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GROWTH PERFORMANCES OF LOBSTER JUVENILE (Cherax quadricarinatus) FED WITH SILKWORM AND RICE FLOUR kurniawan, andri; Adibrata, Sudirman; Lingga, Rahmad; Setiadi, Jhodi; Wulandari, Ufi Ayu; Hidayah, Reysya S N
Journal of Aquatropica Asia Vol 9 No 1 (2024): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/joaa.v9i1.5536

Abstract

Freshwater lobster (Cherax quadricarinatus) is one of the freshwater commodities that has the potential to be cultivated and has economic value. However, the problem still faced by farmers is the quality of feed which can affect low growth and survival. This research aims to analyze the growth performance of juvenile lobsters fed silk worms and rice flour. This research used 30 juvenile lobster samples measuring 1-3 cm (+ 1 inch) that were around 30 days old per aquarium. The parameters observed were body length, body weight, and survival rate (SR) of juvenile lobsters and the water parameters measured were pH, temperature, and total dissolved solids (TDS). The results of the research show that juvenile freshwater lobsters are able to consume silk worms and rice flour so that juvenile lobsters can be classified as omnivores. Feeding treatment in the form of a combination of silk worms and rice flour showed the best results for growth in length, weight and SR of juvenile freshwater lobsters. Juvenile growth during 32 days of rearing fed a combination of silkworm and rice flour resulted 4.30 cm + 0.02 cm in a length, 1.43 g + 0.01 g in weight, and SR 80%.
Identifikasi Bakteri Resisten Cu Dari Sedimen Laut Terdampak Aktivitas Tambang Timah || Identification Of Cu Resistant Bacteria From Tin Mining-Affected Sea Sediment Lingga, Rahmad; Afriyansyah, Budi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 6, No 2: Jurnal Pembelajaran Dan Biologi Nukleus September 2020
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v6i2.1666

Abstract

Aktivitas pertambangan timah di laut memberikan pengaruh buruk terhadap kondisi lingkungan salah satunya adalah cemaran logam berat yang dapat mempengaruhi kehidupan biota perairan. Penelitian ini dilakukan untuk mengisolasi dan menguji resistensi bakteri sedimen laut terdampak aktivitas tambang timah terhadap logam berat Cu. Pengambilan sampel dilakukan pada area penambangan timah di laut sampai ke hamparan lumpur kawasan mangrove terdekat. Isolasi bakteri dilakukan dengan metode cawan sebar dan karakterisasi bakteri meliputi pengamatan bentuk sel, pewarnaan Gram dan uji biokimia. Isolat bakteri selanjutnya diuji resistensinya terhadap logam Cu dengan konsentrasi 10 ppm, 20 ppm, 40 ppm, 80 ppm dan 100 ppm. Hasil penelitian memperlihatkan bahwa isolat bakteri yang berasal dari sedimen laut terdampak aktivitas tambang timah memiliki resistensi terhadap perlakuan logam berat Cu pada berbagai konsentrasi. Isolat B6, B8 dan A10 memperlihatkan resistensi tertinggi terhadap Cu sampai pada konsentrasi 100 ppm.Tin mining activity at sea has various negative influences on the environment. One of them is heavy metal contamination that can affect the life of fisheries biota. This research was conducted to isolate and test the Cu heavy metal resistance of marine sediment bacteria that are affected by tin mining activity. Sediment sampling was carried out in the area of tin mining to the mudflat neared to the mangrove area. Bacterial isolation was carried out by spread plate method and bacterial characterization included cell shape, Gram staining and biochemical tests. Furthermore, bacterial isolates were tested for resistance to metals with concentrations of 10 ppm, 20 ppm, 40 ppm, 80 ppm and 100 ppm. The results showed that bacterial isolates originating from marine sediments affected by mining activity were resistant to Cu heavy metal at various concentrations. Isolates B6, B8 and A10 showed the highest resistance up to the concentration of 100 ppm
Identification of Biofilm-Producing Bacteria From Nangka Island Marine Water in District of Bangka Tengah Robika, Robika; Lingga, Rahmad; Afriyansyah, Budi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 8, No 1: Jurnal Pembelajaran Dan Biologi Nukleus Maret 2022
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v8i1.2492

