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Jurnal Bioteknologi & Biosains Indonesia
Published by Badan Riset dan Inovasi Nasional
ISSN : 24422606     EISSN : 2548611X     DOI : https://doi.org/10.55981/jbbi
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general. JBBI terbit dua kali setahun dan menyediakan media penerbitan ilmiah bagi para peneliti, insinyur, praktisi, akademisi, dan pemerhati di bidang yang terkait dengan bioteknologi dan biosains. Jurnal ini menerima naskah asli hasil penelitian, naskah ulasan, studi kasus, dan komunikasi singkat. Naskah yang diterbitkan adalah peer-review oleh tidak kurang dari dua orang penelaah, dan mencakup berbagai cabang bioteknologi yang terkait dengan bidang pertanian, industri, kesehatan, lingkungan, bioinformatika, serta ilmu kehidupan pada umumnya.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 21 Documents
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Search results for , issue "Vol. 12 No. 1 (2025)" : 21 Documents clear
FROZEN SEMEN CHARACTERISTICS OF BALI BULL IN DIFFERENT AGE GROUPS Witri, Brilla Widya; Arifiantini, Iis; Prasetyaningtyas, Wahono Esthi; Karja, Ni Wayan Kurniani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10087

Abstract

One factor that affects frozen semen characteristics is bull age. Increasing age induces changes in sperm that reduce frozen semen quality. This study aimed to evaluate the frozen semen characteristics of Bali bulls aged 3, 8, and 13 years. Frozen semen was derived from fresh semen with sperm motility > 70%. The frozen semen characteristics tested included motility, viability, plasma membrane integrity, acrosome integrity, and sperm abnormalities. Sperm motility was analyzed by computer-assisted sperm analysis (CASA). Viability was assessed using eosin-nigrosin staining; plasma membrane integrity was assessed using the hypoosmotic swelling test (HOST); acrosome integrity was assessed using Giemsa staining; and sperm abnormalities were assessed using Williams staining. The plasma membrane integrity of the 13-year-old group was significantly lower (P < 0.05) than that of the younger age groups. However, there were no significant differences (P > 0.05) in the other parameters. We conclude that age affects the integrity of the sperm plasma membrane in frozen semen from Bali bulls.
THE DIVERSITY AND CHARACTERISATION OF CELLULOLYTIC BACTERIA ISOLATED FROM VARIOUS CONDITIONS OF EMPTY FRUIT BUNCHES IN OIL PALM PLANTATION Agam Pamungkas, Edo; Madusari, Sylvia; Putri, Halida Adistya; Rosita, Risa
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10238

Abstract

The utilisation of oil palm empty fruit bunches (EFB) remains underexplored. Harnessing cellulolytic microorganisms for the production of cellulase enzymes offers sustainable approach to addressing waste management challenges while aligning with the principles of the Sustainable Development Goals (SDGs) and address waste management challenges. This study aims to isolate, characterize, identify, and test the potential cellulase activity of cellulolytic bacteria from EFB taken from three different locations: PO code from organic fertilizer plantations (POU1, POU2, POU3), PL code from oil palm plantations (PLU1, PLU2, PLU3), and PK code from Sulung mills (PKU1, PKU2, PKU3). This study used three isolated cultures in its testing. The research process includes sample preparation, bacterial isolation, gram staining, catalase test, hypersensitivity test, DNA amplification, bioinformatics analysis and cellulase activity analysis. The results of the bacterial isolation obtained 28 colonies. The results of the characterisation were all 3 non-pathogenic bacterial isolates, with a positive catalase test. The result of staining Gram-negative with bacilli-shaped bacteria. The amplification results obtained a band size of 1500 bp. The results of the identification obtained the species Aeromonas enteropelogenes, Nitrosomonas stercoris, and Methylobacillus caricis. The results of phylogenetic analysis showed low homology. Cellulase activity of six positive isolates with medium ability isolates code POU3 (1.3), PLU2 (1.0), PLU3 (1.0); low isolates POU1 (0.2), POU2 (0.2), PLU (0.8) and 3 negative isolates no enzyme activity PKU1 (-1), PKU2 (-1), and PKU3 (-1).
IDENTIFICATION OF KEY NITROGEN USE EFFICIENCY-RELATED GENES IN OIL PALM USING BIOINFORMATICS APPROACHES Permatasari, Galuh Wening; Setiowati, Retno Diah; Wening, Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10477

