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FIRDAYANTI
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+6285241533218
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Kota kendari,
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Jurnal Analis Kesehatan Kendari
ISSN : 24607967     EISSN : 27228517     DOI : https://doi.org/10.46356/jakk.v7i1.323
Core Subject : Health, Science,
This journal is the Journal of the D3 Medical Laboratory Technology Study Program, Politeknik Bina Husada Kendari which has a scope of knowledge including Clinical Chemistry, Hematology, Microbiology, Parasitology, Cytohistotechnology and Clinical Toxicology. Published twice a year, June and December.
Articles 69 Documents
ANALISIS ANALISIS KADAR INTERLEUKIN 6 (IL-6) PADA PASIEN HEPATITIS B DI KLINIK UTAMA MATA JEC ORBITA MAKASSAR Kasmuddin Darmo; Arlitha dekayana; Judmainnah judmainnah; Risky Nurul Fadlila RN; Amira Aznawi
Jurnal Analis Kesehatan Kendari Vol. 5 No. 2 (2023): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 5 (2) Juni 2023)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v5i2.230

Abstract

ABSTRACT Hepatitis B is an inflammation of the liver caused by the hepatitis B virus. When inflammation occurs, cytokines in the body will respond or recognize the type of pathogen in the form of a virus that enters the body and Interleukin 6 (IL 6) is a cytokine secreted from tissues. body during acute or chronic infection. Interleukin 6 (IL- 6) is a proinflammatory cytokine. The purpose of this study was to analyze the levels of Interleukin 6 (IL-6) in hepatitis B patients using a descriptive analytic method. The samples used were serum samples in Hepatitis B patients as many as 20 patients who were adjusted to the inclusion and exclusion criteria using the ELISA (Enzyme Linked Immunosorbent Assay) method. The results of this study obtained that the highest interleukin 6 level was 153.67 pg/mL and the lowest interleukin 6 level was 1.37 pg/mL. Hepatitis B patients with normal interleukin 6 levels were 12 patients with a percentage of 60%, while those with high interleukin 6 levels were 8 patients with a percentage of 40%. Interleukin 6 does not cause hepatitis B but interleukin 6 and hepatitis B are related. Increased levels of interleukin 6 can be used as an indicator to predict the risk of acute hepatitis B. Keywords: Hepatitis B, Indicator, Interleukin 6 (IL-6), ELISA ABSTRAK Hepatitis B adalah peradangan pada organ hati yang disebabkan oleh virus hepatitis B. Pada saat terjadi inflamasi, sitokin yang ada dalam tubuh akan merespon atau mengenali jenis patogen berupa virus yang masuk ke dalam tubuh dan Interleukin 6 (IL 6) adalah sitokin yang disekresikan dari jaringan tubuh ketika terjadi infeksi akut atau kronik. Interleukin 6 (IL-6) merupakan sitokin proinflamasi. Tujuan dari penetilian ini adalah untuk menganalisis kadar Interleukin 6 (IL-6) pada pasien hepatitis B dengan metode deskriptif analitik. Sampel yang digunakan yaitu sampel serum pada pasien Hepatitis B sebanyak 20 pasien yang disesuaikan dengan kriteria inklusi dan ekslusi dengan menggunakan metode ELISA (Enzyme Linked Immunosorbent Assay). Hasil dari penelitian ini diperoleh kadar interleukin 6 tertinggi yaitu sebanyak 153.67 pg/mL dan kadar interleukin 6 terendah yaitu sebanyak 1.37 pg/mL. Pasien Hepatitis B dengan kadar interleukin 6 normal sebanyak 12 pasien dengan persentasi sebanyak 60%, sedangkan dengan kadar interleukin 6 tinggi sebanyak 8 pasien dengan persentasi 40%. Interleukin 6 bukan penyebab hepatitis B tetapi interleukin 6 dan hepatitis B merupakan sesuatu yang berhubungan. Meningkatnya kadar interleukin 6 bisa dijadikan indikator untuk memprediksi resiko hepatitis B akut. Kata Kunci : Hepatitis B, Indikator, Interleukin 6 (IL-6), ELISA
HUBUNGAN KETON URINE DENGAN GLUKOSA DARAH PADA PASIEN DIABETES MELLITUS DI LABORATORIUM KLINIK PRODIA DENPASAR Niputu Pina Martini
Jurnal Analis Kesehatan Kendari Vol. 5 No. 2 (2023): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 5 (2) Juni 2023)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v5i2.249

