cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Ahmad Shafwan S. Pulungan
Contact Email
pulungan.shafwan@gmail.com
Phone
+6281370329288
Journal Mail Official
biosains@unimed.ac.id
Editorial Address
Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan Jl. Willem Iskandar Psr V Medan Estate, Sumatera Utara
Location
Kota medan,
Sumatera utara
INDONESIA
JBIO: jurnal biosains (the journal of biosciences)
Published by Universitas Negeri Medan
ISSN : 24431230     EISSN : 24606804     DOI : https://doi.org/10.24114/jbio.v6i1
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Jurnal Biosains (JBIO) features works of exceptional significance, originality, and relevance in all areas of biological science, from molecules to ecosystems, (ie genetic, microbiology, ecology, biosystematic, biostatistic) including works at the interface of other disciplines, such as chemistry, medicine,physic and mathematics. We also welcome data-driven meta-research articles that evaluate and aim to improve the standards of research in the life sciences and beyond. Our audience is the international scientific community as well as educators, policy makers, patient advocacy groups, and interested members of the public around the world.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 269 Documents
 23   24   25   26   27 
ANTIMICROBIAL ACTIVITY TEST OF MEDICINAL PLANT EXTRACT USING ANTIMICROBIAL DISC AND FILTER PAPER AGAINST Staphylococcus aureus : CLEAR ZONE RESULTS COMPARISON Hidayat, Muhammad Taufiq; Koentjoro, Maharani Pertiwi; Prasetyo, Endry Nugroho
JURNAL BIOSAINS Vol. 10 No. 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.43276

Abstract

Ekstrak tumbuhan mengandung metabolit sekunder berupa flavonoid dan senyawa fenolik yang memiliki aktivitas antibiotik. Uji aktivitas antibiotik dapat dilakukan dengan metode cakram Kirby-Bauer yang aktivitas antibiotiknya ditunjukkan dengan terbentuknya zona bening. Penelitian ini dilakukan dengan menggunakan ekstrak  daun Moringa oleifera  dan  kladode Opuntia cochenillifera  yang diekstrak menggunakan etil asetat sebagai pelarut. Uji aktivitas antibiotik terhadap  bakteri Staphylococcus aureus  dilakukan pada media Mannitol Salt Agar dan Luria Bertani Agar. Cakram Kirby-Bauer yang digunakan adalah Antimicrobial Disc Oxoid® dan Filter Paper. Hasil penelitian ini menunjukkan bahwa penggunaan Antimicrobial disc Oxoid® dan Filter Paper tidak menunjukkan perbedaan secara statistik pada hasil zona bening (t-value = 0,45; p-value = 0,655; dengan α = 95%). Sementara itu, hasil Clear Zone pada Mannitol Salt Agar memberikan hasil yang lebih baik daripada Luria Bertani Agar dan signifikan secara statistik (nilai-t = 2,46; nilai-p = 0,02; dengan α = 95%). Temuan ini menunjukkan bahwa kertas saring dapat menjadi alternatif yang murah untuk uji antibiotik dengan hasil yang tidak berbeda secara signifikan dibandingkan dengan cakram antibiotik komersial. Namun, uji antibiotik terhadap Staphylococcus aureus lebih baik dilakukan pada Mannitol Salt Agar dibandingkan dengan Luria Bertani Agar.
DNASe I and structure Catalytic site Sulviani, Nurul
JURNAL BIOSAINS Vol. 10 No. 1 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i1.43429

