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INDONESIA
IJOBI
Published by Universitas Negeri Yogyakarta
ISSN : 30320216     EISSN : 30320194     DOI : https://doi.org/10.21831
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Neuroscience
This journal publishes manuscripts of research articles and studies related to Biology including Zoology, Botany, Genetics, Microbiology, Ecology, and Applied Biology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 15 Documents
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MOLECULAR IDENTIFICATION OF A LOCAL LACTIC ACID BACTERIA (LAB) ISOLATE (B21) AND PRIMER CONFIRMATION FOR D-Lactate Dehydrogenase (D-LDH) GENE ISOLATION Madhani, Anisa Tiara; Nurcahyo, Heru; Octavia, Bernadetta; Astuti; Mercuriani, Ixora Sartika
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 1 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i1.104

Abstract

This study aims to identify a lactic acid bacteria (BAL) isolated from native chicken (Gallus gallus domesticus) intestinum (B21) based on 16S rRNA gene sequence and also to confirm primers for PCR-based D-Lactate dehydrogenase (D-LDH) gene isolation. The genomic DNA of B21 was isolated then amplified using 16S rRNA. The PCR product then sequenced and aligned. The sequencing was done in 1st Base Pte. Malaysia. The sequence then aligned using the Basic Local Alignment Search Tool (BLAST) in NCBI to know the similar genome species. Isolation D-LDH gene was done through PCR using 5 primers (idb 0101, idb 1147, idb 0813, idb 1010, and idb 2021) of B21 and also J15 isolate genome. The PCR product were then sequenced and aligned with the D-LDH gene sequences in NCBI. The results of molecular identification based on 16S rRNA markers showed that B21 has 99.45% similarity to Bacillus proteolyticus. Amplification of the LDH gene with idb 1010 primer on J15 isolate yielded 4 fragments (i.e. 650, 1400, 1500, and 1700bp). The results of phylogenetic tests showed that the 1700bp fragment has a high similarity to the D-LDH gene in Myobacterium tuberculosis species with a bootstrap value of 84%. While other size fragments cannot be trusted to have similarity to LDH sequence because they have not reached the minimum bootstrap value that meets the qualifications.
THE EFFECT OF DIFFERENT BIO ACTIVATORS ON QUALITY COMPOST OF AGRICULTURAL WASTE Pratiwi, Ellyza Yohana Putri; Suhartini; Sudrajat, Ahmad Kamal
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 1 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i1.105

Abstract

This study aims to determine the effect of using various bio activators on quality compost from agricultural waste and the best bio activators to obtain good quality compost from agricultural waste. This type of research is experimental with a Completely Randomized Design (CRD). The object of this research is compost from agricultural waste added with various bio activators: Tape Yeast, Tempe Yeast, Stardec, EM-4, and M-21 Decomposer. Each treatment consisted of 3 (three) replications. This study lasted for 75 days. The quality of compost is observed by physical parameters (temperature, humidity, pH, volume, weight, color, texture, smell), chemical parameters (carbon (C), nitrogen (N), C : N ratio, phosphorus (P), Kalium (K)), and composting time. Data were analyzed using One Way ANOVA and Kruskall Wallis Test. The results of this research on compost quality with physical parameters in all treatments had almost the same physical appearance, namely blackish brown color, texture or grain size of 2- 4mm, and had an earthy smell. Meanwhile, the analysis results on compost quality with chemicals showed a significant effect of different bio activators on the compost quality. Furthermore, the length of time for composting shows that the use of bio activators can affect the quality and speed up the composting time. EM-4 is the best bio activator to obtain the best compost quality.
EFFECTIVENESS OF MAJAPAHIT LEAF EXTRACT (Crescentia cujete L.) AS A NATURAL PESTICIDE FOR BANANA LEAF ROLLER CATERPILLAR (Erionota thrax L). Pratomo, Ibnu; Aminatun, Tien; Kuswandi, Paramita Cahyaningrum
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 1 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i1.106

Abstract

This study aims to determine the effectiveness of Majapahit leaf extract (Crescentia cujete L.) on the feeding activity and mortality of the banana leaf roller caterpillar (Erionota thrax L.), and also the optimal concentration that affects the feeding activity and mortality of the banana leaf roller caterpillar (Erionota thrax L.). This research is a completely randomized design (CRD) experiment with 4 treatments and 3 replicates. In thos study, 60 third instar larvae of Erionota thrax L. were taken using the transect method from several villages in Purworejo district, Central Java. The pesticide from Majapahit leaves was made through a simple extraction method with 4 concentrations: 0%, 25%, 30% and 35%. The results showed that Majapahit leaf extract had an effect on feeding activity and mortality of the banana leaf rolling caterpillar. The optimal concentration of Majapahit leaf extract that affects feeding activity and pest mortality was 35%.
CAVE BATS (YANGOCHIROPTERA AND YINPTEROCHIROPTERA) IN GUNUNG SEWU GEOPARK: STUDY OF KARST CAVES IN WONOGIRI REGENCY Az-Zahra, Nabilah; Alifah, Faidatun; Noorfamaza, Aura; Nurrohimah, Kholifah; Novitasari, Nabila; Erindra Maharani, Farella; Indira Oktaviani, Vincentia; Cahya Jasinda, Ridha; Arlianti Azzahra, Sevina; Debora Aprillia Siagian, Shella; Prakarsa, Tatag Bagus Putra
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 1 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i1.107

