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Bioeksperimen
Published by Universitas Muhammadiyah Surakarta
ISSN : 24601365     EISSN : 25272799     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Aim Bioeksperimen: Jurnal Penelitian Biologi is a peer-reviewed, open-access scientific journal dedicated to advancing knowledge across a broad spectrum of biological sciences through the publication of high-quality original research articles. The journal promotes rigorous experimental and exploratory research that advances fundamental and applied understanding in biological sciences. Bioeksperimen provides an international platform for researchers, academics, and practitioners to disseminate innovative empirical findings and scientifically robust investigations that contribute to the development of biological sciences at regional and global levels. The journal welcomes manuscripts that: 1. Present original experimental or exploratory research in biological sciences 2. Demonstrate strong methodological design and scientific rigor 3. Contribute novel data, findings, or biological insights 4. Address contemporary biological challenges and emerging scientific issues 5. Integrate laboratory, field, and computational approaches 6. Contribute meaningfully to the advancement of biological sciences All manuscripts must be original, unpublished, written in English, and must clearly demonstrate methodological soundness, data-driven analysis, and a significant contribution to biological sciences. Since March 2026, Bioeksperimen does not publish literature review articles, conceptual papers, or purely theoretical manuscripts. Focus The primary focus of Bioeksperimen: Jurnal Penelitian Biologi is on empirical biological research based on experimental, observational, or exploratory approaches. The journal prioritizes studies that: 1. Apply molecular, physiological, ecological, microbiological, or genetic techniques 2. Utilize omics-based and bioinformatics approaches to generate primary data 3. Conduct laboratory experiments, field investigations, or integrative biological exploration 4. Produce reproducible and evidence-based scientific findings Scope Bioeksperimen: Jurnal Penelitian Biologi publishes original research in the following areas: Main Topics: 1. Ecology and Environmental Sciences 2. Molecular Biology of Plants, Animals, and Microorganisms 3. Bioinformatics and Computational Biology 4. Omics-Based Studies (Genomics, Transcriptomics, Metagenomics, Proteomics, Metabolomics) 5. Basic and Applied Microbiology 6. Plant and Animal Physiology 7. Animal Behavior 8. Plant and Animal Systematics and Taxonomy 9. Genetics of Plants, Animals, and Microorganisms 10. Human Physiology
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 14 Documents
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Microbiological Quality of Packaged Tea Beverages Using the 3M Petrifilm Rapid E. coli/Coliform Count Plate Eka Kurniati; Fandi Tri Fajar Cahyo; Fitria Romadona
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 1 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i1.9603

Abstract

Tea is a complementary drink which is currently being served in variety ways and can be found in many places. The form of packaging is also very diverse. Glass bottles, plastic bottles, metal cans, paper cups, and plastic cups are just few of the many different forms of packaging that are available. The Indonesian National Standards Agency has established several requirements that bottled drinking water must fulfill in order to be legally sold in the country; one of these requirements is that it cannot contain any bacteria that could potentially cause disease. However, the reality is that within the community there are still packaged drinks that are not safe for human consumption. This study tested the quality of bottled tea supplied in a university canteen using 10 brands in varying packaging. 3M Petrifilm Rapid E. coli/Coliform Count Plate is utilized. The test results showed that 10 brands of tea packaging that were sold negatively did not contain E.coli and Coliform, which could be seen by the lack of gas bubbles and discoloration on the petrifilm media. This means that all packaged beverage samples sold are safe to drink.
Analysis Of Ichthyofauna Of Situ Bagendit and Water Quality Post-Revitalization: Water quality data, Fish Diversity and Abundance Data Abdul Faqih Fajar Sidik Sidik; Hertien Koosbandiah Surtikanti; Hernawati Hernawati; Haryono Haryono
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 1 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i1.10057

