Destilat Daun Sirih Hijau (Piper betle L.) dan Kulit Kayu Manis (Cinnamomum burmannii) Terhadap Pertumbuhan Staphylococcus aureus Mulyani, Elma Tri; Suwandi, Edy; Sari, Emilda
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2132

Abstract

Cream deodorant is a topical preparation used to reduce body odor caused by Staphylococcus aureus bacteria in sweat glands. The purpose of this study was to determine the effect of a cream deodorant preparation containing a combination of green betel leaf and cinnamon bark at concentration of 10%, 20%, and 30%  on inhibiting the growth of Staphylococcus aureus bacteria. Synthetic deodorants containing alumunium chloralhydrate have the potential to cause negative side effects, including cancer risk. Essential oils from green betel leaf and cinnamon bark contain antibacterial compounds such as betephenol and cinnamaldehyde, which work synergistically as active ingredients. This study used a purposive sampling technique and the well diffusion method on MHA medium. 10 replications were conducted, resulting in 30 samples. The results showed that the inhibition zone at a concentration of 10% is on average 13,4 mm (strong), at a concentration of 20%, it is on average 20.5 mm (very strong) and at a concentration of 30%, it is on average 23,5 mm (very strong). The results of a simple linear regression statistical test showed a significance value (p=0.000 → p < 0.05), indicating a significant effect of concentration on inhibitory power. This study concludes that the cream deodorant preparation containing the combination of green betel leaf and cinnamon bark distillate was effective in inhibiting Staphylococcus aureus, with the highest inhibition zone at a concentration of 30%. This combination has the potential to be developed as a safe, natural antibacterial deodorant ingredient.
Identification of Fungal Agents Causing Onychomycosis in Elderly at Banjar Dinas Batang, Bali Rahayu, Ni Putu Indira; Prasetya, Didik; Abadi, Moh. Fairuz
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.1983

Abstract

Onikomikosis merupakan infeksi jamur pada kuku yang sering terjadi pada lansia akibat faktor usia, lingkungan lembap, dan kebersihan yang kurang. Penelitian ini bertujuan untuk mengidentifikasi jenis jamur penyebab onikomikosis dan mengevaluasi karakteristik infeksi pada lansia di Banjar Dinas Batang, Karangasem, Bali. Metode yang digunakan adalah deskriptif dengan pendekatan observasional dan pemeriksaan kultur jamur. Sebanyak 30 lansia dipilih melalui purposive sampling dan dilakukan pengambilan sampel kuku untuk pemeriksaan laboratorium menggunakan media Sabouraud Dextrose Agar serta pewarnaan lactophenol cotton blue. Hasil pemeriksaan menunjukkan bahwa 2 dari 30 sampel (6,67%) positif terinfeksi jamur, dengan spesies dominan Trichophyton mentagrophytes. Mayoritas responden perempuan (57%) menunjukkan angka infeksi lebih tinggi dibandingkan laki-laki (43%). Faktor risiko utama meliputi kebersihan personal yang buruk dan paparan lingkungan lembap. Temuan ini mendukung studi sebelumnya bahwa lingkungan tropis dan usia lanjut berkontribusi terhadap peningkatan prevalensi onikomikosis. Meskipun prevalensinya rendah, infeksi ini tetap berisiko menurunkan kualitas hidup lansia. Oleh karena itu, edukasi kebersihan kuku dan pemeriksaan berkala sangat dianjurkan untuk pencegahan. Penelitian ini juga merekomendasikan penggunaan metode diagnostik molekuler pada studi lanjutan untuk meningkatkan akurasi identifikasi jamur penyebab onikomikosis. 
Kandungan Formalin dan Jumlah Total Bakteri pada Produk Sosis di Pasar Tradisional Moga Kabupaten Pemalang Jawa Tengah Alimi, Ramadan Nur; Ambarwanty Mohtar, Cici Farhana; Inayah, Mazidah Noer
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2091