Abstract

Indonesia is a country rich in biodiversity including flora and fauna. One of the high fauna diversity in Indonesia is the mammal. Some of these bats use karst caves as their roosting habitat. One of the karst areas in Indonesia is Gunung Sewu Karst. This study aims to learn more about the various bats that live in caves in the Gunung Sewu Geopark Area. This research was conducted in March-May 2023, in several caves located in the Gunung Sewu Geopark Area. Bats are caught using misnets and handnets. After they were captured, the bats were identified using Morphometry and the Shannon-Wiener index. Through another index, the Margalef index, the bat diversity in the tree cave habitats was expressed, with a discovery that there are many different species. Based on the similarity index, bats were categorized again using cluster analysis and the unweighted pair-group method using arithmetic averages (UPGMA). The entire analysis was assisted with software PAST ver 4.13. The species recorded from the three caves in this study amounted to 8 species of bats belonging to 5 genera and 5 families. Sodong Cave is a habitat with the highest level of diversity and distribution of species. All three habitats have low similarity.  The existence of endemic and vulnerable species is a priority in protecting caves as their habitat and Gunung Sewu karst macroecosystems. By preserving the habitat, all biodiversity in it will be preserved.
PHENETIC DIVERSITY OF CELLULASE-PRODUCING BACTERIA FROM WANA TIRTA KULON PROGO MANGROVE FOREST Febriani, Heni; Octavia, Bernadeta; Zulaika, Enny
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 1 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i1.114

Abstract

Mangrove is an ecosystem that have important value for the environment and are a source of cellulase-producing microorganism biomass. The purpose of this study was to determine the characteristics and types, as well as the highest cellulase enzyme activity from cellulase-producing bacteria found in Wana Tirta Mangrove Forest, Jangkaran, Kulon Progo. This research is a descriptive-exploratory research. Sampling in the form of litter, mud and water was carried out in the Wana Tirta Kulon Mangrove Forest which was divided into 3 plots. This sample is then isolated on selective media in the form of Carboxymethyl cellulose (CMC) In order to grow cellulolytic bacteria. The bacteria obtained are then purified and phenetic characterization. The data obtained were used to classify the bacterial isolates using MVSP identification software (Multivariate Statistical Package) 3.1 with UPGMA clustering algorithm (Unweight Pair Group Method With Arithmatic Averages) then the result is presented in the form of a dendogram. Result Research shows as many as 17 isolates of cellulolytic bacteria were obtained. There were 4 bacterial isolates from litter samples, 1 bacterial isolate from water samples and 12 bacterial isolates from mud samples. After being made in the form of a dendogram, 19 bacterial clusters were obtained. A total of 6 bacterial isolates had a similarity index of ≥ 72% against Bacillus pumilus namely isolates S1A 2, S2A, AL 3, AP 3, AP 6 and AP 17, 4 bacterial isolates have a similarity index of ≥ 70% to Bacillus stearothermophyllus namely AP 22, AP 24, AP 25, and AP 26 isolates. As well as 7 bacterial isolates have a similarity index of ≥ 77% against Streptomyces Sp. namely isolates S1A 1, S3B, AP 8, AP 9, AP 14, AP 20 and AP 27.The highest cellulase enzyme activity occurred in AP 14 bacterial isolate of 286.72 U / ml.
Water Quality Of The Tirtoagung Reservoir Based On The Phytoplankton Diversity Index And The Saprobic Index In Sleman Yogyakarta Rahmayanti; Sudarsono
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 2 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i2.212

Abstract

Embung Tirtoagung is an artificial aquatic ecosystem as a supplier of agricultural land during the dry season and as a fishery facility in Sleman Regency. This study aims to determine the water quality in Tirtoagung Embung based on the diversity index and saprobic index. This research is an exploratory descriptive research by way of observation. The research was conducted in February – April 2021 during the rainy season. The sampling locations were at 5 stations selected by random sampling. The results of the study showed that the water quality in Tirtoagung Embung is included in the lightly polluted category. It is known from the calculation of the diversity index that is 2,391 so that the situation is in the moderate category and the Tirtoagung reservoir is in a lightly polluted condition. Then seen from the calculation of the saprobic index, which is 0.71 - 1.00 so that the condition of the waters of the Tirtoagung reservoir is in the - mesosaprobic phase with light pollution levels and few organic and inorganic compounds. In addition, the water quality of the Tirtoagung reservoir based on physical and chemical parameters is still feasible for the survival of phytoplankton.
The Effect of BAP and NAA Combination on Callus Induction of Aglaonema Siam Aurora Leaf Explants in Vitro Anjani, Dianita Dewi; Ratnawati
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 2 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i2.213

