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INDONESIA
Jurnal Serambi Biologi
Published by Universitas Negeri Padang
ISSN : -     EISSN : 27222829     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Artikel ilmiah yang dipublikasi adalah artikel dalam bidang biologi (biodiversitas, biosistematika, ekologi, fisiologi, genetika dan bioteknologi, biokimia) yang meliputi semua bentuk mahluk hidup mulai dari mikroba, fungi, tumbuhan, hewan, manusia dan virus
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biologi Perkembangan
Articles 187 Documents
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Amphibious Inventory in Borneo Indonesia Through the AMNH Platform Heafiz, Elviona; zega, Dwi; Hayyat, Rafikul; Utia, Ulta; Satria, Rijal; Nugraha, Fitra
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.322

Abstract

Borneo is the 3rd largest island in the world with an area of 748,168 km2. Indonesia which has a high biodiversity value. Amphibians are one of the constituent components of the ecosystem that has a very important role. This study aims to determine the number and diversity of amphibians on the island of Kalimantan. This research was conducted by collecting data through the AMNH (Amphibian Museum of Natural History) Platform. Based on data collected through the AMNH platform, there were 10 families, namely Megophryidae as many as 13 species, Mycrohilidae as many as 16 species, Ranidae as many as 19 species, Dicroglossidae as many as 15 species, Bombinatoridae as many as 2 species, Ichthyophiidae as many as 2 species, Ceratobatrachidae as many as 2 species, Bufonidae as many as 6 species, and Rhacophoridae as many as 16 species. So the total amphibians on the island of Borneo based on the data we obtained through the AMNH platform is 91 species.
ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT (BAL) PADA FERMENTASI DURUAN TEMBAGA (Durio zibethinus Murr.) DARI PADANG PARIAMAN SUMATERA BARAT Fadila, Sharah Iza
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.323

Abstract

Bakteri asam laktat adalah kelompok bakteri yang memproduksi asam laktat sebagai produk metabolik. BAL adalah bakteri gram positif berbentuk kokus, batang tidak berspora dan bersifat mikroaerofilik. BAL dapat diisolasi dari berbagai olahan pangan fermentasi salah satunya adalah tempoyak. Tempoyak merupakan produk olahan buah durian yang dibuat dengan cara fermentasi spontan secara anaerob selama 3–7 hari. Fermentasi tempoyak melibatkan bakteri asam laktat (BAL) yaitu jenis bakteri yang dapat menghasilkan asam laktat, hidrogen peroksida, antimikroba, dan hasil metabolisme lain yang memberikan pengaruh positif bagi produktivitas makanan terutama makanan fermentasi. Tujuan dari penelitian ini adalah untuk mengisolasi bakteri asam laktat (BAL) dari fermentasi durian tembaga. Penelitian ini merupakan penelitian deskriptif. Isolasi dan Karakterisasi BAL dilakukan dengan metode Total Plate Count (TPC) menggunakan medium selektif MRSA. Karakterisasi BAL dilakukan dengan pengamatan mikroskopis dan makroskopis dengan metode pewarnaan Gram. Berdasarkan penelitian yang telah dilakukan didapatkan 3 Isolat BAL. pengamatan makroskopis diperoleh koloni bentuk bulat (circular), tepian licin (entire), elevasi (convex dan raised) dan berwarna putih susu. Pengamatan mikroskopis diperoleh koloni bakteri Gram positif berbentuk sel batang (bacill)
Literatur Review: Koenih Rimbo (Curcuma sumatrana Miq.) Tumbuhan Endemik Terancam Punah Pulau Sumatra yang Berpotensi Sebagai Tumbuhan Obat Citaindah, Ameilia; Leilani Eka Putri, Irma
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.324

Abstract

Minat masyarakat Indonesia terhadap penggunaan obat tradisional sangat tinggi, dikarenakan penggunaan tanaman obat dipercaya dapat meningkatkan sistem imun tubuh. Koenih Rimbo (Curcuma sumatrana Miq.) merupakan salah satu tumbuhan obat endemik Sumatra yang berasal dari family Zingiberaceae. Namun, saat ini Koenih Rimbo masuk ke dalam Daftar Merah Spesies Terancam Punah IUCN Red List pada tahun 2018. Koenih Rimbo memiliki nilai penting dalam bidang pengobatan tradisional. Penelitian ini bertujuan untuk mengetahui morfologi, fisiologi, habitat dan potensi nya sebagai tumbuhan obat. Jenis penelitian ini adalah penelitian yang menggunakan literature review. Berdasarkan artikel yang dikumpulkan didapatkan hasil bahwa C. sumatrana merupakan tanaman herba yang memiliki tinggi 135 cm dan memiliki rimpang berbetuk bulat seperti telur. C. sumatrana habitatnya dihutan sekunder yang berada pada ketinggian 100-500 mdpl. C. Sumatra merupakn tumbuhan obat yang memiliki potensi meningkatkan meningkatkan kecerdasan, struktur histologi otak dan konsentrasi malondialdehid pada Mencit, sebagai antikanker dan sebagai antibakteri, hal ini dikarenakan terdapat berbagai macam senyawa aktif pada rimpang C. sumatrana.
Artikel Review: Kajian Perilaku Gajah Sumatera (Elephas maximus sumatranus) di Taman Margasatwa Sa'diah, Jihan Natul Sa'diah; Atifah, Yusni
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.326

