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Annales Bogorienses
Published by Badan Riset dan Inovasi Nasional
ISSN : 05178452     EISSN : 24077518     DOI : https://doi.org/10.55981/ann.bogor
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Annales Bogorienses aims to disseminate high-quality scientific research in the field of life sciences, with a strong emphasis on advancing knowledge and applications in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. The journal serves as a platform for researchers, academicians, and practitioners to share original findings, innovative methodologies, and critical reviews that contribute to scientific progress and sustainable development. The journal covers research in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. It publishes original research articles, reviews, and short communications, and is committed to rigorous peer review and open access for the widest possible dissemination of scientific knowledge.
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Articles 189 Documents
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In Vitro Ruminal Fermentation and Degradability of Rice Husk on Rice Bran Substitution Fidriyanto, Rusli; Ridwan, Roni; Astuti, Wulansih Dwi; Rohmatussolihat, Rohmatussolihat; Nurul Fitri Sar; Watman, Muh; Widyastuti, Yantyati
Annales Bogorienses Vol. 24 No. 1 (2020): Annales Bogorienses
Publisher : BRIN

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Rice bran is a by-product of the rice milling process and has been well used as livestock feed. Rice bran is often adulterated with rice husk. The objective of this study was to evaluate the in vitro ruminal fermentation characteristics of rice bran with various compositions of rice husk and assess the relationship between rice husk addition and rice bran quality. The experiment was arranged in a completely randomized design with rice husk addition as a factor and three replications. Data of proximate value, gas production, ruminal degradability, and volatile fatty acid production were analyzed by analysis of variance. Moreover, significant effects of each treatment in the in vitro fermentation were further analyzed by Duncan's multiple range test (P<0.05). It was shown that the addition of rice husk to rice bran could increase acetic acid level, but it reduced potential gas production, gas production rate, organic matter and dry matter digestibility, and propionic acid level. Interestingly, the linear regression of dry matter digestibility, organic matter digestibility, and potential gas production showed the high adjusted R2 values. Moreover, this study also revealed that 10% of rice husk substitution on rice bran could significantly reduce the dry matter digestibility.
Potency of Curcuma aeruginosa as A Chemopreventive Candidate Agent on 7,12-Dimethylbenz[a]anthracene (DMBA)-Induced Rat Spleen Sulfianti, Asri; Hasanah, Nur; Wibowo, Agung Eru; Agustini, Kurnia; Artika, I Made
Annales Bogorienses Vol. 23 No. 2 (2019): Annales Bogorienses
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Present investigation shows that the extract of C. aeruginosa attenuates DMBA-induced spleen carcinogenesis in Wistar rats. Three-week female Wistar rats were treated with three different C. aeruginosa extract doses (CA1: 40 mg/200 g body weight, BW; CA2: 80 mg/200 g BW; CA3: 160 mg/200 g BW) and were induced with DMBA after one-week administration of these doses. A commercial immunostimulant, and DMBA only were also given to each group as positive and negative control, respectively. The development of tumors was evaluated by investigating the incidence of tumor and tumor multiplicity during the experiment. Spleen mass index and histological parameters such as white pulp, centrum germinativum, and marginalis zone were also examined. Based on our study, the administration of C. aeruginosa extract during and after carcinogen induction gave several impacts on rat carcinogenesis. At the extract dose of 80 mg/200 g BW, tumor incidence of animals were least (P<0.05). However, all doses did not show any effect to the spleen mass index, though the highest dose (160 mg/200 g BW) was found to cause changes in white pulp and marginalis zone boards. This trend indicates that it takes higher dose to cause an immune response effect reaching the organs.
