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Jurnal Analis Kesehatan Kendari
Published by Politeknik Bina Husada Kendari
ISSN : 24607967     EISSN : 27228517     DOI : https://doi.org/10.46356/jakk.v7i1.323
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology
This journal is the Journal of the D3 Medical Laboratory Technology Study Program, Politeknik Bina Husada Kendari which has a scope of knowledge including Clinical Chemistry, Hematology, Microbiology, Parasitology, Cytohistotechnology and Clinical Toxicology. Published twice a year, June and December.
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 85 Documents
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Gambaran Indeks Aterogenik Plasma Pada Penderita Diabetes Melitus Sunaidi, Yanti; Sulfiani; Sefhia Purnama Dita; Muhammad Ihza Lisan Shidqi; Eleventi Oktarina Putri; Antoni Aldo Danendra Sawal
Jurnal Analis Kesehatan Kendari Vol. 8 No. 1 (2025): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 8 (1) Desember 2025)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

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Abstract

Diabetes mellitus is a group of metabolic disorders characterized by elevated blood glucose levels due to abnormalities in insulin secretion or insulin resistance. This condition increases the risk of cardiovascular disease by triggering dyslipidemia, which is marked by elevated cholesterol, increased levels of low-density lipoprotein (LDL), increased triglycerides, and decreased high-density lipoprotein (HDL) in the blood. Dyslipidemia in diabetic patients is associated with a higher Atherogenic Index of Plasma (AIP), which plays a role in the development of atherosclerosis, a key factor in cardiovascular disease. The logarithmic ratio of triglycerides to HDL can be used to determine the AIP, which serves as a marker for assessing atherogenicity and the risk of cardiovascular disease. This study aimed to predict cardiovascular disease in patients with diabetes mellitus through the evaluation of AIP values. The research was descriptive, involving 32 subjects selected using purposive sampling, and conducted at Dr. Tadjuddin Chalid General Hospital, Makassar, South Sulawesi. Based on the results of 32 subjects: 0 subjects (0%) were categorized as having low cardiovascular risk AIP, with an average value of 0; 1 subject (3%) was in the moderate risk category, with an average AIP of 0.19; 31 subjects (97%) were in the high cardiovascular risk category, with an average AIP of 0.82. These findings indicate that most diabetic patients tend to have a high-risk cardiovascular disease, compared to moderate or low-risk categories.
Indeks Eritrosit sebagai Indikator Prognostik Anemia pada Pasien Gagal Ginjal Kronis dengan Hemodialisa di RSUD Bahteramas Provinsi Sulawesi Tenggara Usman, Julianti Isma Sari; Anita Rosanty; Nurul Annisa Febriana Dahlan
Jurnal Analis Kesehatan Kendari Vol. 8 No. 1 (2025): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 8 (1) Desember 2025)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

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Abstract

Chronic kidney disease is a progressive and irreversible decline in kidney function, requiring hemodialysis therapy in the end stage. One of the most common complications is anemia, which can be assessed through erythrocyte indices, including MCV, MCH, and MCHC. This study aims to determine the value of the erythrocyte index as a prognostic indicator in patients with chronic kidney failure with hemodialysis. This research is a descriptive study with a cross-sectional approach. A total of 34 patients were selected using a purposive sampling technique. Blood samples were analyzed using a Sysmex XN-1000 Hematology Analyzer. The results showed normocytic normochromic in 27 patients (79%), microcytic normochromic in 4 patients (12%), and microcytic hypochromic in 3 patients (9%). The average erythrocyte index in patients with chronic renal failure with hemodialysis was found to have an average MCV value of 86.2 fL, MCH 27.5 pg, MCHC 31.8% and hemoglobin 9.1 g/dl which indicates normocytic normochromic anemia. The conclusion of this study is that chronic kidney disease patients undergoing hemodialysis predominantly have normocytic normochromic erythrocyte indices.
Hubungan Antara Hasil Grafik Scattergram Sysmex Xn-1000 Dengan Apusan Darah Tepi Pada Pasien Leukemia Di Rsup Dr. Wahidin Sudirohusodo Makassar Saputra, Agil
Jurnal Analis Kesehatan Kendari Vol. 8 No. 1 (2025): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 8 (1) Desember 2025)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