Abstract

ABSTRACT Diabetes is a chronic metabolic disease characterized by increased blood glucose levels (hyperglycemia) caused by the pancreas not producing enough insulin for the body or the body experiencing insulin resistance. Too high glucose levels and a lack of the insulin hormone in diabetics can cause the body to use fat as an energy source. In the breakdown of fat, ketone are produced as a secondary product. If ketone produced in excessive amounts, it can be detected in the blood and urine (ketonuria). This study aimed to determine the relationship between urine ketones and blood glucose in patients with diabetes mellitus at the Prodia Denpasar clinical laboratory. The type of research used in this study was a non- experimental correlational with cross sectional design. The sample used is secondary data of diabetic patients for a total of 33 with purposive sampling technique. The results showed a p value of 0.575 which means that there is a relationship between urine ketones and blood glucose in diabetes mellitus patients with a strong, unidirectional and significant correlation. Keywords: Diabetes Mellitus, Urine Ketone, Blood Glucose ABSTRAK Diabetes mellitus (DM) merupakan gangguan metabolisme kronis yang ditandai dengan tingginya kadar glukosa darah (hiperglikemia) karena pankreas tidak mampu memproduksi insulin atau tubuh resisten terhadap insulin Kadar glukosa yang terlalu tinggi dan kekurangan hormon insulin pada penderita DM menyebabkan tubuh menggunakan lemak sebagai sumber energi. Proses pemecahan lemak, akan menghasilkan badan keton yang jika berlebihan dapat dideteksi dalam darah dan urin (ketonuria). Penelitian ini bertujuan untuk mengetahui hubungan keton urine dengan glukosa darah pada pasien diabetes mellitus di laboratorium klinik Prodia Denpasar. Jenis penelitian yang digunakan dalam penelitian ini yaitu non eksperimental korelasional dengan rancangan cross sectional. Sampel yang digunakan merupakan data sekunder pasien dengan diabetes mellitus sejumlah 33 pasien dengan teknik sampling purposive sampling. Hasil penelitian menunjukkan p value sebesar 0,575 yang berarti terdapat hubungan keton urine dengan glukosa darah pada pasien diabetes mellitus dengan korelasi yang kuat, bersifat searah dan signifikan. Kata Kunci : Diabetes Mellitus, Keton Urine, Glukosa Darah
GAMBARAN KADAR SERUM GLUTAMIC OXALOACETIC TRANSAMINASE (SGOT) PADA PENDERITA HIV YANG MENGGUNAKAN OBAT ANTIRETROVIRALDI KOTA KENDARI Susanti Susanti; Muh Ilyas Yusuf; wahyu ningsih
Jurnal Analis Kesehatan Kendari Vol. 5 No. 2 (2023): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 5 (2) Juni 2023)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v5i2.252

