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Beny Irawan
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Medistra Medical Journal (MMJ)
Published by Institut Kesehatan Medistra Lubuk Pakam
ISSN : -     EISSN : 30263492     DOI : https://doi.org/10.35451/mmj.v2i1
Core Subject : Health, Science,
Health Professions Immunology & microbiology Medicine & Pharmacology Public Health
Aims and Scope Medistra Medical Journal (MMJ) Aims to disseminate conceptual thoughts or ideas, reviews and research findings obtained by academics, researchers and practitioners in the field of Medicine and Health Medistra Medical Journal (MMJ) Scope: Biomedical Science & Clinical Practice Preventive Medicine Family Medicine Occupational Medicine Bioethics Trauma and Medical Emergencies Public Health Epidemiology and Biostatistics Mental Health Clinical Nutrition Clinical Pathology Anatomical Pathology
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 56 Documents
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Comparison of the Number of Staphylococcus aureus Colonies in Green Grass Jelly (Cyclea barbata Miers) and Black Grass Jelly (Mesona palustris) Sold in the Lubuk Pakam Area Selda manullang; Yemima Sundita Damanik; Sa’adah Siregar
Medistra Medical Journal (MMJ) Vol 3 No 2 (2026): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/aps49y92

Abstract

Food contamination by pathogenic bacteria is a public health problem that can cause digestive disorders and even serious infections. One of the bacteria commonly found in food is Staphylococcus aureus, which is capable of producing enterotoxins and causing food poisoning. Green grass jelly (Cyclea barbata Miers) and black grass jelly (Mesona palustris) are traditional food products widely consumed by the community and are generally produced and sold in a traditional manner, increasing the risk of bacterial contamination due to poor hygiene practices. This study aimed to compare the number of Staphylococcus aureus colonies in green grass jelly and black grass jelly sold in the Lubuk Pakam area. This research used a laboratory experimental method with a streaking technique on Mannitol Salt Agar (MSA) media using a sterile inoculating loop. Samples were inoculated onto the media and incubated to allow colony growth. Bacterial identification was carried out through macroscopic observation, Gram staining, and biochemical tests including catalase and coagulase tests. The results showed that all samples of green and black grass jelly were positive for Staphylococcus aureus. The total number of colonies in green grass jelly was 465 colonies with an average of 116.25 colonies, while black grass jelly showed a total of 880 colonies with an average of 220 colonies. The difference in the average colony count between the two samples was 103.75 colonies, indicating that black grass jelly had a higher level of contamination than green grass jelly. In conclusion, there is a difference
Analysis of Human Immunodeficiency Virus and Hepatitis B Surface Antigen Examination in Second Trimester Pregnant Women Dian Hastari Ningrum; Asvia Rahayu
Medistra Medical Journal (MMJ) Vol 3 No 2 (2026): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/x64g4h69

Abstract

Infections caused by the Hepatitis B virus and Human Immunodeficiency Virus (HIV) remain major global public health concerns, particularly among pregnant women who are at high risk of transmitting infections to their infants through vertical transmission. Transmission may occur during the intrauterine, intrapartum, or postpartum periods. Screening for Hepatitis B surface antigen (HBsAg) and HIV is an essential component of integrated antenatal care aimed at preventing mother-to-child transmission. The purpose of this study was to analyze the relationship between HIV and HBsAg examination results among second trimester pregnant women at Tanjung Haloban Community Health Center. This study employed an analytical observational design with a cross-sectional approach. A total of 25 respondents were selected using a total sampling technique. Data were obtained from laboratory examination results and analyzed using univariate and bivariate analysis with the Chi-Square test at a significance level of 0.05. The results showed that 88% of respondents had non-reactive HIV results and 80% had non-reactive HBsAg results. Bivariate analysis indicated a statistically significant relationship between HIV and HBsAg examination results (p = 0.02 < 0.05). In conclusion, although the majority of pregnant women were not infected, there were still reactive cases that may contribute to vertical transmission, and a significant association between the two infections suggests the possibility of co-infection. Therefore, strengthening routine screening, improving health education, and optimizing prevention programs are necessary.
Comparison of Urea and Creatinine Levels in Lipemic Serum and Lipemic Serum Treated with Alpha-Cyclodextrin Siti Ramadhani; Vitra Missi Agatha Agatha; Riri Safitri
Medistra Medical Journal (MMJ) Vol 3 No 2 (2026): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/nvvhe105

Abstract

Background: Turbid (lipemic) serum is usually caused by an increase in lipoprotein levels so that turbidity can be clearly seen by the eye. The accumulation of lipoprotein particles causes lipemia in serum, but not all types of lipoproteins. The largest particles, namely chylomicrons with a size of 70-1000 nm, are the main cause of turbidity in serum samples. However, the accumulation of small particles, high density lipoprotein (HDL), low density lipoprotein (LDL) does not produce lipemic serum. Objective: To determine the differences in the results of the examination of urea creatinine levels in lipemic serum and lipemic serum given alpha cyclodextrin, and to determine how to properly handle lipemic serum.  Method: The type of research used in this method is a quantitative experiment with a one group pre and post test design plan. one group pre and post test design is quasi experimental. Results: normality test results show that the data is not normally distributed because the sig value. 0.05, then the research continued with the Friedman's ANOVA test. In the Friedman's ANOVA analysis carried out, the Asyms value was obtained. sig. (0.201) 0.05 Conclusion: So it can be concluded that there is no significant difference between lipemic serum and lipemic serum treated with alpha cyclodextrin in checking urea and creatinine using a chemical analyzer. From the analysis obtained through the Friedman ANOVA test, a sig value of 0.05 was obtained, which means there was no difference in the urea creatinine value in lipemic serum with lipemic serum that was given alpha cyclodextrin. Alpha cyclodextrin can be useful for handling lipemic samples, because alpha cyclodextrin is ale to detect lipids that cause lipemic
ABO Blood Type Examination in Pregnant Women with Anemia at The Nayaka Husada Clinic in 2026 Desy Lustiany Rajagukguk; Sofiani Sari; Siti Hardianti; Dicky Yuswardi
Medistra Medical Journal (MMJ) Vol 3 No 2 (2026): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/15gcwd87