Abstract

Situ Bagendit is one of the natural lakes in Garut Regency that has experienced a decline in environmental quality, so revitalization was carried out to improve the ecosystem and increase tourist attractions. Sampling at three observation stations based on water characteristics. The parameters observed include biotic factors (fish diversity) and abiotic factors (physical and chemical parameters of water. The Shannon-Wiener diversity index is used to assess the stability of the fish community, while water quality is analyzed based on water quality standards according to PP No. 22 of 2021 and lake trophic classification. The results of the study showed that water quality data that did not meet the threshold according to PP No. 21 of 2021 were water clarity (75-136 cm), chemical oxygen demand (COD) (21-31 mg/L), total nitrogen (1.22-1.59) and conductivity (506-588 μs/cm). Fish diversity is at a moderate level with an index of 1.22. The abundance value ranges from 4-55% with the highest abundance value being the hemichromis elongatus species (zebra tilapia), but there is no dominant species. A fish species found as an indicator of pollution is the janitor fish (pterygoplichthys pardalis) due to its high adaptability to polluted water. While several water quality parameters have not fully met class II quality standards, especially in terms of brightness, COD, total nitrogen, and conductivity. Ecosystem conditions of Situ Bagendit still requires sustainable management to maintain water quality and biodiversity.
Pectin Extraction from Various Sources: A Literature Review Vira Triyatna; Fetriyuna Fetriyuna; Miftakhur Rohmah
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 1 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i1.10066

Abstract

Plant cell walls include a complex polymer called pectin, which combines methoxyl with galacturonic acid to produce a gel. Applications for pectin are found in both the food and non-food industries. It is utilized as an edible coating, gel-forming, texture enhancer, stability enhancer, and appearance enhancer in the food industry. Pectin can be combined with other ingredients to make non-food products. For example, pectin, chitosan, carboxymethyl cellulose (CMC), and alginate can be used to make biocomposites for wound dressings. Other sources of pectin include aloe vera, biosorbent, banana peel, corn starch, albedo watermelon, nutmeg, pumpkin, and dragon fruit skin. The properties of pectin are generally determined by its yield, equivalent weight, methoxyl content, galacturonic acid content, and degree of esterification. The two most common methods used to extract pectin are chemical and mechanical. Typically, chemical processes use solvents and mechanical techniques to combine liquid and solid components.
Behavioral Interaction of Male and Female Canaries (Serinus canaria) During the Reproduction Period Anwari Adi Nugroho; Citra Aisyah Nur'Aini; Asma' Asy-syifaiyah; Parigi Akhiri Septianingrum
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 1 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i1.10067

Abstract

The study aimed to describe the interaction between male and female bird canary (Serinus canaria ) during reproduction. Study This uses the ad libitum sampling method, namely observing activity act in demand bird canary male and female (Serinus canaria) during mating. Study This was done 6 days on Sunday, the first (2 days) introduction bird canary, and Sunday, the second and third (4 days) matchmaking bird canary. Research results show various Acts in demand reproduction of bird canaries during mating. Observation results show that bird canary males use tweets and appearances and physiques for interesting attention female, temporary females choose partners based on tweets male and mark fitness other. After the introduction process, birds canary mated males and females​ collaborate in the guard nest and raise children. The study still has limitations on the relative sample​ , as it only uses two pairs of bird walnuts and the duration of the observations​ is limited to three Sundays. This matter does not fully represent all the variations in existing behavior​ in the population of bird canaries. Apart from that, the factors influencing the environment and conditions and other specific aspects of on-site research must also be considered when interpreting results.
DREB1 Gene in Regulating Drought Tolerance in Cereal Plants Ajeng Klarica Todingan; Agista Aprilian Pailang; Yusminah Hala
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 1 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i1.10172