Laccase and Manganese Peroxidase (MnP) Activities in the White-Rot Fungus Trametes hirsuta in Response to Aromatic Compounds Nuryana, Isa; Ilmiah, Zidny; Andriani, Ade; Yopi, Yopi
Annales Bogorienses Vol. 23 No. 2 (2019): Annales Bogorienses
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The current interest in exploring white-rot fungi has been concentrated in increasing their ligninolityc enzyme production such as laccase and manganese peroxidase (MnP) due to the great value in industrial application. The presence of appropiate inducers can enhance the enzyme production. Hence, the aim of the study was to investigate the activity of laccase and MnP in response to various aromatic compounds. The fungus Trametes hirsuta was cultured in Glucose Yeast Peptone (GYP) broth (pH 5.0) with the addition of 0.5% of sorghum biomass as substrate. The cultures were then incubated on rotary shaker at 150 rpm at 27oC. The 7-day-old cultures were then supplemented with different aromatic compounds, namely caffeic acid, gallic acid, syringic acid, trans-cinnamic acid, vanillin, and veratryl alcohol. Our results demonstrated that veratryl alcohol, gallic acid and vanillin gave an inductive effect on the value of laccase and MnP activities, with vanillin showing the highest induction. On the contrary, the level of laccase and MnP activities remained low in the presence of aromatic compound such as syringic acid, trans-cinnamic acid and caffeic acid. To conclude, our study reveals that aromatic compounds can be potential as inducers and may contribute to the improvement of laccase and MnP productivity by T. hirsuta.
Development of Mice Embryo (Mus musculus L.) after Closed Pulled Straw Vitrification in CZB Medium Gunawan, Muhammad; Kaiin, Ekayanti Mulyawati; Rusherdiannita, Raden Cindy; Alipin, Kartiawati
Annales Bogorienses Vol. 23 No. 2 (2019): Annales Bogorienses
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The objective of our present experiment was to investigate the effects of closed pulled straw (CPS) vitrification on the viability and development of mouse embryo. The experiment was arranged according to completely randomized design (CRD) consisting of 4 treatments, namely not vitrification (NV) is control, CPS 1 (mCZB Hepes + 20% Bovine Serum Albumin (BSA) +0.5 M sucrose + 10% EG + 10% DMSO), CPS 2 (mCZB Hepes + 20% BSA + 0.5 M sucrose + 15% EG + 15% DMSO), and CPS 3 (mCZB Hepes + 20% BSA +0.5 M sucrose + 20% EG + 20% DMSO) with 6 replications. The viability of embryos (%) was determined after 24 - 72 h of the culture period, while we also observed the percentage of embryos reaching the blastocyst stage (early blastocyst, expanded, hatching, and hatched). As a result, the treatments did not give a significant difference in the viability of embryos (P<0,05) but showed significant effects on embryo development (P<0,05). Furthermore, this present work conclusively found that CPS vitrification in CZB medium with cryoprotectants ethylene glycol (EG) and dimethyl sulfoxide (DMSO) noticeably influenced the development of mice embryo to reach the blastocyst stage, but showed no remarkable difference in the viability of embryo after culture for 24 – 72 h.
Cytotoxic Activity of Flavonoid Compounds Isolated from Tetrameles Nudliflora Leaves Megawati, Megawati; Darmawan, Akhmad; Fajriah, Sofa
Annales Bogorienses Vol. 23 No. 2 (2019): Annales Bogorienses
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Tetrameles nudliflora is one of the endemic plants in Wallace region, especially in Mekongga Forest, Southeast Sulawesi, Indonesia. As part of our project in the field of natural drug discovery based on local bioresources, this study aimed to isolate secondary metabolite compounds from the ethyl acetate fractions of the leaves of T. nudiflora and evaluate their cytotoxic activities against breast carcinoma cancer lines, MCF-7. The methods used were various chromatography techniques such as vacuum column chromatography, gravitation column chromatography, thin layer chromatography, preparative thin layer chromatography, and re-crystallization using two different organic solvents. In the present study, two flavonoid compounds, kaempferol (1) and quercetin (2) were identified. The structures of 1-2 were determined using serial spectroscopic techniques, including mass spectrometry, one- and two-dimensional nuclear magnetic resonance. Moreover, using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay method, both compounds showed in vitro cytotoxic activity IC50 of 34.162 and 133.148 μg mL-1, respectively against MCF-7 cell lines.