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Abstract

Leukemia is a hematological malignancy characterized by abnormal proliferation of hematopoietic precursor cells in the bone marrow and an increased number of abnormal white blood cells in peripheral circulation. Early detection is crucial to reduce morbidity and mortality rates, one of which can be achieved through the use of hematology analyzers that display scattergrams. This study aimed to analyze the relationship between scattergram patterns from the Sysmex XN-1000 Hematology Analyzer and peripheral blood smear findings in leukemia patients. A descriptive cross-sectional design with total sampling was employed, involving 13 leukemia patients who underwent complete blood count using the Sysmex XN-1000 and peripheral blood smear examination at Dr. Wahidin Sudirohusodo Hospital, Makassar, from March to May 2019. Data were analyzed descriptively based on the distribution of gender, age, type of leukemia, laboratory results, and scattergram patterns. The results showed that most patients were male (84.6%), the most common age group was 21–40 years (38.5%), and the most frequent leukemia types were Acute Lymphoblastic Leukemia (46.2%) and Chronic Myeloid Leukemia (46.2%). Scattergram analysis revealed distinct patterns for each leukemia type: predominance of high-fluorescence lymphocytes with an inverted comma pattern in ALL, variable-sized myeloid blasts overlapping with granulocytes and monocytes in AML, and granulocytic dominance with immature granulocyte values >10% in CML. These scattergram patterns were consistent with peripheral blood smear findings, supporting their role as an early indicator of hematologic abnormalities. In conclusion, scattergrams from the Sysmex XN-1000 have potential as a rapid and informative screening tool prior to microscopic confirmation in leukemia cases.
Perbedaan Kadar Tumor Necrosis Factor Alpha pada Pekerja Finishing Gitar Berdasarkan Penggunaan Alat Pelindung Diri Dan Lama Bekerja Antoni Aldo Danendra Sawal; Eleventi Oktariani Putri; Muhammad Ihza Lisan Shidqi; Yanti Sunaidi
Jurnal Analis Kesehatan Kendari Vol. 8 No. 1 (2025): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 8 (1) Desember 2025)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

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Abstract

Debu kayu dan zat kimia dari pengecatan gitar selama proses finishing yang masuk ke saluran pernapasan dapat mengaktifkan sistem imun seluler dan saat terjadinya inflamasi menyebabkan pelepasan sitokin pro-inflamasi yaitu Tumor Necrosis Factor Alpha (TNF-α). TNF-α merupakan sitokin pro-inflamasi yang memiliki efek agregasi dan stimulasi neutrofil. TNF-α disekresikan oleh makrofag, yang berperan dalam metabolisme, termasuk diferensiasi, apoptosis, proliferasi sel, koagulasi, dan metabolisme lipid. Penelitian ini merupakan penelitian observasional, dengan rancangan penelitian potong lintang di desa gitar Sukoharjo. Sampel penelitian sebanyak 30 subjek, diambil menggunakan teknik non-probability sampling dengan metode purposive sampling. Pemeriksaan TNF-α dilakukan dengan metode Enzyme-Linked Immunosorbent Assay (ELISA). Hasil penelitian menunjukkan bahwa nilai rata-rata TNF-α pada pekerja finishing gitar yang memakai APD dengan masa kerja 1-3 tahun adalah 66,66 pg/ml, sedangkan pada pekerja yang memakai APD dengan masa kerja >3 tahun adalah 70,71 pg/ml. Nilai rata-rata pada pekerja yang tidak memakai APD dengan masa kerja 1-3 tahun adalah 71,64 pg/ml, sedangkan pada pekerja yang tidak memakai APD selama >3 tahun adalah 86,10 pg/ml. Kesimpulan dari penelitian ini adalah terdapat perbedaan kadar TNF-α berdasarkan penggunaan APD yang bekerja selama 1-3 tahun maupun >3 tahun.
Analisis Retrospektif Hasil Pemeriksaan Tiroid (TSH dan FT4) dengan Metode ELFA di RSUD Dr. Doris Sylvanus Palangka Raya Eleventi Oktarina Putri; Antoni Aldo Danendra Sawal; Muhammad Ihza Lisan Shidqi; Yanti Sunaidi
Jurnal Analis Kesehatan Kendari Vol. 8 No. 1 (2025): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 8 (1) Desember 2025)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