Abstract

Anemia is a clinical condition characterized by a reduced number of erythrocytes, so that the blood's ability to distribute oxygen to body tissues is not optimally fulfilled. According to the World Health Organization (2023), approximately 30% of women aged 15–49 years (around 571 million) and 40% of children aged 6–59 months (269 million) are affected by iron deficiency anemia. The risk of anemia increases with advancing gestational age, particularly among women from lower socioeconomic backgrounds. The prevalence of anemia in Indonesian pregnant women has reached 48.9% in 2020, approaching the threshold for a severe public health problem (≥40%). This study was conducted at Nayaka Husada Clinic, Duri, in March 2026, aiming to determine the distribution of ABO blood group systems among pregnant women with anemia. The study subjects consisted of 20 capillary blood samples analyzed using the Easytouch Hb device. The results indicated that 2 respondents (10%) had normal hemoglobin levels, while 18 respondents (90%) exhibited decreased hemoglobin levels. The distribution of anemia cases based on blood groups revealed that blood group B had the highest proportion, with 8 individuals (40%). This was followed by blood group O with 6 individuals (30%) and blood group A with 5 individuals (25%). The lowest proportion was observed in blood group AB, with only 1 individual (5%). These findings suggest that the prevalence of anemia among pregnant women is higher in individuals with blood group B.
Identification Of Helicobacter Pylori In Gastritis And Escherichia Coli In Gastroenteritis Using The Electrophoresis Method Rahmawati; Juni Masna Santi Giawa; Yudhistira
Medistra Medical Journal (MMJ) Vol 3 No 2 (2026): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/72pcdb07

Abstract

Gastritis and gastroenteritis are common digestive disorders often associated with infections caused by the bacteria Helicobacter pylori (H. pylori) and Escherichia coli (E. coli). Rapid and accurate diagnosis is crucial for determining appropriate management, but conventional methods still have limitations in terms of sensitivity and turnaround time. This study aims to identify the presence of H. pylori DNA in gastritis patients and E. coli DNA in gastroenteritis patients using electrophoresis following DNA amplification via PCR (Polymerase Chain Reaction). This is a laboratory experimental study utilizing 10 patient blood samples, comprising 5 patients diagnosed with gastritis and 5 patients with gastroenteritis. The research procedure included DNA extraction, amplification of target genes (ureA for H. pylori and stx1 for E. coli), and visualization of the results using agarose gel electrophoresis. The results showed that of the 5 samples from gastritis patients, 4 samples (80%) exhibited a 294 bp DNA band, indicating the presence of the H. pylori gene. Meanwhile, all gastroenteritis patient samples (100%) showed a 302-bp DNA band, indicating the presence of the E. coli gene. The electrophoresis results showed clear and consistent DNA bands in identifying both bacteria. The conclusion of this study indicates that the PCR-based electrophoresis method is effective in rapidly and accurately detecting the presence of H. pylori and E. coli in clinical samples, thus having the potential to serve as a reliable molecular diagnostic method to support the diagnosis of gastritis and gastroenteritis.
Mollecular Rapid Test Examination in Suspected Pulmonary Tuberculosis Patients at UPTD Puskesmas Siabu Nisa El Hasanah; Benny Sihombing; Fitri Mulyani Siregar
Medistra Medical Journal (MMJ) Vol 3 No 2 (2026): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/hw6wy170

Abstract

Tuberculosis (TB) is caused by Mycobacterium tuberculosis, causing damage to many body organs, such as the lungs, bones, brain membranes, stomach membranes, intestines, and even the urinary tract. This study aims to achieve early detection of pulmonary tuberculosis cases with rapid and accurate diagnosis at the UPTD Siabu Health Center. To obtain the number of suspected pulmonary tuberculosis patients examined using the Rapid Molecular Test at the UPTD Siabu Health Center Laboratory, from April to May 2025. This type of research is a descriptive analytical study. The results of the study showed that 16.1% (30 out of 187) of patients were confirmed positive for Rifampicin-Sensitive TB through the RMT. The distribution of positive cases was higher in males (16.7%) compared to females (15.2%). The age group ≥40 years had the highest number of TB cases (76.7% of total positives). Respondents with smoking habits showed a higher proportion of TB cases (63.3%) compared to non-smokers. Work as a farmer dominates positive cases (63.3%). In conclusion, these findings support the expansion of TCM implementation in primary healthcare facilities and emphasize the importance of gender-based and socio-environmental approaches in TB control strategies.

Page 6 of 6 | Total Record : 56

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