Abstract

Bacterial community is an important element of an ecosystem both on land and in water. In aquatic ecosystems, bacteria that have the ability to produce biofilms have the advantage of being able to easily attach on a substrate. The main function of biofilm is assisting nutrition absorption from water and facilitating bacteria surviveness from unfavorable environmental conditions. This research was conducted to isolate and identify and test the potential of bacteria to form biofilms in vitro. The methodological steps included measuring the physical-chemical parameters of the waters; isolation by pour plating method; characterization and identification  based on morphological, biochemical and Gram staining; as well as testing the activity of biofilm formation by reading technique using microplate reader. The highest bacterial abundance was found at Station 3 (muddy substrate) which was 3.3x106 cfu/ml. Bacterial isolates that had the highest ability to form biofilms in vitro were Bb3, Bb4 and Bb1 isolates with values of 0.3315, 0.2370) and 0.2131, respectively. Bacteria that have the potential to form biofilms from various substrates in the waters of Nangka Island belong to Alcaligenes and Meniscus genera. From the results of the study, it can be concluded that bacteria from rocky substrates have the ability to produce biofilms better than isolates from other substrates.
Daya Hambat Ekstrak Kasar dan Fraksi Ekstrak Etanol Daun Gribong (Archidendron clypearia (Jack) I.C. Nielsen) Terhadap Bakteri Penyebab Jerawat Insyira, Rania; Lingga, Rahmad; Salmi, Salmi
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12480

Abstract

Acne is a dysbiotic condition caused by Cutibacterium acnes and Staphylococcus epidermidis bacteria. Gribong (Archidendron clypearia (Jack) I.C. Nielson) has been empirically used as a treatment for facial problems by the people of Bangka Belitung. This study aims to test the potential of ethanol extract and fractions of gribong leaves against two bacteria that cause acne. This study is an experimental research conducted in four stages, namely phytochemical testing, antibacterial activity testing, minimum inhibitory concentration testing, and minimum bactericidal concentration testing and equivalence tests against clindamycin antibiotics as positive controls. The results of this study showed (1) Secondary metabolites contained in 96% ethanol extract, ethyl acetate fraction and n-butanol fraction are phenolics, flavonoids, tannins and saponins. Terpenoids were only found in the ethanol extract. The ethyl acetate fraction found no alkaloids and the n-hexane fraction contained only steroids. (2) The 96% ethanol extract, ethyl acetate fraction and n-butanol fraction had antibacterial activity. (3) The highest antibacterial activity was shown by the ethyl acetate fraction with KHM on C. acnes at 0.375% and on S. epidermidis at 1% and KBM for both bacteria at 2.5%. (4) The equivalence value of antibacterial activity of fractions to antibacterial antibiotic clindamycin is still relatively low but still has good antibacterial activity. In conclusion, the ethanol extract and its fractions have potential as anti-acne against C. acnes and S. epidermidis bacteria.
Keanekaragaman Jenis dan Pemanfaatan Jamur Makroskopis di Taman Hutan Raya Bukit Mangkol Desa Teru, Provinsi Kepulauan Bangka Belitung Julian Erika Putri; Lingga, Rahmad; Helmi, Henny
JURNAL BIOS LOGOS Vol. 14 No. 3 (2024): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v14i3.55282

