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Ahmad Shafwan S. Pulungan
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Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan Jl. Willem Iskandar Psr V Medan Estate, Sumatera Utara
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JBIO: jurnal biosains (the journal of biosciences)
Published by Universitas Negeri Medan
ISSN : 24431230     EISSN : 24606804     DOI : https://doi.org/10.24114/jbio.v6i1
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Jurnal Biosains (JBIO) features works of exceptional significance, originality, and relevance in all areas of biological science, from molecules to ecosystems, (ie genetic, microbiology, ecology, biosystematic, biostatistic) including works at the interface of other disciplines, such as chemistry, medicine,physic and mathematics. We also welcome data-driven meta-research articles that evaluate and aim to improve the standards of research in the life sciences and beyond. Our audience is the international scientific community as well as educators, policy makers, patient advocacy groups, and interested members of the public around the world.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 269 Documents
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Identifying Sibutramine in Slimming Herbs Sold Online Rina Kurniaty; Fachruniza, Yunda; Rahmayani, Nabila
JURNAL BIOSAINS Vol. 11 No. 2 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i2.66791

Abstract

Obesity is defined as the presence of abnormal amounts of body fat and is a risk factor for diabetes and can increase the risk of hernias, varices and arthroses in the knees and feet. Consumption of slimming herbs is widely used to reduce this obesity. Slimming herbal products in their ingredients or compositions contain simplisia derived from medicinal plants and have pharmacological effects. According to the Minister of Health Regulation No. 007 of 2012 concerning the registration of traditional medicines, herbs circulating in the community must meet various requirements, one of which is that they must not contain drug chemicals, narcotics or psychotropic substances and other materials that based on health considerations and research can endanger health. Sibutramine is a weight loss drug that is often used, belongs to the class of hard drugs and can only be used based on a doctor's prescription because it can increase blood pressure. The identification of sibutramine in this study was carried out qualitatively using thin layer chromatography (TLC) method, to determine the presence or absence of the addition of drug chemicals in slimming herbs sold online. The mobile phase used was methanol (p): chloroform (p) (4:1) solvent with silica GF 254 stationary phase. The results of the study showed that two of the five slimming herbs sold online contained sibutramine with the Rf value of herbal medicine A = 0.86 and herbal medicine B = 0.91.
The Effectiveness of Aloe Vera Extract as A Natural Ingredient in Reducing Hair Loss nurmagrianty, khandika cece; Agustin , Eny Widhia
JURNAL BIOSAINS Vol. 11 No. 2 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i2.67996

Abstract

Hair loss is a common condition affecting many individuals, particularly teenagers and women who wear the hijab. Aloe vera contains various bioactive compounds, including vitamins, proteolytic enzymes, and amino acids, which are known to nourish the scalp and stimulate hair growth. This study aims to evaluate the effectiveness of Aloe vera extract in different types of hair care formulations, such as shampoos, hair tonics, serums, and pomades, for treating hair loss. A qualitative descriptive literature review was conducted by analyzing 30 relevant national and international scientific articles published between 2016 and 2025. The findings indicate that Aloe vera has the potential to reduce hair loss, promote hair growth, and support scalp health. Furthermore, the combination of Aloe vera with other herbal ingredients, such as candlenut, celery, and lime, may enhance its efficacy. Products based on Aloe vera are also reported to have good physical stability, be safe for use, and be compatible with various scalp types. The study concludes that Aloe vera is a promising natural ingredient for the development of safe, effective, and sustainable herbal hair care products.
Optimization and Evaluation of Noni Leaf Skin Softening Lotion Preparation (Morinda Citrifolia L.) barus, bunga rimta; Ilsanna Surbakti, Christica; Salman, Salman
JURNAL BIOSAINS Vol. 11 No. 2 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i2.68012