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Abstract

Thyroid disorders are among the most prevalent endocrine conditions globally, demanding precise diagnostic methodologies for effective management. Epidemiological data on thyroid disorders in Kalimantan is still limited. This study aims to re-analyze retrospective data on .thyroid stimulating hormone (TSH) and free thyroxine (FT4) levels measured using the Enzyme Linked Fluorescent Assay (ELFA) method in 2017 at RSUD Dr. Doris Sylvanus Palangka Raya, evaluate them with the latest guidelines, and validate the ELFA method. Data from 87 patients with suspected thyroid dysfunction were subjected to descriptive statistics, Chi-square tests, Spearman's correlation, and Receiver Operating Characteristic (ROC) curve analysis. Patients were predominantly female (n=67, 77%). Hormone distribution showed a tendency towards hyperthyroidism, with 48.3% (n=42) of patients having low TSH and 27.6% (n=24) having high FT4. Statistical analysis showed no significant association between gender and TSH (χ² = 1.277, df = 2, p = 0.528) or FT4 (χ² = 3.240, df = 2, p = 0.198) levels. However, a very strong and significant physiological relationship was found between TSH and FT4 levels, both categorically (χ² = 24.576, df = 4, p < 0.001) and correlationally (r = -0.503, p < 0.001). ROC analysis proved that the ELFA method has very good diagnostic accuracy with an Area Under Curve (AUC) of 0.915 (95% CI: 0.835–0.995, p < 0.001). The profile of thyroid disorders in this region is dominated by a hyperthyroidism pattern, and historical data from the ELFA method has been proven to remain valid and clinically relevant for epidemiological studies. These findings affirm that data generated by ELFA technology in 2017 remains robust and clinically valid when interpreted through the lens of modern guidelines. The study underscores the value of simultaneous TSH-FT4 testing and supports the sustained utility of older, yet reliable, immunoassay technologies, particularly in resource-limited settings, contributing to longitudinal epidemiological understanding of thyroid disorders in the region.
Keandalan Metode Kamar Hitung, Fonio, dan Hematology Analyzer dalam Pemeriksaan Jumlah Trombosit pada Sampel Eritropenia Purbasari, Euis; Erikardo, Oktadio; Nikmatullah, Nurul Azmah; Ardiansyah
Jurnal Analis Kesehatan Kendari Vol. 8 No. 1 (2025): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 8 (1) Desember 2025)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

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Abstract

Pemeriksaan jumlah trombosit secara manual masih menjadi metode yang dapat diandalkan, terutama untuk mengkonfirmasi hasil pemeriksaan automatic yang dicurigai kurang akurat. Namun, hasil pemeriksaan manual dapat dipengaruhi oleh berbagai faktor, termasuk kondisi penyakit pasien. Salah satu kondisi yang mungkin berpengaruh adalah eritropenia, terutama terkait metode perhitungan eritrosit tidak langsung. Meski demikian, pengaruh eritropenia terhadap keandalan pemeriksaan trombosit secara manual dan dampaknya belum banyak diteliti. Penelitian ini bertujuan untuk menganalisis perbedaan hasil hitung trombosit menggunakan metode manual langsung kamar hitung, metode manual Fonio, dan metode automatic (hematology analyzer) pada sampel eritropenia. Penelitian dilakukan pada 11 sampel darah eritropenia yang dipilih secara purposive sampling dan jumlah trombosit pada seluruh sampel dilakukan menggunakan 3 metode yang berbeda. Hasil uji One-Way ANOVA menunjukkan terdapat perbedaan yang signifikan p=<0,001, dengan rerata tertinggi pada metode automatic (302.000 sel/µL), kemudian diikuti metode langsung (254.000 sel/µL), dan jumlah terendah pada metode tidak langsung (134.032 sel/µL). Uji Post Hoc LSD menunjukkan perbedaan yang signifikan hanya terjadi antara metode manual Fonio dengan metode manual kamar hitung dan automatic. Penelitian ini menyimpulkan bahwa metode automatic dan manual secara langsung memberikan hasil yang lebih dapat diandalkan dibandingkan metode tidak langsung, karena metode tidak langsung bergantung pada jumlah eritrosit.
Uji Efektivitas Ekstrak Daun Twandokulo (Kleinhovia hospita Linn) Terhadap pertumbuhan Bakteri Streptococcus mutans ATCC 25175 Penyebab Karies Gigi Nur Awalia Putri Zainal; Ainun ayu yuniar; Angriani Fusvita; Rein Rahmat
Jurnal Analis Kesehatan Kendari Vol. 8 No. 1 (2025): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 8 (1) Desember 2025)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v8i1.395