Abstract

This Article describes 3Dimention studies carried out of catalytic site  over the past year which have advanced of understanding the substrate to recognizition and how the catalytic mechanism of the several key DNAse I. The hydrolysis reaction that occurs in DNASe I is influenced by Mg 2+ and Ca 2+ but let the Binding of DNASe I ions is still unclear, the active side is found, the amount and mechanism of Ca2+ and Mg2+ . The results of the review in this article indicate that DNASe I is needed externally from experimental animals and simulations improve the binding reaction of Ca 2+ and Mg 2+ which is supported by some literature that uses crystallographic studies where Ca 2+ ions can stabilize DNASe I and also in the presence of Mg 2+ ions that can accelerate the hydrolytic failure of the cation. The results of the review explain the role of cations collectively electrostatic matching between DNASe I and DNA.
Effectiveness of Pineapple and Lemon Peel Extracts as Antimicrobial Paper Soap Irawati, Fitri; Nasution, Nabila Halim; Utami, Sari; Pratiwi, Nanda
JURNAL BIOSAINS Vol. 9 No. 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.45380

Abstract

Kesehatan masih menjadi masalah yang memprihatinkan di seluruh dunia. Hal ini juga didukung dengan respon berupa upaya mewujudkan pembangunan skala besar di bidang kesehatan dengan target pencapaiannya pada tahun 2030 yaitu Sustainable Development Goals, menjamin kehidupan yang sehat. Indonesia merupakan negara yang masih mengalami permasalahan lingkungan yang krusial, khususnya sampah. Salah satunya adalah limbah organik kulit buah nanas ( Ananas comosus L.). Penambahan ekstrak lemon ( Citrus limonL.) yang diformulasikan akan memberikan aromaterapi alami dan memberikan efek perasaan tenang. Di sisi lain, pengembangan sabun kertas merupakan inovasi efisiensi dan kemampuan untuk menciptakan kebiasaan mencuci tangan di masyarakat Indonesia. Tujuan dari penelitian ini adalah untuk mengetahui pembuatan sabun kertas dengan bahan alami yaitu limbah lemon dan nanas serta mengetahui bioaktivitas kombinasi tersebut sebagai antiseptik sabun kertas. Metode penelitian ini merupakan metode eksperimen dengan menguji aktivitas antimikroba yang dilakukan dengan metode difusi paper disc technique. Dari penelitian ini dapat disimpulkan bahwa sabun kertas yang terbuat dari bahan alami kulit lemon dan nanas dapat direalisasikan dan memiliki efek antiseptik dengan zona hambat total rata-rata terhadap S. aureus.bakteri sebesar 5,7 mm dan terhadap bakteri E. coli sebesar 20 mm sehingga sabun kertas tergolong efektif dalam menghambat pertumbuhan bakteri patogen
CHARACTERIZATION OF BACTERIA Salmonella sp. ON BROILER CHICKEN MEAT MARKETED AT SUKARAMAI MARKET, MEDAN CITY Rangkuti, Marlinda Nilan Sari; Pulungan, Ahmad Shafwan S; Silaban, Friend
JURNAL BIOSAINS Vol. 9 No. 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.49964

Abstract

One effort to meet high protein needs is obtained from chicken meat. Chicken meat is a food source that is composed of various nutritional values in balanced proportions. There is still doubt about Boiler Chicken meat being sold for sale because it is suspected that there is contamination with pathogenic microbes that can cause disease in humans. There is a risk that broiler chicken meat will be contaminated due to poor market sanitation. The aim of this research was to determine the bacterial contamination of Salmonella sp. in broiler chicken meat and the number of Salmonella sp bacteria. Which is found in samples of broiler chicken meat marketed at the Sukaramai Market, Medan City. This research method is a quantitative descriptive approach. This research was carried out at the Regional Health Laboratory of North Sumatra Province in May-June 2023. In this study, sample culture was carried out by streaking on Salmonella Shigella Agar (SSA) media, while the Total Plate Count (TPC) test for Salmonella sp. using pour plate media. The results showed that all 9 samples contained Salmonella sp. with the amount of bacterial contamination, namely A1 3.0 × 10-4, A2 3.9 × 10-4, A3 4.7 × 10-4, B1 4.0 × 10-4, B2 5.3 × 10-4, B3 6.0×10-4, C1 4.5×10-4, C2 6.8×10-4, C3 7.7×10-4. Based on the National Chicken Meat Standardization Agency ICS No. 67-120-20. In 2009, the maximum limit for microbial contamination and the maximum limit for residues in food ingredients of animal origin must not contain Salmonella sp.
ACTIVITY TEST OF SIAM LABU (Sechium edule (Jacq.) Swartz) LEAF EXTRACT ON REDUCTION OF DARAGE URATE ACID TARGETS OF WHITE RATS (Rattus novergicus) Tambusai, Sofia Rahmi
JURNAL BIOSAINS Vol. 9 No. 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.51002