Field Evaluation of Conde Progenies with Early Heading Date and Resistance to Bacterial Leaf Blight Fatimah, Fatimah; Prasetiyono, Joko; Dadang, Ahmad; Tasliah, Tasliah; Masumah, Masumah
Annales Bogorienses Vol. 23 No. 2 (2019): Annales Bogorienses
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Heading date is one of key traits in rice adaptation to different environments. Rice with early maturity is very useful for increasing rice production extensively especially in Indonesia. The improvement of rice variety Conde for heading date and resistance to bacterial leaf blight (BLB) was developed through Marker Assisted Backcrossing approach (MAB). Xa7 derived from Conde as recipient parents for resistance to BLB and Hd2 gene derived from Nipponbare as a donor parent for heading date. The aim of this research was to evaluate the performance of heading date and agronomic traits of 85 BC2F4 lines in two field stations, Sukamandi (West Java) and Maros (South Sulawesi). Molecular analysis of Xa7 on chromosome 6 and Hd2 gene on chromosome 7 and agronomic performance was evaluated. Foreground analysis resulted 41% for Xa7 and 66.7% for Hd2 gene in homozygous condition. The majority of lines have similar heading date and yield with Conde and more resistance to bacterial leaf blight (BLB) than Conde. Six lines flowered earlier 5 days than Conde in Sukamandi and three lines flowered earlier 3 days in Maros. Twelve lines with flowered earlier than Conde, resistance to BLB and similar yield with Conde were selected for background analysis resulted 93% genome recovery. These lines would be further evaluated in preliminary yield trail for their stability.
Diversity and Antimicrobial Activity of Lichens-Associated Actinomycetes in Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) Indonesia Susanti, Agustina Eko; Ratnakomala, Shanti; Mangunwardoyo, Wibowo; Lisdiyanti, Puspita
Annales Bogorienses Vol. 23 No. 1 (2019): Annales Bogorienses
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Bioprospecting has developed to all biological taxa including procaryotic. Actinomycetes become interesting procaryotic because of the ability to produce important secondary metabolite for human life. Actinomycetes are known as the largest antibiotic producer that has a broad range habitat. Some research has been done to find new antibiotic from the various habitat of actinomycetes. One of the interesting habitats of actinomycetes which never been explored in Indonesia is lichens... Lichens as the symbiotic structure of alga and fungi areknown as the ecological niche of various kinds of microorganisms including actinomycetes. Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) have various species of trees as the habitat of lichens. These areas are known as one of the research locations to explore the biodiversity of Indonesia. The aims of this research is to study the diversity and antimicrobial potency of actinomycetes isolated from 10 lichen samples with various type of thallus; crustose, fructose and foliose. Lichen samples were grown on the bark of 9 trees species in CSC and CBG. Isolation process used three agar media; HV, YIM6 and YIM711 with cycloheximide and nalidixic acid. Molecular identification based on 16S rRNA gene sequence. Antimicrobial activity was tested to 65 isolates by agar diffusion method to Bacillus subtilis BTCC B.612, Escherichia coli BTCC B.614, Candida albicans BTCC Y.33, Staphylococcus aureus BTCC B.611, Micrococcus luteus BTCC B.552. Isolation process retrieved 125 isolates with the highest number grow on HV agar medium. Based on the sample, the highest number of actinomycetes were isolated from crustose lichen attached on the bark of Averrhoea carambola. A total 69 isolates were identified as the genera Actinoplanes, Amycolatopsis, Angustibacter, Kribbella, Micromonospora, Mycobacterium, and Streptomyces. The screening process showed 24 isolates have antimicrobial activity, with the highest inhibitory activity against Micrococcus luteus BTCC B.552.
