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Ahmad Shafwan S. Pulungan
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Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan Jl. Willem Iskandar Psr V Medan Estate, Sumatera Utara
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JBIO: jurnal biosains (the journal of biosciences)
Published by Universitas Negeri Medan
ISSN : 24431230     EISSN : 24606804     DOI : https://doi.org/10.24114/jbio.v6i1
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Jurnal Biosains (JBIO) features works of exceptional significance, originality, and relevance in all areas of biological science, from molecules to ecosystems, (ie genetic, microbiology, ecology, biosystematic, biostatistic) including works at the interface of other disciplines, such as chemistry, medicine,physic and mathematics. We also welcome data-driven meta-research articles that evaluate and aim to improve the standards of research in the life sciences and beyond. Our audience is the international scientific community as well as educators, policy makers, patient advocacy groups, and interested members of the public around the world.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 269 Documents
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THE EFFECT OF GIVING FERMENTED SAGO DRAIN IN THE RATION ON THE QUALITY OF PEKING DUCK CARCASSES Sari, Wenny Novita; Haryadi, Hayadi; Kamal, Mustafa; Mustaqim, Mustaqim
JURNAL BIOSAINS Vol. 10 No. 3 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i3.63881

Abstract

Ducks are one of the sources of high-quality animal protein, receiving attention in meeting human consumption needs. However, the cultivation of Peking ducks, which have great potential as meat producers, is still carried out extensively and has not been optimal in Indonesia. This study aims to investigate the effect of fermented sago pulp in rations on the quality of carcasses and abdominal fat of Peking ducks. The research method involved the provision of fermented sago pulp in Peking duck rations, with a focus on evaluating the quality of carcasses and abdominal fat. Peking ducks. This study used an experimental method while the design used was a completely randomized design (CRD) with 4 treatments and 3 replications so that there were 12 experimental units. The results showed that the provision of fermented sago pulp in rations showed a significant impact on live weight, carcass weight, carcass percentage, and abdominal fat of Peking ducks. Based on ANOVA analysis and DMRT test, the provision of fermented sago pulp increased all of these parameters at a significance level of P <0.01. Treatment D with 100% fermented sago dregs produced the highest live weight of 1.29 kg and the highest carcass weight of 576.11 gr, significantly different from other treatments.
Identification of Secondary Metabolites of Kirinyuh Leaves (Chromolaena odorata L.) as Antibacterial Using GS-MS grasella, martha; Sulistyarini Gultom, Endang
JURNAL BIOSAINS Vol. 11 No. 1 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i1.64589

Abstract

This study aims to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against pathogenic bacteria and to determine secondary metabolites in kirinyuh leaves (Chromolaena odorata) using the GC-MS (Gas Chromatography-Mass Spectroscopy) method. The test bacteria used were Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa. The extraction method was carried out by maceration using 96% ethanol solvent. The crude ethanol extract of kirinyuh leaves in this study used concentrations of 30%, 40%, 50%, 60% and 70%. Using a UV-VIS spectrophotometer, the most effective concentration in inhibiting and killing the growth of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa bacteria was a concentration of 70%, with an average OD (absorbance) value of -0.2029, -0.055, and -0.0523. Then secondary metabolite identification was carried out using GC-MS (Gas Chromatography-Mass Spectroscopy) ethanol-water (9:1). The test results showed that a concentration of 70% was the most effective in inhibiting and killing Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa bacteria. The content of secondary metabolites in the ethanol extract of kirinyuh leaves contains 33 secondary metabolite compounds, 6 of which are dominant compounds as antibacterials.
Temperature Effects on Jembrana Superficial Unit (JSU) Recombinant Protein Expression in Escherichia coli BL21 Maelani, Imelda; Indriawati, Indriawati; Ismail, Yulia Sari; Mulyani, Riska; Syahfitri, Widya
JURNAL BIOSAINS Vol. 11 No. 1 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i1.64590