Abstract

One sort of frozen food that is made from processed meat is sausage. Formalin is one of preservative that is frequently abused, which can found in market-sold sausages. Health issues can arise from consuming foods containing formalin for an extended period of time. The filthy market environment, dust, and touch between customers' and sellers' hands can all lead to microbial contamination of food that sold in traditional markets. There has never been any prior research on the formalin content and bacterial contamination of sausage products sold in Pemalang Central Java's traditional markets.  Therefore, the purpose of this study was to figure out the amount of formalin and the quantity of bacteria contaminating sausage products that are sold at Moga traditional market in Pemalang, Central Java.  There were 6 samples of sausage were chosen randomly from several suppliers. A formalin test kit was used to qualitatively analyze the formalin, and the TPC method was employed for counting the bacteria. The result showed that formalin was absent from every sausage products. However, the TPC indicated that 4 of 6 sausages were contaminated with bacteria, S01, A02, S03, and S06, with counts of 1,2 × 10⁴ CFU/mL, 8.6 × 10⁴ CFU/mL, 8.7 × 10⁴ CFU/mL, and 3 × 10⁴ CFU/mL, respectively. Fortunately, the overall bacterial count remained below the acceptable level for human consumption.
Anti-Inflammatory Activity Test of Tapak Liman (Elephantopus scaber L.) Leaf Extract Using Red Blood Cell Membrane Stabilization Method Wahdaniah, Wahdaniah; Yanti, Rahmi; Kamilla, Laila; Salim, Maulidiyah
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2135

Abstract

Inflammation is the reaction of living tissue to trauma or infection, both in acute and chronic conditions. One plant with anti-inflammatory potential is the tapak liman leaf (Elephantopus scamber L.), which contains secondary metabolites such as phenolics, flavonoids, tannins, alkaloids, steroids/treponoids, and saponins. The purpose of this study was to determine the anti-inflammatory activity of Elephantopus scamber L. leaf extract at concentrations of 0.005%, 0.01%, 0.02%, 0.04%, and 0.08% using the red blood cell membrane stability method. Anti-inflammatory activity showed that 70% ethanol extract of tapak liman leaves had low anti-inflammatory activity at a concentration of 0.005% with a stability of 98.00%, while the highest activity was found at a concentration of 0.08% with a stability of 99.65%. Microscopic examination showed that at concentrations of 0.005%, 0.01%, 0.02%, 0.04%, and 0.08%, the administration of tapak liman extract could restore the normal shape of erythrocytes. The Kruskal-Wallis test yielded a significance value of 0.000, which is less than 0.05, meaning that Ha is accepted. It can be concluded that there are differences in the anti-inflammatory activity of tapak liman leaf extract (Elephantopus scamber L.) at concentrations of 0.005%, 0.01%, 0.02%, 0.04%, and 0.08%.
Antibacterial Activity of Ethanol Extract Ointment of Tithonia diversifolia Against Staphylococcus aureus Triana, Linda; Supardi, Rizal; Sutriswanto, Sutriswanto; Ratnawati, Gervacia Jenny; Sari, Emilda; Nurhayati, Etiek
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2136

Abstract

Skin infections caused by pathogenic bacteria such as Staphylococcus aureus remain a common health problem, and the need for safer alternative topical antibacterial agents continues to increase. This study aimed to evaluate the antibacterial activity of ethanol extract ointment of Tithonia diversifolia leaves with various concentrations formulated as a semi-solid topical preparation. A quasi-experimental design was used, and ointments were prepared at concentrations of 5%, 7%, 9%, 11%, 13%, 15%, 17%, 19%, and 21%. Antibacterial activity was tested using the well-diffusion method against Staphylococcus aureus. A total of 27 samples were tested with three replications. The results showed that the ointment produced inhibition zones ranging from 13.83 mm to 22.00 mm, with higher concentrations yielding greater inhibition. Repeated Measures ANOVA showed a significant difference among concentrations (p = 0.003), indicating that the increase in extract concentration significantly affected the antibacterial effectiveness. This study concludes that ethanol extract ointment of Tithonia diversifolia leaves demonstrates strong antibacterial activity against Staphylococcus aureus*, with effectiveness increasing proportionally with concentration.
Analisis Korelasi HbA1c dengan Mean Platelet Volume dan Platelet Distribution Width pada Pasien Diabetes Melitus Tipe 2 Azzahra, Andi Dinda; Anhar, Citra Amaniah; Hardianto, Nova
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2169