Morphology and Molecular Characterization of Newly Isolated Microalgae Strain Chlorella volutis LIPI13-WKT066 from Wakatobi Islands and Its Potential Use Rahman, Delicia Yunita; Praharyawan, Swastika; Fadiyah, Farizul; Raharjo, Sapto; Susilaningsih, Dwi
Annales Bogorienses Vol. 23 No. 1 (2019): Annales Bogorienses
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Morphology and molecular characterization of microalgae isolated from Wakatobi Marine National Park was conducted. An understanding of the characteristics of morphology, molecular, as well as metabolites profile of the microalgae species is potentially useful for its future application. The primary aim of this study was to isolate, identify and characterize the microalgae strain isolated from Wakatobi Marine National Park labeled as LIPI13-WKT066 with the emphasis on the evaluation of amino acid content as a basis for its health application. Morphological observation under the microscope and molecular identification suggested that the microalgae strain of LIPI13-WKT066 belong to the strain under species of Chlorella volutis. Metabolite characterization of the microalgae strain showed that the content of protein (11.9%), lipid (12.4%) and carbohydrate (4.7%) was in the regular range. Further analysis of its amino acid content revealed the potency of the microalgae strain to be used as antihypertensive agent.
Enzyme Production from Cassava Peels by Aspergillus Awamori KT-11: The Making of Natural Sweetener from Several Tubbers Melliawati, Ruth; Rahman, Farida
Annales Bogorienses Vol. 23 No. 1 (2019): Annales Bogorienses
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The use of cassava (Manihot esculenta Crantz) peel for enzyme production has not been widely used. The purpose of this study was to produce complex amylase enzymes from cassava peel by A. awamori KT-11 and apply them in the manufacture of natural sweeteners. Enzyme production is carried out on red and white cassava peel. Media of cassava peel sterilized, inoculated with 1% A. awamori KT-11, incubated for 5 days, then dried at 50°C and mashed. Making sugar is done on cassava flour, sweet potato ( Ipomoea batatas L), taro (Colocasia esculenta) and cocoyam (Xanthosoma sagittifolium) with different concentrations of 10%, 15%, 20%, and 15% and 20% enzyme concentrations. The hydrolysis process is carried out for 3 days at 60°C. The enzyme activity in red cassava peel was 405,006 U/mL and white cassava peel was 321,239 U/ml. The sugar produced in cassava, taro, sweet potato, and Cocoyam was 101.38 mg/mL, 81.18 mg/mL, 55.929 mg/mL, and 42.874 mg/mL, respectively. The results of TLC showed that cassava and taro sugar contain maltose, lactose and glucose, sweet potatoes contained glucose and dextrin and Cocoyam containing fructose. The sweetness level of sugar from cassava, taro, sweet potato and Cocoyam is 14 brix, 12 brix, 9 brix and 9 brix, respectively.
Overexpression of OsHox-6 Gene Enhanced Tiller Number in Rice But Induced Yield Penalty Rahmawati, Syamsidah; Chairunnisa, Chairunnisa; Erdayani, Eva; Nugroho, Satya; Estiati, Amy
Annales Bogorienses Vol. 23 No. 1 (2019): Annales Bogorienses
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OsHox-6, belongs to the transcription factor homeodomain leucine zipper (HD-Zip) protein sub-family I, has unknown function. This study was aimed to characterize the phenotypes of two homozygous transgenic rice lines (S29-62-2 and S.40.4-158-1) containing an extra copy of OsHox-6 gene under the control of a rice constitutive promoter, OsLEA3, and to evaluate their tolerance to water stress. A real-time quantitative PCR (qRT-PCR) showed that the transcript expression of OsHox-6 gene in the transgenic lines increased 5-10 folds under a normal irrigation and 10-20 folds after exposure to water stress conditions as compared to its wild type control. Transgenic plants overexpressing OsHox-6 exhibited phenotypic alteration at the normal irrigation by inducing tiller formation, suggesting a decrease in the apical dominance. Transgenic plants also showed significant enhancement in the total grain number, however, the number of empty grains also increased significantly (~16-22%). After imposed to the water stress, the number of empty grains in the transgenic lines was even higher (up to 83% in average). Furthermore, observations on the water loss rates, relative water contents and drought resistance indices (DRI) suggested that the overexpression of OsHox-6 did not significantly increase tolerance to water stress. Further research is required to reveal the detailed mechanisms of OsHox-6 in response to water and other abiotic stresses.

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