Bioeksperimen
Aim Bioeksperimen: Jurnal Penelitian Biologi is a peer-reviewed, open-access scientific journal dedicated to advancing knowledge across a broad spectrum of biological sciences through the publication of high-quality original research articles. The journal promotes rigorous experimental and exploratory research that advances fundamental and applied understanding in biological sciences. Bioeksperimen provides an international platform for researchers, academics, and practitioners to disseminate innovative empirical findings and scientifically robust investigations that contribute to the development of biological sciences at regional and global levels. The journal welcomes manuscripts that: 1. Present original experimental or exploratory research in biological sciences 2. Demonstrate strong methodological design and scientific rigor 3. Contribute novel data, findings, or biological insights 4. Address contemporary biological challenges and emerging scientific issues 5. Integrate laboratory, field, and computational approaches 6. Contribute meaningfully to the advancement of biological sciences All manuscripts must be original, unpublished, written in English, and must clearly demonstrate methodological soundness, data-driven analysis, and a significant contribution to biological sciences. Since March 2026, Bioeksperimen does not publish literature review articles, conceptual papers, or purely theoretical manuscripts. Focus The primary focus of Bioeksperimen: Jurnal Penelitian Biologi is on empirical biological research based on experimental, observational, or exploratory approaches. The journal prioritizes studies that: 1. Apply molecular, physiological, ecological, microbiological, or genetic techniques 2. Utilize omics-based and bioinformatics approaches to generate primary data 3. Conduct laboratory experiments, field investigations, or integrative biological exploration 4. Produce reproducible and evidence-based scientific findings Scope Bioeksperimen: Jurnal Penelitian Biologi publishes original research in the following areas: Main Topics: 1. Ecology and Environmental Sciences 2. Molecular Biology of Plants, Animals, and Microorganisms 3. Bioinformatics and Computational Biology 4. Omics-Based Studies (Genomics, Transcriptomics, Metagenomics, Proteomics, Metabolomics) 5. Basic and Applied Microbiology 6. Plant and Animal Physiology 7. Animal Behavior 8. Plant and Animal Systematics and Taxonomy 9. Genetics of Plants, Animals, and Microorganisms 10. Human Physiology
Articles
34 Documents
Evidence-based non-residential waste analysis to support 3R strategies and food recovery hierarchy: a case study in Solok Selatan
Fauzi, Mhd.;
Aziz, Rizki;
Chyntia, Nanda;
Titani, Fena Retyo;
Afrianto, Andika Wahyu
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta
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DOI: 10.23917/bioeksperimen.v12i1.16030
The amount of waste generation that increases without being accompanied by good waste management will cause pollution and decrease the aesthetic value of the environment. Non-residential waste originating from non-residential activities is also one of the contributors to waste entering the landfill. This study aims to analyze non-residential waste generation and composition as an evidence-based reference for each source in implementing the 3R concept and the Food Recovery Hierarchy (FRH). The number of sampling sets was determined at a minimum of 10% of each type of facility following SNI 19-3964-1994. Sampling was carried out on eight consecutive days with 14 sampling points in Area 1, 14 in Area 2, and 11 in Area 3. The total non-residential waste generation was 46.79 m³/day (9.49 tons/day). Organic waste dominated the composition, accounting for 35–37% as food waste, followed by plastic (24–29%) and paper (14–20%). The high proportion of biodegradable and recyclable materials indicates significant potential for composting, recycling, and food recovery strategies at the source level. Implementing source-level 3R and FRH approaches could substantially reduce landfill dependency and extend landfill lifespan. This study addresses a knowledge gap by linking source-level non-residential waste characterization with the practical implementation of the 3R concept and the Food Recovery Hierarchy. This study provides quantitative evidence to guide localized waste minimization strategies in non-residential sectors.
Antagonistic activity of phyllosphere fungi isolated from medicinal plants against Colletotrichum sp. causing anthracnose in chilli (Capsicum annuum L.)