Abstract

Uric acid is a substance produced from the breakdown of purines or waste products in the body, as a result of purine catabolism aided by the enzymes cguanase and xanthine oxidase. One of the green plants  used as traditional medicine is siam labu (Sechium edule). This plant is a vegetable plant belonging to the Curcubatiacae family that grows in the highlands. The purpose of this study was to test the anti-gout activity of chayote leaves in male  rats. The method used in this study started from preparing simplisia, preparing siam labu leaf extract, inducing mice with Ca-oxanate at a dose of 250 mg/kgBW and administering siam labu leaf extract at a dose of 250 mg/kgBW, 500 mg/kgBW. and 750 mg/kgBW was administered orally with intermittent observation periods of 1 hour for 6 hours. Results obtained in rats given 750 mg/kgBW of extract showed the lowest reduction of 3.88 ± 0.3 mg/dl. One-way ANOVA results  obtained a significant value p=0.00 (p<0.05), which can be concluded that there are differences in each treatment method in experimental animals. Tukey test results can be seen at 6 hours, it can be concluded that EDLS 750 mg/kg body weight with allopurinol is not significantly different.
FIG ENDOPHYTIC BACTERIA PRODUCE ENZYMES AMYLASE, LIPASE, PROOTEASE, CELLULASE Habsari, Lilis; Gultom, Endang Sulistyarini; Nasution, Mhd Yusuf; Rangkuti, Marlinda Nilan Sari; Pulungan, Ahmad Shafwan S
JURNAL BIOSAINS Vol. 9 No. 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.52820

Abstract

The use of enzymes in Indonesia is increasing every year, so action is needed to harmonize the production and needs of enzymes in Indonesia. Enzymes can be produced from various sources such as plants, animals and microorganisms. The advantage of utilizing microorganisms in enzyme production is that they can be produced in large quantities with a shorter time and can be produced continuously. One of the sources of bacterial screening to produce enzymes is figs. This study aims to look at the extracellular enzyme activity of fig endophytic bacteria and identify endophytic bacteria capable of producing extracellular enzymes such as proteases, amylase, cellulase and lipase. Isolation and purification of bacteria using NA media for 24 hours. Test of extracellular enzyme activity using NA media enriched with amylum, tween 80, skim milk, CMC. Biochemical tests are performed to identify endophytic bacteria of figs producing extracellular enzymes. The results of the study were 21 isolates of fig endophytic bacteria, as many as 14 isolates were able to produce extracellular enzymes. The identification results of fig endophytic bacteria producing extracellular enzymes obtained 8 isolates suspected to be from the genus Bacillus, 3 isolates suspected to be from the genus Micrococcus, 1 isolate suspected to be from the genus Cellulomonas, 2 isolates suspected to be from the genus Acetobacter
TESTING THE ACTIVITY OF KETUL LEAF (Bidens pilosa L.) ETHANOL EXTRACT IN INHIBITING THE GROWTH OF Escherihia coli BACTERIA Munthe, Melia Ivana; Nasution, Mhd Yusuf; Pulungan, Ahmad Shafwan S
JURNAL BIOSAINS Vol. 10 No. 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.52933