Abstract

This study aims to explain the role of the DREB1 gene in increasing drought tolerance in plants with an emphasis on recent advances in genetic engineering. In this research, the method used is a literature review by collecting library data, reading and taking notes, and critically managing research data related to the DREB1 gene. In this research, data analysis starts by examining the results from studies that are most relevant to the research problem. The results of the literature review obtained indicate that the DREB1 gene is a transcription factor that plays a role in regulating a number of other genes related to drought characteristics. The DREB genes participate in hormone signalling pathways and regulate proteins that interact with downstream genes to improve plant stress tolerance. When there is a drought, seedlings that have the DREB gene inserted have a survival rate of 36%, while plants without the DREB gene all die. These results indicate that the DREB1 gene is a positive regulatory gene for heat tolerance in plants. In this case, the transgenic lines keep their stomata partially closed, which helps maintain a minimum transpiration rate to cool the canopy and reduce the temperature of both the canopy and leaves. We achieve this by coordinating the control of stomata, minimising water loss, and preserving cell turgor, all of which prevent the plant from dying.
Antibacterial Activity of Endophytic Bacteria from Butterfly Pea (Clitoria ternatea L.) Root against Staphylococcus aureus and Escherichia coli Chersa Polandos; Any Fitriani
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 1 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i1.10346

Abstract

Indonesia is rich in biodiversity, with many medicinal plants such as Clitoria ternatea (butterfly pea) known for antibacterial properties. This study aimed to evaluate the antibacterial activity of ethyl acetate extracts from endophytic bacteria isolated from C. ternatea roots, with potential application in developing natural antibacterial agents. The bacteria were identified as belonging to the genera Bacillus, Pseudomonas, and two strains of Azomonas (A and B). Extracts were tested against Staphylococcus aureus and Escherichia coli using Disk Diffusion Assay (DDA), Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and Time-Kill assays. Results showed Pseudomonas extract at 40 mg/ml had the largest inhibition zone against S. aureus (6.75 ± 0.353 mm), while Azomonas A was most effective against E. coli (3.187 ± 0.618 mm). The lowest MIC against S. aureus was 2.5 mg/ml from Azomonas B, with MBCs of 20 mg/ml for Pseudomonas and Azomonas B. Against E. coli, Azomonas A showed MIC and MBC of 10 mg/ml and 40 mg/ml, respectively. Time-Kill assays confirmed bacterial reductions at MIC and higher concentrations. These findings suggest C. ternatea’s endophytic bacteria extracts have promising antibacterial potential for sustainable medicinal use.
Karamunting Leaf Extract (Rhodomyrtus tomentosa (Aiton) Hassk.) as antibiofilm on Escherichia coli Tia Sabrina; Muhammad Syafi’i Mansur; Masayu Farah Diba; Rizki Andini Nawawi; Nia Savitri Tamzil
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 1 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i1.10815

Abstract

Escherichia coli is a bacterium that causes many serious infections such as digestive tract, urinary tract and bloodstream infections. One of the main challenges in treating this bacterial infection is the formation of biofilm, which increases bacterial resistance to antibiotics. As an alternative to overcome antibiotic resistance, karamunting has been identified as having antibacterial potential against Gram-negative bacteria, including E. coli. The active compounds in these leaves have been shown to inhibit bacterial growth and biofilm formation. This research describes the activity of karamunting leaf extract against the anti-biofilm E. coli. The thick karamunting leaf extract was then made into three variations of concentration, namely 125µg/mL, 250µg/mL and 500µg/mL. The parameter measured for the eradication test is the thickness of the biofilm which is read using the Elisa Reader 590nm. In this study, the eradication percentages for the three concentrations were obtained -92.15% at a concentration of 125 µg/mL, -187.24% at a concentration of 250 µg/mL and 52.78% at a concentration of 500 µg/mL. Karamunting leaf extract has activity as an antibiofilm against E. coli bacteria. Karamunting leaf extract with a concentration of 500 µg/mL is the Minimum Inhibitory Concentration (MIC) which is effective as an antibiofilm on E. coli.
Impact of Meat Processing on DNA Quantity, Purity, and Integrity in Commercial Pork Products Assessed by TaqMan qPCR Ernawati Puji Rahayu; Abinawanto
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 2 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.11031

