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Bioeksperimen
Published by Universitas Muhammadiyah Surakarta
ISSN : 24601365     EISSN : 25272799     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Aim Bioeksperimen: Jurnal Penelitian Biologi is a peer-reviewed, open-access scientific journal dedicated to advancing knowledge across a broad spectrum of biological sciences through the publication of high-quality original research articles. The journal promotes rigorous experimental and exploratory research that advances fundamental and applied understanding in biological sciences. Bioeksperimen provides an international platform for researchers, academics, and practitioners to disseminate innovative empirical findings and scientifically robust investigations that contribute to the development of biological sciences at regional and global levels. The journal welcomes manuscripts that: 1. Present original experimental or exploratory research in biological sciences 2. Demonstrate strong methodological design and scientific rigor 3. Contribute novel data, findings, or biological insights 4. Address contemporary biological challenges and emerging scientific issues 5. Integrate laboratory, field, and computational approaches 6. Contribute meaningfully to the advancement of biological sciences All manuscripts must be original, unpublished, written in English, and must clearly demonstrate methodological soundness, data-driven analysis, and a significant contribution to biological sciences. Since March 2026, Bioeksperimen does not publish literature review articles, conceptual papers, or purely theoretical manuscripts. Focus The primary focus of Bioeksperimen: Jurnal Penelitian Biologi is on empirical biological research based on experimental, observational, or exploratory approaches. The journal prioritizes studies that: 1. Apply molecular, physiological, ecological, microbiological, or genetic techniques 2. Utilize omics-based and bioinformatics approaches to generate primary data 3. Conduct laboratory experiments, field investigations, or integrative biological exploration 4. Produce reproducible and evidence-based scientific findings Scope Bioeksperimen: Jurnal Penelitian Biologi publishes original research in the following areas: Main Topics: 1. Ecology and Environmental Sciences 2. Molecular Biology of Plants, Animals, and Microorganisms 3. Bioinformatics and Computational Biology 4. Omics-Based Studies (Genomics, Transcriptomics, Metagenomics, Proteomics, Metabolomics) 5. Basic and Applied Microbiology 6. Plant and Animal Physiology 7. Animal Behavior 8. Plant and Animal Systematics and Taxonomy 9. Genetics of Plants, Animals, and Microorganisms 10. Human Physiology
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 34 Documents
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Alfalfa (Medicago sativa L.) as a Promising Herbal Galactagogue for Dairy Cows: A Review Nasihin, Ahmad
Bioeksperimen: Jurnal Penelitian Biologi September 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.11779

Abstract

Milk production in dairy cows can be enhanced through herbal-based approaches like alfalfa (Medicago sativa L.), which is known for its richness in nutrients and bioactive compounds with the potential to act as an herbal galactagogue. This study reviews scientific publications from 2015 to 2025 in the Google Scholar database to explore the use of alfalfa and its benefits for dairy cow performance. The results indicate that alfalfa can increase milk production and quality, particularly in fat, protein, and lactose content. Phytoestrogen compounds such as isoflavones and coumestrol in alfalfa stimulate prolactin hormone production and promote udder tissue growth. Phenolic compounds help improve milk yield and quality, while alkaloids enhance the milk ejection reflex. Additionally, saponins serve as defaunation agents in the rumen, increase nitrogen utilization efficiency, and support metabolic balance, positively impacting overall cow health. However, high doses of alfalfa may lead to reproductive issues due to excessive estrogenic activity, such as anestrus and infertility. The effectiveness of alfalfa also depends on factors like material quality, dosage, the cow's physiological condition, and management practices. Therefore, alfalfa should be used carefully and in balanced amounts as a functional feed to maximize benefits and prevent physiological side effects in dairy cows.
Ethanolic Extract of Tamarindus indica Leaves Lowers Total Cholesterol, Triglycerides, and HDL but Without Affecting LDL in Hyperlipidemic Rats Susiloningsih, Tri; Christijanti, Wulan; Marianti, Aditya; WH, Nugrahaningsih
Bioeksperimen: Jurnal Penelitian Biologi September 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.12103