Abstract

Daun ketul (Bidens pilosa L.) memiliki senyawa metabolit sekunder yang dimanfaatkan masyarakat dalam mengobati penyakit diare. Daun ketul memiliki senyawa metabolit sekunder antara lain terpenoid, alkaloid, flavonoid, dan fenool yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol daun ketul (B. pilosa L.) dalam menghambat pertumbuhan bakteri Escherichia coli. Penelitian ini menggunakan metode difusi cakram yang terdiri dari 5 variasi konsentrasi yaitu 30%, 40%,50%,60%,70%, dengan 4 kali pengulangan. Data hasil zona hambat  dianalisis dengan uji statistik One Way Anova.  Hasil penelitian menunjukkan ekstrak daun ketul (Bidens pilosa L.) memiliki aktivitas antibakteri  terhadap bakteri Escherichia coli. Semakin luas zona bening yang terbentuk maka semakin kuat senyawa aktif dalam menghambat pertumbuhan bakteri. Berdasarkan hasil uji lanjutan Duncan diperoleh bahwa tidak ada perbedaan secara nyata dari setiap perlakuan konsentrasi dalam menghambat bakteri Escherichia coli.
BIOACTIVITY OF FRAGRANT PANDAN AND WULUH STARFRUIT COMBINATION LEAF POWDERS AGAINST THE MORTALITY OF RICE WEEVIL (Sitophilus oryzae) Lisa, Oviana; Lizmah, Sumeinika Fitria; Sari, Putri Mustika; Aminah, Siti; mustaqim, mustaqim
JURNAL BIOSAINS Vol. 9 No. 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.53487

Abstract

Storing rice for a long time in the warehouse has the potential to be attacked by warehouse pests, namely rice weevil insect (Sitophilus oryzae). Rice weevil attacked on storage rice caused a decrease in the quality and quantity of rice, so environmentally friendly pest control is needed, such as the used of bioactive plant compounds as botanical insecticides. This research aimed to see the effect of bioactive compounds from the combined powder of fragrant pandan (Pandanus amaryllifolius) and wuluh starfruit (Averrhoa bilimbi) leaves on the mortality of the rice weevil pest S. oryzae. The method of this research used a non-factorial Completely Randomized Design (CRD) experimental method with 4 concentration levels of botanical insecticide combination powder, that were 30, 40, 50 and 60 g. The research results showed that the combination of fragrant pandan and wuluh starfruit leaf powders contained bioactive compounds were steroids, terpenoids, saponins, flavonoids, phenolics and tannins. The activity of the bioactive compounds saponins, flavonoids, phenolics and tannins worked as inhibitors of insect feeding (antifeedant) and caused the mortality of S. oryzae. The highest mortality percentage reaching 85% was obtained after applicated by a concentration of 50 g. The increase in mortality of the S. oryzae pest occurred every week, until mortality reached more than 80% in the 3rd week was obtained in treatment concentrations of 30 g (84.2%) and 50 g (85%).    
Phytochemical Screening And Identification of Terpenoids Compounds at Ethanolic Extract Pegagan Leaves (Centella asiatica L. Urban) Astari, Weni
JURNAL BIOSAINS Vol. 10 No. 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.57037

Abstract

Terpenoids are one of the secondary metabolite compounds that have animportant role in medicine because they have many benefits in healingvarious diseases. Terpenoids are also believed to be contained in gotu kolaleaves. This research was conducted to find out what are the activecompounds contained in gotu kola leaves. This research begins withextracting 400 grams of gotu kola leaf powder with 96% ethanol solventusing the maceration method. The results of maceration were thenevaporated using a waterbath until a thick extract was obtained.Identification of active compounds contained in gotu kola leaves wascarried out by phytochemical screening test and KLT test on thick extract.In the phytochemical screening test, it is known that the ethanol extract ofgotu kola leaves positively contains terpenoids, steroids and saponins.Then the identification of compounds using the KLT method withethanol:ethyl acetate eluent in a ratio of 9:1, 8:2, 7:3 and 6:4, then sprayedwith reagent liquid and observed under a UV lamp with a wavelength of254 nm. In the ratio of 9:1, the resulting Rf value is 0.64. Then in the ratioof 8:2 and 7:3, the resulting Rf values were 0.67 and 0.69. And in the ratioof 6:4, the resulting Rf value is 0.75. Based on the Rf value obtained, itcan be concluded that the ethanol extract of gotu kola leaves can certainlycontain terpenoid derivative compounds, namely triterpenoids which havea standard Rf value of 0,50-0,89
PHYTOCHEMICAL TEST OF SPRUCE ROOT ETHANOL EXTRACT Purba, Eva Dewi Rosmawati
JURNAL BIOSAINS Vol. 10 No. 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.60072