Abstract

ABSTRACT HIV patients’ need treatment with Antiretroviral (ARV) to reduce the number of HIV viruses in the body so as not to enter the AIDS stage, whereas people with AIDS need ARV treatment to prevent opportunistic infections with their complications. ARV drugs can causes an increase in liver enzymes in HIV patients’ who cause liver damage related to antiretoviral drug-related liver injury (ARLI). HIV starts with an acute infection that cannot be overcome by an adaptive immune response and causes complications or disorders of various organs of the body, one of them is liver. One important complication of HIV infection after pneumia and sepsis. Damage to liver cells will be followed by the removal of enzymes including SGOT and SGPT. This study aims to determine the description of SGOT levels to HIV patients’ who used antiretroviral drugs. The type of this study used descriptive and the technique of sampling used Accidental Sampling with 20 samples. Based on the results of this study of 20 patients, showed that HIV patients’ who has abnormal SGOT levels as much as 7 people(35%) male and 2 people(10%) female. Keywords: Serum Glutamic OxaloaceticTransaminase (SGOT), Human Immunodeficiency Virus (HIV) ABSTRAK Penderita HIV memerlukan pengobatan dengan Antiretroviral (ARV) untuk menurunkan jumlah virus HIV didalam tubuh agar tidak masuk kedalam stadium AIDS, sedangkan pengidap AIDS memerlukan pengobatan ARV untuk mencegah terjadinya infeksi oportunistik dengan berbagai komplikasinya. Obat ARV dapat menyebabkan peningkatan enzim hati pada penderita HIV yang menyebabkan kerusakan hati terkait obat antiretroviral (antiretoviral drug-related liver injury/ ARLI).Penyakit HIV dimulai dengan infeksi akut yang tidak dapat diatasi oleh respon imun adiptif dan menyebabkan komplikas I atau gangguan pada berbagai organ tubuh salah satunya organ hati.Salah satu komplikasi penting dari infeksi HIV setelah pneumia dan sepsis. Kerusakan sel-sel hati akan diikuti oleh pengeluaran enzim-enzim antara lain SGOT dan SGPT. Penelitian ini bertujuan untuk mengetahui gambaran kadar SGOT pada penderita HIV yang menggunakan obat antiretroviral. Jenis penelitian ini adalah penelitian deskriptif dengan teknik pengambilan sampel secara Accidental Sampling dengan jumlah sampel sebanyak 20 sampel. Berdasarkan hasil penelitian terhadap 20 sampel menunjukan bahwa penderita HIV yang memiliki kadar SGOT tidak normal sebanyak 9 orang (45% ), 7 orang (35%) pada jenis kelamin laki-laki dan 2 orang (10%) pada perempuan. Kata Kunci: Serum Glutamic OxaloaceticTransaminase (SGOT), Human Immunodeficiency Virus (HIV)
SKRINING FITOKIMIA DAN UJI DAYA HAMBAT EKSTRAK PAKKAT UMBUT ROTAN (Callamus Caesius Blume) TERHADAP PERTUMBUHAN Staphylococcus aureus Ani Umar; Angriani Fusvita; Sri Rahayu
Jurnal Analis Kesehatan Kendari Vol. 5 No. 2 (2023): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 5 (2) Juni 2023)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v5i2.253

Abstract

ABSTRACT Rattan tubers (Calamus caesius Blume) is a traditional food taken from the inside of young rattan which is usually used as vegetables. The growth of Staphylococcus aureus bacteria can be inhibited by the content of various chemical compounds in various plants such as rattan shoots. The rattan shoots contain saponins and alkaloids. This study aims to determine the phytochemical screening and test the inhibition of rattan root extract (Callamus Caesius Blume) on the growth of Staphylococcus aureus. The type of research carried out was an experiment with rattan umbut extract treated as a test of inhibition of the growth of Staphylococcus aureus ATCC 25923. 500 grams of rattan umbut pakkat extract was obtained by maceration method for 5 days. In this study using the Kirby Bauer method (disk diffusion). The results showed that the chemical compounds contained in the rattan umbut pack were alkaloids and saponins, while in the inhibition test there was no clear zone around the paper disk which indicated that the rattan umbut pack extract could not inhibit the growth of Staphylococcus aureus with a concentration of 5%, 10%, 15% , and 100% . Key words: Inhibition zone, rattan root extract, Staphylococcus aureus ABSTRAK Pakkat umbut rotan (Calamus caesius Blume) adalah makanan tradisional yang diambil dari bagian dalam rotan muda yang biasanya dijadikan sayur-sayuran. Pertumbuhan bakteri Staphylococcus aureus dapat dihambat oleh kandungan senyawa kimia yang beragam pada berbagai tumbuhan seperti umbut rotan. Umbut rotan tersebut memiliki senyawa saponin dan alkaloid. Penelitian ini bertujuan untuk mengetahui skrining fitokimia dan uji daya hambat ekstrak pakkat umbut rotan (callamus Caesius Blume) terhadap pertumbuhan Staphylococcus aureus. Jenis penelitian yang dilakukan adalah eksperimen dengan ekstrak pakkat umbut rotan diberikan perlakuan sebagai uji daya hambat terhadap pertumbuhan Staphylococcus aureus ATCC 25923. Ekstrak pakkat umbut rotan sebanyak 500 gram diperoleh dengan metode maserasi selama 5 hari. Pada penelitian ini menggunakan metode Kirby bauer (difusi disk). Hasil penelitian menunjukan bahwa senyawa kimia yang terdapat pada pakkat umbut rotan adalah alkaloid dan saponin sedangkan pada uji daya hambat tidak terdapat zona bening disekitar paper disk yang menandakan ekstrak pakkat umbut rotan tidak dapat menghambat pertumbuhan Staphylococcus aureus dengan konsentrasi 5%, 10%, 15%, dan 100% . Kata kunci: Zona Hambat, ekstrak pakkat umbut rotan, Staphylococcus aureus
HUBUNGAN INDEKS MASSA TUBUH DENGAN KADAR ALBUMIN PADA PENDERITA TUBERKULOSIS PARU YANG MENJALANI TERAPI OBAT ANTI TUBERKULOSIS (OAT) DI RUMAHSAKIT BENYAMIN GULUH KOLAKA Kemal Idris Balaka; Apriyanto Apriyanto; Indriani Ishar
Jurnal Analis Kesehatan Kendari Vol. 5 No. 2 (2023): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 5 (2) Juni 2023)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v5i2.261