Abstract

This research aims to determine the effect of the Naphthalene Acetic Acid and Benzyl Amino Purine combination on callus growth of Aglaonema Siam Aurora leaf explants and to determine the combination level at the most optimal concentration on the callus growth of Aglaonema Siam Aurora leaf explants. Observation variables included callus emergence time, explants percentage of formed callus, callus length, and explant life percentage. The explants that where used were the first young leaves explants from shoots that were opened, with 2x2 cut size. Results show that the addition of a NAA and BAP combination had an effect on . Combination of 1.2 ppm BAP + 1 ppm NAA treatment is the most optimum treatment for callus growth with a callus percentage of 87.5%, the callus start time at week 3, the average callus length is 0.77 mm and the percentage number of survived explants is 100%
The Effect of Combination Psophocarpus Tetragonolobus and Pisum sativum Extract on The Histological Structure of The Liver, Kidney and Stomach of Rattus norvegicus Elfidariani, Ratih; Triharjana
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 2 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i2.214

Abstract

This research aim to determine the effect of the extract of winged bean (Psophocarpus tetragonolobus) and peas (Pisum sativum) toward histological structure of the liver, kidneys, and stomach of white rats (Rattus norvegicus). The objects of this research are twenty (20) white rats female wistar strain with ±100-200 grams of weigh which had never been pregnant. White rat samples are divided into 1 group as a control (P0) and 3 treatment groups with P1 25 mg/kgBB of winged bean extract + 75 mg / kgBB of peas extract, P2 50 mg / kgBB of winged bean extract + peas extract 50 mg / kgBB, and P3 winged bean extract 75 mg / kgBB + peas extract 25 mg / kgBB given for 21 days. Each group consist of 5 repetitions. The analysis used the One Way Anova test, it the results were significant, and then proceed with the Duncan's Multiple Range Test (DMRT) test. To determine the trend of the effect of winged bean seed extract and pea on the histological structure of the liver, kidneys, and stomach, descriptive analysis was performed. The results showed that the extract of winged bean and pea had no significant effect (p> 0.05) on the histological structural damage of the liver and kidneys of white rats. Giving winged bean extract) and peas extract on the histological structure of the stomach of white rats in all treatment groups was under normal conditions.
Antibacterial Activity of Green Meniran Extract (Phyllanthus niruri Linn) on The Growth of Salmonella typhimurium Nafisah, Aulia Erta; Octavia, Bernadetta; Lestari, Endang Gati
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 2 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i2.215

Abstract

Salmonella typhimuriumis a bacterium that causes digestive infections, gastroenteritis, and food poisoning, psickcaused byBacterial infection is a health problem in developing countries, including Indonesia. Green meniran (Phyllanthus niruri L.), is a herbaceous plant containing flavonoids, alkaloids, saponins, and tannins which have antibacterial activity which is expected to suppress the development of Salmonella sp. The aim of the study was to determine the ability of green meniran extract as an antibacterial to inhibit the growth of Salmonella typhimurium bacteria. The study used a completely randomized factorial design consisting of two factors, namely extract concentrations of 5%, 10%, 20%, 40%, 80%, equipped with a positive control (chloramphenicol) and negative control (aquades), as well as the age of the inoculum of the bacterial growth phase. Testing the antibacterial activity using the Kirby-Bauer disc diffusion method. The results showed that green meniran extract had antibacterial activity against the growth of Salmonella typhimurium bacteria. Concentrations of 5%, 10%, 20%, 40%, and 80% produced inhibition zone diameters of 6.7 mm, 8.5 mm, 9.9 mm, 12 mm, and 14.6 mm, respectively. The concentration of 80% green meniran extract is effective in inhibiting the growth of Salmonella typhimurium bacteria with an antibacterial effectiveness value of 58.95%.
Inducing An Axillary Bud of Dendrobium Red Emperor ‘Prince’ With An Addition of Bap in Vitro Septasari, Mistri; Mercuriani, Ixora Sartika
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 2 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i2.216

Abstract

The purpose of this study is to determine the effect of the addition of BAP and the position of nodus on the formation of axillary shoots and to know the optimum concentration of BAP for the induction of Dendrobium Red Emperor 'Prince' axial shoots. The research design used is a Complete Factorial Randomized Design consisting of one treatment, namely: concentration of growing regulatory substances (ZPT) BAP (0ppm, 1 ppm, 2 ppm, and 3 pmm), each treatment repeated 5 times. The explant of the nodus were taken from plants produced by previous in vitro culture having 5 nodi. The basic medium used is New Phalaenopsis (NP) + Coconut Water + 1 ppm 2.4-D). The growth of axillary bud growth is measured based on the time of the bud emergence, growth of crown, and that of root. Data obtained were then analyzed using ANOVA. If there was a significant difference, the analysis was continued with a test of DMRT with a significant level of 5%. The results showed the addition of concentrations of 1 ppm and 2 ppm BAP influenced the time of the bud emergence, growth of crown, and that of root. The optimum concentration of BAP to induce the orchid axillary shoots is 2 ppm.

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