Abstract

Macroscopic and microscopic fungi are living organisms in nature that play an important role in the balance of ecosystems and the surrounding environment. This study aimed to determine the type of macroscopic fungi and the habitat of macroscopic fungi around the Bukit Berambai Hiking trail in the Bukit Mangkol Forest Park Area, as well as to determine the use of macroscopic fungi by the people of Teru Village, Bangka Belitung Province. This research used two methods, namely qualitative and quantitative with field data collection techniques observation surveys, questionnaires, and semi-structured interviews. The research results showed that 66 taxa of macroscopic fungi consisting of two divisions, namely the Ascomycota division and the Basidiomycota division. The dominated fungi was Marasmius sp1. Most of the macroscopic fungi grew and attach to wood substrates, rotting trees, and leaf litter. The people in Teru Village used macroscopic mushrooms as food for consumption, medicine, and tradition. These mushrooms, namely Ear Mushroom (Auricularia sp), Kukur Mushroom (Schizopyllum commune), Tiung Mushroom (Hygrocybe sp 3), White Mushroom (Lentinus sajor-caju), Scale Mushroom (Phylloporus sp.), and Biring Mushroom (Gonoderma sp.).
GREEN SYTHESIS OF CUO USING Citrus x microcarpa BUNGE PEEL EXTRACT AND ANTIBACTERIAL ACTIVITY EVALUATION Afriani, Fitri; Lingga, Rahmad; Rafsanjani, Rifqi Almusawi; Tiandho, Yuant
Science Technology and Management Journal Vol. 5 No. 1 (2025): Januari 2025
Publisher : Fakultas Sains dan Teknologi, Universitas Nasional Karangturi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53416/stmj.v5i1.327

Abstract

This article aims to synthesize CuO nanoparticles using the green synthesis method using Citrus x microcarpa Bunge peel extract, analyze their characteristics, and evaluate their antibacterial activity against the S. aureus. We investigated the role of the accelerator agent NaOH in the green synthesis of CuO nanoparticles. The presence of green synthesized CuO nanoparticles was confirmed by UV-Vis spectrophotometer and XRD. The use of NaOH in the green synthesis of nanoparticles accelerates the emergence of absorbance peaks around 400 nm, related to CuO's surface plasmon frequency. Calculation of crystallite size based on XRD diffractogram shows that NaOH can also act as a reducing agent. The smaller size of CuO nanoparticles will increase their stability when heated. In addition, green synthesized CuO nanoparticles showed high antimicrobial activity against S. aureus pathogens. These results indicate that CuO nanoparticles with antibacterial activity can be synthesized using an environmentally friendly and simple method.
EKSPLORASI POTENSI SUMBERDAYA IKAN LOKAL LELE KELIK (Clarias nieuhofii) SEBAGAI SUMBER PROBIOTIK Adibrata, Sudirman; Lingga, Rahmad; Roanisca, Occa
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 19, No 2 (2023): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.19.2.68-74