Abstract

Albumin is one indicator of malnutrition (malnutrition), both at the beginning of the incidence of malnutrition and when repairs begin to occur. Tuberculosis is a contagious infectious disease caused by the bacterium Mycobacterium tuberculosis, which can attack various organs, especially the lungs. Tuberculosis itself, besides being able to affect the lungs can also affect the Body Mass Index. Because every decrease in body mass index occurs there is a decrease in albumin levels.This study aims to determine the relationship between the Body Mass Index and Albumin levels in patients with pulmonary tuberculosis who underwent OAT therapy at Benyamin Guluh Kolaka Hospital. This type of research is observational analytic using the Erba XL 100 cresol green bromine method with accidental sampling technique. The number of samples is 22 people.The results of the study of 22 patients with pulmonary tuberculosis patients who were taking anti-tuberculosis drugs (OAT) at Benyamin Guluh Kolaka Hospital obtained albumin levels in 13 men (59.09%) and 9 women (40.91%). After the correlation test was obtained, the p-value was 0.110 (p> 0.05), the hypothesis (H0) was accepted, namely there was no relationship between body mass index and albumin level in pulmonary tuberculosis patients at Benyamin Guluh Kolaka Hospital. Keywords : Pulmonary Tuberculosis, Albumin
GAMBARAN HASIL UJI SARING HEPATITIS B METODE CHEMILUMINESCENCE IMMUNOASSAY (CHLIA) PADA DARAH DONOR DI UDD PMI KABUPATEN BANYUMAS TAHUN 2022 Sherliana Alfianni; Nurpuji Mumpuni; Reza Iqbal Suhada
Jurnal Analis Kesehatan Kendari Vol. 6 No. 1 (2023): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 6 (1) Desember 2023)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v6i1.262