Abstract

The premature aging process is usually characterized by visible wrinkles or wrinkles on the face, dry and rough skin, age spots and decreased skin elasticity. Noni leaves (Morinda citrifolia L.) are one type of herbal plant that can potentially act as an antioxidant. The purpose of this study was to see Optimizing the Formulation of Lotion Preparations and Evaluating the Physical Properties of Lotion Preparations. Noni leaves were macerated with 96% ethanol solvent and then rotary with an evaporator. Hand and body lotion preparations were made by adding ethanol extract of noni leaves with several concentrations of 2.5% (F1), 5% (F2), and 7.5% (F3) to the base of hand and body lotion. As a blank (F0) the base of hand and body lotion without ethanol extract of noni leaves. Testing on the preparation was an evaluation of stability for 4 weeks at room temperature (odor, color, pH, emulsion type, homogeneity), irritation test and anti- aging skin activity test using a skin analyzer on hand skin. The parameters measured included water content, pore size, number of blemishes and number of wrinkles. Treatment was carried out using hand and body lotion every day for 4 weeks. Ethanol extract concentrations of 2.5%, 5% and 7.5% were homogeneous preparations with an oil-in-water emulsion type, pH 6.3-6.8, stable in storage for 4 weeks, and did not irritate the skin. Anti- aging skin hand and body lotion preparations containing ethanol extract of noni leaves can increase water content, reduce pore size, reduce the number of blemishes, reduce the number of wrinkles on the skin of the back of the hand and are stable in storage for 4 weeks. Hand and body lotion preparations with ethanol extract at a concentration of 7.5% had better skin antiaging activity compared to concentrations of 2.5%, 5% and blank.
Antioxidant Activity Test of Extract And Fractions of Red Guava (Psidium guajava L.) Peel and Fruit Monica; Rohmana, Vivin Marwiyati; Pratama, Kharisma Jayak
JURNAL BIOSAINS Vol. 11 No. 2 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i2.68054

Abstract

Red guava (Psidium guajava L.) contains natural antioxidants that help neutralize free radicals originating from radiation, pollution, and harmful chemicals. This study aimed to identify secondary metabolites and determine the IC₅₀ values of ethanol extract and various fractions of red guava peel and fruit. Four solvents were used ethanol extract, ethyl acetate fraction, n-hexane fraction, and water fraction. Each sample was tested at concentrations of 20, 40, 60, 80, and 100 ppm. Vitamin C, at concentrations of 2, 4, 6, 8 and 10 ppm, served as a positive control. Antioxidant activity was evaluated using the DPPH method with a UV-Vis spectrophotometer at a wavelength of 516 nm and an incubation time of 30 minutes. Phytochemical screening showed that all extracts contained alkaloids, flavonoids, and tannins. The IC₅₀ values obtained were: ethanol extract peel 62,4719 ppm, ethanol extract fruit 44,0035 ppm, ethyl acetate fraction peel 56,7955 ppm, ethyl acetate fraction fruit 46,4394 ppm, n-hexane fraction peel 113,9311 ppm, n-hexane fraction fruit 114,4767 ppm, water fraction peel 104,0284 ppm, and water fraction fruit 76,5801 ppm.
IDENTIFICATION AND PHYTOCHEMICAL TESTS OF FUNGI MUSHROOMS AND HONEY SPECIALLY BANGKA BELITUNG Purba, Eva Dewi Rosmawati; Djuria, Rachmawati Felani; Lingga, Rahmad
JURNAL BIOSAINS Vol. 10 No. 1 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i1.31428

Abstract

Pelawan Bitter Honey and Pelawan Mushroom are typical Bangka biodiversity found in Central Bangka Regency. The Pelawan mushroom grows in Bangka Belitung Islands Province and generally used as a food ingredient and has the potential to be developed as a source of natural immunomodulator agent. Phenolic compounds are known to have immunomodulatory activities that can improve the immune system. In addition, based on preliminary study, honey was also used by local people to strengthen the immune system. The purpose of this study was to identify the content of active compounds from Pelawan honey and Pelawan mushrooms. This research was an experimental study with several stages of testing including phytochemical screening, Thin Layer Chromatography and Extract Standardization tests. The results showed that based on phytochemical test of Pelawan honey contained flavonoids and alkaloids, while based on the Thin Layer Chromatography (TLC) test contained flavonoids and alkaloids. Phytochemical test of Pelawan mushroom showed the existence of Phenolic and Alkaloids, while based on TLC test containing flavonoids with an average Rf value of 0.56.  The standardization of Pelawan mushroom extract produced a total ash content of 28%, drying shrinkage of 40.88 g /mL, specific gravity 0.67 g/mL and water soluble extract content of 59.01% and ethanol soluble extract content of 41.12%. From these results, it can be assumed that the pelawan honey and pelawan mushroom have the potential to be developed as immunomodulatory agent.
ZINGIBERACEAE ETNOBOTANY AS A TRADITIONAL TREATMENT INGREDIENT FOR THE MANDAILING TRIBE IN VILLAGES AROUND THE BATANG GADIS NATIONAL PARK AREA, MANDAILING REGENCY NATAL Sakinah, Indah; Nasution, Aswarina
JURNAL BIOSAINS Vol. 9 No. 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.39061