Abstract

Pemilihan jenis ikan lokal sebagai sumber probiotik sebaiknya jenis yang sudah beradaptasi terhadap lingkungan alami. Ikan lokal merupakan ikan yang paling tahan terhadap perubahan kondisi cuaca dan keberadaannya masih dapat dijumpai di wilayah tersebut. Penelitian dilakukan bulan Maret hingga September 2022. Lokasi pengambilan sampel di 5 kecamatan di Pulau Bangka yaitu Kecamatan Sungailiat, Merawang, Mendo Barat, Toboali, dan Gabek. Pengujian di Laboratorium Mikrobiologi Universitas Bangka Belitung (UBB) dan CV. Dua Agri Mandiri (CV. DAM) Desa Petaling  Kecamatan Mendo Barat, Kabupaten Bangka. Metode analisis sampel menggunakan rumus kelimpahan relatif, indeks dominansi, kualitas air, dan metode Total Plate Count (TPC) untuk menghitung jumlah mikroba. Hasil menunjukan bahwa terdapat 5 besar sampel ikan yang memiliki kelimpahan relatif tinggi secara berurut yaitu ikan putihan / tawes (Barbonymous gonionotus) 28,05%, ikan lele kelik / lembat (Clarias nieuhofii) 20,73%, ikan nila (Oreochromis niloticus) 9,76%, ikan sepat rawa (Trichogaster trichopterus) 9,76%, dan ikan tepalak (Betta simorum) 7,32%. Nilai indeks dominansi (C) sebesar 0,16 termasuk dominansi rendah artinya tidak ada spesies yang mendominasi secara signifikan dari sampel tersebut. Berdasarkan pertimbangan kelimpahan relatif, indeks dominansi, dan persebaran spesies maka dipilih jenis ikan lele kelik sebagai kandidat isolate BAL probiotik. Kelimpahan ikan air tawar di kolong, rawa, atau sungai ditentukan oleh karakteristik habitat perairannya, begitu juga ikan lele kelik yang hidup di perairan rawa alami dengan entitas kualitas air yang menjadi faktor pembatas. Hal ini diduga bahwa ikan tersebut memiliki toleransi yang luas, kekebalan tubuh yang kuat, serta didukung oleh bakteri baik yang ada dalam tubuhnya. Jumlah mikroba pada sampel lele kelik / lembat (Clarias nieuhofii) yaitu 1,4 x 10⁹ dimana nilai ini lebih tinggi dari jumlah mikroba pada ikan nila, udang vaname, ikan mackerel.The study was conducted from March to September 2022. The locations for sampling were in 5 districts on Bangka Island, namely Sungailiat, Merawang, Mendo Barat, Toboali, and Gabek Districts. The tests were conducted at the Microbiology Laboratory at Universitas Bangka Belitung and CV. DAM in Petaling Village, Mendo Barat District, Bangka Regency. The method used the formula for relative abundance, dominance index, water quality, and the Total Plate Count (TPC) method to calculate the number of microbes. The results showed that there were 5 large samples that had relatively high abundances sequentially, namely tawes (Barbonymous gonionotus) 28.05%, catfish kelik / lembat (Clarias nieuhofii) 20.73%, tilapia (Oreochromis niloticus) 9.76%, swamp fish (Trichogaster trichopterus) 9.76%, and tepalak fish (Betta simorum) 7.32%. The dominance index value is 0.16, including low dominance, meaning that no species dominates significantly from the sample. Based on the relative abundance, dominance index, and species distribution chosen the type of catfish kelik is a candidate for probiotic LAB isolates. The abundance of fish in kolong, swamps, or rivers is determined by the characteristics of their aquatic habitat, like catfish that live in the natural swamp with water quality entities being the limiting factor. It is supposed that these fish had a wide tolerance, and a strong immune system, and were supported by beneficial bacteria in the body of the fish. The number of microbes in the sample of catfish kelik (Clarias nieuhofii) was 1.4 x 10⁹ CFU/ml which was higher than the number of microbes in tilapia, vaname shrimp, mackerel. 
Literature Study of Lactic Acid Bacteria in Bangka Belitung: Sources, Methodology, Utilization, And Identification Lingga, Rahmad; Saputri, Rinny; Fitriyani, Fitriyani; Indra, Muhammad Panca
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i1.5196

Abstract

Bangka Belitung has various types of fermented foods, in the production of which lactic acid bacteria are involved. Lactic acid bacteria plays important role in enhancing the nutritional value of food ingredients, extending their shelf life, producing distinct aromas, and preventing contamination from pathogenic bacteria. Additionally, lactic acid bacteria can also act as probiotics for application in livestock or fisheries. This article examined several studies on lactic acid bacteria that were linked to the biological resources of Bangka Belitung. The aspects studied included the sources of lactic acid bacterial isolates, research methods or approaches employed, utilization, and identification of lactic acid bacteria. Most of the research related to lactic acid bacteria has been conducted on fermented foods, primarily 'rusip,' which is a fermented food product made from shrimp or fish. This review expected to provide an overview of the trends and research interest in lactic acid bacteria conducted in Bangka Belitung, while also mapping out aspects that need further exploration in subsequent research
Edukasi Konservasi Mangrove Bagi Nelayan Sebagai Upaya Menjaga Keberlanjutan Ekosistem Laut Dalimunthe, Nurzaidah Putri; Yurdayanti, Yurdayanti; Martahayu, Vika; Granitio Irwan, Andesta; Juliansyah, Ari; Lingga, Rahmad
Jurnal Pengabdian kepada Masyarakat Nusantara Vol. 6 No. 2 (2025): Jurnal Pengabdian kepada Masyarakat Nusantara Edisi April - Juni
Publisher : Lembaga Dongan Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55338/jpkmn.v6i2.5864