Abstract

ABSTRACT Background: Hepatitis B is a liver infection caused by the Hepatitis B Virus (VHB) which can lead chronic infection and liver cancer, making it at high risk of death. The prevalence of Hepatitis B in 2022 at UDD PMI Banyumas Regency was recorded at 141 donors with a percentage of 0.21% declared Reactive (R) to Hepatitis B from a total of 68,380 donors. UDD PMI Banyumas Regency has been certified CPOB (Good Manufacturing Practices) since 2019 and is headed by doctor Winda Astuti Taruno. Objective: To determine the description of the results of the Hepatitis B screening test with the Chemiluminescence ImmunoAssay (ChLIA) method at UDD PMI Banyumas Regency in 2022. Method: This study is a quantitative descriptive study with a retrospective time approach, namely the results of the Hepatitis B screening test identified with blood donor characteristics, such as gender, age and blood type in UDD PMI Banyumas Regency in 2022. Results: The number of blood carried out by Hepatitis B screening tests during 2022 was 68,380 blood samples, the most samples were in March at 6,387 (9.30%) and the least in May at 4,853 (7.10%). The results of the Hepatitis B screening test obtained Reactive results against Hepatitis B as much as 141 (0.21%) and Non-Reactive as much as 68,239 (99.79%). Based on the characteristics of donors, the results of the Reactive Hepatitis B (R) screening test in donor blood are more common in women, namely 46 donors (0.24%), in the age group (36-45 years) as many as 50 donors (0.31%), in blood type O as many as 53 (0.20%), and in Rhesus positive as many as 141 (0.21%). The handling of reactive blood in UDD PMI Regency is to conduct a duplex examination, if the results are obtained Reactive (Repeated Reactive), then the blood is destroyed and the donor is referred to an Internal Medicine Specialist at the Hospital for re-examination and counseling for the continuation of the donor. Conclusions: The results of the Hepatitis B screening test on donor blood at UDD PMI Banyumas Regency in 2022 were Reactive Hepatitis B of 0.21% and Non-Reactive Hepatitis B of 99.79%. Reactive Hepatitis B is most common in women, in late adulthood and in blood type O. Reactive blood management is in accordance with the Minister of Health Regulation Number 91 of 2015. Keywords: Hepatitis B; ChLIA method; Blood Donor, UDD PMI Banyumas Regency; 2022 ABSTRAK Pendahuluan: Hepatitis B ialah infeksi hati yang disebabkan oleh Virus Hepatitis B (VHB) yang dapat mengakibatkan infeksi kronis serta kanker hati, sehingga beresiko tinggi terhadap kematian. Prevalensi Hepatitis B pada tahun 2022 di UDD PMI Kabupaten Banyumas tercatat sebanyak 141 pendonor dengan persentase 0,21% dinyatakan Reaktif (R) terhadap Hepatitis B dari total pendonor sebanyak 68.380. UDD PMI Kabupaten Banyumas sudah tersertifikasi CPOB (Cara Pembuatan Obat yang Baik) sejak tahun 2019 dan dikepalai oleh dr. Winda Astuti Taruno. Tujuan: Untuk mengetahui gambaran hasil uji saring Hepatitis B dengan metode Chemiluminescence ImmunoAssay (ChLIA) di UDD PMI Kabupaten Banyumas Tahun 2022. Metode: Penelitian ini merupakan penelitian deskriptif kuantitatif dengan pendekatan waktu retrospektif, yaitu hasil uji saring Hepatitis B yang dikaitkan dengan karakteristik pendonor darah, seperti Jenis kelamin, usia dan golongan darah di UDD PMI Kabupaten Banyumas tahun 2022. Hasil: Jumlah darah yang dilakukan uji saring Hepatitis B selama tahun 2022 didapatkan sebanyak 68.380 sampel darah. Hasil uji saring Hepatitis B menunjukkan hasil Reaktif (R) terhadap Hepatitis B sebanyak 141 (0,21%) dan Non Reaktif (NR) sebanyak 68.239 (99,79%). Berdasarkan karakteristik pendonor hasil uji saring Hepatitis B Reaktif (R) pada darah donor lebih sering terjadi pada jenis kelamin perempuan yakni 46 pendonor (0,24%), pada kelompok usia (36 – 45 tahun) sebanyak 50 pendonor (0,31%), pada golongan darah O sebanyak 53 (0,20%), dan pada Rhesus positif sebanyak 141 (0,21%). Penanganan darah reaktif di UDD PMI Kabupaten yaitu melakukan pemeriksaan secara duplo, jika didapatkan hasil Reaktif (Repeated Reactive), maka darah dimusnahkan dan pendonor dirujuk ke Dokter Spesialis Penyakit Dalam di Rumah Sakit untuk dilakukan pengecekkan ulang dan konseling untuk kelanjutan donornya. Kesimpulan: Hasil uji saring Hepatitis B pada darah donor di UDD PMI Kabupaten Banyumas tahun 2022 yang Reaktif Hepatitis B sebesar 0,21% dan Non Reaktif Hepatitis B sebesar 99,79%. Reaktif Hepatitis B paling banyak pada perempuan, pada usia dewasa akhir dan pada golongan darah O. Penanganan darah Reaktif sudah sesuai Peraturan Menteri Kesehatan Nomor 91 Tahun 2015. Kata Kunci: Hepatitis B; Metode ChLIA; Darah Donor, UDD PMI Kabupaten Banyumas; 2022
UJI DAYA HAMBAT EKSTRAK DAUN KEDONDONG (Spondias dulcis L.) TERHADAP PERTUMBUHAN JAMUR Aspergillus flavus Ani Umar; jumiati jumiati; Viranda Yuniar
Jurnal Analis Kesehatan Kendari Vol. 6 No. 1 (2023): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 6 (1) Desember 2023)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v6i1.281