Abstract

ABSTRACKThis study aims to uncover and document belief systems, local knowledge systems, practice of using plants and culturally important values or ICS of plants of the Zingiberaceae family which are used as ingredients for traditional medicine by the Mandailing Tribe in villages around the Batang Gadis National Park area, Mandailing Natal Regency. The research location is in 2 villages around the Batang Gadis National Park, Mandailing Natal Regency. The research method is qualitative and quantitative. Data was collected through semi-structured interviews, participatory observation, documentation, focus group discussions, collection of plant samples and making herbarium. The results showed that there were 10 species of plants from the Zingiberaceae family used as traditional medicine by the Mandailing Tribe. The most widely used species is turmeric or hunik ( Curcuma dometica Val.). Turmeric is believed to cure 8 kinds of diseases. The rhizome organ is the organ that is most widely used with a percentage of 75%. The practice of using plants by the Mandailing Tribe with the most processing method is boiling as many as 10 plant species with a percentage of 35.71% and the most using method by drinking as many as 10 plant species with a percentage of 62.5%. Plants with a very high category of cultural importance or ICS are found in turmeric or hunik ( Curcuma dometica Val.) with an ICS value of 150, ginger or pegeh ( Zingiber officinale Roscoe. ) and red ginger or pegeh narara (Zingiber officinale var. Rubrum) with an ICS value of 120. Keywords: Traditional Medicine, Belief system, Local knowledge, Utilization practice, ICS
BACTERIAL OBSERVATION WITH GRAM STAIN ON PURE CULTURE OF BACTERIA IN URINE Situmorang, Rahel; Syahirani, Zilan; Simanjuntak, Andreas
JURNAL BIOSAINS Vol. 10 No. 1 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i1.40183

Abstract

Bacteria are one of the microscopic organisms contained in the urine, and the presence of bacteria in the urine we call bacteriuria. Gram painting examination of urine with a microscope is one of the good examination methods to detect the presence of bacteriuria. This study aims to identify gram-positive bacteria and gram-negative bacteria in urine using the Gram staining method. The research method used is the experimental method. This research is in the laboratory by giving treatment to the object of study.  Sampling is urine, taken from the urine of one of the students of Medan State University. The results of the culture process are known that the urine sample (U4) has a coccus-shaped cell shape with a red cell color which indicates that the bacterial cells in this urine are included in the gram-negative type of bacteria. In gram-negative bacteria, the cell wall structure consists of three layers (the inner layer is peptidoglycan, one layer is murein base frame and the outer layer is lipopolysaccharide and lipoprotein), there is no teichoic acid, lysozyme softens the cell wall (detergents disorganize that wall by damaging the lipid layer), the cell wall is thin which is about 10-15 nm, less susceptible to penicillin, and not so absorbent of basic dyes but with safranin dyes (red) very absorb.
POTENTIAL ANALYSIS OF THE BIOLARVACIDAL PLANT ON Aedes aegypti FROM THE COMMUNITY YARD OF GUNUNGPUYUH SUB-DISTRICT, SUKABUMI CITY Munadiah, Hilda; Saraesa, Muhammad Nur Azzura; Ratnasari, Jujun
JURNAL BIOSAINS Vol. 10 No. 1 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i1.40339