Puspita, Ayumi Rizci;
Alamsjah, Feskaharny;
Mildawati, Mildawati;
Agustien , Anthoni
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta
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DOI: 10.23917/bioeksperimen.v12i1.16072
Anthracnose disease caused by the fungus Colletotrichum sp. is one of the main problems in chili cultivation (Capsicum annuum L.) and can cause significant yield losses. The intensive use of chemical pesticides in controlling this disease has a negative impact on the environment and health. This study aimed to evaluate the antagonistic potential of phyllosphere fungi isolated from five medicinal plants, namely Phaleria macrocarpa, Curcuma domestica, Kleinhovia hospita, Hibiscus rosa-sinensis, and Morus macroura, against the pathogen Colletotrichum sp. Isolation was performed from leaf surfaces, and a total of 7 phyllosphere fungal isolates were tested using a double culture method with three replicates. The results showed that all isolates were able to inhibit the growth of Colletotrichum sp. through mechanisms of competition for space and nutrients as well as antibiosis, with inhibition levels ranging from high to very high. Among the tested isolates, Trichoderma sp. exhibited the highest inhibitory activity with an inhibition percentage of 92.78%, categorized as very high. Macroscopic and microscopic characterization supported the identification of several isolates belonging to the genera Trichoderma. This study concluded that phyllosphere ungi from medicinal plants have the potential as environmentally friendly biological control agents in combating anthracnose disease in chili plants.
Design and validation of PIP gene primer for quantitative PCR in Capsicum annuum using in silico and experimental approaches
Nurhafitri, Amanda;
Suwirmen
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta
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DOI: 10.23917/bioeksperimen.v12i1.16129
Capsicum annuum L. is an economically important horticultural crop whose productivity is strongly affected by drought stress. Plasma Membrane Intrinsic Protein (PIP), a member of the aquaporin gene family involved in water transport and osmotic regulation, represents a key target for drought stress studies, requiring highly specific and efficient primers for accurate gene expression analysis using quantitative PCR (qPCR). This study aimed to design and validate PIP gene primers for qPCR in C. annuum using integrated in silico and experimental approaches. Primer design was performed using NCBI Primer-BLAST based on the CaPIP reference sequence (XM_016711608.2), followed by in silico evaluation of primer specificity and secondary structure using Primer-BLAST and OligoAnalyzer. Nine primer pairs were initially generated and evaluated based on primer length, %GC, Tm, self3’ complementarity and amplicon size. Secondary structure analysis revealed strong self-dimer formation in pair 8, whereas pair 2 showed weak secondary structure within acceptable ΔG threshold (-9 kcal/mol). Experimental validation was conducted throught gradient PCR to optimize annealing temperature, followed by agarose gel 2%. Primer 2_CaPIP produced specific and clear amplification, with an optimal annealing temperature of 57.3oC. This study provides a validated CaPIP primer set suitable for qPCR-based gene expression analysis in C. annuum, supporting future molecular studies on drought stress tolerance.
Preliminary study of acute febrile response to crude LPS extract from Salmonella typhimurium in domestic chicken (Gallus gallus domesticus) AKY strain
Wijaya, Rizky Surya;
Lelono, Asmoro;
Oktarianti, Rike
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta
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DOI: 10.23917/bioeksperimen.v12i1.16139
Salmonella is a genus that commonly attacks the health of poultry, especially chickens. One type of poultry that is widely attacked by S. typhimurium is chickens. S. typhimurium infects the host with Lipopolysaccharides (LPS). This study aims to determine the innate immune response of domestic chickens to LPS-based vaccines S. typhimurium as the first step in vaccine development. The sample was in the form of female domestic chickens of the AKY strain which was divided into 3 groups, the control group was injected using 0.1ml of PBS (Phosphate Buffer Saline) treatment group 1 was injected with LPS with an adjuvant with a concentration of 0.5mg/kg as much as 0.1ml. Treatment group 2 was injected with LPS with an adjuvant with a concentration of 1mg/kg of 0.1ml. Injection treatment group using Incomplete Freund Adjuvant (IFA). The results obtained were that the treatment groups had an increase in temperature at the 1st hour to the highest in the 3rd hour with a temperature range of 42.5°C to 42.6°C, contrast to the control group which has a static temperature with a range of 41°C to 41.8°C. The comparison of chicken body temperature among treatment groups using a Liner Mixed Model revealed a significant difference (p < 0.001) between the treatment groups and the control group. These results indicate that subcutaneous LPS administration induces a controlled acute febrile response, which is consistent with involvement of innate inflammatory pathways.