Abstract

The efficiency of expression of recombinant proteins in host cells depends on achieving proper protein folding, solubility, and biological activity. Critical environmental factors, including temperature, pH, nutrient availability, and inducer concentration, significantly influence protein yield, stability, and functionality. Optimizing these conditions is essential to minimize protein misfolding and aggregation, which can adversely affect expression efficiency and reduce bioactivity. This study aims to measure the effect of temperature on the growth of Escherichia coli BL21 harboring the recombinant JSU protein, derived from the ENV-SU gene of the Jembrana virus. A controlled laboratory experiment was conducted in which bacterial cultures were incubated at 25°C and 37°C under identical conditions, except for temperature. The results indicate a statistically significant difference in bacterial growth between the two temperature conditions (p = 0.08), suggesting that E. coli BL21 exhibits enhanced growth at 37°C compared to 25°C. The mean bacterial growth rate at 37°C was 1.756, whereas at 25°C, the average growth was 1.425, demonstrating that higher temperatures facilitate bacterial proliferation. However, due to the relatively small sample size, further validation with a larger dataset is required to confirm these findings. These findings highlight the critical role of temperature optimization in enhancing bacterial growth and improving recombinant protein production efficiency, emphasizing the need for further investigation with a larger dataset to validate the results. Keywords: recombinant protein; temperature; IPTG; Escherichia coli BL21; JSU construct.
Antibacterial Activity Of Nutmeg Seed (Myristica fragrans Houtt.) Methanol Extract Against The Growth Of Staphylococcus aureus and Eschericia coli Dewi, Resmila; Kurniaty, Rina; Marniza, Erda
JURNAL BIOSAINS Vol. 11 No. 1 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i1.64594

Abstract

Nutmeg seeds (Myristica fragran Houtt.) are natural materials containing active compounds with potential antibacterial properties. This study aims to determine the antibacterial activity of nutmeg seed methanol extract and the effective concentration for inhibiting the growth of S. aureus and E. coli, as well as to compare the inhibition zones produced against both bacteria. The nutmeg seed extract was obtained through maceration extraction using 96% methanol solvent. The antibacterial activity was tested using the disc diffusion method with three different concentrations of the extract: 20%, 30%, and 40%, along with chloramphenicol (positive control) and 10% DMSO (negative control). The inhibition zone data were analyzed using ANOVA with SPSS 26, followed by the DMRT test. Phytochemical testing revealed that the methanol extract of nutmeg seeds positively contains alkaloids, saponins, flavonoids, quinones, and triterpenoids. The results showed that the methanol extract of nutmeg seeds at concentrations of 20%, 30%, and 40% produced inhibition zones against S. aureus of 11.83 mm, 12.56 mm, and 15.06 mm, respectively, and against E. coli, the inhibition zones measured 11.83 mm, 12.56 mm, and 15.06 mm. The study concludes that the methanol extract of nutmeg seeds exhibits strong antibacterial activity against S. aureus and E. coli, with the most effective concentration being 40%. Additionally, the nutmeg seed methanol extract tended to have better antibacterial properties against E. coli than against S. aureus.
Antibacterial Activity Of Ethanol Extract And Methanol Extract Of Guava Leaves (Psidium guajava L.) Againts Staphylococcus aureus Kurniaty, Rina; Dewi , Resmila; Yusup, Muhilal
JURNAL BIOSAINS Vol. 11 No. 1 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i1.64595

Abstract

Guava leaves contain secondary metabolites, consisting of tannins, polyphenolates, flavonoids, monoterpenoids, alcolloids, quinones, and saponins. The main component of guava leaves is tannins, the amount of which reaches 9-12%. Tannins are antibacterial by precipitating proteins. The antimicrobial effect of tannins is through reactions with cell membranes, inactivation of the function of genetic material. alkaloids, flavonoids can inhibit the growth of Staphylococcus aureus. This research aims to find out the activities antibacterial ethanol and methanol extract of guava leaves against Staphylococcus aureus. This study uses the disc diffusion method. Using 5 treatment groups, namely positive control using chlorampenicol antibiotics and for positive control using DMSO. For concentrations of 5%, 10%, 15% use ethanol extract and methanol guava leaves. This study is an experimental study, testing antibacterial activity using Staphylococcus aureus bacteria. The data obtained was analyzed using the One Way ANOVA test and continued with the DMRT/Ducan test to see the difference in each treatment. The average results of the diameter of the inhibition zone for ethanol extracts were 10.47 mm (5%), 11.95 mm (10%), 16.94 mm (15%). For methanol extracts it is 9.7 mm (5%), 12.1 mm (10%), 19.7 mm (15%). One Way ANOVA analysis followed by DMRT/Ducan showed that both shallot extracts have antibacterial potential and do not have significant differences. The conclusion of this study is that guava leaf ethanol and methanol extracts with concentrations of 5%,10% and 15% have potential as antibacterial against Staphylococcus aureus.
Antibacterial Activity Test of Breadfulness Leaf Extract Plaster Preparation ( Artocarpus altilis ) In Vitro and In Vivo In The Treatment of Mice Cut Wounds Simorangkir, Delisma Marsauli
JURNAL BIOSAINS Vol. 11 No. 1 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i1.64636