Abstract

Dengue hemorrhagic fever (DHF) is an infectious disease caused by the dengue virus and transmitted through the bite of the Aedes sp. mosquito. This disease is one of the main problems in Indonesia. Sukabumi is one of the cities in West Java that has the highest case di DHF. Chemical larvicides were used to control the Aedes aegypti mosquito. But its cause resistance in mosquito larva, so the seek for alternatives larvicides from natural and environmentally friendly are sought. Many studies had carried out on alternatives larvicides derived from natural product (biolarvicides). Natural larvicides have one advantage, that they do not cause resistance in mosquitoes. Several studies on biolarvicides are summarized to obtain information on several plants planted by the community in Gunungpuyuh District, Sukabumi City, which have the potential as biolarvicides. Of the 343 species of plants planted by the community, seven species of plants have high effectiveness with an LC (Median Lethal Concentration) LC50 value of <750 ppm. Lemongrass, cucumber, soursop, and kaffir lime were plants that potencial as biolarvicides compared to the others with LC50 values of 1.553 ppm, 189.261 ppm, 279.882 ppm, and 603 ppm. This plant is easy to cultivate and can be used as a biolarvicides by the community.
ANALYSIS OF THE DIVERSITY OF UNDERSTORY SPECIES AT THE GREENHOUSE DEPARTMENT OF BIOLOGY, UIN ALAUDDIN MAKASSAR Rustam, Aswar; Hamzah, Pratiwi
JURNAL BIOSAINS Vol. 10 No. 1 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i1.40387

Abstract

Undergrowth is one of the constituents of a forest vegetation ecosystem. Over time, the condition of the land that has experienced succession will be overgrown by pioneer species and become understory. This research is to determine the distribution of understory species at the post-land clearing location at the greenhouse construction site, Department of Biology, UIN Alauddin Makassar. The variables of this research are diversity, evenness, dominance, and community similarity of understory plants that live in that location. Based on observations, the species distribution at the study site reached 35 species in 3 observation points. This condition indicates that the understory species in the research area are abundant and dominated by L. Nummularia, S. obtusifolia, and C. dactylon. The community similarity index shows a figure of 12%, which means the categories are not the same. The species diversity index shows that the three observation plots are moderate. Regarding the evenness, only S. nodiflora had a moderate evenness (0.6 ≤ e ≤ 0.4). Meanwhile, species with low evenness (e < 0.4) were C. dactylon, S. tora, and S. obtusifolia.
Effects of grey mangrove leaf extract (Avicennia marina) on the growth of Staphylococcus aureus Priambodo, Bima; Budiarti, Retno; Rahadianto, Rahadianto; Nefertiti, Eva
JURNAL BIOSAINS Vol. 10 No. 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.43222

Abstract

The emergence and spread of methicillin-resistant Staphylococcus aureus in recent decades complicates the antibiotic therapy. The grey mangrove (Avicennia marina) has flavonoid, terpenoid, saponin, phenolic, tannin, and alkaloid chemical compounds that have antimicrobial activity, thus potentially inhibiting Staphylococcus aureus. This study aims to examine the effects of the grey mangrove (Avicennia marina) leaf extracts on the growth of Staphylococcus aureus. This research uses laboratory experimental design with disc diffusion method to test the inhibition of Staphylococcus aureus bacteria growth in Mueller-Hinton medium. There were 6 treatment groups, namely negative control of 0.2% DMSO,  positive control of amoxicillin 30 µg, Avicennia marina leaf extract at concentration of 25%, 50%, 75%, and 100%. The results of this research showed that Avicennia marina leaf extract produces inhibition zone of 7.06 mm, 8.51 mm, 10.07 mm, 13.29 mm at concentration of 25%, 50%, 75%, and 100%. Meanwhile, positive control produces inhibiton zone of 23.65 mm, and negative control has no inhibition zone. The statistical tests using one-way ANOVA resulted in a significance value of less than 0.05 (p<α). The conclusion of this study showed that the grey mangrove (Avicennia marina) leaf extract at 100% concentration is the most effective treatment group in inhibiting the growth of Staphylococcus aureus, although the inhibition was not much better when compared to the inhibition of the positive control group.

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