Abstract

leaf is one of the local medicinal plants of Southeast Sulawesi, which has several benefits. One of the benefits of the Kleinhovia hospita Linn plant is as an antibacterial. The purpose of this study was to determine the antibacterial effectiveness of tawandokulo leaf extract (Kleinhovia hospita Linn) on the inhibition zone of Streptcoccus mutans ATCC 25175 bacteria. This research is a laboratory experiment to see the antibacterial effectiveness test of tawandokulo leaf extract (Kleinhovia hospita Linn) against Streptcoccus mutans ATCC 25175 bacteria at various concentrations namely 20%, 40%, 60%, positive control Amoxcilin 0.001%, negative control DMSO. Tawandokulo leaf extract was obtained from maceration extraction using ethanol solvent. This study used the agar well diffusion method, incubated at 37oC for 24 hours and the inhibition was measured using a caliper, with extract samples carried out in triplicate. Based on the antibacterial effectiveness test of Tawandokulo Leaf Extract (Kleinhovia hospita Linn) against Streptcoccus mutans ATCC 25175 bacteria, the inhibition response results were obtained at concentrations of 20%, 40%, and 60%. The results obtained showed that the average diameter of the inhibition zone from the growth of Streptcoccus mutans ATCC 25175 bacteria obtained at 20% concentration had no inhibition zone, 40% concentration had no inhibition zone, 60% concentration had an average inhibition zone of 6,23 mm, positive control had an average inhibition zone of 19,13 mm, and negative control had no inhibition zone.
Profil Kadar Glukosa Darah pada Lansia di Kecamatan Rambipuji Kabupaten Jember Desi Wulandari; fina Lanahdial Husna; Aulia Fitri
Jurnal Analis Kesehatan Kendari Vol. 8 No. 2 (2026): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 8 (2) June 2026)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v8i2.421

Abstract

Elderly individuals are a population group vulnerable to increased blood glucose levels due to the aging process, decreased insulin sensitivity, and changes in body metabolism. Random blood glucose (RBG) examination is important as an early detection effort for hyperglycemia and diabetes mellitus in the elderly. The objective of this academic inquiry was to delineate random blood glucose levels within geriatric populations according to age and gender. The investigation executed an observational technique utilizing a cross-sectional methodology. The selected cohort comprised 25 advanced-age participants whose random blood glucose levels were quantified a single time utilizing capillary blood samples. Collected information underwent statistical summarization via frequency distribution and percentages. The empirical outcomes demonstrated that subjects within the 60–74 years bracket accounted for 17 individuals (68%), whereas those within the 75–90 years classification constituted 8 persons (32%). Categorized by gender, 19 participants (76%) were female and 6 subjects (24%) were male. Normal blood glucose levels were identified in 13 examinees (52%), whereas elevated blood glucose levels were documented in 12 candidates (48%). The 75–90 years generational cluster exhibited an increased ratio of elevated blood glucose levels, mounting to 62.5%. In relation to gender, males demonstrated a superior fraction of elevated blood glucose levels (66.7%) parallel to females (42.1%). Practically half of the senior enrollees manifested elevated blood glucose levels. Older elderly individuals and males had a higher proportion of hyperglycemia compared to other groups. Routine blood glucose examination and the implementation of a healthy lifestyle are necessary to prevent complications of diabetes mellitus in the elderly.
Gambaran Hasil Pemeriksaan HCG Metode Imunokromatografi dan Aglutinasi Lateks pada Sampel Urin Pagi pada Ibu Hamil Trimester Pertama Yonahara Farahita; Tantri Ramadan; Yoen Ubay Maulidias Sudarjo; Nafi Izza Putri; Arimbi Eka Saputri; Ica Fikri Musyarofah; Zalfa Aliyah Salsabilah; Fu'ad Minan Zuhri
Jurnal Analis Kesehatan Kendari Vol. 8 No. 2 (2026): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 8 (2) June 2026)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v8i2.425