Abstract

The skin is the largest organ in the human body. One of the problems that often occurs in the skin is wounds. Wounds are damage or loss of tissue in the body caused by a factor, one type of wound is a cut wound. Cuts occur due to friction with objects causing linear tears in the skin and underlying tissue. Cuts can cause infections due to bacteria. Breadfruit leaves (Artocarpus altilis) contain flavonoid compounds that can regenerate the skin by increasing collagen in the skin and have bactericidal properties. This study aims to determine the optimal concentration in the activity of breadfruit leaf extract formulated in the form of plasters against in vitro and in vivo tests in the treatment of cut wounds in mice. This study used the maceration method in making the extract using 96% ethanol solvent. Breadfruit leaf extract was formulated into a plaster preparation with various concentrations F1: EEDS 10%, F2: EEDS 15%, F3: EEDS 20%, and F4: EEDS 25%. The plaster preparation was evaluated to determine the physical properties and compounds of the wound dressing material by conducting thickness tests, humidity tests, pH tests, organoleptic tests, FTIR tests, activity tests of the preparation against in vitro tests and in vivo tests. The results showed that the concentration of EEDS 25% with a value of 24.35 against the in vitro test using Staphylococcus aureus bacteria with a Hansaplast plaster comparator with a value of 16.35. In the in vitro test in the treatment of mouse cuts, it was stated that the concentration of EEDS 25% with a healing day of 10.6 days was close to the healing day of the Hansaplast plaster comparator with a healing day of 9.3 days.
DISTRIBUTION OF Charybdis (Archias) padangensis Leene & Buitendijk, 1952 (CRUSTACEA: DECAPODA: BRACHYURA) IN THE WATERS OF SUMATRA AND JAVA Hanim, Nisfa; Farajallah, Achmad; Widayat, Kanthi Arum i; Mashar, Ali
JURNAL BIOSAINS Vol. 10 No. 3 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i3.61142

Abstract

Until this decade, Charybdis (Archias) padangensis was only from Padang, West Sumatra. It was reported first and the only one in 1952. We obtained several crab samples based on the by-catch from daily fishermen in several locations. Based on morphological identification, we obtained several Charybdis (Archias) padangensis individuals from two locations in Sumatra and one location in Java. The results of this research add to data on the distribution of the species, which was previously only known from the locality type.
GC-MS ANALYSIS OF SECONDARY METABOLITES OF SENGGANI LEAVES (Melastoma malabatrichum L.) Nasution, Muhammad Yusuf; Rangkuti, Marlinda Nilan Sari; Situmorang, Nurbaity
JURNAL BIOSAINS Vol. 10 No. 3 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i3.62912