Abstract

ABSTRACT Different chemical compounds in various plants such as leaves. One of an alternative vegetable material that has the potential to have activity as antifungi is a leaf of kedondong. The leaves have saponin compounds, alkaloids and tannins. This study aims to determine the inhibitor power of leaves kedondong (Spondias dulcis L.) to Aspergillus flavus and to determine the concentration of leaf extract kedondong (Spondias dulcis L.) which effectively inhibits the growth of Aspergillus flavus fungus. Types of research is experiments with the extract of the given kedondong leaf treatment as an inhibitory test againts Aspergillus flavus growth. Kedondong leaf extract obtained by maseration method for 5 days and this research uses Kirby bauer method (diffusion disk). The results showed that there is no clear zone around the disc paper indicating the extract of the kedondong leaf can not be inhibits the growth of Aspergillus flavus with concentrations of 5%, 10%, and 15%. Keywords : Inhibition Zones, Kedondong Leaves, Aspergillus flavus. ABSTRAK Pertumbuhan jamur Aspergillus flavus dapat dihambat oleh kandungan senyawa kimia yang beragam pada berbagai tumbuhan seperti daun. Salah satu alternatif bahan nabati yang berpotensi mempunyai aktivitas sebagai antifungi adalah daun kedondong. Daun tersebut memiliki senyawa saponin, alkaloid dan tannin. Penelitian ini bertujuan untuk mengetahui daya hambat daun kedondong (Spondias dulcis L.) terhadap jamur Aspergillus flavus dan untuk menentukan konsentrasi ekstrak daun kedondong (Spondias dulcis L.) yang efektif menghambat pertumbuhan jamur Aspergillus flavus. Jenis penelitian yang dilakukan adalah eksperimen dengan ekstrak daun kedondong diberikan perlakuan sebagai uji daya hambat terhadap pertumbuhan Aspergillus flavus. Ekstrak daun kedondong diperoleh dengan metode maserasi selama 5 hari dan penelitian ini menggunakan metode Kirby bauer (difusi disk). Hasil penelitian menunjukan bahwa tidak terdapat zona bening disekitar paper disk yang menandakan ekstrak daun kedondong tidak dapat menghambat pertumbuhan Aspergillus flavus dengan konsentrasi 5%, 10%, dan 15%. Kata kunci : Zona Hambat, Daun Kedondong, Aspergillus flavus
GAMBARAN KADAR HEMOGLOBIN DAN INDEKS ERITROSIT SEBAGAI PENANDA ANEMIA PADA KARYAWAN PT. ANTAM DI KECAMATAN POMALAA KABUPATEN KOLAKA PROVINSI SULAWESI TENGGARA Kemal Idris Balaka; Heni Umar; Niar Jumiarti Saadi
Jurnal Analis Kesehatan Kendari Vol. 6 No. 1 (2023): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 6 (1) Desember 2023)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v6i1.282