Abstract

Pregnancy is a physiological process characterized by an increase in Human Chorionic Gonadotropin (HCG) hormone concentrations in blood and urine, making HCG examination essential for early pregnancy detection. This study aimed to describe the results of HCG examination using immunochromatography and latex agglutination methods on morning urine samples from first-trimester women in pregnancy. This research used a cross-sectional, descriptive observational design carried out at Banyumas Regency’s Medical Laboratory Technology Laboratory. The study population consisted of first-trimester pregnant women, with a total sample of 24 respondents selected using purposive sampling. Morning urine samples were examined using immunochromatography and latex agglutination methods according to standard operating procedures, and the results were provided as frequency and percentage distributions after a descriptive analysis. The results showed that all samples tested positive using the immunochromatography method, indicating the presence of HCG in all respondents. The latex agglutination method demonstrated varying HCG titers, with the highest frequency observed at a dilution of 1:32, reflecting differences in HCG concentration among samples. These variations were influenced by gestational age and increasing hormone levels during the first trimester. It can be concluded that both immunochromatography and latex agglutination methods are effective in detecting HCG in morning urine samples of first-trimester pregnant women. It is recommended to use morning urine and appropriate testing methods to obtain optimal early pregnancy detection results.
Pengaruh Lama Fiksasi Neutral Buffered Formalin 10% Terhadap Kualitas Hasil Pewarnaan Hematoksilin Eosin Jaringan Mioma Uteri di RSUD Wangaya Ketut Devita; Anak Agung Ayu Eka Cahyani; Putu Gede Subhaktiyasa
Jurnal Analis Kesehatan Kendari Vol. 8 No. 2 (2026): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 8 (2) June 2026)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v8i2.427

Abstract

Uterine fibroids are benign tumors originating from the smooth muscle of the uterus, and their diagnosis can be established through histopathological examination using Hematoxylin and Eosin (H&E) staining. One of the important stages in tissue preparation is the fixation process using 10% Neutral Buffered Formalin (NBF), as the duration of fixation may affect the quality of tissue staining. This study aimed to determine the effect of 10% NBF fixation duration on the quality of Hematoxylin and Eosin (H&E) staining in uterine fibroid tissue with fixation times of 12, 24, and 48 hours.This study employed an analytical experimental design and was conducted at Wangaya Regional General Hospital from January to February. Data were analyzed using the Shapiro–Wilk normality test, which showed that the data were not normally distributed; therefore, the Kruskal–Wallis test was subsequently performed. The results showed a p-value of 0.006, indicating a significant difference among the groups. Post hoc analysis using the Mann–Whitney test demonstrated significant differences between the 12-hour and 24-hour fixation groups (p = 0.047) and between the 12-hour and 48-hour fixation groups (p = 0.007), whereas no significant difference was found between the 24-hour and 48-hour fixation groups. The results indicate that the duration of 10% NBF fixation affects the quality of Hematoxylin and Eosin (H&E) staining in uterine fibroid tissue, in which fixation for 24–48 hours provides better staining quality than fixation for 12 hours. This study is expected to serve as a reference for determining tissue fixation duration in routine histopathological examinations and for further research regarding tissue fixation processes and the quality of Hematoxylin and Eosin (H&E) staining. Keywords: Fixation Time, 10% Neutral Buffered Formalin, H&E Staining.

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