Abstract

Efficient nitrogen use is crucial for maximizing oil palm yield while reducing environmental impact. Poor nitrogen utilization causes excessive growth and nutrient loss. This study uses bioinformatics to identify key genes linked to nitrogen use efficiency, providing insights for genetic improvement and sustainable cultivation.Protein-protein interaction (PPI) networks, functional enrichment, and structural modeling were employed to uncover candidate genes regulating nitrogen uptake and metabolism. Sixty-two nitrogen use efficiency associated genes from rice (Oryza sativa) were analyzed via BLASTp against the E. guineensis genome (NCBI), selecting those with >80% similarity. PPI networks were constructed using STRING-db and analyzed in Cytoscape v3.7.1. Functional enrichment (Gene Ontology) and structural analysis (AlphaFold, PyMol v2.5.4) were performed. Twelve nitrogen use efficiency related genes were identified, with CESA4, CESA7, and CESA9 emerging as key regulators based on high degree and betweenness values in PPI analysis. These genes are linked to plant cell wall biosynthesis. Structural analysis showed high similarity to rice homologs, with RMSD values of 0.338 Å (CESA4) and 0.396 Å (CESA9), indicating strong conservation area. Their structural relevance suggests they are promising targets for molecular breeding marker to enhance nitrogen utilization and sustainability in oil palm.
BLOOD SMEAR EXAMINATION AND DIFFERENTIAL COUNT IN RATS WITH ESCHERICHIA COLI ESBL AND KLEBSIELLA PNEUMONIAE CARBAPENEMASE INFECTIONS Savitri, Lisa; Kasimo, Elfred Rinaldo; Ihsan, Kharisul; Faizal, Imam Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10708

Abstract

Sepsis is an irregular body response to severe infection, triggering uncontrolled inflammation that can lead to extensive tissue damage. It can progress to septic shock with multiple organ failure, resulting in death if left untreated. Laboratory examinations, such as leukocyte differential count in hematology, help understand the distribution pattern of white blood cells associated with health conditions. Laboratory research was conducted on mice injected with E. coli ESBL or K. pneumoniae carbapenemase. After 24 hours, observations were made on apoptosis in the spleen and liver of mice. Mouse blood was processed to count white blood cell types with a differential count. The results were analyzed to compare the control group with the bacterial infection groups of E. coli ESBL and K. pneumoniae carbapenemase. The research results indicate that the neutrophil count in the E. coli ESBL group is still within the normal range and lower (44.5±1.915%) compared to the K. pneumoniae carbapenemase group (55.75±8.342%). Similarly, the lymphocyte count in the E. coli ESBL group is within the normal range and lower (77.5±3.109%) compared to the K. pneumoniae carbapenemase group (91.25±7.588%). This highlights the crucial role of neutrophils and lymphocytes in responding to severe bacterial infections such as K. pneumoniae carbapenemase. Previous studies indicate neutrophilia and lymphocytopenia as markers of severe bacterial infections. Neutrophils are the primary defense against bacterial infections and can be rapidly recruited to the infection site, while specific infections can trigger prolonged neutrophil recruitment from hematopoietic tissues.
DECOLORIZATION OF TEXTILE DYES WITH CRUDE LIGNINOLYTIC ENZYMES FROM Coriolopsis caperata Agnestisia, Retno; Karelius, Karelius; Oksal, Efriyana; Nion, Yanetri Asi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10931

Abstract

The textile industry is a major contributor to global water pollution, releasing an estimated 280,000 tons of synthetic dyes annually into aquatic ecosystems. These dyes, particularly azo compounds, are often toxic, mutagenic, and resistant to conventional wastewater treatment methods. Microbial enzymatic degradation, especially by white-rot fungi, offers a promising eco-friendly alternative. This study aimed to optimize the production of ligninolytic enzymes—Laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP)—from Coriolopsis caperata, and assess their efficiency in degrading two azo dyes: Reactive Red 21 (RR21) and Reactive Orange 107 (RO107). The fungus, isolated from the Peat Swamp Forest in Sebangau, Central Kalimantan, was cultured in a modified glucose-peptone medium enriched with veratryl alcohol. The optimization parameters included variations in time, dye concentration, and the addition of hydrogen peroxide (H₂O₂). Enzyme activity was quantified spectrophotometrically, and dye decolorization was assessed over time at different dye concentrations. Among the enzymes, Lac showed the highest activity (4938.05 U/L), followed by LiP (995.26 U/L) and MnP (246.47 U/L). These values notably exceed several previously reported benchmarks for fungal enzyme activity. RO107 demonstrated greater susceptibility to enzymatic degradation, with 83.71% decolorization achieved at 24 hours, while RR21 reached 65.71% at 48 hours. The addition of 1 mM H₂O₂ significantly enhanced decolorization, increasing RR21 and RO107 removal to 95.71% and 99.30%, respectively. These results underscore the oxidative synergy between H₂O₂ and ligninolytic enzymes, particularly LiP and MnP. Overall, the study demonstrates the potential scalability of C. caperata-based enzymatic treatment systems for textile effluent bioremediation, supporting compliance with environmental discharge regulations and contributing to sustainable wastewater management.
THE INFLUENCE OF PHYSICOCHEMICAL PARAMETERS ON THE ABUNDANCE OF MACROZOOBENTHOS IN KUNGKILAN RIVER, SOUTH SUMATERA Teristiandi, Novin; Yuliana, Meta
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11244