Abstract

ABSTRACT Anemia is a condition in which the hemoglobin (Hb) level in the blood is less than normal. Anemia is a nutritional problem that is widely found throughout the world that not only occurs in developing countries but also in developed countries Anemia is a condition with hemoglobin levels, hematocrit and red blood cells that are lower than normal values. Hemoglobin functions to deliver oxygen throughout the body and gives color to erythrocyte cells. In addition to hemoglobin the supporting parameters for knowing someone suffering from anemia is to look at the erythrocyte index. The purpose of this study was to determine the description of hemoglobin levels and erythrocyte index as an anemic marker in employees of PT. Antam in Pomalaa District, Kolaka Regency, Southeast Sulawesi Province. This type of is descriptive to determine the description of hemoglobin levels and erythrocyte index as a marker of anemia in employees of PT. Antam where the number of samples was 25 people with the hemotology analyzer method and purposive sampling technique. Based on the research results of research conducted on 25 samples of hemoglobin levels and erythrocyte index as markers of anemia in employees of PT. Antam in Pomalaa District, Kolaka Regency, Southeast Sulawesi Province, obtained normal hemoglonin and erythrocyte index (100%). Keywords: Anemia, Hemoglobin, Erythrocyte Index, Employees PT. Antam ABSTRAK Anemia adalah suatu keadaan dimana kadar Hemoglobin (Hb) dalam darah kurang dari normal. Anemia merupakan masalah gizi yang banyak terdapat di seluruh dunia yang tidak hanya terjadi di negara berkembang tetapi juga di negara maju Anemia adalah keadaan dengan kadar hemoglobin, hematokrit dan sel darah merah yang lebih rendah dari nilai normal. Hemoglobin berfungsi mengantarkan oksigen keseluruh tubuh dan memberi warna pada sel eritrosit. Selain hemoglobin parameter penunjang untuk mengetahui seseorang menderita anemia adalah dengan melihat indeks eritrosit. Tujuan penelitian ini untuk mengetahui gambaran kadar hemoglobin dan indeks eritrosit sebagai penanda anemia pada karyawan PT.Antam di Kecamatan Pomalaa Kabupaten Kolaka Provinsi Sulawesi Tenggara. Jenis penelitian adalah deskriptif untuk mengetahui gambaran kadar hemoglobin dan indeks eritrosit sebagai penanda anemia pada karyawan PT. Antam dimana jumlah sampel sebanyak 25 orang dengan metode hemotology analyzer dan teknik pengambilan sampel purposive sampling. Berdasarkan hasil penelitan yang telah dilakukan terhadap 25 sampel kadar hemoglobin dan indeks eritrosit sebagai penanda anemia pada karyawan PT.Antam di Kecamatan Pomalaa Kabupaten Kolaka Provinsi Sulawesi Tenggara diperoleh nilai kadar hemoglobin dan indeks eritrosit normal (100%). Kata kunci: Anemia, Hemoglobin, Indeks Eritrosit, Karyawan PT. Antam
IDENTIFIKASI Mycobacterium tuberculosis PADA PENDERITA HIV/AIDS DI KOTA KENDARI Darmayanita Wenty; Fracisca Romania Sri Supadmi; Sry Waly Waly
Jurnal Analis Kesehatan Kendari Vol. 6 No. 1 (2023): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 6 (1) Desember 2023)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v6i1.285

Abstract

ABSTRACT Human Immunodeficiency Virus (HIV) is a RNA retrovirus that attacks the human immune system. This virus infects cells that have Cluster of Differentiation 4 (CD4) molecules, especially T lymphocytes that have receptors with high affinity for HIV. HIV virus can cause some opportunistic infections one of them tuberculosis. Tuberculosis is a chronic infection caused mainly by Mycobacterium tuberculosis. This study aims to determine the results of identification of Mycobacterium tuberculosis of people with HIV / AIDS in Kendari City. This study was carried out in the Microbiology Laboratory of Bina Husada Polytechnic Kendari. Type of this study is semi-quantitative with the sampling technique by accidental sampling as many as 10 samples. The examination used Acid Resistant Basil (BTA) and the method used Ziehl Neelsen with the results obtained did not found smear in 100 visual fields (negative) for all samples. Based on the research that has been carried out, it could be concluded that the examination of Mycobacterium tuberculosis in HIV / AIDS sufferers in Kendari City obtained negative results (-) for all samples. Key words: HIV, AIDS, TB, Mycobacterium tuberculosis ABSTRAK Human Immunodeficiency Virus (HIV) merupakan retrovirus golongan RNA yang menyerang kekebalan tubuh manusia. Virus ini menginfeksi sel yang mempunyai molekul Cluster of Differentiation 4 (CD4) terutama limfosit T yang memiliki reseptor dengan afinitas yang tinggi untuk HIV. Virus HIV dapat mengakibatkan beberapa infeksi oportunistik salah satunya tuberkulosis. Tuberkulosis adalah penyakit infeksi kronis terutama disebabkan oleh Mycobacterium tuberculosis. Penelitian ini bertujuan untuk untuk mengetahui hasil identifikasi Mycobacterium tuberculosis pada penderita HIV/AIDS di Kota Kendari. Penelitian ini dilaksanakan di Laboratorium Mikrobiologi Politeknik Bina Husada Kendari. Jenis penelitian ini adalah semi kuantitatif dengan teknik pengambilan sampel secara accidental sampling sebanyak 10 sampel. Pemeriksaan menggunakan pewarnaan Basil Tahan Asam (BTA) dengan metode Ziehl Neelsen dengan hasil yang diperoleh tidak ditemukan BTA dalam 100 lapang pandang (negatif) untuk semua sampel. Berdasarkan penelitian yang telah dilakukan diperoleh kesimpulan bahwa pemeriksaan Mycobacterium tuberculosis pada penderita HIV/AIDS di Kota Kendari diperoleh hasil negatif (-) untuk semua sampel. Kata Kunci : HIV, AIDS, TB, Mycobacterium tuberculosis
GAMBARAN JUMLAH KOLONI BAKTERI PADA PENGGUNA GIGI TIRUAN (LEPAS PASANG) DI KOTA KENDARI Sri Aprilianti Idris; Desih Welliam; Citra Ayunis
Jurnal Analis Kesehatan Kendari Vol. 6 No. 1 (2023): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 6 (1) Desember 2023)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v6i1.288