Abstract

Kegiatan pengabdian masyarakat ini dilatarbelakang bahwa Kepulauan Bangka Belitung memiliki kawasan pesisir dengan tutupan mangrove yang berfungsi sebagai habitat burung, udang, dan biota laut pemakan plankton, tempat pembenihan ikan, penyerap polutan, dan menjaga kestabilan pantai. Kabupaten Bangka Selatan, Pulau Lepar, Desa Tanjung Labu memiliki potensi yang besar dalam menghasilkan biota laut sehingga sebagian besar mata pencaharian masyarakat desa sebagai nelayan. Namun, melihat kondisi pesisir pantai yang sedikit keterdapatan habitat mangrove sebagai tempat pemijahan biota, sehingga perlu dilakukan edukasi konservasi mangrove untuk masyarakat pesisir pantai. Pentingnya konservasi mangrove sebagai upaya keberlanjutan ekosistem laut perlu diperhatikan dalam pelestarian kawasan mangrove tersebut. Keberhasilan program konservasi ditentukan oleh adanya partisipasi aktif masyarakat, khususnya para nelayan dan perangkat desa yang terlibat langsung pada mata pencaharian sebagai nelayan. Tujuan dari kegiatan ini, meningkatkan pengetahuan, partisipasi dan inisiatif masyarakat pesisir pantai dan perangkat desa dalam konservasi mangrove diantaranya dengan melakukan edukasi melalui sosialisasi dengan tema konservasi mangrove pada daerah pesisir. Metode yang digunakan adalah metode ceramah dalam edukasi melalui sosialisasi atau penyuluhan. Hasil kegiatan ini bahwa masyarakat mengetahui secara umum tumbuhan mangrove, fungsi mangrove konservasi mangrove, dan keberadaan ekosistem laut terhadap adanya mangrove. Kelompok nelayan dan perangkat desa memperoleh pengaruh positif terhadap kesadaran dan peran aktif masyarakat serta perangkat desa untuk melakukan pelestarian dan penanaman mangrove di sekitar pantai. Namun, perlu adanya pendampingan dalam metode penanaman mangrove yang baik dilihat pada kondisi lingkungan untuk tahapan pengabdian kepada masyarakat selanjutnya.
Eco-friendly feed innovation: An effective strategy to reduce fish mortality rates in Central Bangka Priyansah, Sujadi; Dalimunthe, Nurzaidah Putri; Sembiring, Juhardi; Arif, Maulana; Kurnia, Feni; Syafutra, Randi; Adibrata, Sudirman; Lingga, Rahmad
Community Empowerment Vol 10 No 4 (2025)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/ce.10673

Abstract

Former tin mining land (known locally as camoy or kulong) has the potential to be utilized as fish cultivation land. Kulong possesses potential for fish farming due to its age, which supports the availability of trophic minerals essential for fish life. Fish farmers frequently encounter problems with disease outbreaks and mortality caused by pathogens, leading to low production yields. Improving the quality of the cultivation environment, including reducing sedimentation from commercial feed, is one solution to mitigate fish diseases and mortality. This community service aims to provide training on environmentally friendly feed for fish farmers through training and mentoring in its application. The program successfully enhanced the partners' ability to produce cost-effective (organic) environmentally friendly feed from agricultural waste (oil palm kernel meal, dregs, bran), which demonstrably improved the quality of the cultivation environment, reduced fish diseases and mortality, and has the potential to increase the quality and quantity of fish production.