Abstract

Hyperlipidaemia is a condition of increased lipid levels, especially cholesterol, triglycerides, LDL and an abnormal decrease in HDL in the blood. Tamarind leaves contain flavonoid and quercetin compounds that have the ability as antihyperlipidemia agents. The purpose of this study was to examine the effect of tamarind leaf ethanol extract on the lipid profile of hyperlipidaemia rats. Samples of 16 male Wistar rats were divided into 4 groups; Control group and the tamarind leaf ethanol extract treatment group was divided into 3, namely P1 350 mg/kgBB, P2 500 mg/kgBB, and P3 650 mg/kgBB. All rats were given lard 3 mL/head/day for 2 weeks. In weeks 3 and 4, rats were given extract of Tamarindus indica leaves and 2 hours interval was given lard. The data obtained were statistically analysed using one-way ANOVA and the Least Significant Difference (LSD) test at the 95% confidence level. In this study, HDL levels decreased along with reductions in total cholesterol and triglycerides, but had no effect in reducing LDL levels. Reduced cholesterol synthesis limits substrate availability for HDL formation, resulting in proportional decreases in HDL-C. The conclusion of this study is that tamarind leaf ethanol extract has an effect in reducing lipid profile levels.
Diversity of Pollinator Insects in Anagro Garden, Bengkulu City, Indonesia Wisti, Lilis; Apriiarti, Mega Sari; Cahaya, Mariana Ade
Bioeksperimen: Jurnal Penelitian Biologi September 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.12305

Abstract

Pollinator insects are considered beneficial organisms due to their essential role in plant pollination, supporting both ecological stability and agricultural productivity. However, land-use change and pesticide application may reduce pollinator diversity, making it necessary to identify existing species and assess their diversity as indicators of ecosystem health. This study aimed to identify the species and assess the diversity of pollinator insects in Anagro Garden, Bengkulu City. Field research was conducted from January to March 2025 using an exploratory survey method combined with direct observation. Sampling was carried out across multiple points within the garden. The results showed that pollinator insects observed in this area belonged to four insect orders. The calculated Shannon-Wiener Diversity Index (H′) was 1.317, indicating a moderate level of species diversity. The Dominance Index (D) was 0.283, showing that no single order overwhelmingly dominated the assemblage. The Evenness Index (E) was 0.950, reflecting a high level of evenness and very uniform distribution of individuals among species. These findings suggest that although species abundances vary, the pollinator community remains balanced. The moderate diversity level indicates that the ecosystem quality in Anagro Garden is still fairly goodHowever, the study underscores the need for ongoing monitoring and habitat management to enhance pollinator diversity and maintain ecological resilience, particularly in semi-urban agroecosystems like those found in Bengkulu City.
The Enhancing Single Cell Protein Production of Saccharomyces cerevisiae Using Soybean Hull Waste with Dextrose Variations ., Musa'adah; Darniwa, Adisty Virakawugi; Kartika Sari, Dede
Bioeksperimen: Jurnal Penelitian Biologi September 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.12356

Abstract

Single Cell Protein (SCP) is a microprotein derived from the dry biomass of non-pathogenic microorganisms and offers a promising alternative protein source for both humans and animals. One of the advantages of SCP production is its potential to utilize organic waste as a growth medium, thereby contributing to environmental sustainability. Soybean hull waste has been identified as a nutrient-rich substrate capable of supporting the growth of various microorganisms used in SCP production. This study aimed to optimize the growth medium for S. cerevisiae by utilizing soybean hull waste supplemented with varying concentrations of dextrose, in order to determine the most effective dextrose level for SCP production. The experiment involved cultivating S. cerevisiae in filtrates of soybean hull waste supplemented with five different dextrose concentrations: 0% (FD0), 1% (FD1), 2% (FD2), 3% (FD3), and 4% (FD4), as well as a standard Yeast Potato Dextrose (YPD) medium used as a control. Fermentation was initiated with an inoculum concentration of 1.3×10⁶ CFU/mL across all treatments then incubated at 28–30°C for 72 hours. Biomass, moisture content and protein content of yield were measured at the end of the fermentation. The results showed the dextrose conc. significantly affected pH levels, biomass yield, moisture, and protein content of the final product. Higher dextrose conc. generally led to better yield. Among the treatments, FD4 (4% dextrose) produced the highest biomass (0.091 g/10 mL) and protein content (18.43%). These finding showed that soybean hull waste can serve as a sustainable growth medium for the S. cerevisiae-SCP production, with 4% dextrose supplementation yielding the most optimal results.
Sumberejo Community's Perspective on Non-Timber Forest Products to Support Sustainable Environment Sari, Cynthia Permata; Supriyadi; Purwanto; Rosariastuti, Retno; Nufus, Malihatun
Bioeksperimen: Jurnal Penelitian Biologi September 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.13643