Abstract

Spruce root (Casuarinaceae) is known to be useful empirically used as a medicine for hemorrhoids. To determine Phytochemical activity of pine roots, it is necessary to conduct research on œPhytochemical activity of pine roots.The experimental research with posttest only design. Research site was in the Microbiology and Pharmacognosy Laboratory of the Pharmacy Study Program and the Biology Laboratory of Bangka Belitung University. Research object of Sea spruce roots (Casuarinaceae equisetifolia) were taken in Bangka Botanical Garden in fresh. It then was made into extracts. Research procedures were Plant determination and identification (2) Preparation (3) Standardization of extracts (Casuarinaceae) (4) Phytochemical test. (5) TLC.The results are Phytochemical test showed that the results of the content contained in the spruce root are Alkaloid, Flavonoid, Triterpenoid, Anthraquinone and Tannin.

Page 26 of 27 | Total Record : 269

 23   24   25   26   27 

Filter by Year

2013 2025


Reset
Filter By Issues
All Issue Vol. 11 No. 3 (2025): JURNAL BIOSAINS Vol. 11 No. 2 (2025): JURNAL BIOSAINS Vol. 11 No. 1 (2025): JURNAL BIOSAINS Vol. 10 No. 3 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES) Vol. 10 No. 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES) Vol 10, No 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES) Vol 10, No 1 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES) Vol. 10 No. 1 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES) Vol 9, No 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences) Vol. 9 No. 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences) Vol 9, No 2 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences) Vol 9, No 1 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences) Vol 8, No 3 (2022): JBIO: jurnal biosains (the journal of biosciences) Vol 8, No 2 (2022): JBIO: jurnal biosains (the journal of biosciences) Vol 8, No 1 (2022): JBIO: jurnal biosains (the journal of biosciences) Vol 7, No 3 (2021): Jurnal Biosains Vol 7, No 2 (2021): Jurnal Biosains Vol 7, No 1 (2021): Jurnal Biosains Vol 6, No 3 (2020): Jurnal Biosains Vol 6, No 2 (2020): Jurnal Biosains Vol 6, No 1 (2020): Jurnal Biosains Vol 5, No 3 (2019): Jurnal Biosains Vol 5, No 2 (2019): Jurnal Biosains Vol 5, No 1 (2019): Jurnal Biosains Vol 4, No 3 (2018): Jurnal Biosains Vol 4, No 2 (2018): Jurnal Biosains Vol 4, No 1 (2018): Jurnal Biosains Vol 3, No 3 (2017): Jurnal Biosains Vol 3, No 2 (2017): Jurnal Biosains Vol 3, No 1 (2017): Jurnal Biosains Vol 2, No 3 (2016): Jurnal Biosains Vol 2, No 2 (2016): Jurnal Biosains Vol 2, No 1 (2016): Jurnal Biosains Vol 1, No 3 (2015): Jurnal Biosains Vol 1, No 2 (2015): Jurnal Biosains Vol 1, No 1 (2015): Jurnal Biosains Vol 1, No 2 (2013): Bio Hasil Review Fauziyah Harahap More Issue