Abstract

Gajah Sumatera (Elephas maximus sumatranus) merupakan mamalia besar yang tersebar sepanjang Pulau Sumatera. Kajian terhadap perilaku gajah Sumatera yang mencakup perilaku khas individu gajah sangat penting untuk mendukung kegiatan ekowisata. Informasi perilaku gajah diperoleh melalui observasi ilmiah disajikan kepada wisatawan untuk memberikan wawasan dan pengetahuannya selama berkunjung ke Taman Margasatwa. Taman Margasatwa merupakan salah satu lembaga konservasi ek-situ yang memiliki koleksi satwa gajah Sumatera. Pada artikel ini, penulis mengumpulkan informasi tentang perilaku gajah Sumatera berdasarkan jenis kelamin, umur, asal penangkapan, lama pelatihan, serta mengkaji perilaku gajah dalam mendukung kegiatan ekowisata di Taman Margasatwa. Metode yang digunakan dalam artikel ini adalah studi literatur dengan menganalisis atau mereview 10 jurnal dengan menggunakan tiga database yaitu Google Scholar, Science Direct, dan Pubmed. Ulasan ini memberikan informasi yang menunjukkan gajah jantan memiliki respon yang lebih agresif dibandingkan gajah betina. Serta lama pelatihan juga mempengaruhi perilaku gajah. Penulis berharap ulasan ini dapat memberikan pengetahuan dan wawasan terkait perilaku gajah Sumatera di Taman Margasatwa.
Uji Sensitivitas Antibiotik Mikroba Tanah Pertanian yang Terpapar Disinfektan Di Kecamatan Batang Anai Kabupaten Padang Pariaman Chania, Suci Febri; Zega, Dwi; Putri, Dwi
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.327

Abstract

Indonesia merupakan negara agraris yang mengandalkan sektor pertanian sebagai sumber penghasilan untuk memenuhi kebutuhan pangan masyarakat. Keberadaan hama di area budidaya tanaman pertanian mengancam stabilitas produksi dan produktivitas tanaman. Petani umumnya menggunakan pestisida kimiawi setiap terjadi serangan hama dan penyakit pada pertanian,bahkan petani menggunakan pestisida tanpa memperhatikan hama target, cenderung berlebihan, dan tidak tepat baik jenis, dosis, serta metode aplikasi yang digunakan. Penggunaan bahan-bahan agrokimia sintetik seperti pesstisida berpotensi menngakibatkan adanya residu bahan tersebut di dalam tanah yang secara langsung maupun tidak langsung berpengaruh pada populasi dan aktivitas mikroba tanah sehingga mempengaruhi stabilitas ekosistem serta mempengaruhi keseimbangan kompleks jerapan di dalam tanah. Hal ini memungkinkan untuk mempegaruhi keseimbangan hara atau kadar kemasaman tanah. Oleh karena itu, dilakukanlah penelitian ini yang bertujuan untuk menguji sensitivitas antibiotik terhadap bakteri tanah terpapar disinfektan di Batang anai, Kabupaten Padang Pariaman, Sumatera Barat. Berdasarkan hasil penelitian yang telah dilakukan, didapatkan hasil bahwa Zona hambat yang terbentuk memiliki rata-rata yang tinggi yang membuktikan bahwa bakteri tanah sawah di Kecamatan Batang Anai Kabupaten Padang Pariaman tersebut masih sensitif terhadap antibiotik dan memiliki kualitas tanah yang baik.
Comparison of Boiling Methods for Bacterial DNA Isolation Using Waterbath and Heatblock Sirwati, Fadila; Nurfalinda, Siti; Salsabila, Siti; Putri, Dwi
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.328

Abstract

Molecular biology analysis has several advantages over culture approaches, including the ability to identify a wider range of target organisms, increased sensitivity and specificity. Bacterial DNA isolation is a simple solution in molecular biology analysis. Boiling techniques using high temperature heating can damage cell wall permeability. Heating boiling technique can be done by using waterbath, and heat block with different heat transfer mechanism. The results showed the concentration value of samples isolated using headblock was in the range of 77.6 ug/ul - 200.45 ug/ul, while the concentration of samples isolated using waterbath was in the range of 145.575 ug/ul - 288.8 ug/ul. The highest DNA concentration was obtained from using a waterbath. Purity values measured at wavelengths A260/A280 were obtained with a range of purity that was not different.
Vegetation Distribution Patterns in the Mangrove Forest Area of Teluk Buo Tourism Village, West Sumatera Marbun, Duma Erza; Putri, Irna Leilani Eka
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.330