Abstract

Macrozoobenthos are effective bioindicators for assessing freshwater ecosystem health. This study examines the influence of physicochemical parameters and spatial-temporal variation on macrozoobenthic abundance in the Kungkilan River, South Sumatera. Sampling was conducted at upstream, midstream, and downstream sites. Generalized Linear Mixed Model (GLMM) analysis revealed that water depth and transparency had significant positive effects, while iron (Fe) in sediment had a negative effect. Random effects analysis showed that site-level variability (variance = 0.680) was far greater than temporal variability, indicating that spatial differences—especially in the midstream zone—were the dominant drivers of community structure. These findings highlight the importance of localized conservation efforts and reinforce the role of macrozoobenthos as reliable indicators of river health in tropical ecosystems.
IDENTIFICATION AND CHARACTERIZATION OF MICROPLASTIC DEGRADING BACTERIA IN THREE LANDFILLS OF LAMPUNG PROVINCE Hening, Jeni Latri; Kamelia, Marlina; Kesuma, Andri Jaya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11318

Abstract

Microplastics are durable and contain harmful compounds that can be absorbed into the soil and enter the food chain, posing a risk to human health and the environment. One of the efforts to reduce its impact is to utilize bacteria as biodegradation agents. The purpose of this study was to isolate and characterize microplastic degrading bacteria from three landfills in Lampung Province, and determine their degradation rate. The research method used a quantitative descriptive approach, including the isolation stage with the pouring technique, degradation tests using Polystyrene, Polyethylene Terepthalate, and Polyethylene measuring 1x1 cm and identification using Vitek-2. The results of the study from nine bacterial isolates that were successfully purified, three of them showed the highest ability to degrade. The weight reduction result on PS plastic sample was 22.2% by Bacillus circulans, PET sample was 15.9% by Pandoraea spp. and PE sample was 36.2% by Pseudomonas aeruginosa bacteria.
THE EFFECT OF POST-PRODUCTION SHELF TIME ON THE ANTIOXIDANT ACTIVITY OF SOY MILK Fadhillah; Nazip, Khoiron; Meilinda
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11320

Abstract

This study was conducted to analyze the effect of post-production storage time on antioxidant activity in soy milk. Antioxidants are compounds that can ward off free radicals, so it is important to know their stability over time. The storage time used in this study was 1, 4, 7, 10, and 13 hours post-production. Antioxidant activity testing was carried out using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Qualitatively, the color change of the DPPH solution was observed from purple to light yellow, while quantitatively the absorbance value was measured using UV-Vis Spectrophotometry. The inhibition value and IC50 were calculated to determine the strength of antioxidant activity in soy milk. The results showed that soy milk with a storage time of 1 hour had the best antioxidant activity with an IC50 value of 28.19 μg/ml which was classified as very strong. At 4 hours of storage time, the IC50 value of 37.28 μg/ml is classified as moderate, while at 7 hours and 10 hours the IC50 values ​​were 146.95 μg/ml and 119.38 μg/ml, respectively, which are also classified as moderate. At 13 hours, the antioxidant activity decreased drastically with an IC50 value of 228.90 μg/ml, which is classified as very weak. The antioxidant activity of soy milk decreased with increasing storage time. Factors such as temperature, light, and components of soy milk contribute to the degradation of antioxidant activity. Therefore, consumption of soy milk should be done within a shorter time after production to obtain optimal antioxidant benefits.
EFFECTS OF ZINC SUPPLEMENTATION ON REPRODUCTIVE PERFORMANCE IN CATTLE: A REVIEW Akil, Syahrir; Damayanti, Erni; Samsudewa, Daud; Hapila, Ashariah; Iskandar, Hikmayani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11335

Abstract

Zinc is a vital micronutrient involved in various reproductive processes in cattle. This review aims to evaluate the effects of zinc supplementation on cattle reproductive performance by analyzing research articles sourced from Scopus and PubMed databases. Findings indicate that zinc supplementation, provided in either organic or inorganic forms, positively influences reproductive parameters. In bulls, zinc enhances sperm motility, viability, and ejaculate volume. In female cattle, supplementation modulates endocrine hormone levels during both pre-calving and post-calving periods. Moreover, appropriate zinc dosages and supplementation durations contribute to improved reproductive efficiency in cows. These results support the conclusion that zinc supplementation is effective in enhancing reproductive performance in cattle.
VITAMIN C SUPPLEMENTATION IN EGG YOLK CITRATE EXTENDER OPTIMIZES BANGKOK ROOSTER SEMEN QUALITY DURING COLD STORAGE Ananda; Faizal, Noval Jumatul; Jaswandi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11346

Abstract

This study aimed to evaluate the effect of different vitamin C concentrations (0, 0.1, 0.2, and 0.3 g/100 mL) in egg yolk citrate extender and storage time (0, 24, 48, 72, and 96 hours) on the quality of Bangkok rooster semen stored at 5°C. Using a split-plot-in-time design with four replications, semen quality was assessed for motility, viability, abnormality, intact plasma membrane (IPM), and longevity. Results showed a significant interaction (P<0.01) between vitamin C concentration and storage time on motility, and significant effects (P<0.05) on viability, abnormality, and IPM. The 0.2 g vitamin C dose preserved >40% motility and >50% viability for 96 hours and extended sperm longevity up to 11.25±0.95 days. These findings suggest that 0.2 g/100 mL vitamin C is the optimal concentration for preserving rooster semen quality during short-term cold storage.

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