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Ahmad Shafwan S. Pulungan
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Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan Jl. Willem Iskandar Psr V Medan Estate, Sumatera Utara
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JBIO: jurnal biosains (the journal of biosciences)
Published by Universitas Negeri Medan
ISSN : 24431230     EISSN : 24606804     DOI : https://doi.org/10.24114/jbio.v6i1
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Jurnal Biosains (JBIO) features works of exceptional significance, originality, and relevance in all areas of biological science, from molecules to ecosystems, (ie genetic, microbiology, ecology, biosystematic, biostatistic) including works at the interface of other disciplines, such as chemistry, medicine,physic and mathematics. We also welcome data-driven meta-research articles that evaluate and aim to improve the standards of research in the life sciences and beyond. Our audience is the international scientific community as well as educators, policy makers, patient advocacy groups, and interested members of the public around the world.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 269 Documents
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ANALYSIS OF THE DIVERSITY OF UNDERSTORY SPECIES AT THE GREENHOUSE DEPARTMENT OF BIOLOGY, UIN ALAUDDIN MAKASSAR Aswar Rustam; Pratiwi Hamzah
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 1 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i1.40387

Abstract

Undergrowth is one of the constituents of a forest vegetation ecosystem. Over time, the condition of the land that has experienced succession will be overgrown by pioneer species and become understory. This research is to determine the distribution of understory species at the post-land clearing location at the greenhouse construction site, Department of Biology, UIN Alauddin Makassar. The variables of this research are diversity, evenness, dominance, and community similarity of understory plants that live in that location. Based on observations, the species distribution at the study site reached 35 species in 3 observation points. This condition indicates that the understory species in the research area are abundant and dominated by L. Nummularia, S. obtusifolia, and C. dactylon. The community similarity index shows a figure of 12%, which means the categories are not the same. The species diversity index shows that the three observation plots are moderate. Regarding the evenness, only S. nodiflora had a moderate evenness (0.6 ≤ e ≤ 0.4). Meanwhile, species with low evenness (e < 0.4) were C. dactylon, S. tora, and S. obtusifolia.
BIOACTIVITY OF FRAGRANT PANDAN AND WULUH STARFRUIT COMBINATION LEAF POWDERS AGAINST THE MORTALITY OF RICE WEEVIL (Sitophilus oryzae) Lisa, Oviana; Lizmah, Sumeinika Fitria; Sari, Putri Mustika; Aminah, Siti; mustaqim, mustaqim
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Storing rice for a long time in the warehouse has the potential to be attacked by warehouse pests, namely rice weevil insect (Sitophilus oryzae). Rice weevil attacked on storage rice caused a decrease in the quality and quantity of rice, so environmentally friendly pest control is needed, such as the used of bioactive plant compounds as botanical insecticides. This research aimed to see the effect of bioactive compounds from the combined powder of fragrant pandan (Pandanus amaryllifolius) and wuluh starfruit (Averrhoa bilimbi) leaves on the mortality of the rice weevil pest S. oryzae. The method of this research used a non-factorial Completely Randomized Design (CRD) experimental method with 4 concentration levels of botanical insecticide combination powder, that were 30, 40, 50 and 60 g. The research results showed that the combination of fragrant pandan and wuluh starfruit leaf powders contained bioactive compounds were steroids, terpenoids, saponins, flavonoids, phenolics and tannins. The activity of the bioactive compounds saponins, flavonoids, phenolics and tannins worked as inhibitors of insect feeding (antifeedant) and caused the mortality of S. oryzae. The highest mortality percentage reaching 85% was obtained after applicated by a concentration of 50 g. The increase in mortality of the S. oryzae pest occurred every week, until mortality reached more than 80% in the 3rd week was obtained in treatment concentrations of 30 g (84.2%) and 50 g (85%).    
ZINGIBERACEAE ETNOBOTANY AS A TRADITIONAL TREATMENT INGREDIENT FOR THE MANDAILING TRIBE IN VILLAGES AROUND THE BATANG GADIS NATIONAL PARK AREA, MANDAILING REGENCY NATAL Sakinah, Indah; Nasution, Aswarina
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.39061

Abstract

ABSTRACKThis study aims to uncover and document belief systems, local knowledge systems, practice of using plants and culturally important values or ICS of plants of the Zingiberaceae family which are used as ingredients for traditional medicine by the Mandailing Tribe in villages around the Batang Gadis National Park area, Mandailing Natal Regency. The research location is in 2 villages around the Batang Gadis National Park, Mandailing Natal Regency. The research method is qualitative and quantitative. Data was collected through semi-structured interviews, participatory observation, documentation, focus group discussions, collection of plant samples and making herbarium. The results showed that there were 10 species of plants from the Zingiberaceae family used as traditional medicine by the Mandailing Tribe. The most widely used species is turmeric or hunik ( Curcuma dometica Val.). Turmeric is believed to cure 8 kinds of diseases. The rhizome organ is the organ that is most widely used with a percentage of 75%. The practice of using plants by the Mandailing Tribe with the most processing method is boiling as many as 10 plant species with a percentage of 35.71% and the most using method by drinking as many as 10 plant species with a percentage of 62.5%. Plants with a very high category of cultural importance or ICS are found in turmeric or hunik ( Curcuma dometica Val.) with an ICS value of 150, ginger or pegeh ( Zingiber officinale Roscoe. ) and red ginger or pegeh narara (Zingiber officinale var. Rubrum) with an ICS value of 120. Keywords: Traditional Medicine, Belief system, Local knowledge, Utilization practice, ICS
DNASe I and structure Catalytic site Nurul Sulviani
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 1 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i1.43429