Abstract

Controlling blood glucose levels is the main key in treating and preventing diabetic vascular complications. However, routine monitoring of glycemic status using standard HbA1c markers is still hampered by limited costs and health facilities. The platelet index is considered to have potential as a predictor of vascular complications in DM patients. This study aims to examine the correlation of HbA1c with mean platelet volume (MPV) and platelet distribution width (PDW) in type 2 DM patients. This cross-sectional study used medical record data. The sample consisted of 60 patients with type 2 diabetes who received treatment at Pasar Rebo Regional General Hospital, Jakarta, in 2024. All patients had HbA1c levels above 6.5%. The proportion of patients with high MPV and PDW was 5% and 11.7%, respectively. Based on bivariate analysis with the Spearman test, there was a significant correlation between HbA1c levels and MPV values (p-value < 0.05; r = 0.561) and PDW values (p-value < 0.05; r = 0.433). A strong positive correlation was observed between HbA1c levels and MPV values and a moderate positive correlation between HbA1c levels and PDW values, indicating that an increase in HbA1c levels tends to be accompanied by an increase in MPV and PDW values.
Pre-Analytical Stability of Fasting Blood Glucose: A Comparison Between Immediate Analysis and Two-Hour Room Temperature Storage Sugito, Sugito; Djohan, Herlinda; Tumpuk, Sri; Sidiq, Alfino Validita; Agum, Dexa
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2138

Abstract

The stability of blood samples for glucose testing is a critical preanalytical factor. In vitro glycolysis at room temperature can significantly decrease glucose levels, potentially leading to inaccurate diagnostic results. This study aimed to compare fasting blood glucose levels examined immediately and those stored for 2 h at room temperature. This descriptive, cross-sectional study involved 35 serum samples from participants undergoing fasting blood glucose tests at the Sintang Regional Health Laboratory. Each sample was split into two aliquots: one was analyzed immediately after processing (baseline), and the other was analyzed after being stored for 2 hours at room temperature (25-30°C). Glucose levels were measured using the GOD-PAP method with a Microlab 300 spectrophotometer. Statistical analysis was performed using the Shapiro-Wilk normality test and paired T-test to compare the differences in glucose levels between the two groups. The mean glucose level examined immediately was 90.8 mg/dL (SD=5.098), while the level after the 2-hour delay was 85.8 mg/dL (SD=4.451), showing an average decrease of 5.0 mg/dL. The data were normally distributed (p=0.129), and the paired T-test showed a highly statistically significant difference (p<0.001) between the two examination conditions. Storing serum samples at room temperature for 2 h significantly decreased glucose levels. This finding underscores the importance of immediate sample processing for glucose testing to ensure accurate diagnosis. If a delay is unavoidable, sample refrigeration is highly recommended to inhibit the glycolysis process.
In Vitro Anti-Biofilm Activity of Nutmeg (Myristica fragrans) Leaf Infusion Extract Wijayanti, Faulia Triana; Nugroho, Yusuf Eko; Pangesti, Ira
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2097

Abstract

Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium commonly associated with severe infections, particularly in immunocompromised individuals. One of its major virulence factors is biofilm formation, which contributes to increased antimicrobial resistance. This study aimed to evaluate the inhibitory activity of nutmeg (Myristica fragrans) leaf infusion extract against P. aeruginosa biofilm formation in vitro. The experiment was conducted using a 96-well microtiter plate assay, and biofilm intensity was quantified at 595 nm following crystal violet staining. The results demonstrated that the nutmeg leaf infusion extract significantly inhibited biofilm formation, with varying levels of effectiveness depending on incubation time. These findings indicate the potential of nutmeg leaf infusion extract as an antibiofilm agent against P. aeruginosa.

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