Abstract

ABSTRACT Penggunaan gigi tiruan yang lepas pasang dapat meningkatkan risiko pertumbuhan bakteri. Hal ini dikarenakan celah antara gigi tiruan dan gusi dapat menjadi tempat bagi bakteri untuk berkembang biak. Pengguna gigi tiruan disarankan untuk membersihkan dan merawat gigi tiruan mereka secara teratur dengan menyikat, membersihkan, dan merendam gigi tiruan dalam larutan pembersih yang direkomendasikan. Tujuan : Untuk mengetahui jumlah koloni bakteri pada pengguna gigi tiruan (lepas pasang) Di BTN Dewi Bunga Poasia 2 kota Kendari. Metode Penelitian kualitatif dapat dipahami sebagai metode penelitian eksperimental. Hasil : Hasil penelitian pada perlakuan pertama pada setiap sampel yang menggunakan gigi tiruan dilakukan isolasi sampel pada media NA di inkubasi 1 x 24 jam pada suhu 37℃, tahap kedua dilakukan perhitungan jumlah koloni bakteri dengan menggunakan alat CFU, dan tahap ketiga dilakukan pewarnaan gram pada sampel ditemukan hasil bakteri gram positif. Kesimpulan : Berdasarkan hasil penelitian yang telah dilakukan dapat disimpulkan bahwa : Pada pengenceran 10-6 sampel 1 terdapat 258 koloni bakteri, sampel 2 terdapat 93 koloni bakteri, sampel 3 terdapat 71 koloni bakteri, sampel 4 terdapat 25 koloni bakteri, sampel 5 terdapat 19 koloni bakteri, sampel 6 terdapat 905 koloni bakteri, sampel 7 terdapat 700 koloni bakteri, sampel 8 terdapat 511 koloni bakteri, sampel 9 terdapat 407 bakteri, sampe 10 terdapat 291 bakteri. Kata kunci: Jumlah koloni, bakteri, gigi tiruan ABSTRAK The use of removable dentures can increase the risk of bacterial growth. This is because the gap between the denture and the gums can be a place for bacteria to multiply. Denture wearers are advised to clean and care for their dentures regularly by brushing, cleaning, and soaking the dentures in the recommended cleaning solutions. Objective: To determine the number of bacterial colonies in denture users (removable) at BTN Dewi Bunga Poasia 2, Kendari city. Qualitative research methods can be understood as experimental research methods. Results: The results of the research in the first treatment for each sample using dentures were carried out by isolating the sample on NA media in 1 x 24 hour incubation at 37 ℃, the second stage was carried out by calculating the number of bacterial colonies using a CFU tool, and the third stage was carried out gram staining on the samples found gram positive bacteria. Conclusion: Based on the results of the research that has been done, it can be concluded that: at dilution of 10-6, sample 1 contains 258 bacterial colonies, sample 2 contains 93 bacterial colonies, sample 3 contains 71 bacterial colonies, sample 4 contains 25 bacterial colonies, sample 5 contains 19 bacterial colonies, sample 6 contains 905 bacterial colonies, sample 7 contains 700 bacterial colonies, sample 8 contains 511 bacterial colonies, sample 9 contains 407 bacteria, sample 7 contains 10 there 291 bacteria. Key words: Colonies, Bacterial, Dentures