Abstract

This Article describes 3Dimention studies carried out of catalytic site  over the past year which have advanced of understanding the substrate to recognizition and how the catalytic mechanism of the several key DNAse I. The hydrolysis reaction that occurs in DNASe I is influenced by Mg 2+ and Ca 2+ but let the Binding of DNASe I ions is still unclear, the active side is found, the amount and mechanism of Ca2+ and Mg2+ . The results of the review in this article indicate that DNASe I is needed externally from experimental animals and simulations improve the binding reaction of Ca 2+ and Mg 2+ which is supported by some literature that uses crystallographic studies where Ca 2+ ions can stabilize DNASe I and also in the presence of Mg 2+ ions that can accelerate the hydrolytic failure of the cation. The results of the review explain the role of cations collectively electrostatic matching between DNASe I and DNA.
Effects of grey mangrove leaf extract (Avicennia marina) on the growth of Staphylococcus aureus Priambodo, Bima; Budiarti, Retno; Rahadianto, Rahadianto; Nefertiti, Eva
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.43222

Abstract

The emergence and spread of methicillin-resistant Staphylococcus aureus in recent decades complicates the antibiotic therapy. The grey mangrove (Avicennia marina) has flavonoid, terpenoid, saponin, phenolic, tannin, and alkaloid chemical compounds that have antimicrobial activity, thus potentially inhibiting Staphylococcus aureus. This study aims to examine the effects of the grey mangrove (Avicennia marina) leaf extracts on the growth of Staphylococcus aureus. This research uses laboratory experimental design with disc diffusion method to test the inhibition of Staphylococcus aureus bacteria growth in Mueller-Hinton medium. There were 6 treatment groups, namely negative control of 0.2% DMSO,  positive control of amoxicillin 30 µg, Avicennia marina leaf extract at concentration of 25%, 50%, 75%, and 100%. The results of this research showed that Avicennia marina leaf extract produces inhibition zone of 7.06 mm, 8.51 mm, 10.07 mm, 13.29 mm at concentration of 25%, 50%, 75%, and 100%. Meanwhile, positive control produces inhibiton zone of 23.65 mm, and negative control has no inhibition zone. The statistical tests using one-way ANOVA resulted in a significance value of less than 0.05 (p<α). The conclusion of this study showed that the grey mangrove (Avicennia marina) leaf extract at 100% concentration is the most effective treatment group in inhibiting the growth of Staphylococcus aureus, although the inhibition was not much better when compared to the inhibition of the positive control group.
Phytochemical Screening And Identification of Terpenoids Compounds at Ethanolic Extract Pegagan Leaves (Centella asiatica L. Urban) Astari, Weni
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.57037

Abstract

Terpenoids are one of the secondary metabolite compounds that have animportant role in medicine because they have many benefits in healingvarious diseases. Terpenoids are also believed to be contained in gotu kolaleaves. This research was conducted to find out what are the activecompounds contained in gotu kola leaves. This research begins withextracting 400 grams of gotu kola leaf powder with 96% ethanol solventusing the maceration method. The results of maceration were thenevaporated using a waterbath until a thick extract was obtained.Identification of active compounds contained in gotu kola leaves wascarried out by phytochemical screening test and KLT test on thick extract.In the phytochemical screening test, it is known that the ethanol extract ofgotu kola leaves positively contains terpenoids, steroids and saponins.Then the identification of compounds using the KLT method withethanol:ethyl acetate eluent in a ratio of 9:1, 8:2, 7:3 and 6:4, then sprayedwith reagent liquid and observed under a UV lamp with a wavelength of254 nm. In the ratio of 9:1, the resulting Rf value is 0.64. Then in the ratioof 8:2 and 7:3, the resulting Rf values were 0.67 and 0.69. And in the ratioof 6:4, the resulting Rf value is 0.75. Based on the Rf value obtained, itcan be concluded that the ethanol extract of gotu kola leaves can certainlycontain terpenoid derivative compounds, namely triterpenoids which havea standard Rf value of 0,50-0,89
Analysis of Secondary Metbaolite Content of Ethanol Extract of Biden Pilosa L. Leaves which has the Potential as an Antibacterial Pulungan, Ahmad Shafwan S; Nasution, Aswarina; Puspitasari, Wina Dyah; Gultom, Rahmad H; Rahmi, Nisrina Afiqah; Munthe, Melia Ivana Br
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.62621