Abstract

Vegetation is a plant community that can be found in a landscape or geographic area. Vegetation has the ability to adapt to its environment and has an important role in terms of hydrology and climate change. Teluk Buo Beach, which is located in Teluk Buo Tourism Village, Teluk Kabung Tengah Village, Bungus District, Padang City, West Sumatra, has various types of coastal vegetation. The vegetation that can be found in the Teluk Buo Beach area is herbaceous vegetation, shrubs and trees. Dispersal Pattern is a movement separating individuals or population groups from the vegetation population itself and this movement is closely related to environmental conditions. This research aims to determine the distribution pattern of vegetation on Teluk Buo Beach, West Sumatra. The research method implemented was the quadratic method. The vegetation found was 7 species, namely Rhizophora apiculata, Bruguiera gymnorhiza, Setaria palmifolia, Nypa Fruticans, Bruguiera gymnorhiza, Euterpe oleracea mart., and Lumnitzera littorea. In herbaceous habitus vegetation, the distribution pattern is random with a morisita degree value obtained at 0.23, namely IP = 0, for bush and tree habitus vegetation, the morisita degree value obtained is -11.91 and -12.47 based on the criterion of P < 0, namely distribution pattern. uniformly.
Genetic Variation Analysis of the E6 HPV 16 Gene Using RFLP In Silico Annisa, Silvy; Rahmawati, Atika Ayu; Nadira; Khairani, Fidia Aura; Achyar, Afifatul
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.331

Abstract

Cervical cancer is cancer that attacks epithelial cells or the outer surface layer of the cervix. This cancer is most commonly caused by the high risk type of Human Papillomavirus. The RFLP method uses restriction enzymes to cut certain nucleotide sequences at specific positions that produce fragments of different lengths. The purpose of this study was to determine the polymorphism that occurs in the E6 gene of the HPV 16 virus. This research was conducted using RFLP in silico. Virtual descriptive methods are used to analyze data and collect information about the object of study. The restriction enzyme HpaII was used in this study. The results showed that there were two alleles (A1 and A2) out of a total of 15 sequences of the E6 HPV 16 gene in Popset 636528409 indicating that there was a genetic variation in the gene.
Contribution of Goat Manure Organic Fertilizer to the Number of Rice (Oryza sativa L.) Tillers Sunarsan, Debra Fortuna; Anhar, Azwir; Kardiman, Reki; Vauzia, Vauzia
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.332

Abstract

The relatively high and continuous use of inorganic fertilizers in rice cultivation can cause negative impacts on the environment. Minister of Agriculture Regulation Number: 40/2007 recommends providing organic fertilizer combined with inorganic fertilizer with the aim of improving soil condition and fertility. This research aims to determine the effect of the composition of organic goat manure and inorganic fertilizer on the number of rice tillers. This research was structured using a Completely Randomized Design. The treatment given was a comparison of organic fertilizer to inorganic fertilizer consisting of 6 treatments and 4 replications. The treatments given consisted of A(100% NPK Fertilizer + 0% Goat Manure Fertilizer), B(80% NPK Fertilizer + 20% Goat Manure Fertilizer), C(60% NPK Fertilizer + 40% Goat Manure Fertilizer), D(40% NPK Fertilizer + 60% Goat Manure Fertilizer), E(20% NPK Fertilizer + 80% Goat Manure Fertilizer), F(0% NPK Fertilizer + 100% Goat Manure Fertilizer). Observational data was analyzed by analysis of variance (ANOVA) using the SPSS application. The results showed that the use of inorganic NPK fertilizer combined with organic goat manure had no significant effect on the number of offspring. The composition of inorganic NPK fertilizer and organic goat manure gave the same response to the number of rice tillers.
Genetic Variation Analysis of katG Gene Sequence in Mycobacterium tuberculosis Using RFLP In Silico Nurfalinda, Siti; Aulia, Ony Nattasha; Sirwati, Fadila; Alridho, Rezeki Rival; Achayar, Afifatul
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.337

Abstract

Tuberculosis (TB) is an infectious disease that is the leading cause of death in Indonesia. This infection is caused by Mycobacterium tuberculosis. In Indonesia in 2021, there were approximately 824,000 cases of TB. A total of 8,268 TB cases were associated with resistance of M. tuberculosis to anti-tuberculosis drugs (OAT). MDR-TB is a strain that is resistant to two of the most important anti-tuberculosis drugs in first-line TB therapy, namely Rifampicin (RIF) and Isoniazid (INH). The most common mechanism of INH resistance is the katG gene mutation, found (95%) at codon 315. In the katG gene there is a change in the amino acid Ser (AGC) → Thr (ACC). Genetic variation in a population will affect the survival ability of an individual. This study used BamHI restriction enzyme. This study aims to analyze genetic variation in the KatG gene sequence in M. tuberculosis NCBI popset 2363230696 by using RFLP in silico. The results showed that there were genetic variations in the KatG gene sequence and two allelic variations contained in 7 KatG gene sequences using the BamHI restriction enzyme. Key words : M. tuberculosis, BamHI, KatG , Genetic Variation, RFLP in silico.

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