Abstract

TaqMan qPCR analysis for detecting adulteration in processed meat products requires DNA with a high quantity, purity, and integrity. This study aims to evaluate the quantity, purity, and DNA integrity of various commercial pork products focusing on their ability to amplify the Cyt b gene. Fresh pork was used as a positive control, while five processed products - jerky, floss, meatball, canned corned, and smoked pork - were extracted for analysis. The DNA concentration and purity then measured, visualized using gel electrophoresis, and subjected to qPCR amplification. Statistical analysis of DNA concentration, purity, and ct value was carried out using one-way ANOVA followed by the Tukey post hoc test. The results showed significant differences in concentration, purity, and Ct values between all samples and positive controls (P˂0.05). Meanwhile, the Tukey test showed that all samples and fresh pork concentration differed significantly (P˃0.05) except between canned corned and fresh pork (P˂0.05). The purity was significantly different between the positive controls with pork meatball and smoked pork (P˂0.05), while the Ct​ value of all samples and positive controls were significantly different (P˂0.05) except for fresh pork and jerky (P˃0.05). All the commercially processed products experienced DNA fragmentation. Meat processing affects DNA concentration, purity, genomic integrity, and the amplification ability of qPCR.
Isolation, Characterization, and Identification of Biosurfactant-Production Bacteria from Used Cooking Oil Waste Fisky Aditya Pratama; Pramesti Dewi; Ibnul Mubarok; Dewi Mustikaningtyas
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 2 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.11115

Abstract

Waste cooking oil constitutes a significant environmental concern in Indonesia. It has the potential to be utilized as a substrate for biosurfactant-producing bacteria. Biosurfactants are amphiphilic compounds produced by microorganisms, containing both hydrophilic and hydrophobic components that enable them to dissolve in both water and fats, as well as to reduce surface tension. This study aims to isolate, characterize, and identify biosurfactant-producing bacteria from waste cooking oil. Isolation proccess was conducted using Mineral Salt Medium enriched with cooking oil as an inducer and Carboxy Methyl Cellulose as a carbon source and emulsifier. Two isolates were successfully obtained and purified. Their biosurfactant-producing ability was evaluated through drop collapse test and oil spreading test. One isolate demonstrated superior performance, showing positive activity in both tests, including a 0.7 cm oil spreading zone. Identification involved macroscopic observation, Gram staining, endospore staining, and a catalase test. The isolate exhibited characteristics of a Gram-positive, rod-shaped, spore-forming, catalase-positive bacterium with small, circular, milky-white colonies and a smooth surface. This identification is non-molecular and based on a phenotypic approach according to Bergey’s Manual for preliminary bacterial classification. Based on these features, the isolate is presumed to belong to the genus Bacillus, known for its robust biosurfactant production. The findings suggest that waste cooking oil can be a promising source of indigenous biosurfactant-producing bacteria, contributing to both microbial conservation and potential development of eco-friendly biotechnological products.
Alfalfa (Medicago sativa L.) as a Promising Herbal Galactagogue for Dairy Cows: A Review Ahmad Nasihin
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 2 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.11779

Abstract

Milk production in dairy cows can be enhanced through herbal-based approaches like alfalfa (Medicago sativa L.), which is known for its richness in nutrients and bioactive compounds with the potential to act as an herbal galactagogue. This study reviews scientific publications from 2015 to 2025 in the Google Scholar database to explore the use of alfalfa and its benefits for dairy cow performance. The results indicate that alfalfa can increase milk production and quality, particularly in fat, protein, and lactose content. Phytoestrogen compounds such as isoflavones and coumestrol in alfalfa stimulate prolactin hormone production and promote udder tissue growth. Phenolic compounds help improve milk yield and quality, while alkaloids enhance the milk ejection reflex. Additionally, saponins serve as defaunation agents in the rumen, increase nitrogen utilization efficiency, and support metabolic balance, positively impacting overall cow health. However, high doses of alfalfa may lead to reproductive issues due to excessive estrogenic activity, such as anestrus and infertility. The effectiveness of alfalfa also depends on factors like material quality, dosage, the cow's physiological condition, and management practices. Therefore, alfalfa should be used carefully and in balanced amounts as a functional feed to maximize benefits and prevent physiological side effects in dairy cows.

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