Abstract

This study investigates how Non-Timber Forest Resources (NTFPs) contribute to community livelihoods and environmental sustainability. Through a combination of in-depth interviews and participatory observation, this study highlights the traditional knowledge and practices of the Sumberejo community in managing these resources. This research uses a qualitative approach that combines ethnobotanical surveys, in-depth interviews, and participatory observation with 50 participants, as well as data analysis using Participatory Rapid Appraisal (PRA). The results show that the majority of respondents perceive the potential of NFTPs in the area as very important (56.25% of village officials) and important (45.83% of villagers). Respondents also consider the natural geophysical potential for NFTPs in the area to be very important (68.75% of village officials) and important (50% of villagers). Furthermore, respondents also considered the natural biophysical potential for the NFTPs area to be very important (56.25% of village officials) and important (50% of villagers). The study underscores the potential for integrating NTFP management into broader conservation strategies to promote environmental stewardship and sustainable development. The community's commitment to balancing economic needs with environmental health offers valuable insights for policymakers and conservationists aiming to implement community-based forest management models. This research contributes to a growing body of knowledge on the importance of NTFPs in sustainable environmental governance, showcasing the Sumberejo community's role as stewards of their local ecosystems while maintaining cultural heritage and securing economic well being.
Antibacterial activity of miana (Coleus scutellarioides) against Streptococcus pyogenes and Streptococcus mutans Aprillia, Klara; Sianturi, Sister
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.9152

Abstract

Miana leaves (Coleus scutellarioides) have compounds that have the potential to be antibacterial. The bacteria Streptococcus pyogenes and Streptococcus mutans are a group of positive bacteria containing a lipid layer that is low in peptidoglycan and teichoic acid. This study aims to determine the antibacterial activity of Miana leaves against Streptococcus pyogenes and Streptococcus mutans because both types of infectious bacteria. This research used the disc diffusion method. The preparation involved the maceration method using 96% ethanol as a solvent for five days, followed by concentration using a water bath at 70°C to obtain a thick extract. Observations were made on the diameter of the inhibition zone visible in the clear zone around the disc. The average inhibition diameter by ethanol extract against S. pyogenes is 9.83 mm, 10.76 mm, 12.42 mm, 15.66 mm, and 28.56 mm, respectively at extract concentrations of 5, 10, 20, 40, and amoxicillin (positive control). The negative control 1% DMSO did not form an inhibition zone. The statistical analysis using One-Way ANOVA (SPSS 27) yielded a p-value of < 0.05, confirming a significant difference in antibacterial activity across all tested groups. This result demonstrates that Miana leaf ethanol extract at concentrations of 5%, 10%, 20%, and 40% effectively inhibits the growth of both S. pyogenes and S. mutans. Furthermore, the inhibition zones varied significantly between the extract concentrations and the control groups, proving that the extract’s efficacy is concentration dependent. These findings suggest that Miana leaf ethanol extract has potential as a natural antibacterial agent for developing alternative phytotherapeutic treatments against S. pyogenes and S. mutans infections.
Synergistic cultures for resilient mercury bioremediation in ASGM leveraging microbial interactions for sustainable pollutant removal Wibowo, Sekar; Purwanti, Ipung Fitri; Firdaus, Abiyyu Armijn Firman
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.11967

Abstract

The widespread use of mercury (Hg) in artisanal and small-scale gold mining (ASGM) necessitates effective bioremediation strategies. This study evaluated the mercury reduction capabilities of two bacterial strains, Bacillus subtilis and Pseudomonas aeruginosa, using real-world ASGM liquid waste. In a seven-day laboratory-scale experiment, we assessed the performance of single and mixed bacterial cultures under varying pH conditions. Our results show that B. subtilis was particularly effective, achieving a maximum Hg2+ removal efficiency of 90.07%. Critically, while the cell viability of single cultures declined significantly over the study period, mixed cultures maintained superior population stability, reaching 7.4 log CFU/mL on day 7, especially under alkaline conditions. This stability suggests a beneficial synergistic relationship between the two species. The enhanced long-term viability and robust detoxification mechanisms observed in the mixed culture system underscore the high potential of this approach for developing sustainable bioremediation solutions for the ASGM sector.
A Vegetation analysis of terestrial fern in kemuning tea garden in ngargoyoso village, Indonesia Yasmin, Hasna Aulia; Roziaty, Efri
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.12195