Abstract

Traditional medicinal plants have long been used and trusted by the community to treat various diseases. The senggani plant is useful for reducing fever (antispiretic), pain reliever (analgesic), urine laxative (diuretic), treating vaginal discharge (leukorrhea), and as a medicine for various types of wounds. This research was conducted using extracts of Melastoma malabatricum (L.) leaves extracted using methanol as a solvent. GC-MS analysis of secondary metabolites of senggani leaves (Melastoma malabatrichum L.) was carried out as a first step to determine the content of active secondary metabolite compounds contained in senggani leaves.The results of this study show that 8 peaks were observed during maximum run time of 40 min. The results revealed that, compounds such as 3-Hexadecyloxycarbonyl-5-(2-hydroxyethyl)-4-methylimidazolium, Melezitose, Thiosulfuric acid, Neophytadiene, 9-Icosyne, Phytol, Hexadecanoic acid dan 9-Octadecenoic acid (Z) were present in the methanolic extract of Melastoma malabatrichum (L.) which can mostly contribute to several therapeutic activities such as antimicrobial.
PHYSICOCHEMICAL CHARACTERISTICS OF ECO-ENZYME DERIVED FROM DURIAN ALBEDO AS A SOLUTION FOR WASTE MANAGEMENT Harahap, Doli Fadly; Ayu, Gabriella Marry; Putri, Syarifah Riska Mela
JURNAL BIOSAINS Vol. 10 No. 3 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i3.63717

Abstract

North Sumatra is the largest durian-producing province in Indonesia. As a result, durian skin waste has increased, reaching 332,712 tons annually. Durian skin is divided into the outer layer, known as the flavedo and the inner layer called the albedo. Durian skin contains chemical compounds such as lignin and tannins, which hinder its efficient decomposition. Therefore, environmentally friendly solutions for processing durian skin waste are needed. One such solution is the production of eco-enzyme. Eco-enzyme is a complex solution produced by fermenting organic waste with molasses and water, using selective microorganisms. It has various applications, such as household cleaners, organic fertilizers, and pest control. The purpose of this study was to identify the physicochemical characteristics and quality of the eco-enzyme produced, as well as its potential to reduce environmental pollution. The research employed a descriptive qualitative method with an experimental approach. The process involved coarsely chopping durian albedo and mixing it with molasses and water in a 1:3:10 ratio (sugar, albedo, and water). The mixture was fermented for three months, sealed tightly in a container, and periodically opened. The resulting liquid was the eco-enzyme. Physicochemical tests were conducted to evaluate pH, aroma, color, total dissolved solids (TDS) and other properties, including Wagner's Reagent test, Alkaline Reagent test, Foam test, and Quinone test. The results showed that durian albedo is highly suitable for producing eco-enzyme, yielding a product with a pH of 2.4, a TDS value of 855, a blackish-brown color, a fruity fermentation aroma, and containing phytochemical compounds such as alkaloids, flavonoids, saponins, and quinones.
Formulation of Java Acid Leaves (Tamarindus indica L.) Etanol Extract Salep Devices With Absorption, Hydrocarbon, Water Solution and Water Washable Basis (O/W Emulsion) Kurniaty, Rina; Sukma Ratmelya, Dara
JURNAL BIOSAINS Vol. 11 No. 2 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i2.66781

Abstract

Tamarind leaves have flavonoid, tannin and saponin compounds, which can be used as antibacterials. Research has been conducted on the formulation of ointment preparations from tamarind leaf ethanol extract (Tamarindus indica L.) using a variety of ointment bases, namely absorption, hydrocarbon, water soluble and washable with water (O/W emulsions). This study aims to determine the physical stability and the appropriate type of ointment base of tamarind leaf ethanol extract ointment preparation. The extraction process in this study used maceration method with 70% ethanol solvent. The formalized preparation was evaluated for physical stability for 10 days at 4°C and 25°C alternately every 24 hours (1 cycle). The evaluation included organoleptic test, pH, homogeneity and spreadability. Absorption ointment made from ethanol extract of tamarind leaves is light brown in color, smells typical of tamarind, homogeneous, has a pH of 4 and has a spreadability of 5 cm. Hydrocarbon ointment is dark brown in color, smells typical of tamarind, homogeneous, has a pH of 3 and has a spreadability of 3.95 cm. Water soluble ointment with ethanol extract of tamarind leaves is dark brown in color, smells typical of tamarind, homogeneous, has a pH of 3 and has a spreadability of 5.3-5.5 cm. Water washable ointment (O/W emulsion) is light brown in color, smells typical of tamarind, homogeneous, has a pH of 4, and has a spreadability of 3.93 cm. Based on the test results in this study, it can be concluded that the formulation of ethanol leaf extract preparation.

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