Abstract

The potential of plants as an alternative medicine is a great gift. This potential can be explored through various preliminary tests. One of the preliminary tests is to analyze the content of secondary metabolite compounds using GC-MS. One of the plants known to have potential is Biden Pilosa L. The results of the GC-MS analysis showed that there were 9 compounds identified as having antimicrobial properties. This was discovered after analyzing the compound content and continuing with a literature review. The conclusion obtained was that there were 9 compounds in the ethanol extract of Biden Pilosa L leaves that functioned as antimicrobials.
PHYTOCHEMICAL TEST OF SPRUCE ROOT ETHANOL EXTRACT Purba, Eva Dewi Rosmawati
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.60072

Abstract

Spruce root (Casuarinaceae) is known to be useful empirically used as a medicine for hemorrhoids. To determine Phytochemical activity of pine roots, it is necessary to conduct research on “Phytochemical activity of pine roots”.The experimental research with posttest only design. Research site was in the Microbiology and Pharmacognosy Laboratory of the Pharmacy Study Program and the Biology Laboratory of Bangka Belitung University. Research object of Sea spruce roots (Casuarinaceae equisetifolia) were taken in Bangka Botanical Garden in fresh. It then was made into extracts. Research procedures were Plant determination and identification (2) Preparation (3) Standardization of extracts (Casuarinaceae) (4) Phytochemical test. (5) TLC.The results are Phytochemical test showed that the results of the content contained in the spruce root are Alkaloid, Flavonoid, Triterpenoid, Anthraquinone and Tannin.
ANTIMICROBIAL ACTIVITY TEST OF MEDICINAL PLANT EXTRACT USING ANTIMICROBIAL DISC AND FILTER PAPER AGAINST Staphylococcus aureus : CLEAR ZONE RESULTS COMPARISON Hidayat, Muhammad Taufiq; Koentjoro, Maharani Pertiwi; Prasetyo, Endry Nugroho
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.43276

Abstract

Ekstrak tumbuhan mengandung metabolit sekunder berupa flavonoid dan senyawa fenolik yang memiliki aktivitas antibiotik. Uji aktivitas antibiotik dapat dilakukan dengan metode cakram Kirby-Bauer yang aktivitas antibiotiknya ditunjukkan dengan terbentuknya zona bening. Penelitian ini dilakukan dengan menggunakan ekstrak  daun Moringa oleifera  dan  kladode Opuntia cochenillifera  yang diekstrak menggunakan etil asetat sebagai pelarut. Uji aktivitas antibiotik terhadap  bakteri Staphylococcus aureus  dilakukan pada media Mannitol Salt Agar dan Luria Bertani Agar. Cakram Kirby-Bauer yang digunakan adalah Antimicrobial Disc Oxoid® dan Filter Paper. Hasil penelitian ini menunjukkan bahwa penggunaan Antimicrobial disc Oxoid® dan Filter Paper tidak menunjukkan perbedaan secara statistik pada hasil zona bening (t-value = 0,45; p-value = 0,655; dengan α = 95%). Sementara itu, hasil Clear Zone pada Mannitol Salt Agar memberikan hasil yang lebih baik daripada Luria Bertani Agar dan signifikan secara statistik (nilai-t = 2,46; nilai-p = 0,02; dengan α = 95%). Temuan ini menunjukkan bahwa kertas saring dapat menjadi alternatif yang murah untuk uji antibiotik dengan hasil yang tidak berbeda secara signifikan dibandingkan dengan cakram antibiotik komersial. Namun, uji antibiotik terhadap Staphylococcus aureus lebih baik dilakukan pada Mannitol Salt Agar dibandingkan dengan Luria Bertani Agar.
TESTING THE ACTIVITY OF KETUL LEAF (Bidens pilosa L.) ETHANOL EXTRACT IN INHIBITING THE GROWTH OF Escherihia coli BACTERIA Munthe, Melia Ivana; Nasution, Mhd Yusuf; Pulungan, Ahmad Shafwan S
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.52933

Abstract

Daun ketul (Bidens pilosa L.) memiliki senyawa metabolit sekunder yang dimanfaatkan masyarakat dalam mengobati penyakit diare. Daun ketul memiliki senyawa metabolit sekunder antara lain terpenoid, alkaloid, flavonoid, dan fenool yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol daun ketul (B. pilosa L.) dalam menghambat pertumbuhan bakteri Escherichia coli. Penelitian ini menggunakan metode difusi cakram yang terdiri dari 5 variasi konsentrasi yaitu 30%, 40%,50%,60%,70%, dengan 4 kali pengulangan. Data hasil zona hambat  dianalisis dengan uji statistik One Way Anova.  Hasil penelitian menunjukkan ekstrak daun ketul (Bidens pilosa L.) memiliki aktivitas antibakteri  terhadap bakteri Escherichia coli. Semakin luas zona bening yang terbentuk maka semakin kuat senyawa aktif dalam menghambat pertumbuhan bakteri. Berdasarkan hasil uji lanjutan Duncan diperoleh bahwa tidak ada perbedaan secara nyata dari setiap perlakuan konsentrasi dalam menghambat bakteri Escherichia coli.

Page 23 of 27 | Total Record : 269

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