Abstract

Kemuning Tea Garden in Ngargoyoso Village is a beautiful area with many tourist destinations. Therefore, in addition to the activities of local people as tea farmers, many outsiders come as tourists. This area has abiotic factors that are suitable for the growth of various types of plants, one of which is ferns, especially terrestrial ferns. This study aims to determine the composition of terrestrial fern vegetation in the kemuning tea garden area. The method used was purposive sampling method, with 6 plots in 3 Stations with different heights: Station 1 (800 - 900 m asl), Station 2 (900 - 1000 m asl), and Station 3 (>1000 m asl). This study obtained 9 species of terrestrial ferns: Pityrogramma calomelanos, Adiantum raddianum, Pteris ensiformis, Pteris biaurita, Christella dentata, Nephrolepis exaltata, Dicranopteris linearis, Lycopodiella cernua, and Dryopteris dilatata; that belong to 3 families: Polypodiaceae, Gleincheniaceae, and Lycopodiaceae. The study results show that the type of Nephrolepis exaltata gets the highest INP value, which is 92,88 %. While the lowest INP index is obtained by Pityrogramma calomelanos, which is 9,52 %. The diversity index obtained by the Shannon-wiener formula is 1,85; which means that the diversity in Kemuning Tea Garden is moderate. These findings highlight the importance of adaptive vegetation management in tea plantation landscapes to maintain fern diversity while supporting sustainable agroecosystem functions.
In vitro antifungal activity of Mimosa pudica rhizosphere bacteria against Fusarium spp. Sriwulan, Sriwulan; Mustikaningrum, Dhina; Nurfitria, Nia
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.13509

Abstract

This study aims to explore rhizosphere bacteria from Mimosa pudica L. as a source of antagonistic bacteria against Fusarium spp. Rhizosphere soil samples were collected, isolated, morphologically characterized, and tested for antifungal activity in vitro against Fusarium spp. The percentage of inhibition (PI) was calculated after 7 days. Six bacterial isolates were obtained and tentatively identified as Bacillus, Cellulomonas, Arthrobacter, and Micrococcus species. All isolates exhibited antagonistic activity against Fusarium spp., with inhibition percentages ranging from 61.99% to 81.83%. Isolate PM2 (tentatively identified as Cellulomonas) demonstrated the strongest inhibition at 81.83%, followed by isolate PM6 (tentatively identified as Micrococcus) at 79.25%. These results confirm the potential of Mimosa pudica L. as a source of biocontrol agents and warrant further investigation into their molecular identification, mechanisms of action, and in vivo efficacy.
Integrating BSF larvae for the sustainable bioconversion of banana peels and sprout hulls waste Rizkawati, Vina; Andhini, Shabrina Lathiifah; Noer, Mohamad Isnin; Yudistira, Dwi Harya; Kurniati, Tri Handayani; Lisanti, Elsa; Komala, Ratna; Herlin, Weri
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.13637

Abstract

The management of organic waste presents a significant challenge in Indonesia, especially fruit and vegetable by-products. Black Soldier Fly (BSF, Hermetia illucens) larvae offer an effective solution due to their high potential for organic waste conversion. This study investigated the efficacy of BSF larvae in reducing and bioconverting organic waste composed of banana peels and sprout hulls, and examined the influence of EM4 fermentation on larval growth performance. Waste Reduction Index (WRI) and bioconversion rate (BCR) were calculated as percentages and analyzed descriptively, followed by the Kruskal–Wallis test. Furthermore, larval length and body weight were measured at 7, 11, 14, and 18 days of larval age and analyzed using Kruskal–Wallis with Dunn’s post-hoc test. The results showed that EM4–fermented substrates containing a 1:3 ratio of banana peels to sprout hulls showed the highest waste reduction and bioconversion potential among all mixture treatments (P < 0.05). Although larval length and weight were lower than control substrate, no significant difference in larval length was detected, while individual larval weight was significantly higher compared to other organic waste combinations (P < 0.001). In this study, EM4 fermentation enhanced lignocellulosic degradation, improving substrate digestibility for BSF larvae and optimizing bioconversion efficiency, thus supporting more sustainable organic waste management strategies.

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